JWSS - Journal of Water and Soil Science

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Methods of seedbed preparation affect establishment, growth and yield of crops via manipulating physical and chemical properties of soil. These effects on wheat-mungbean double-cropping have not been studied in Isfahan. Therefore, the influences of various seedbed preparation methods on soil physical properties and nutrients content and on establishment of mungbean (experimental line 1-61-16) were studied in a wheat-mungbean double-cropping system during 1996 at the Agricultural Research Station, Isfahan University of Technology. Two residue management treatments (burned and unburned) along with four tillage systems (moldboard plow, disk, khishchee and no-till) were laid out in a strip plot design within a randomized complete block design with four replications. Bulk density and penetrability of soil at various depths were not affected by residue management at emergence. However, at the time of flowering, bulk density at 0-15 cm soil depth was lower and soil penetrability at 3-15 cm depth was higher in unburned treatment due to mineralization. Rate and coefficient of emergence of seeds were lower in unburned residue treatment. Soil bulk density at 0-30 cm depths was lower and penetrability at 3-21 cm depth was higher with moldboard plowing at both emergence and flowering time. In addition, lower soil N, P and K content at 0-15 cm depth at emergence and flowering time was obtained with moldboard plowing. Rate and coefficient of emergence were strongly lower in no-till treatment. Based on these results and considering sustainable agricultural goals, incorporating residue with disk tillage might be advantageous under conditions similar to this experiment.

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Germination of strawberry seeds from self- and cross pollination of 4 commercial cultivars were evaluated under mist and in vitro cultures. The study was conducted in McGill University in Quebec, Canada, during 1994 and 1995. Self-pollination of Chambly and Redcoat cvs., and cross pollination of Oka × Chambly as well as Redcoat × Veestar and their reciprocals were used. At maturity, fruits were harvested and their seeds were separated. A sample of each seed lot was grown in greenhouse under mist condition and in vitro using MS medium. In the latter, both intact and cut seeds were used. Germination index (containing germination velocity and rate) was used as a criterion for germination evaluation. The results showed that there was no sign of germination in intact seeds 40 days after cultivation on MS medium however, cut seeds containing plantlets started germination 2 days after cultivation and obtained 90 percent of the germination after one week of culturing on the medium. Germination of seeds under mist condition began 15 days after sowing and showed a minimum of 55 to 87 percent in different genotypes till the end of the experiment period (60 days). Germination index (GI) under this condition ranged from 15.4 to 26.1. GI comparison of seeds under in vitro and mist conditions indicates a lower germination rate in different genotypes under mist condition. This study also showed that the rate of germination in strawberry seeds could range from 0 to 100 percent depending on genotype and type of treatment used. The best treatment for a synchronized and rapid germination was found to be using cut seeds containing the plantlets on MS medium.

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Soil moisture is one of the most important determinants of plant’s seed germination, emergence and finally yield formation in arid and semi-arid zone (e.g. IRAN). In such a situation, the selection of a cultivar resistant to drought is the most important task. In this regard, a research at three levels (Farm, Greenhouse, Laboratorial) was conducted to study 10 genotypes with three improved background including: Improved Indigenous(II) genotypes (Sardary, Roushan, Omid), Improved Indigenous Foreign Crossed (IIFC)(Azadi, Falat, Qods) and four Indigenous lines (IL)(5593/2-3, 6452-6, 5806-3, 7007/2-6). On the basis of our data with decrease of osmotic potential, seed vigor with high speed and slope in different genotypes decreased. In -0.8 MPa level of osmotic potential, Omid, Azadi and 5593.2-3 genotypes had the highest and Sardary, 5806-3 and Falat genotypes had lowest seed vigor. In -1.6 MPa level of osmotic potential seed vigor of all genotypes severely decreased and in this level of osmotic potential, no significant difference was observed among genotypes. The greater correlation between seed germination, vigor and emergence percentage in farm situation was obtained but the correlation between all germination characteristics in laboratory and farm situations with yield was not significant. Orthogonal analysis shows that II genotypes have greater seed vigor in comparison with other groups of genotypes, but did not show any significat difference among the three groups. The percentage and rate of germination.

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In this investigation, the effect of seed source on the germination and survival of Acer velutinum Boiss. was assessed. Seeds were collected from 11 seed sources ( located at 20 to 2200 meter a.s.l in Mazandaran forests) and planted in a mountain nursery (Orimelk located at 1550 meter a.s.l) as completely randomized block design with three replications. The results show that the effect of seed source on germination and survival was statistically significant (p<0.01). Germination and survival rates were lower for the Pasands, Ashak and Deis sources, and the maximum seed germination was related to Lamzer site. Effect of seed sources on survival of seedling was significant too. The maximum and minimum survival was related to Lajim and Ashak, respectively. Regarding plan table seedling, the effect of seed source was significant. The maximum efficiency of seedlings has been found in Lajim, Lamzer, Parke Noor and Sangdeh sites, while the minimum range of efficiency was related to Ashak and Pasand2.

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This research was conducted in order to select male genotypes of pistachio with good performance. The study was performed on 10 genotypes in regard to their flowering time, pollen quantity and quality. The results indicated that flowering times of P1, P2 genotypes were simultaneous with Kallehghochi P3, P4, P5 genotypes with Ahmad Aghaii P6 and P7 genotypes with Ohadi and P8, P9, P10 genotypes with Akbari. Inflorescence weight showed big differences between them, ranging from 4.5gr for P6 to 1.66gr for P8. The amount of pollen per inflorescence also showed big difference, ranging from 71mg for P2 and 267mg for P5. In order to determine the percentage of pollen germination, a medium consisting of sucrose, agar and boric acid was used. The percentage of pollen germination also showed big difference, ranging from 30%for P3 and P4 and 85% for P10. Stored pollen at 4°C temperature showed 30% germination after one month and 0% germination after three months. The results also indicated that the percentage of pollen germination in culture medium without boric acid was lower, compared with other treatments. The viability of pollens collected from cutting inflorescences kept in water bottle was higher than that obtained from dried inflorescences maintained in room condition.

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In order to study the effects of salinity on germination, vegetative growth and some physiological characteristics of canola cultivars, two experiments were conducted at Research Station of Agricultural College of University of Kerman in 2004. The experimental design was a Completely Randomized Block with four replications. Treatments comprised all combinations of different levels of three factors including variety (Kobra × Regent, Ceres and Okapi), salt type (NaCl, CaCl₂) and electrical conductivity of saline solution (0, 4, 8, 12 dS/m). Results of variance analysis showed that salinity has a very significant effect on germination percentage, germination homogeneity, germination rate, rootlet and polomul length at the end of the germination stage. Shoot length, dry weight, diameter and number of nodes per shoot were significantly affected also by salinity at vegetative growth. Regarding the two types of salt, none of the measured traits were significantly different. All the mentioned traits decreased with increasing salinity the highest and the lowest values were recorded at control and at 12 dS/m salinity levels. Membrane ion leakage was the only trait which increased significantly. Comparison of means showed germination percentage and germination homogeneity at germination stage and the number of nodes at vegetative growth stage were affected less than other traits by salinity, and they only decreased significantly with increasing salinity to 12dS/m. The effect of cultivar on these traits was also significant (P<0.001). In both growth stages, cultivars response to salinity was different. At germination stage, Ceres and Kobra * Regent cultivars were the most sensitive and tolerant ones in terms of all traits and there was no significant difference between Okapi and Kobra *Regent. At vegetative growth stage, however, Okapi showed less growth than Kobra * Regent and their difference was significant. It seems that evaluation of traits response at germination stage can not be effective to determine salt tolerance of canola cultivars.

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Total petroleum hydrocarbon (TPH) contaminations in soils may be toxic to human, plants and cause groundwater contamination. To achieve maximum TpH- reduction and to establish successfull stable vegetation cover in phytoremediation method, various criteria must be considered to choose the plants carefully. In this study, germination and subsequent growth of seven plants were tested in three soils with different petroleum contamination levels. Contamination treatments consisted of C₀ (uncontaminated soil), C₁ (1:1 w/w, uncontaminated: contaminated soil) and C₂ (1:3 w/w, uncontaminated: contaminated soil). The experimental design was completely randomized split plots with three replications per treatment. The results showed that the presence of TPH in the soil had no effect on seed germination of agropyron, white clover, sunflower and safflower although canola seedlings were sensitive to these compounds and failed to produce dry matter yield (DMY) at the end of trial period. In contrast, seed germination of canola, puccenillia and tall fescue decreased in the petroleum contaminated soils. No reduction was found in DMY of puccenillia in contaminated soils (C₁ and C₂ treatments) compared to control however, the presence of TPH proportional to the contamination levels, decreased dry weight of sunflower and safflower. This reduction in growth and dry weight for tall fescue and agropyron was also observed in C₂ compared to C₁ treatment. Therefore, it seems that though agropyron, white clover, sunflower and safflower germinated well and the presence of TPHs in the soil treatments had no effect on their seed germination, they grew poorly. In contrast, grasses had poor seed germination but their subsequent growth and establishment in the contaminated soils was acceptable for subsequent phytoremediation trials.

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Soil salinity is an increasing environmental stress on crops in most areas of Iran since farmers use underground saline water for irrigation. In order to investigate the effects of salt stress on germination and seedling growth of safflower (Carthamus tinctorius L.), an experiment was conducted at two stages (germination seedling growth), using four levels of NaCl salinity (0, 5, 10, 15 ds/m), and four cultivars of spiny and non-spiny safflower ( a landrace from Isfahan bred cultivars of IL, LRV and PI). The design was factorial and completely randomized based on 3- replicates (CRD). Germination percentage, germination velocity index, root and shoot length, root and shoot dry weight, root to shoot dry weight ratio and salt tolerance index were measured at germination stage. In seedling growth stage, traits such as, stem length, number of leaves, chlorophyll index, root and shoot dry weight, root to shoot dry weight ratio and salt tolerance index were also measured. Results for both growth stages showed that salt stress decreased all the above mentioned traits, significantly, in all cultivars. Salinity × variety interaction at germination stage was also significant, compared to seedling growth stage. Salt tolerance index decreased significantly with increasing salinity at both stages, but differences between cultivars were not significant. Cultivars PI and IL were characterized as the tolerant ones at germination and seedling growth stages, respectively. It could be concluded that safflower cultivars responded differently to salinity in germination phase compared to seedling growth phase, and that safflower suffers more from salinity during germination stage.

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To compare the allelopathic potential of some Guilan province rice cultivars with allelopathically approved IRRI rice cultivars, a factorial experiment was conducted in completely randomized block design with 3 replications on the Experimental Farm of Agricultural College of Guilan University in spring 2005. Two factors including a) seven rice cultivars (traditional cultivars of Hashemi, Ali Kazemi, and Tarom and 2 modern cultivars of Khazar, and Dorfak and two IRRI cultivars of Dollar and IR64), and b) two types of planting, (including monoculture and mixed culture of each cultivar with Barnyardgrass) were used. A monoculture of Barnyardgrass was also used as a control. Growth rate (GR) and leaf area index (LAI) of Barnyardgrass were determined after 2, 4, 6, and 8 weeks of transplantation (WT), in mixed and monoculture treatments. In addition, after 3, 6, and 9 WTs the type and number of weeds in 1 m2 of each plot was determined. Rice plant samples were taken in 2, 4, 6, and 8 WTs, and were extracted in the Lab to be tested for germination for barnyardgrass and redroot pigweed seeds in Petri dishes. The filed research results showed that GR, LAI, number of weeds and yield of barnyardgrass grown with Dollar cultivar were lower than the others, which indicates higher allelopathic properties of this cultivar. Rice cultivars water extract evaluation of Petri dish also showed that Dollar cultivar has higher allelopathic potential than the other cultivars. Increasing the concentration of the water extracts increased the suppression of treated seedlings. From starting to geramination stage of the rice cultivars, allelopathic effects of the water extracts decreased. Leaves had the highest level of allelopathic potential in comparison to other organs. In other words, the pure extract of Dollar cultivar taken form samples in 2 and 6 WTs had the highest allelopathy potential among the cultivars and samples.

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Turfgrass is important in urban landscape and by considering that organic matter has a important roll in improving physical and chemical soil characteristics using an organic matter instead of manure and MSW seems necessary .in this research the possibility of using new method of culture such as hydromulching (i.e. mixing seed, organic matter, water, and fertilizer and then distributing it) spent mushroom compost (SMC) has done .for these reason we use two level of SMC 6- month old and one –year old .in two time ,spring and autumn .the experiment design as a factorial Randomized Complete Block Design with 3 replications .Germination ,establishment ,uniformity ,density ,color was measured .results showed that SMC is better than manure especially 6-month old SMC as it is than one-year old SMC .in the spring establishment and germination is better . So using hydromulching by 6-month compost in spring is recommended.

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Enzymes of wheat flour have an important role in production of various cereal products, especially bread, and therefore accurate measurement of enzymatic activity along with improvement of this characteristic is the basis of most studies in baking industry. In the present work, physical properties of three wheat varieties (Mahdavi, Kavir and M7318 that are cultivated in Isfahan province) and chemical characteristics of their flours were investigated. In order to study the changes in enzymatic activities (α-amylase, lipase and lipoxygenase), the activities of these enzymes were measured before and after germination. The relationship between root length and α-amylase activity was studied as well as the relation between lipase and lipoxygenase activities. α-amylase activity of flours was also optimized according to falling number 250 s. The effect of this optimization on the bread texture in four different times after baking, on the flour color in three different times, and on the flour acidity in two different times were then evaluated. The results of chemical measurements showed that M7318 and other two varieties can be considered as weak flours and medium flours, respectively, in terms of their protein and gluten contents. However, all varieties had low levels of α-amylase activity, although, kavir flour had more activity than the others. The results of germination tests indicated an increase in the activity of α-amylase and lipase, but not lipoxygenase. Moreover, during germination times a significant negative correlation between root length and falling number was found. Also, a significant positive correlation between root length and α-amylase activity and between activities of lipase and lipoxygenase was observed.

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Heavy metals including cadmium produced as a result of urban, industrial and agricultural activities lead to the water pollution. Also, considering the growing need for silviculture in Iran, it is necessary to conduct a comprehensive research on fast growing and evergreen species of Eucalyptus and their role in heavy metals' absorption. To achieve this goal, Eucalyptus occidentalis seedlings were grown in pots containing silica and irrigated with a mixture of nutrient solution and cadmium chloride in 5, 10 and 15 m M for 10 months. Root and leaf samples were harvested and the amount of cadmium in stem, root and leaf organs was measured. Also, some morphological and physiological characteristics were determined including soluble sugar and praline. The analysis indicated that the cadmium concentrations of 15 mM treatment in the root, leaf and stem were 585, 142 and 87 mg/kg D.W., respectively. Proline content increased with the increase of Cadmium concentration while the content of pigments decreased. The result of this research shows that eucalypt has the potential of cadmium accumulation without any serious damage to its growth. Therefore, the plant can be used to decrease the environmental pollutions.