JWSS - Journal of Water and Soil Science

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  Phytoremediation is a new technology that employs plants to remediate contaminated soils. This method compared to those that involve the use of large scale energy consuming equipments is an inexpensive method. Phytoremediation models are useful tools to further understanding the governing processes and also to manage the contaminated soils. A thorough literature review indicates that very few models have been developed for phytoremediation due to the complexity of the phenomena. The objective of this study was to develop a simple model for phytoremediation of lead and cadmium. A new formulation of phytoremediation was established based on soil and plant responses to heavy metal pollution. A large quantity of a sandy loam soil was thoroughly mixed to ensure homogeneous different concentration levels by lead and cadmium. These contaminated soils were transferred to some plastic pots. Land Cress (Barbarea verna) and Spinach (Spinacia oleracea L.) seeds were germinated in pots containing 8 kg of contaminated soil. Plants were harvested at five time intervals. The concentrations of Pb and Cd in the plant and soil samples were digested by wet oxidation and 4 M Nitric acid digestion methods, respectively, and were determined by flame and graphite furnace atomic absorption spectrometry methods. Proposed models then were calibrated using the collected data and validated quantitatively. The results indicated that the soil adsorption isotherms followed a linear form for both Pb and Cd concentrations. The results also indicated that the phytoremediation rate of Pb by Land Cress and Spinach are first-order function of Pb concentration in soil. In contrast, a zero-order function of soil Cd contaminations was obtained. Combining these two results of soil and plant responses to Pb and Cd pollution, a simple model with reasonable performance was derived to predict the time needed for remediation of soil Pb (R² > 0.98). However, in the case of Cd, the derived models appeared to be useful to make only some overall estimations of the remediation (R²≈^�0.70).

 

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  Some industrial processes, such as plating and gold mining, utilize cyanide, which entering in their effluents. Because cyanide compounds are toxic contaminants, the waste-containing cyanide must be treated before discharge in the environment. Several methods are available for cyanide removal or detoxification. Natural degradation, alkaline chlorination, and oxidation with hydrogen peroxide are the most common methods in full-scale plants. Because of technical and economical concerns related to these methods, biological treatment processes have recently come under consideration. In phytoremediation, plants potential for pollutant removal is used. The main objective of present study is to investigate feasibility and potential of phytoremediation of cyanide-polluted soils by non-woody plants. The experiments carried out in this study were a completely random factorial design procedure, with three replications. Three non-woody plants: sorghum (as a cyanogenic cereal plant) and fescues with and free of endophyte (as grasses), were examined. Analysis of variance of the data obtained on soil cyanide reduction and cyanide accumulation in plants showed that phytoremediation is a suitable technique for low concentration of cyanide-polluted soils. Besides, it was been found that sorghum has a better soil cyanide removal efficiency than fescues, so that a significant portion of soil cyanide will accumulate in sorghum tissues.

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Total petroleum hydrocarbon (TPH) contaminations in soils may be toxic to human, plants and cause groundwater contamination. To achieve maximum TpH- reduction and to establish successfull stable vegetation cover in phytoremediation method, various criteria must be considered to choose the plants carefully. In this study, germination and subsequent growth of seven plants were tested in three soils with different petroleum contamination levels. Contamination treatments consisted of C₀ (uncontaminated soil), C₁ (1:1 w/w, uncontaminated: contaminated soil) and C₂ (1:3 w/w, uncontaminated: contaminated soil). The experimental design was completely randomized split plots with three replications per treatment. The results showed that the presence of TPH in the soil had no effect on seed germination of agropyron, white clover, sunflower and safflower although canola seedlings were sensitive to these compounds and failed to produce dry matter yield (DMY) at the end of trial period. In contrast, seed germination of canola, puccenillia and tall fescue decreased in the petroleum contaminated soils. No reduction was found in DMY of puccenillia in contaminated soils (C₁ and C₂ treatments) compared to control however, the presence of TPH proportional to the contamination levels, decreased dry weight of sunflower and safflower. This reduction in growth and dry weight for tall fescue and agropyron was also observed in C₂ compared to C₁ treatment. Therefore, it seems that though agropyron, white clover, sunflower and safflower germinated well and the presence of TPHs in the soil treatments had no effect on their seed germination, they grew poorly. In contrast, grasses had poor seed germination but their subsequent growth and establishment in the contaminated soils was acceptable for subsequent phytoremediation trials.

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Phytoremediation is a new, in-situ and emerging remediation technology for contaminated soils. This technology, compared to other methods, is a sustainable, natural, relatively cheap and applicable to large scale area. Modeling phytoremediation provides quantitative insight for the governing process as well as for managers to assess the remediated sites. The objective of this study was to introduce a macroscopic phytoremediation model for Ni and Cd- polluted soils. The proposed model assumes that relative transpiration reduction function can resemble total soilNi and Cd concentrations. Combining the related functions of soil and plant responses to soil Ni and Cd concentrations, the phytoremediation rate of Ni and Cd was predicted. In order to test the proposed model, large quantities of soil were thoroughly polluted with Ni and Cd. Upland Cress (Lepidum sativum) and Ornamental Kale (Brassica olerace var. Viridis) seeds were then germinated in the contaminated soils. The experimental pots were irrigated with fresh water to reach field capacity. Upland Cress and Ornamental Kale were harvested three and four times, respectively. At each harvest, relative transpiration, Ni and Cd contents of soil samples and plants were measured. Comparison of the maximum error, root mean square error, coefficient of determination, modeling efficiency and coefficient of residual mass indicated that the non-threshold non-linear model provide high efficiency to predict relative transpiration for Upland Cress and Ornamental Kale, respectively. The results also indicated that the proposed macroscopic model can well predict the phytoemediation rate of the Ni and Cd by Upland Cress (R2>0.83) and Ni by Ornamental Kale (R2=0.78).

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Presence of petroleum contaminants in soil may be toxic to human and organisms and act as a source of ground water contamination hence, remediation of these compounds from environment is vital. In this study, first the feasibility of remediation of two petroleum-contaminated soil samples around Tehran Oil Refinery (oil refinery landfill and agricultural soils) was assessed using landfarming technique during a four month experimental period. The elimination of total petroleum hydrocarbons (TPHs) from soils treated through landfarming technique was then investigated in the rhizosphere of agropyron and fescue. The results showed that microbial respiration increased due to landfarming processes in both soils. Urease activity in the landfarming treatment for agricultural soil was 21, 45, 26, and 23% higher than the control at the end of first to the 4th months of experiment, respectively. However, no significant differences were observed between the landfarming and control treatments for landfill soil at the end of experiment. Furthermore, about 50 and 57% reduction in TPH-concentration was observed in the landfarming treatment for landfill and agricultural soils at the end of experiment, respectively. In the phytoremediation study, presence of TPHs in both landfarming and control treatments reduced dry matter yield of the studied plants. Urease activity in the rhizosphere of fescue and agropyron was higher than in the unplanted soil. Degradation of petroleum-compounds in the landfill soil under landfarming treatment was more than 20 and 40% in the presence of fescue and agropyron, respectively. The influence of agropyron on TPH-removal from agricultural soil under the landfarming treatment was also higher than fescue.

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In this study, the effects of enhancing synthetic chelators (HEDTA, EGTA) and low molecular weight organic acids (LMWOA) such as citric acid were compared on cadmium (Cd) solution in soils that were artificially contaminated. Also Cd phytoextraction capability by radish (Raphanus Sativus L.) was studied. The experiment was laid out in a randomized complete factorial design where each treatment was replicated three times. Concentration treatments of cadmium using CdCl2 were 0(control), 5, 20, 60 and 100 mg Cd kg-1. After complete growth of plants, 6, 20 and 20 mMkg-1 soil HEDTA, EGTA and Citric Acid were added per pot, keeping a control without any chelator application. In order to determine cadmium concentration ten days after adding chelates, samples were taken from the plants and soil of pots. The results showed that in all treatments the concentration of soluble Cd in soil was higher than the control. Also the results showed that synthetic chelators as compared with LMWOA (Citric Acid) have increased the solution remarkably. Among the other chelates, HEDTA had significant effects on Cd solution. In the current study, Cd concentration in shoot and root of (Raphanus Sativus L.) was increased with enhancement of Cd concentration in soil. Cd concentrations in shoots of radish were higher than those in roots. This could refer to higher bioavailability and solubility of Cd. In the current study, in all the treatments with HEDTA Cd concentrations in shoot and root of (Raphanus Sativus L.) were increased as compared with other chelates..

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A greenhouse experiment was conducted in factorial experiment arranged as a completely randomized design (CRD) to evaluate the role of tree indigenous arbuscular mycorrhizal fungi (AMF) species originated from a polluted soil in phytoremediation of zinc polluted soils using maize as a host plant. The experiment consisted of plants inoculated with AMF (G1(Glomus intraradices), G2(Glomus mosseae) and G3(Glomus versiforme)) and G0 as non-inoculated plants and 5 levels of zinc (0, 10, 50, 100 and 500 mg kg -1) in non-sterilized sandy loam soil with three replications. According to the results of greenhouse experiment, the zinc and phosphorus uptake and also the biological yield of maize plants were significantly increased by inoculation with AMF in comparison with non-inoculated plants and also no zinc toxicity symptoms were observed. Uptake, translocation, and phytoextraction efficiency of plants inoculated with G. intraradices was more than the other treatments up to the level of 100 mg kg -1, but at the level of 50 mg kg -1 these amounts were highest in plants inoculated with G. mosseae. The efficicncy of three AMF in zinc uptake was highest at the low level of zinc. In general, under the high soil pollution (500 mg kg-1), G. mosseae was the most effective fungal species in Zn extraction and translocation while G. intraradices had the highest effectiveness for accumulation of Zn in the roots. The overall situation of G. versiforme was mostly between the two other fungal species.

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The petroleum refinery sludge is an important source of environmental pollution. Burning and burying of the sludge may have adverse effects on environment and human health. Thus, other mechanisms for decreasing the toxic effects of hydrocarbon substances in the sludge must be used. In this study, Isfahan refinery sludge was dewatered, air dried and mixed by 0, 10, 20, 30 and 40% w/w ratio with two calcareous soils, viz., Mahmoud Abad (Typic Haplocalcids with clay texture) and Bagh Parandegan (Anthropic Torrifluvents with silty loam texture). Different mixtures of soil and sludge were farmed for 21 days and irrigated on a daily basis to field capacity. Then, 100 seeds of Tallfescue (Festuca arundinacea) and Agropyron were planted in polluted soils with 3 replicates in 3 kg pots for 5 months. Result showed that Tallfescue and Agropyron yields decreased in sludge contaminated treatments. In the 40% sludge treatment, Tallfescue decreased the total petroleum hydrocarbons content by 65 percent. The highest degradation for agropyron was in the 30% sludge treatment which showed about 55% reduction in total petroleum hydrocarbons. The 40% sludge treatment resulted in the minimum yields of root and shoot plants. The highest degradation of TPHs occurred in the Tallfescue rhizospher of 40% sludge. Maximum degradation of TPHs on the Agropyron rhizospher was in 30% sludge mixed with Bage parandegan soil, but maximum yield of plant was in 20% sludge. Our study shows that Tallfescue rhizospher is most effective for decreasing TPHs, and that the phytoremediation in soils with more clay can adsorb and fix the toxic components and then at higher levels of pollutions can let the plants grow.

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Arbuscular mycorrhizae (AM) and Plant Growth Promoting Rhizobacteria (PGPR) associations are integral and functioning parts of plant roots. These associations have a basic role in root uptake efficiency as well as improvement of plant growth in degraded environments including heavy metals contaminated soils. This study was conducted to evaluate the effects of heavy metal-resistant soil microbe's inoculation on bio-availability of Pb and Cd in soil, plant growth as well as metal uptake by Millet (Pennisetum glaucum), Couch grass (Triticum repens) and wild alfalfa (Medicago sativa). A soil sample was treated by different levels of Pb and Cd (soil 1). Native microbial inoculums were obtained from alfalfa rhizosphere soils adjacent to Pb and Cd mines in Zanjan region (soil 2), then added with weight ratio of 1:5 (w/w) to soil 1. Host plants including millet, couch grass, and alfalfa were grown in pots and kept in greenhouse conditions. At the end of growing period, shoot dry matter and Pb and Cd concentrations in plant and soil were measured. Results indicated that plants yield and Pb uptake were significantly higher in non-inoculated treatments (p ≤ 0.05). However, Cd uptake by plants was greater in inoculated treatments (p ≤ 0.05). Couch grass showed the most accumulation potential of Cd and Pb among the studied plants.

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The reclamation of salt-affected soils which occur on 831×106 ha can be effective in increasing agricultural production. Cultivation of plant species which are resistant to salinity can improve the soil by increasing the solubility of calcite and releasing the calcium in soil solution. This study was conducted as a column experiment with a saline-sodic soil (SAR = 23.8, EC= 12.88 dS m-1, pH= 7.7, CaCO3= 15.15 %). Three plant treatments including Sesbania acuelata, Cyanodon dactylon and Rubia tinctorum, and three chemical treatments including gypsum in two levels (50% and 100% gypsum requirement) and sulfuric acid with a control were arranged. All treatments were replicated 3 times. The soil columns were similarly leached by 41 liters of tap water during 30 days in 8 stages. After leaching, SAR and EC in soil, the amounts of sodium in leachate and total amount of sodium in plants shoot were determined. Results showed that the SAR was decreased compared to control by the plant treatments and the chemical amendments by about 59% and 65%, respectively. Moreover, two plants of Cyanodon dactylon and Rubia tinctorum had maximum amount of leachate sodium, which shows an impressive role of these plants in dissolution and leaching of exchangeable or sediment sodium in comparison with the other treatments. According to salient performance of phytoremediation in improvement of physicochemical properties of soil compared to chemical amendments, phytoremediation can be recommended as a profitable low-cost and effective method for remediation of saline-sodic soils.

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Phytoremediation models are important to understand the processes governing phytoremediation and the management of contaminated soils. Little effort has been made for evaluating the potential of the phytoremediation of metals based on the mathematical models. Therefore, the purpose of this study was modeling the phytoremediation of the nickel-contaminated soils. For this purpose, a model was recommended for estimating the rate of the phytoremediation of nickel from the soil by means of relative transpiration reduction and concentration of nickel in the plant functions. To evaluate the model, soil was contaminated with different levels of nickel by nickel nitrate. Then, the pots were filled with contaminated soil and Basil (ocimum tenuiflirum L.) seeds were planted. To avoid the dry tension, the pots were weighed and irrigated to the point of field capacity (FC) at short time intervals (48 hours). The plants were harvested in four times. At each harvesting stage, the relative transpiration values and nickel concentration in the soil and plant samples were measured. The performance of the model was evaluated by statistical methods such as Maximum Error, Root Mean Square Error, Coefficient of Determination, Efficiency of Model and Coefficient of Residual Mass. Results demonstrated that in the case of nickel contamination in soil, changes in the relative transpiration of Basil can be measured by the two proposed models and the linear model (R2=0.94) has a better performance compared to the nonlinear one (R2=0.84). Also the model obtained from the combination of linear function and nickel's concentration in soil has a relatively good (R=0.7) fit with the measured values of the remediation rate of nickel in soil.

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Phytoremediation is one of the best methods for cleaning heavy metal contaminated soils. In this method, tolerant plants with high absorption potential are used to clean up the pollutant. The purpose of this greenhouse study was to find the most suitable plants with the highest Zn translocation factor and shoot concentration. Soil samples were collected from zinc and lead Bama mine in Isfahan province, Iran. To compare the hytoextraction power, Heliantus annus.L., Thlaspi caerulescens, Trifolium pretense L. and Amaranthus retroflexus were planted in 8 kilogeram pots. The experiment was conducted in completely randomized design with three replications. After seventy days, plants root and shoot were harvested and Zn concentration was measured. The result showed that Thlaspi caerulescens had significantly higher root and shoot Zn concentration (3125 and 4041 mg kg-1, respectively). The highest bioaccumulation factor and translocation factor was observed in Thlaspi caerulescens. Based on this research, Thlaspi caerulescens was one of the suitable Zn hyper accumulators for cleaning up the Zn pollution.

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Phytoremediation is one of the cleanup methods of polluted soil that is possible accumulation of heavy metals in plant tissues, exclusion of these elements from contaminated soil. Therefore, to achievement the objective, this research was done in pot culture using completely randomized design at the University of Birjand in 2011. Two species Spinacia oleracea and Lepidium sativum were used to remove or reduce the concentration of Cadmium (Cd) and Chromium (Cr). In this study, different levels of Cadmium (CdCl2) concentrations including 5, 50, 100 mg kg-1 and also chromium (CrCl3) concentrations 50, 100, 150 mg kg-1 were used respectively and control as well for each species with three replications. Results indicated that the Cd and Cr concentration in shoot of Spinacia oleracea and Lepidium sativum significantly affected by their concentration in soil (p<0/01). Results revealed that increasing of Cd and Cr concentrations in soil, showed an increase concentration of both metal in shoot of Spinacia oleracea. increasing of Cd concentrations in soil, showed an increase concentration of it in shoot of Lepidium sativum but the concentration of Cr was less. Also, comparison of cadmium and chromium concentrations in shoot of Spinacia oleracea and Lepidium sativum showed that two species showed same behavior of Cd and different behavior Cr concentration. So the analysis of data showed that both of species are appropriate for absorption of Cd and Cr and phytoremediation technology as well. It can be concluded that in high soil Cr concentration for phytoremediation Lepidium sativum is not appropriate.

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Cadmium is a trace element which is harmful to life and is considered as a dangerous pollutant. This element leads to pollution and reduction of water quality and sometimes even to toxicity through contaminated sources such as wastewater (municipal and industrial). Due to the growing population's need for more water resources and increased water resource pollution, a need for new and inexpensive methods for remediation and improving water quality is felt. Phytoremediation with aquatic macrophytes is an effective and inexpensive method for improving water quality and wastewater. In this study, biological removal of cadmium from simulated wastewater was reviewed within 11 days of cultivation of Lemna gibba in Hoagland nutrient solution, at four different concentrations of cadmium (0, 1, 2, 4, and 6 mg L-1). Maximum Bioconcentration Factor and maximum Uptake Index were calculated from 6 mg L-1 metal concentration. Maximum (4.71 g/day) and minimum (2 g/day) Biomass production measurement was obtained from 0 mg L-1 and 6 mg L-1 of pollutant concentration. The plant used in this study was able to accumulated cadmium with the efficiency of up to 91%. However, the pollutant remediation was not completed in a short time. Thus, pollutants' bioremediation from wastewater solutions by Lemna gibba, a native hydrophyte of southern Iran’s pounds, is efficient and appropriate.

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Cadmium (Cd) is a metal with high toxicity and solubility in water, which is a serious environmental threat to human health. Phytoremediation is an environment-friendly method and a promising new and cost effective technology that uses plants to clean organic and inorganic contaminated media. This study was conducted to evaluate the potential of Jatropha curcas for remediation of soils contaminated with Cd. Seedlings were planted in the soil spiked with Cd in amounts of 0, 25, 50, 75, 100 and 150 mg kg^-1 (Cd₀, Cd₂₅, Cd₅₀, Cd₇₅, Cd₁₀₀ and Cd₁₅₀) for a period of five months. Biocentration factor (BCF, metal concentration ratio of plant roots to soil), translocation factor (TF, metal concentration ratio of plant shoots to soil) and removal efficiency (RE, total metal removed by plant biomass to total metal loaded in soil) were determined. Cd concentrations among plant parts were in the following trend: roots>stems>leaves. The highest total Cd concentration (up to 1100 mg kg^-1) and the highest RE were found in Cd₁₅₀ and Cd₂₅, respectively. BCF and TF of the plant were more and less than 1, respectively. Hence, although this species has a potential to be used in phytostabilization of Cd-contaminated soil, more researches in the field condition are needed.

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