Showing 5 results for Somatic Embryo
R. Fotouhi Ghazvini, S. Shirani,
Volume 6, Issue 2 (7-2002)
Abstract
The effect of different media including the growth regulators on somatic embryogenesis from Mexican lime was studied. The average embryo formation after 60 days from unfertilized ovules in different media was evaluated at 0 to 33.75%. The most effective hormone to stimulate embryogenesis was GA3 at 0.01 and 0.1 mg/l concentrations. Malt extract (ME) at 300 mg/l increased embryo formation, whereas at 1000 mg/l, it inhibited embryogenesis but produced embryogenic callus. Banzyl adenine (BA) at 0.1 and 0.01 mg/l concentrations had significant effects on embryogenesis, while embryo formation was inhibited at high concentrations. Embryo development in different media was also studied. The index of mean root length to mean stem length in the MT3 medium supplemented with 0.1 mg/l of GA3 was observed to be 1.22±0.22, which was regarded a suitable medium for embryo formation, embryo development and plantlet regeneration.
A. Deljou, O. Karami, M. Esna-Ashari,
Volume 11, Issue 1 (4-2007)
Abstract
In vitro regeneration of four cultivars of carnation namely ‘Nelson’, ‘Impulse’, ‘Sagres’ and ‘Spitit’ through somatic embryogenesis was studied. MS culture medium was supplemented with 30 gl-1 sucrose, 2 mgl-1 2,4-D and 0.2 mgl-1 BA and used for embryogenic callus formation. Somatic embryos were formed when embryogenic callus was transferred to MS medium without growth regulators containing 30 g/l-1 sucrose alone or supplemented with different concentrations of mannitol (15, 30, 60, 90, 120 and 150 gl-1). No somatic embryo was formed on culture media containing mannitol without sucrose. Number of somatic embryos produced from embryogenic calli significantly increased by adding mannitol to the culture media. Normal embryos formed on culture media containing high concentrations of mannitol (60, 90, 120 and 150 gl-1) developed normally. About 95% of somatic embryos transferred to the1/2 MS culture medium containing 30 gl-1 sucrose, germinated into plantlets. Plantlets also continued their growth under greenhouse conditions.
O. Karami, A. Deljou, A. Mahmoudi Pour,
Volume 12, Issue 43 (4-2008)
Abstract
In vitro regeneration of two cultivars of carnation, namely, ‘Nelson’ and ‘Impulse’ was studied through direct somatic embryogenesis. Somatic embryos were formed directly on petal explants cultured on Murashige and Skoog (MS) medium containing different concentrations (0.2, 0.5, 1, 2, 4, 6 mg 1-1) of picloram. Maximum embryogenesis was obtained with 1 and 2 mg/l picloram. Globular shaped embryos were developed into cotyledonary-shaped embryos when they were transferred to the growth regulator-free media containing different concentrations (2, 4, 6, 8, and 10 %) of sucrose. Increasing sucrose concentrations in the culture media enhanced somatic embryos development. Cotyledonary somatic embryos developed plantlets when they were transferred to the half-strength MS culture medium containing 3% sucrose. Plantlets also continued to grow under greenhouse conditions.
A Habashi, A Mousavi, M Kaviani, S Khoshkam, A Rostami,
Volume 12, Issue 46 (1-2009)
Abstract
Date palm (Phoenix dactylifera L.) is propagated traditionally through offshoots or suckers, which usually appear at or below the ground level surrounding the stem base. However, there are many problems associated with this system. Offshoots are produced in limited number and vegetative propagation through them is slow, laborious, time consuming and expensive. The present study was conducted to determine the best micropropagation protocol for date palm in Kebab, Estameran, Piarom, and Berehi cultivars. The shoot apical meristem from two to three-year-old offshoots was used as source of explants. They were cultured in callus initiation medium, containing different concentrations of 2,4-D (40, 60, 80 and 100 mgl-1), NAA (10 and 20 mgl-1) and 2ip (3 and 5 mgl-1). All cultivars produced high percentage of callus with good quality in a matter of callus friability and color in 100 mgL-1 2,4-D, 20 mgL-1 NAA, and 3 mgL-1 2ip. Kabkab cultivar was superior for callus production (87.25%) in comparison with other cultivars. The calli were then transferred to a proliferation medium and then transferred to somatic embryogenesis medium containing different concentrations of Kinetin (2, 4 and 6 mgL-1), BAP (2, 4 and 6 mgL-1), and NAA (0.1, 0.5 and 1 mgL-1). Somatic embryogenesis was observed in MS medium supplemented with 2 mgL-1 kinetin, 2 mgL-1 BAP, and 0.1 mgL-1 NAA. Kabkab and Estameran cultivars showed higher somatic embryogenesis in comparison with other two cultivars. The somatic embryos were then transferred to MS medium without hormones under light, where they produced shoots and roots. Abbriviations: 2,4-D- 2,4-dichlorophenoxiaceticacid 2ip-N6(2-isopentenyl)adenine NAA-Naphthalene acetic Acid BAP-6-Benzylaminopurine MS-Murashige and skoog (1962).
M Ebrahimi, S.m Khayam Nekoei, S Kadkhodaei,
Volume 12, Issue 46 (1-2009)
Abstract
Somatic embryogenesis is affected by several factors. In this research project, we studied the effect of explant size, wounding and desiccation treatments on somatic embryogenesis and their conversion into plantlet among three genotypes of soybean. The explants were sampled from immature embryos of soybean in three different sizes (3, 5 & 7 mm) with wounding treatment on half of each, and then were cultured on the somatic embryogenesis medium. In order to determine desiccation effect on conversion amount of embryos into plantlets, the produced embryos were affected by three levels of desiccation treatments (2, 4 & 6 days). The increase ratio of callus mean weight, percentage of embryogenic calli, embryo number per explant and percentage of embryo conversion to plantlet were used for treatment evaluation. Variance analysis of the data showed significant differences (P<0.01) between treatments regarding the variables. The results indicated that BP was a superior genotype with embryogenic capability (24.19 %) and the best explant size for somatic embryogenesis was immature embryo with 3 mm length. The six day desiccation treatment caused highest percentage of embryo conversion into plantlet (74.7 %). Wounding increased callus production on explants and number of embryos per explant (20.28), but it did not show any significant effect on percentage of embryogenic calli. Germinated somatic embryos were transferred to pots containing peat-moss. Somatic embryogenesis is an efficient method for the plant regeneration and genetic transformation. However, this method still offers low percentages of plant regeneration, and is perhaps related to the maturation process and high morphological abnormalities of the matured embryos. This study aimed to find some solutions for soybean somatic embryogenesis problems.
