JWSS - Journal of Water and Soil Science

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An experiment was conducted to determine growth and carcass characteristics of male broilers subjected to early feed restriction. Restricted birds were fed equivalent to 1/5*BW^0.67 Kcal/day/bird between days 7 and 14. On days 0 to 7 and 14 to 56, all chickens received feed ad-libitum. Diets of feed-restricted birds were supplemented with 15% higher methionin and cystine recommended by NRC (1994) during refeeding in starter and grower periods. No significant differences were observed for liver, breast, heart, carcass and liver fat between early restricted birds and ad-libitum control at 56 days of age. Body weight for restricted broilers were significantly (P<0.05) lower than that of controls in 56 days. However, restricted broilers had significantly lower total-feed to gain ratio than the unrestricted control. Restricted broilers that received diets high in total sulfur amino acids in starter and grower periods had singnificantly lower abdominal fat pad than control birds.

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Digestible lysine and sulfur amino acid (SAA) requirements of Ross male broilers were determined during the starter period in two experiments. Broilers were fed a control corn-soy diet (3200 kcal MEn/kg and 23% CP) for 6 days and then randomly assigned to dietary treatments until 16 days of age. The basal diet containing 19% CP was prepared in order to be deficient in lysine for experiment 1 and SAA for experiment 2. Seven graded increment levels of L-lysine HCl or Dl-methionine were added in absences of starch in experiment 1 and 2, respectively. All diets contained 3200 kcal MEn/kg and all essential amino acids, except for the test amino acid, were 10% higher than the NRC (1991) recommendations. The digestible lysine requirements of broilers based on broken-line and exponential models were 1.04 and 1.01%, respectively. The digestible SAA requirements for the mentioned models were 0.846 and 0.841%, respectively.

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This study was conducted to evaluate the effects of different levels of energy and protein on the performance of male broiler breeder chicks. Three hundred and sixty day-old Arian male broiler breeder chicks were used in a 2×3 factorial experiment with two levels of metabolizable energy (3050 and 3200 Kcal/Kg) and three levels of protein and sulfur amino acids (110% NRC, NRC and 90% NRC) in a completely randomized design with four replicates per treatment. Results obtained from the whole of the experiment’s period (0-49 days of age) indicated that level of dietary energy had a significant effect on body weight and daily gain (P<0.05). Increasing of the energy levels from 3050 to 3200 kcal/kg increased body weight (2216g vs 2314g) and daily gain (45.2 vs. 47.2 g per day). Dietary energy level had no effect on feed intake, feed conversion and carcass components. Increasing of dietary protein significantly increased body weight and daily gain and decreased feed conversion and percentage of abdominal fat pad (P<0.05). The results of this study indicated that we can fed Arian male broiler breeder chicks by using diets containing 3200 Kcal ME/kg and protein and sulfur amino acid levels according to NRC recommendation or 10% higher during 0-21, 21-42 and 42-49 days of age and achieve suitable growth rate and body weight.

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In the current experiment seven catheterized broiler breeder hens were used at 60 weeks of age. Retention of ¹⁴CO₂ in the body of the hens following continuous infusion of NaH¹⁴CO₃ during each of tow-30-hr continuous infusion studies was determined. Oxidation rate plateau was established within two hours in each experiment. Recovery of ¹⁴CO₂ at the steady state in the first and second study were 91.09 and 87.55 percent respectively and were not significantly different. Mean breath ¹⁴CO₂ recovery was 89.32 percent and hence retention was 10.68 percent. Recovery of ¹⁴CO₂ in breath was not significantly affected by laying of egg or photoperiod. Recovery of ¹⁴CO₂ during the fed state was significantly lower than the fasted state. The results of this experiment indicated that, using of uncorrected data for the retained isotope into body, lead to 10.68 percent AA requirements underestimation in broiler breeder hens by indicator amino acid oxidation technique.

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A chick bioassay with chemically defined amino acid (AA) diets was conducted to compare four different AA profiles: the NRC 1994, Feedstuff 2002, Rhone Poulenc Animal Nutrition 1993(RPAN) and Illinois Ideal Chick Protein (IICP) AA profiles. This battery study involved male and female chicks during 7 to 21 days of age. Indispensable AA were rationed to lysine according to requirement ratios presented in the four profiles. Digestible lysine set at 1.07 and 0.98 % of diet for male and female respectively. This experiment was carried out in a completely randomized block design using 2 5 factorial arrangement with for replicate per each diets were kept isonitrogenous (2.6 % N ) by varying levels of L-glutamic acid. All diets were checked to have at least 0.3 % proline and 0.6 % glycine. Diets for all profiles contained 3200 kcal ME/kg and a positive control diet were used according to NRC 1994 recommendations. Chicks fed a common corn-soybean meal diet for 160 h and were raised in the floor pen. Then chicks weighted individually and allocated to battery pens so that most uniformity between pens occurs. Four battery pens of five chicks were fed one of four different profiles or positive control diet in both male & female. Weight gain and feed efficiency measured for each pen at day 21. Results indicate an improved weight gain and feed efficiency in male rather than female (p<0.5). Chicks fed positive control diet weighted more (p<0.5). Between semi purified diets chicks fed diets formulated with NRC AA ideal ratios had significantly (P<0.5) better weight gain and gain: feed in both sexes relative to IICP and RPAN, but not to Feedstuff. RPAN had worst weight gain and feed: gain in females. Results of this experiment indicated that new ideal ratio of theronine (relative to lysine) in IICP for starter period may be under-estimated.

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In order to assess and determine amino acids profile and their availability in different sorghum grain (SG) varieties, three SG varieties including low tannin (0.09% , LTS), medium tannin (0.19%, MTS) and high tannin (0.37%, HTS) were grown at the same location. The grains were analysed for their proximate analyses, tannin and amino acids. True amino acid availability (TAAA) was obtained by Sibbald’s method using ceacectomized single comb leghorn cockerels. The results showed that methionine and cystine were the first and the second limiting amino acids, respectively in LTS and MTS. But in HTS, lysine (0.166%) was the first and methionine (0.176%) was the second limiting amino acid. Methionine content was 0.114% , 0.182% and 0.176% for LTS, MTS and HTS, respectively. Although, crude protein was lower in HTS (10%) than the other SG, but histidine, methionine and isoleucine were higher for HTS in comparison to LTS and MTS. TAAA decreased when tannin content increased and all amino acids had a lower availability in HTS than LTS and MTS (P<0.05). Methionine in HTS and LTS and glutamic acid in MTS had the highest availability. The availability of proline was most affected by tannin, and its availability was 91.55% for LTS, 84.82% for MTS and 22.82% for HTS (P<0.05).