M. Falahati-Anbaran, A. A. Habashi, M. Esfahany, S. A. Mohammadi, B. Ghareyazie,
Volume 10, Issue 3 (10-2006)
Abstract
Annual medics are used for hey production, soil protection, biological fixation of N2 and green manure. In the present study, the inter and intra specific genetic diversity and relatedness of 4 diploid and two tetraploid (M. rugosa and M. scutellata) annual medics were evaluated using microsatellite markers. PCR analysis was performed on genomic DNA from individual plant and PCR products were detected using standard polyacrylamide sequencing gel. Totally twenty five polymorphic alleles were observed in the studied species. Average intra-specific genetic diversity ranged from zero (0.0) in both M. rugosa and M. scutellata to 0.114 in M. minima species, and the level of genetic diversity was similar in both M. orbicularis and M. truncatula species. Analysis of molecular variance (AMOVA) was used to partition the overall genetic diversity into within and among species, and between diploids and tetraploids. The results revealed significant (P<0.05) inter and intra-specific genetic variation. Pairwise comparisons based on Fst indicated significant differences among all of the species. Clustering analysis using UPGMA algorithm based on coancestary coefficient revealed a clear genetic relationship among species. The hypothesis on a common origin of two tetraploid species was supported by UPGMA clustering and phylogenetic analysis. The high level of Genetic diversity in spiny pod species respect to spineless pod species suggested the high importance of species with spiny pods in annual medics evolution. The findings support the usefulness of microsatellite markers for assessing inter and intra specific genetic diversity, differentiation and genetic relationships.
N. Hedaiat, Y. Rouzbehan, S. A. M. Modares Sanavi,
Volume 11, Issue 41 (10-2007)
Abstract
The nutritive value of the annual alfalfa (Medicago rigidulla, Medicago polymorpha and Medicago scutellata) species harvested at flowering stage was assessed by chemical composition, in vitro dry matter and nitrogen digestibility, in sacco dry matter and nitrogen degradation (0, 8, 12, 24, 48 and 72 h) and palatability (short-term intake rate, STIR) methods. Mean values of the chemical analysis results (%) for M. rigidulla, M. polymorpha and M. scutellata respectively were as follows: OM 85.1, 86.1 and 86.9 CP 25.1, 23.8 and 15.6 NDF 23.2, 24.8 and 30.0 ADF 18.3, 19.9 and 24.0 ADIN 0.36, 0.11 and 0.22 calcium 1.4, 1.3 and 1.2 phosphorus 0.23, 0.28 and 0.24 potassium 1.5, 1.5 and 1.4. The digestibilities of the DM and OM for M. rigidulla were 0.82 and 0.79 M. polymorpha 0.83.5 and 0.80 M. scutellata 0.75 and 0.69, respectively. The degradabilities of DM and CP at outflow rate of 0.05 for M. rigidulla were 0.72 and 0.55 M. polymorpha 0.71 and 0.57 M. scutellata 0.63 and 0.58, respectively. Finally, the palatabilities (using short-term intake rate method) for M. rigidulla, M. polymorpha and M. scutellata species were 13.6, 12.8 and 11.3 (g DM/min) respectively. According to the methods used, the descending ranking order (high to low) of these species on the basis of their nutritional value was M.rigidulla, M.polymorpha and M.scutellata.
