Showing 2 results for Antioxidant Enzymes
Z Amini, R Hadad, F Moradi,
Volume 12, Issue 46 (1-2009)
Abstract
The effects of irrigation, dry farming and drought treatments on the activities of antioxidant enzymes including superoxide dismutase, ascorbate peroxidase, catalase and peroxidase in barley leaves at different generative growth stages under field conditions were investigated. Three senescence parameters including chlorophyll, total soluble protein and rubisco large subunit protein loss, were also studied in order to compare our results to those reported by other researchers. The results showed that leaf relative water content (RWC), chlorophyll and total soluble protein and rubisco large subunit protein content declined with leaf age and the effect of water deficit. The activity of superoxide dismutase declined with the progress of the leaf age on all treatments but ascorbate peroxidase activity declined with leaf age only in irrigated (control) plants. There were no significant differences among developmental stages in catalase activity in control plants, while catalase activity declined in the water dry farming and drought stress conditions. Peroxidase activity increased with the progress of senescence for all of treatments in such conditions. Water deficit stress triggered increases in antioxidant enzymes activities. Results showed that among all studied enzymes, peroxidase has a key role in increasing resistance to oxidative stress on both the senescence stages and drought stress condition in Hordeum vulgare.
A Dolatabadian, A Modares Sanavi, M Sharifi,
Volume 13, Issue 47 (4-2009)
Abstract
In order to study the effects of salt stress and leaf feeding by ascorbic acid on antioxidant enzyme activity, proline accumulation and lipid peroxidation on leaves and roots of Brassica napus L. cvs. Okapi, an experiment was conducted as a factorial in a completely random design with three replications. Plants were exposed to salt stresses by NaCl solution (200 mM) and foliar were treated by ascorbic acid solution (25mM). Catalase, Superoxide dismutase and peroxidase activity were assayed in shoot and root. Also, malondialdehyde content and proline and chlorophyll in leaves were determined. The results showed that the activity of all antioxidant enzymes (except for SOD in roots) were increased significantly in plants under salt stress, and ascorbic acid application, as a antioxidant decreased their activity in leaves, but in roots it was not effective. Total protein content in leaves and roots decreased significantly under salinity condition. Ascorbic acid treatment increased total protein content under salt stress in both roots and shoots. Measurement of malondialdehyde content in leaves and roots showed that lipid peroxidation was increased by active oxygen species due to salt stress, and ascorbic acid reduced lipid peroxidation only in leaves. Chlorophyll content was also decreased by salinity compared to control. According to the result of present study, ascorbic acid application decreases deleterious effect of salinity.
