Showing 5 results for Bun
R. Amiri, A. Rezai, M. Shahedi, S. Dokhani,
Volume 3, Issue 3 (10-1999)
Abstract
This study was conducted to evaluate the capability of reversed-phase high performance liquid chromatography (RP-HPLC) of wheat (Triticum aestivum L.) storage proteins, and their genetic variability in different winter and spring isolines, cultivars and landraces. Gliadin proteins were extracted from the flour of five randomly selected seeds of each genotype. In this method, Nucleosil C18 300 A column (250×4.6 mm ID), equipped with a guard column and acetonitrile containing TFA as mobile phase was used.
All selected conditions for RP-HPLC such as suitable velocity and resolution were sufficient to achieve the objectives of this study. Under these conditions, the number of gliadin components by RP-HPLC analysis was more than electrophoresis subunits. In addition, quantitative results of RP-HPLC facilitated the data analysis. Also gliadin analysis by RP-HPLC had a high potential in detecting rye (Secale cereale L.) genes, so that the presence of ω-secalins in the genome of Falat variety was easily detected. Therefore, it was concluded that RP-HPLC analysis of gliadin proteins is as efficient as electrophoresis, and could even replace it in some cases. The result of cluster analysis for gliadin polypeptides indicated the gradual increase of genetic variation from isolines to landraces. Generally speaking, among the landraces, Ali Abad, Aghda, Sefid Bafghi, Ghermez Bafghi, Shahdas and Sorkheh had greater genetic variations.
E. Rowghani Haghighi Fard, M. J. Zamiri,
Volume 6, Issue 4 (1-2003)
Abstract
The effect of adding two urea levels (0.5 and 0.75% on fresh basis) to whole-crop barley (WCB) on chemical compositon and digestibility in Ghezel rams was studied. The effect of including these silages in the ration of fattening Ghezel and Mehraban rams was also investigated.
Urea supplementation of WCB silage resulted in an increase in pH, total nitrogen level, and ammonia-nitrogen concentrations (P<0.05). Apparent digestibility coefficients of dry matter, organic matter, acid detergent fiber, and neutral detergent fiber of WCB silage were not affected by urea treatment (P>0.05), but apparent digestibility coefficient of total nitrogen was higher for 0.5% urea-WCB silage. Apparent digestibility coefficients of total nitrogen and nitrogen balance were higher for 0.5% urea-WCB silage than for 0.75% urea-WCB silage, but the differences were not significant. Ruminal fluid pH at 2.5 h, and ammonia-nitrogen concentration at 4 h post-feeding were higher for 0.75% urea-WCB silage than for 0.5% urea-WCB silage (P<0.05). BUN levels (<20 mg per dl) in sheep fed with a mixture of alfalfa hay and silages were not affected by the treatment. The potential and effective degradabilities of dry matter and crude protein were higher for 0.75% urea-WCB silage. Feed performance and carcass characteristics were not significantly different amongst diets. The results showed that urea supplementation at 0.75% fresh basis, at the time of ensiling, may have a beneficial effect on feeding value of WCB silage.
M. Valizadeh, A. Safarnejad, G.h. Nematzadeh, S.k. Kazemitabar,
Volume 11, Issue 42 (1-2008)
Abstract
Parsi Zira, Bunium persicum (Boiss.) B. Fedtsch., which is called Mountainous or Black Zira, is one of the most important medicinal plants with high economic importance. Generally, there is a little information about in vitro culture of Bunium persicum. Fragmented embryo was used as an explant in Bunium persicum regeneration. In this method, a great callus induction and regeneration only on the same medium without any subculture occurred because of being young and having better interaction with medium, leading to reduction of tissue culture time, infection and chemical consumption. In this research, B5 media containing different concentrations of plant growth regulators, NAA and 2,4-D only or together with Kin, were used. The experiment was conducted using completely randomized design with 30 treatments and 10 replications per treatment. The highest callus number was obtained from the treatment containing 0.1 mg/l 2,4-D and 2 mg/l Kin or 1 mg/l NAA and 2 mg/l Kin. Regeneration occurred in some treatments without Kinetin, showing that Kinetin is not essential for Bunium persicum regeneration. The treatment containing 0.1 mg/l NAA and 4 mg/l Kin was the best one for regeneration. The best treatment for somatic embryogenesis was 2 mg/l 2,4-D.
M Mehrabi Koshki, D Zafari, B Sharif Nabi,
Volume 13, Issue 47 (4-2009)
Abstract
Recent studies have shown the controlling effects of Trichoderma species and extract of a few plant species of the Brassicaceae family on some phytopathogenic fungi. In this study, using a completely randomized blocks design in the field, controlling effect of biological agents comprising mustard flour, Trichoderma koningii T18, T. virens T59, T. brevicompactum T30, T. harzianum T56, mixture of four Trichoderma isolates, mustard flour + mixture of four Trichoderma isolates and two commercial biological product of Trichodermin B and Subtilin were evaluated against wheat common bunt caused by Tilletia laevis. According to the infection index, all treatments were able to reduce infection percentage and showed significant differences (P<0.01) compared to control (infected with T. laevis without any biological agent) in which heads infection was 43.5%. Treatments of Mustard flour and mustard flour + mixture of four isolates, reduced the disease by 89.9% and 87.4% respectively. Consequently, it seems that wheat common bunt could be controlled by application of non-infected seeds and by treating seeds using mustard flour without using chemical fungicides.
A Khodashenas, A Koocheki, P Rezvani Moghadam, A Lakzian , M Nassiri Mahallati,
Volume 14, Issue 52 (7-2010)
Abstract
Among the biodiversity of soil microorganisms, bacteria have the basic role in soil functions. In order to determine the diversity and abundance of soil bacteria in arid regions, and also to study the effect of agricultural practices on them, a study was conducted in winter wheat fields on Shirvan, Mashhad and Gonabad. In each region, high and low input fields of winter wheat and a natural system for comparison were selected. Use of agricultural inputs was criteria for selection of low and high input fields in each region. Soil sampling was done on fields and natural systems and organic matter content, abundance and diversity of soil bacteria were measured in soil samples. Species richness and abundance of soil bacteria was affected by region and so that natural system of Gonabad has the minimum of species richness among the studied systems and the differences of other systems was not significant. Abundance of soil bacteria in 1 g dry soil was maximum in Gonabad and minimum in Shirvan. Abundance of soil bacteria was affected by organic matter and in low and high input systems of Gonabad and low input system of Mashhad was greater than other systems. Overall, 19 species of bacteria that belonged to 4 genus were detected. Results showed that species richness and abundance of soil bacteria in studied systems were relatively low and agricultural practices have not significant impact on these organisms, so that species richness and abundance of soil bacteria were improved in agricultural systems of Mashhad and Gonabad. Pattern of bacterial diversity showed that regional and agricultural stresses were affected on bacterial function so that in high level of stresses, species richness decreased and function of soil bacteria was restricted to decomposition of organic matter. Therefore, organic matter of soil must be increased and agricultural stresses must be decreased to improve of soil bacterial functions.
