Showing 6 results for Bacterium
M.r. Lak, M. Shamsbakhsh, M. Bahar,
Volume 6, Issue 1 (4-2002)
Abstract
During the summer of 1998, leaf and pod blight symptoms of bean were observed in Arak, The survey in the following year showed that the disease had an increasing trend in bean-growing regions of Markazi Province. It appeared that more severe losses occurred in fields equipped with sprinkler irrigation system. Symptoms of diseased plants consisted of formation of irregular necrotic lesions on leaves surrounded by yellow halo. Under favourable conditions, these lesions gradually expanded and heavily infected leaves became blighted. The primary symptoms on infected pods were the development of water soaked spots which later turned to dark or reddish blotches. In attempts to investigate the causal agent, two types of a Gram negative bacteria with raised, yellow and translucent colonies were isolated from leaves and pods of blighted beans. All strains produced Xanthomonadin pigment and their inoculation on young bean plants induced disease symptoms similar to those observed under field conditions. Based on morphological, physiological and biochemical properties, the predominant pathogenic type was identified as Xanthomonas axonopodis pv. phaseoli. However, the other strain with the same properties, but capable of producing melanin pigment in nutrient media, was assigned to X. axonopodis pv. phaseoli var. fuscans.
P. Norouzi, D. Cai, M. A. Malboobi, B. Yazdi Samadi,
Volume 7, Issue 3 (10-2003)
Abstract
OF2 and VAP genes, probably involved in signal transduction of sugarbeet nematode resistance, have already been cloned in bacterial vector by AFLP molecular marker and a two-hybrid system, respectively. To examine their capability to introduce resistance in sugarbeet, the genes were transferred to plant expression vectors. For this reason, OF2 gene after isolation was inserted within T-DNA of pAM194 binary vector, downstream of CaMV35S constitutive promoter and also inserted within T-DNA of modified pBin121 binary vector, downstream of HS1pro-1 gene inducing promoter (responsible for nematode resistance). VAP gene after isolation was inserted within T-DNA of pAM194 plasmid, downstream of CaMV35S constitutive promoter. Thus, three new constructs were made in which genes of signal transduction pathway were expressed to give beet cyst nematode resistance. These plasmids were separately transferred to Agrobacterium rhizogenes, strain AR15834. In the next step, petiole explants of sugarbeet were inoculated with the bacterial cells. Transformation-derived hairy roots were analyzed by GUS staining and/or PCR and were then inoculated with nematode larvae. Primary results showed partial resistance against nematode larvae in some hairy roots. As a result, this resistance can be related to OF2 and VAP genes effect.
A. Yamchi, F. Rastgar Jazii, C. Ghobadi, A. Mousavi, A. A. Karkhanehee,
Volume 8, Issue 4 (1-2005)
Abstract
Proline as a key osmoregulating solute in plants plays an overriding role in osmotic pressure adjustment of the cell under water stress conditions. In plant, a bifunctional enzyme delta-1-pyrroline-5-carboxylate synthetase (p5cs) promotes and directs proline synthesis during drought stress conditions. The activity of this enzyme is strongly induced to increase proline concentration within the cell to prevent the impact of water deficit. In this study, the sequence coded for p5cs enzyme under the control of the cauliflower mosaic virus 35S promoter was cloned into a plasmid containing gus and nptII genes. The construct pBI121-p5cs was then transferred into Agrobacterium tumefaciens C58 (pGV3101) and used for producing transgenic tobacco(Nicotiana tabacum cv. Xanthi) plants. The amplification of a 765 bp band within p5cs gene from transferred plants and forming deep blue color in leaf tissues of the explants indicated the successful introduction of p5cs construct into nuclear genome of tobacco plants through Agrobacterium-mediated transformation. The two-month old plants growing under normal condition besides the five-day seedlings under drought stress were subjected to determination of proline concentration. Comparison of P5cs product levels between control and water tolerated plants indicated an increase of proline of about 96.91 to 1330.891 mg/g and of 204.454 to 2039.77 mg/g in plants under normal irrigation and under drought stress, conditions respectively. The significant difference between the levels of proline product in control and transgenic plants under different growing conditions demonstrated the expression of targeted gene (p5cs) in engineered tobacco plants that may pave the way to overcome the water stress problem in agronomically useful crops.
F. Shahryari, G. Khodakaramian, A. Heydari,
Volume 8, Issue 4 (1-2005)
Abstract
Representatives of fluorescent pseudomonad strains were assessed for their antagonistic activity against the blackleg of potato pathogen, Pectobacterium carotovorum subsp. atrosepticum. Tested strains belonged mainly to Pseudomonas fluorescens Biovars III, IV and V and they could produce inhibition zone on PDA medium against P. c. subsp. atrosepticum ranging from 1.5-5.5cm. Six selected strains were used in two concentrations 107-109 and 1010-1012 colony forming units to control potato blackleg disease caused by P. c. subsp. atrosepticum under greenhouse conditions. Results showed that all strains applied could reduce the disease ranging from 46 to 71 percent. There was no significant difference among strains and their two concentrations under greenhouse conditions. Most of the strains applied increased potato fresh weight and all of them produced antibiotic and siderophore compounds in vitro.
A. Esmaili Dastjerdipour, M. H. Farpoor, M. Sarcheshmehpour,
Volume 18, Issue 69 (12-2014)
Abstract
Organic substances produced by cyanobacteria and some polymeric compounds play a role in soil aggregation and increase soil structure stability in sandy soils. Effects of biological soil crusts and some polymeric compounds on some properties of a sandy soil were investigated in this research. Inoculation of three cyanobacterial treatments (Nostoc sp., Phormidium sp. and their combination) and four time intervals on crust formation, organic carbon and calcium carbonate contents, resistance to penetration and MWDD in soils below the crusts were studied in the first experiment. Combination of two cyanobacterial species in 60 days treatment with 2.2 % organic carbon, 0.2 Mpa resistance to penetration, and 96.7 % large aggregates was introduced as the superior treatment. Effect of superior cyanobacteria and time treatments on aggregate size distribution was studied in the second part of this experiment. Sixty-day treatment with 96.7 % of large aggregates was the most effective treatment. Structural stability of a sandy soil influenced by four concentration levels (0, 1, 2 and 4%) of anionic polyelectrolyte polymer at the depths of 1, 2 and 3 cm was investigated in the second experiment. Four percent concentration treatment of polymer with 0.6 Mpa resistance to penetration and 90.9 % large aggregates was the most effective one. A significant difference in resistance to penetration among three depth intervals was also observed
N. Enayatizamir, M. Noruzi Masir, A. Ghadamkhanii,
Volume 23, Issue 4 (2-2020)
Abstract
The soil organic matter plays an important role in increasing agricultural products and various nutrient cycle in the soil due to its effect on the physical, chemical and biological properties of soil. There is, however, little information regarding the effect of growth promoting bacteria on biological indices and different forms of carbon in agricultural soils of the country. Therefore, this study was aimed to investigate the effect of plant growth promoting bacteria on soil respiration, microbial quotient, organic carbon, microbial carbon biomass, permanganate oxidizable carbon, cold water extractable organic C, and hot water extractable organic C under the cultivation of wheat, Chamran cultivar. The experiment was conducted in greenhouse condition as a randomized complete design with 9 replications. Treatments consisted of bacterium inoculation (without inoculation, Enterobacter cloacae Rhizo_33, Enterobacter cloacae Rhizo_R1
and mixof both bacteria). During the experiment, some characteristics such as plant height and chlorophyll index were measured. At the end of the cultivation period, root and aerial part dry weight and grain yield were determined. Biological properties and different forms of carbon in the soil were measured after cultivation. The results indicated the applied plant growth promoting bacteria increased chlorophyll index, height, root and shoot dry weight and grain yield, as compared to the control. The minimum value of pH and the highest amount of each carbon forms were obtained by soil inoculation with different strains of bacteria, as compared to the control. The highest value of organic carbon was observed in the presence of the consortium of both bacteria with 22.7% increase, as compared to the control. The highest amount of microbial carbon biomass was, respectively, measured in the treatments containing consortium of bacteria, Enterobacter cloacae Rhizo_R1, Enterobacter cloacae Rhizo_33 with 87.67, 42 and 26.5% increment, as compared to the control. A positive and significant correlation was observed between cold and hot water extractable organic carbon, dissolved organic carbon and permanganate oxidizable carbon with soil respiration and there was a negative correlation between mentioned properties and the soil pH. The use of microbial inoculants increased the carbon content of the soil, which can play a positive role for improvement of physical and chemical properties of the soil and plant yield.
