Showing 10 results for Biological Control
G.pezeshki-Raad, M. Masaeli,
Volume 6, Issue 4 (1-2003)
Abstract
This study utilized a descriptive-correlational surveying approach to examine some economic factors involved in the adoption of an integrated campaign to control rice stem borer (Chilo suppressalis) in Isfahan Province. The population consisted of farmers living in the vilages where Biological Control Plan was conducted (N=6000). The population frame was obtained from Isfahan Agricultural Organization. The sample was obtained using the cluster random sampling method (n=361). Questionnaire and interview were used for data collection in this study. Face and content validity of the instrument was established using a panel of experts consisting of senior faculty members in agricultural extension and education at Tarbiat Modaress University. Further, the agricultural officer of Isfahan Province validated the questionnaire. A reliability analysis was conducted and Cronbach alpha value was reported to be 83 percent. The results showed that the rate of adoption of Integrated Campaign to Control Rice Stem Borer among the farmers was moderate. Among the economic characteristics, there was a significant relationship between adoption of integrated campaign and area of land, amount of area under cultivation, degree of family cooperation in the agricultural activities, access to agricultural inputs, access to financial resources, and yield of rice per hectare.
S. Ghadiri Rad, B. Hatami, G. Asadi,
Volume 6, Issue 4 (1-2003)
Abstract
The biology of predatory fly, Leucopis glyphinivora Tanas. was studied under greenhouse conditions. Adult longevity was 8 days and the average fecundity was 35.7 eggs. Incubation period, the 1st, 2nd, 3rd larval instars and puparium duration averaged 2.7, 2.16, 3.48, 5.7 and 8.45 days, respectively. Each generation lasted about one month. The predator efficiency was studied by applying the egg together with the adults of A. fabae at ratios of 1:1, 1:2, 1:4, 1:8 and 1:16 on broad bean. First larval instar was exclusively feeding on small nymphs (1st and 2nd nymphal instars) of aphid. Predator:prey ratios of 1:4, 1:8 and 1:16 decreased the number of prey to 97.9%, 98.8% and 61%, respectively, but ratios of 1:1 and 1:2 were unsuccessful in decreasing the number of preys due to predatory larvae leaving the host plant for searching small nymphs of aphid. As a result, the predatory larvae were efficient merely in a particular range of aphid density and age structure.
M. Niknejad Kazempour, H. Pedramfar, S. A. Elahinia,
Volume 6, Issue 4 (1-2003)
Abstract
Antagonistic fungi Trichoderma harzianum (T1, from bean fields in Ahwaz, T2, from rice fields in Rasht), T. viride (T3 from bean fields in Shahriar, T4 from the collection in Plant Pest and Disease Institute, Tehran), Gliocladium virens (from bean fields in Kamal Abad, Karaj) and some fungicides (Benomyl, Carbendazim, Carboxin-Thiram, edifenphos and Zineb) were used to control sheath blight of rice incited by Rhizoctonia solani in vitro and under greenhouse conditions. The experiment was carried out in a completely randomized design with 12 treatments and four replications on Khazar rice cultivar in a soil infected by R. solani under greenhouse conditions. The results showed that the antagonistic fungi reduce sheath blight by 19.8 (T1), 21.5 (T2), 27.5 (T3), 19.6 (T4) and 18.5 (G1) percent. The antagonistic fungi in order of efficacy were T2>T3>T1>T4>G1. Benomyl, Carbendazim, Carboxin-thiram, Edifenphos and Zineb reduced disease by 32.5, 21.5, 12.8, 9.5 and 0 percent, respectively. Statistical analysis of data indicated that there existed no significant differences between T1, T3, T4, and G1 and Carbendazim fungicide to control disease however, the isolate T2 was as effective as Benomyl but Zineb had no effect on sheath blight.
Z. Rafiei Karahroudi, B. Hatami,
Volume 7, Issue 2 (7-2003)
Abstract
Green lacewing, Chrysoperla carnea (Steph.), is one of the important natural enemies of cotton aphid, Aphis gossypii Glover. In this study, two experiments were conducted on cucumber plant in netted cages (70*70*40 cm) in a greenhouse. In the first experiment, Chryso-bags (special net bags with 360 holes per 2.5 cm2 and 8 cm in width and 12 cm in length) were used. Seven treatments of release ratios included 1:1, 1:5, 1:10, 1:15 and 1:20 (predator: prey) and two control treatments with and without empty bags were compared. The experiment was conducted once in spring and once in autumn. In the second experiment, better release ratios were compared with two methods, Chryso-bag and sawdust as a carrier of eggs. Release ratios were 1:1 and 1:5 (predator: prey). Control treatments included only empty bag and sawdust, respectively. Results of the first experiment showed that the ratios of 1:1 and 1:5 were significantly different in spring, whereas they were not in autumn. The difference was probably due to the difference in temperature. Interaction between treatments and seasons showed that the ratios of 1:1 and 1:5 were not significantly different. In the second experiment, the release ratios of 1:1 and 1:5 in sawdust with each other and with 1:1 in Chryso-bags were not significantly different, while the ratio of 1:5 in Chryso-bag compared with 1:1 in both methods were significantly different. However, leaf necrosis was observed in the sawdust treatment.
F. Padasht Dehkaei, Sh. Mansouri Jajaei H. Rouhani,
Volume 8, Issue 1 (4-2004)
Abstract
One hundred and two microorganisms were isolated from 85 paddy soil samples of different locations in Guilan Province. Antagonistic effects of the microorganisms were tested against Gibberella fujikuroi on PDA medium. Twenty one microorganism isolates were selected for seed treatment in blotter test. Seven isolates that were satisfactorily effective in reducing pathogen colonies formed on seeds and seedlings were tested in controlling disease in a greenhouse experiment with split plot design. The antagonists were Bacillus subtilis, B. circulans, Bacillus sp., Trichoderma harzianum, T. virens (2 isolates), and F.23 (unidentified). Results showed that all antagonists effectively reduced the disease in sterilized soil compared with control but overall effectiveness of B. subtilis, T. harzianum, and T. virens was higher than others and lower than the fungicide treatment (thiophant methyl thiram W.P. 80% , 2g/L). It was found that when seeds were treated with antagonists prior to inoculation with the pathogen, the effectiveness of antagonists was significantly better than when they were applied after seed infection with the pathogen.
A. Nasr Esfahani, A. Mousavi Bak,
Volume 8, Issue 3 (10-2004)
Abstract
The black dot disease of potato is a fungal disease caused by Colletotrichum coccodes (syn. C. atramentarium) that produces accervuli on infected underground plant parts which appear as black dots. The disease appears at the flowering stage and develops up to the season end. The survey on black dot disease of potato in two successive years indicated that the infection percentage is 39.86 in Feraydan (Isfahan), one of the most important potato production regions in Iran. Studies on usual cropping patterns with, potato including, wheat alfa-alfa, barley and fallow showed that in terms of disease infection, one year rotation with fallow reduced the disease severity almost to half compared with control and other cropping patterns. Biological seed and soil treatment, with Trichoderma harzianum in various spores density reduced the disease in various degrees significantly and increased the potato plant growth factors including the number of stems, foliage fresh and dry weights, and also the yields in cases of spore applications, depending upon the type and application dosage. Comparative susceptibility assessments of 24 commercial potato varieties to the black dot disease indicated that the variety Desiree was the least concerned in terms of infection, followed by Scort ,Casser, Casmos, Carlita and Moren , where as Marijke, Cosima and Monalisa were highly infected in comparison to other tested verities and the rests of varieties were scored within these two ranges.
F. Shahryari, G. Khodakaramian, A. Heydari,
Volume 8, Issue 4 (1-2005)
Abstract
Representatives of fluorescent pseudomonad strains were assessed for their antagonistic activity against the blackleg of potato pathogen, Pectobacterium carotovorum subsp. atrosepticum. Tested strains belonged mainly to Pseudomonas fluorescens Biovars III, IV and V and they could produce inhibition zone on PDA medium against P. c. subsp. atrosepticum ranging from 1.5-5.5cm. Six selected strains were used in two concentrations 107-109 and 1010-1012 colony forming units to control potato blackleg disease caused by P. c. subsp. atrosepticum under greenhouse conditions. Results showed that all strains applied could reduce the disease ranging from 46 to 71 percent. There was no significant difference among strains and their two concentrations under greenhouse conditions. Most of the strains applied increased potato fresh weight and all of them produced antibiotic and siderophore compounds in vitro.
E. Karimi, H. Rouhani, D. Zafari1, Gh. Khodakaramian, M. Taghinasab,
Volume 11, Issue 41 (10-2007)
Abstract
In order to study the biological control of carnation vascular wilt disease caused by Fusarium oxysporum f.sp. dianthi, 141 bacterial strains were isolated from carnation rhizosphere, and their antagonistic activity was evaluated against fungal pathogen in dual culture method. Among the tested strains, 16 strains showed antagonistic activity seven of them with more activity were selected for further investigation. Based on phenotypic features, strains E31 and E57 were identified as Bacillus cereus E76, E93, E102 and E121 as Bacillus subtilis and E130 as Pseudomonas fluorescens bv. III. All bacterial strains inhibited mycelial growth of F. o. f. sp. dianthi by production of non-volatile and volatile metabolites under laboratory condition. Microscopical analysis showed that all strains caused deformation of pathogen mycelium, and metabolites of these strains reduced conidia production rate and as well as the ability of conidia germination. In the in vivo tests, in sterilized and nonsterilized soils, the effect of bacterial strains was studied on disease severity, percentage of healthy plants and the growth rate of plants using soil inoculating and root-dipping into bacteria-methyl cellulose mixture methods. The E57 and E121 strains, in both methods, and E130 in root-dipping method showed highest effect on decreasing of disease severity and increasing of healthy plants percentage. Strains E57, E121 and E130 significantly increased total dry weight of carnation. Maximum dry weight was obtained by E57 and E130 in soil inoculating and root –dipping methods respectively.
Sh.a. Sarani, A. Sharifi Tehrani, M. Ahmadzadeh, M. Javan Nikkhah,
Volume 11, Issue 42 (1-2008)
Abstract
Two hundred fifty seven bacterial isolates were collected from colza root and rhizosphere in Golestan, Mazandaran, Guilan and Tehran provinces. Antagonistic effect of bacterial isolates on Rhizoctonia solani, the causal agent of colza damping off, was studied using dual culture method. The results showed that, 60 isolates had the ability to inhibit the growth of R. solani on PDA medium. On the basis of the biochemical, physiological and morphological tests, isolates P1, P2 and P3 were identified as Pseudomonas fluorescens. Biocontrol mechanism studies showed that isolates produced antibiotics and volatile metabolites prevented the mycelial growth of the fungus. The isolates produced some of antimicrobial metabolites including hydrogen cyanide, protease and siderophore. Isolate P3 was more effective to inhibit the growth of the fungus in-vitro. The effect of isolates on disease reduction in comparison with control was significantly different. None of the isolates were able to prevent disease occurrence completely. Isolates applied as soil treatment had a significantly higher disease control as compared to seed treatment method. Isolate P3 had considerable effect on reduction disease in the greenhouse conditions. All isolates were capable of colonizing canola roots and so increased canola growth in free-causal agent conditions.
E Mahdikhani Moghadam, H Rouhani, M Flahi Rastegar,
Volume 13, Issue 48 (7-2009)
Abstract
Sugar beet cyst forming nematode (Heterodera schachtii) is one of the most important pathogens of the sugar beet in Iran. For biological control of Heterodera schachtii, 10 isolates of Trichoderma related to two species T. harzianum and T. virens were examined in laboratory and green house on eggs and cysts for two years. Results obtained from the laboratory assay showed that isolates of Trichoderma parasitized 60% eggs on average. Among them two isolates T. harzianum Bi and T. virens VM1with % 76.18 and %72.55 parasitism, respectively, showed more efficiency compared with the control. In green house, experiments were carried out in autoclaved and non autoclaved soils (field soils) separately with 12 treatments and 3 replications including non infested control (using Ragbi nematicide in field soils experiment), and infested control treated with isolates of Trichoderma using Randomized Complete Design. Then analysis of variance for the bio-control potential of isolates, final population of nematode, fresh and dry root weight, fresh and dry leaves weight inoculated with isolates of Trichoderma was carried out. The results revealed a significant difference (P<0.05) between treatments and control according to Duncan,s Multiple Range Test. T. harzianum Bi and T. virens VM1 decreased population of nematodes, and increased yield in autoclaved and field soils. In autoclaved soils, the two isolates (T. harzianum Bi and T. virens VM1) decreased population of nematodes by %76.68 and %72.65, respectively compared with the control. The Ragbi nematicide, T. harzianum Bi and T. virens VM1 decreased population of nematodes by %81.65, %75.15 and %72.85, respectively compared with the control in field soils experiments.
