JWSS - Journal of Water and Soil Science

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Two experiments were conducted to evaluate the nutritional value of millet and tallow for broiler chickens. In the first experiment, the nitrogen corrected apparent metabolizable energy (AMEn) of millet and tallow were determined by the method of Sibbald. In the second experiment, a 3×5 factorial experiment was designed to evaluate the effects of 3 levels of tallow and 5 levels of substitution of corn with millet in broiler diets. Animal fat was included in the diets at levels of 0%, 3% and 6% and millet was replaced for corn at levels of 0%, 25%, 50%, 75% and 100%. Each diet was tested in 4 replicates including 12 chicks per replicate. Live body weights, feed consumptions and feed conversion ratios were determined weekly for all replicates. The AMEn of unground, ground, screened and ground and tallow were 3260, 3204, 3237 and 8166 Kcall/kg, respectively. Replacement of corn by millet at all levels did not have any adverse effect on body weight, feed consumption and feed conversion ratio at 56th day of age. Body weights, feed consumptions and feed conversion ratios were significantly increased (p<0.05) with increasing animal fat. The results indicated that millet has high energy value and appears to be at least equal to corn in promoting weight gains in broiler chickens.

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In a completely randomized design, 600 commercial day old broiler chickens were used to study the possibility of replacing corn with barley. Each of 10 experimental diets in which corn was replaced with barley at levels of 0, 5, 10, 20 and 40 percent, with and without tallow, were given to 4 replicates of 15 chickens each. At 42, 49 and 56th days of age, one male and one female from each pen were killed. Body weight gain, feed intake and feed conversion ratio were not affected by different levels of barley at 42th day of age. Increasing the level of barley had not effect on body weight gain at 56th day of age. Feed conversion ratio improved slightly due to addition of 4% tallow to the diets containing different levels of barley. Abdominal fat was increased due to added tallow in males and females. Increasing slaughter age increased abdominal fat in both sexes. Difference in abdominal fat in females was more than males.

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Effect of different sources of fat on performance of broiler chicks was studied. Three hundred and thirty six chicks were divided into 42 groups of 8 chicks per group. Seven isoenergetic and isonitrogenous diets, mainly based on corn and soybean meal were prepared and fed. These treatments were as follows: corn + soybean meal + (5% tallow, 5% cotton seed crude oil, 5% cotton seed soapstock, 2.5% tallow + 2.5% crude oil, 2.5% tallow + 2.5% soapstock, or 2.5% crude oil + 2.5% soapstock). One control diet without any source of fat was also included. Results showed that average feed intake of diets with different sources of fat up to 56 days old, was not differed from control group. Different sources of fat and their combinations increased weight gain and improved feed conversion. But soapstock had no effect on performance. Added fats increased abdominal fat of chickens. Therefore, if is concluded that on the contrary to soapstock, tallow and crude oil enhanced performance of broiler chickens.

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Barley consumption is limited in broiler chicken diet due to their β. glucanas polysaccharide content. The application of synthetic enzymes in poultry diet can increase the barley content. The purpose of this experiment is to study the effect of enzyme supplement-β-glucanas on the performance of broiler chickens, percentage of abdominal fat, intestine weight and economic evaluation of production of one kilogram of meat in diets including different levels of barley and corn. Five hundred forty-one-day-old commercial broiler chickens (Arian) were divided into 45 groups, 12 chickens per group Each one of the 15 experimental diets, in which barley was substituted for corn at levels 0, 25, 50, 75 and 100% with 2 levels of enzymes (0.025 and 0.05%) was fed to 3 groups of chickens for 49 days. All diets were isoenergetic and isonitrogenous. Plant oil was used to make diets isoenergetic. The results showed that the rate of weight increase of broiler chickens decreased as each level of free enzyme barley was replaced at 0.83, 6.08, 12.9, 16.1 %, respectively. The weight gain of the chickens fed with diets including a large amount of barley and 0.05% enzyme didn’t have any significant difference (P < 0.01) with the control group. The chickens fed with diet including barley and 0.025% enzyme didn’t have any significant difference either (except at 75% and 100% barley replacements). Enzyme supplementary at 0.025% and 0.05% levels (especially at 0.05%) caused significant improvement (P < 0.01) in food consumption and feed conversion ratio of chickens fed with diets including barley. Analysing the carcass of these chickens showed that the enzyme used at both levels, especially at 0.05 %, caused a significant decrease (P < 0.01) in abdominal fat (3.62% vs. 4%). It was also seen that the weight of chickens fed with enzyme decreased significantly (P < 0.01) in comparison with chickens fed with free enzyme barley diets (9.57% vs. 14.81 %). The economic calculation results of producing meat in this experiment pointed out that using barley and corn caused reduction in production expenses. The general results of this study showed that barley with enzyme is a suitable replacement for enzyme and further that 0.05% level of enzyme is better than 0.025% level due to its performance and better economic results.

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In order to study the effects of Total Dissolved Solids (TDS) in water on the performance of broiler chickens, preliminary data were obtained on water resources of Isfahan Province. Complementary tests and analyses were also performed including physical, chemical and microbial ones. Six different water qualities based on TDS contents of less than 1000, 1000-2000, 2000-3000, 3000-4000, 4000-5000 and more than 5000 ppm were selected. The main experiment lasted for 7 weeks (7-56 days), using 288 chickens in a completely randomized design, with 3 replications, while, the environmental and genetic factors were controlled in an identical broiler housing.

The results showed that using water with different TDS contents had the most negative effects on mortality rate (%) in the rearing period and up to 56.2% mortality was recorded at the highest TDS content. Water consumption showed high and positive correlation (P < 0.05) with TDS (r = +0.74). Correlation between TDS and bedding moisture was also high (r = +0.65) and significant (P < 0.05). The differences between final body weight of control chickens and highest level of TDS were significant (P < 0.05). Feed intake also was affected by different TDS contents and showed similar results as with body weight. Increasing the TDS level decreased the abdominal fat in the female chickens and ash content of tibia in the male chickens. It is concluded that water TDS should be considered for the development of broiler chicken farms. Therefore, it is recommended that higher quality waters (with a TDS content of 3000 ppm) must be used for this purpose. Otherwise, appropriate systems should be used to improve physical and chemical composition of the water.

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This experiment was conducted to determine the requirements of vitamin supplement and available phosphorus in broiler chickens from 42 to 56 days of age. In a completely randomized design, with a 3×3×3 factorial arrangement, 270 one-day-old commercial broiler chicks were divided into 27 groups (replicates) with 9 treatments, 3 replicates and 10 chicks in each group. Each of the nine diets was given to each treatment from 42 days of age. Levels of vitamin supplement were 0.5, 0.25, 0% and available phosphorus levels were 0.27, 0.22, 0.16%. Diets were isocaloric and isoprotein. Group weight and feed consumption of each replicate were determined after 42 and 56 days of age and daily gain, daily feed consumption and feed conversion were calculated. Percent of carcass components and tibia ash were determined.

Results indicated that removal of vitamin supplement from 42 to 56 days of age did not have any significant effects on daily gain, daily feed consumption, feed conversion, live weight, viscera, abdominal fat, carcass weight, wings, neck and back bone. Also, reduction of available phosphorus did not have any significant effect on daily feed consumption, feed conversion, live weight, viscera, abdominal fat, carcass weight, breast meat, femurs, wings, neck and tibia ash. Interaction between vitamin and phosphorus were significant for daily gain, daily feed consumption, live weight, wings, neck and tibia ash (P<0.005) and were highly significant for breast meat, femurs, and back bone (P<0.00l).

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This experiment was conducted to study the effects of using cotton-seed meal on the performance and carcass yield of broiler chickens at different ages. The experiment was performed in a completely randomized design with four treatments and five replicates for each treatment. The experimental treatments included the levels of 0, 3.5, 7, and 10.5% of cotton-seed meal in diets fed to chickens at 7-21 days of age. Except for the control group, these levels for grower (21-42 d of age) was increased to 7, 14, and 21% and for finisher (42-56 d of age) to 14, 21, and 21%, respectively. For the purposes of this study, 260 day-old chicks were reared in litter until 7 days of age. On day 7, chicks were divided into 20 pens with 13 chicks in each pen. NRC (1994) recommendation was used in balancing experimental diets. The chickens were weighed weekly throughout the experimental period (7-56 d). The main observations were body gain, feed intake and feed conversion ratio. Physical analysis of carcass was done at the end of the experimental period and percentages of carcass, abdominal fat, liver, pancreas and digestive tract to live body weight were calculated. The use of cotton-seed meal and the increase of the levels through different ages had no significant effects on body weight, daily gain, feed intake, feed conversion ratio, and carcass components. Increasing the level of cotton-seed meal in the diet at 7-21 days of age had significant effects on feed intake (P<0.05) and increased it. The results of this experiment indicate that we can use cotton-seed meal at levels of 7,14, and 21% of diets, respectively, in starter, grower and finisher stages without having any adverse effects on the performance and carcass yield of broiler chickens.

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In a completely randomized design, the effects of six different additive compounds including limestone, hydrochloric acid, zeolite, aluminum sulfate, pad ammoniac, and fatty acids on nitrogen, pH, temperature, moisture, water soluble phosphorus, and microbial count of litter of broiler chicken were investigated. Each compound was added at a level of one kg/m² of litter. Six–hundred-and-sixteen (616) day- old broiler chickens (both sexes) were divided into 28 groups, 22 chicks per group. Four replications were allocated to each treatment from 1 to 56 days. All groups were fed with the same diet. Litter moisture was not affected by the treatment. Experimental treatments significantly (p<0.05) influenced the litter pH on day 21. The lowest pH was obtained with aluminum sulfate and the highest pH was obtained with limestone. At the end of the experimental period, all treatments had nearly the same pH levels. Litter temperature was not affected by the treatments . Experimental treatments had a significant (p<0.05) effect on litter nitrogen and bacterial count. Correlation between litter pH and nitrogen was significant and negative (r = -0.95, p<0.05). Effect of treatments on soluble P was significant and the lowest soluble P belonged to aluminum sulfate and pad ammoniac. Correlation between soluble P and pH was positive (r = 0.48, p<0.05). The results showed that some chemical compounds can be used to reduce litter pH, nitrogen, and soluble P and their effectiveness diminishes as chicks grow older.

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In order to study the effect of corn, Ghods wheat, Falaat wheat, and triticale with or without enzyme addition on perormance and digestive tract characteristics, an experiment was carried out by using 288 male broiler chickens in a compeletly randomized design with 4*2 factorial arrangement for 57 days. Enzyme addition significantly decreased both feed conversion ratio of chickens fed Ghods wheat and triticale, and weight gain of chickens fed Ghods wheat. Growth performance of chickens fed Ghods wheat with enzyme was not significantly different compared with corn without enzyme. Enzyme addition decreased ileum relative weight of chickens. Gizzard and proventriculus of chickens fed Falaat wheat and triticale were heavier than those of other treatments. According to the results, enzyme supplementation showed positive effect on triticale and especially Ghods wheat based diets.

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The purpose of this research was to evaluate the effect of early life nutrient restriction and subsequent intermittent lighting schedule on performance in broiler. In a factorial experiment, the effect of three levels of feeding regimes and two levels of lighting schedule were investigated using 480 day-old male Hubbard chicks. The broiler chickens were randomly distributed over two rooms consisting of 4 replicates of 20 birds for each of three treatment groups. From 3 to 12 days of age treatment groups were either allowed ad-libitum access, 90% or 80% to feed. From 12 days of age until 42 days of age all birds were allowed ad-libitum access to feed. The lighting schedule for day 12 onwards in one room was a 23h light (L): 1h dark (D) lighting schedule (CL) and another room with an intermittent lighting schedule (IL, 1L:3D) from 8 AM until 8 PM . The results indicated that feed intake and feed conversion ratio of birds reared in the IL schedule were significantly lower than CL , at growth and total period of experiment (p<0.01). IL schedule did not have any significant effect on mean body weight at the end of experimental period and body weight gain at total period of experiment(p>0.05). Relative growth rate and production (PI( in the IL were higher than CL schedule (p<0.01). Feed intake and feed conversion ratio of birds given 90 and 80% of ad libitum were significantly lower in comparison with control group (p<0.01). Body weight (gain) at the end of the experiment and growth period and body weight gain in birds fed 80% of ad libitum group were significantly lower in comparison with control and 90% of ad libitum groups. At 5,6 weeks and grower period EPI and relative growth rate in birds fed 80 and 90% of ad libitum were significantly higher in comparison with control. These results indicated that feed restriction and lighting program did not have any significant effect on carcass composition (protein, fat, ash and dry matter), carcass fragment percentage (except for drumstick), abdominal fat percentage, lung , heart, right and left ventricle weight and hematocrit percentage (p>0.05), but proportion of heart weight to body weight and drumstick percentage in birds fed 80 % of ad libitum were higher than the other groups (p<0.05). Lighting schedule and feeding regime did not have any interaction effect, except for relative growth rate (p<0.05). Relative growth rate in birds with feed restriction under IL schedule was higher than birds with feed restriction under CL schedule. Intermittent lighting and feed restriction had synergic, activity effect and positive effect on body weight gain and feed convertion ratio.

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This study was conducted to determine chemical composition and evaluated apparent metabolisable energy (AME) and true metabolisable energy (TME) values of five Iranian barley cultivars. Basal diet contained corn soybean and in each of the test diets barley was substituted for 30% of corn and soybean fractions. The study was carried out in 3 experiments. In experiment 1, metabolisable energy (ME) values were measured by both total collection and markers (chromic oxide) in excreta of 35-d-old chicks. In experiment 2, ME values of the barley cultivars were determined by ileal digesta procedure. In experiment 3, 36 48-d-old broiler chicks (used in experiment 1 and 2) were used to determine TME and the nitrogen corrected TME (TME_n) of the barley cultivars. Highly significant differences (P<0.05) between bioassay methods were observed on average barley ME values. The average barley AME_n value measured by ileal digesta method was significantly (P<0.05) higher than excreta (total collection and markers in excreta) (2716 Vs. 2318 and 2124 kcal/kg, respectively). Barley ME values were influenced by cultivar (P<0.05) and the hullless cultivar showed greater AME value than the hulled cultivars (2695 vs. 2407.5 kcal/kg AME 2630 vs. 2324.7 kcal/kg AME_n). Cultivar had no significant effect on barley TME value. It was concluded that there is high variation between energy contents of Iranian barley cultivars. In addition, ileal digesta was found not to be an accurate bioassay for determination of barley AME value in broiler chicks.

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This experiment was carried out to determine the nutritional value and the effect of different levels of tomato seed and pomace on performance of broilers. Corn grains and soyabean meals were replaced by tomato seed at levels of 6, 12 and 18 % and tomato pomace at levels of 3, 9 and 12 %. At first, the metabolizable energy of tomato seed and pomace was determined by the Sibbald method. In this experiment, 504 commercial broiler chicks (Ross 308) were used for 8 weeks (from 7 to 56 days old ) in a completely randomized design in 7 treatments with 4 replications per treatment. After 21 days, protein digestibility of the ration in illeum was determined. Apparent metabolizable energy was 3394 kcal/kg for seed and 2329 kcal/kg for pomace and crude protein was 31, 22.7, respectively . The result showed that body weight, protein digestibility of rations in illeum and carcass characteristics were not significantly different . Best energy and protein conversion efficiencies were obtained at the level of 6 % seed and 9 % pomace, that were not significantly different from control treatment. Cheeks fed with seed and 12% tomato pomace had significantly (p<0.5) higher feed intake than control group.The difference between diets containing 6% seed and 3% pomace and control group was not significant regarding feed conversion ratio.

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This experiment was conducted in a completely randomized design with five treatments and four replications. There were 15 chicks in each replication during the first 30 days and 10 chicks from day 30 to the end of experiment. Diets were control (without probiotic and dry whey) and levels 500 and 750 gm/ton probiotic, and each level contained two diets with and without %2 dry whey. Daily weight gain, feed intake, feed conversion rate at the end of each experiment and carcass characteristics including weights of neck, Liver, wing, heart, and abdominal fat on the 49th day of experiment were measured. Chicks which received diets with 500 and 750 mg/ton probiotic +%2 dry whey were significantly heavier than other chicks (p<0/05) between 0-21 days. Feed intake significantly increased during 3 periods including 0-21, 21-42, and 42-49 days of experiment, when chicks used diets with dry whey, especially diets containing 750 gm/ton probiotic (P<0/05). Blood plasma cholesterol significantly decreased when chicks used diets with dry whey. Also an increase in white cells was observed in days 21 and 35. Treatments did not have any significant effect on feed conversion rate, mortality and carcass characteristics (P>0/05).