JWSS - Journal of Water and Soil Science

---

To evaluate the effect of broiler growth rate and feed intake on development of the inflammatory response‚ commercial broiler diets were fed to 275 day–old chicks of the modern 2000 strain and 1957 random-bred strain. Half of the chicks had free access to feed while others were restricted. Twelve birds from each strain and group pens were removed‚ weighed, and housed in individual cages by their original feeding programs on 0‚ 7‚ 14, and 28 days. Three feed-restricted and three ad libitum birds from each strain were injected with 1-3 ml of 100 μg/ml solution of Salmonella typhimurium lipopolysaccharide on days 6‚ 13‚ 27‚ and 41, and 3 birds of each group were not injected‚ serving as controls. All birds were weighed and the liver‚ breast muscle‚ thymus gastro intestinal tract and bursa were removed on the day after injection. The thymocytes of the control birds were subjected to an interleukin–1 responsiveness assay. The 1957 strain birds were more severely affected by an inflammatory challenge going almost completely off feed‚ but the 2000 strain birds experienced a more drastic decline in growth rate after injection. Thymocytes of ad libitum birds were more sensitive to IL-1 than the restricted birds in week 4 (P=0.056). Interleukin-1 stimulated thymocytes of 2000 birds more effectively than the 1957 strain ones. In conclusion‚ the broiler inflammatory response was affected by growth rate and feed intake.

---

A chick bioassay with chemically defined amino acid (AA) diets was conducted to compare four different AA profiles: the NRC 1994, Feedstuff 2002, Rhone Poulenc Animal Nutrition 1993(RPAN) and Illinois Ideal Chick Protein (IICP) AA profiles. This battery study involved male and female chicks during 7 to 21 days of age. Indispensable AA were rationed to lysine according to requirement ratios presented in the four profiles. Digestible lysine set at 1.07 and 0.98 % of diet for male and female respectively. This experiment was carried out in a completely randomized block design using 2 5 factorial arrangement with for replicate per each diets were kept isonitrogenous (2.6 % N ) by varying levels of L-glutamic acid. All diets were checked to have at least 0.3 % proline and 0.6 % glycine. Diets for all profiles contained 3200 kcal ME/kg and a positive control diet were used according to NRC 1994 recommendations. Chicks fed a common corn-soybean meal diet for 160 h and were raised in the floor pen. Then chicks weighted individually and allocated to battery pens so that most uniformity between pens occurs. Four battery pens of five chicks were fed one of four different profiles or positive control diet in both male & female. Weight gain and feed efficiency measured for each pen at day 21. Results indicate an improved weight gain and feed efficiency in male rather than female (p<0.5). Chicks fed positive control diet weighted more (p<0.5). Between semi purified diets chicks fed diets formulated with NRC AA ideal ratios had significantly (P<0.5) better weight gain and gain: feed in both sexes relative to IICP and RPAN, but not to Feedstuff. RPAN had worst weight gain and feed: gain in females. Results of this experiment indicated that new ideal ratio of theronine (relative to lysine) in IICP for starter period may be under-estimated.

---

This experiment was carried out to determine the effects of different levels of (Kabuli) chickpea grains with different processing methods on the performance of broilers. In a factorial experiment with completely randomized design, 576 Rose broiler chicks were divided into 16 treatments with three replications of 12 chicks each. The first factor comprised three levels of chickpea (10, 20 and 30 percent) in ration, and the second factor five processing methods of chickpea (raw, cooked for 10 and 20 mins, and soaked for 24 and 48 hrs). Treatments included one control ration (without chickpea) and 15 experimental rations, all of which were isoenergetic and isonitrogenous. The experimental period lasted 8 weeks for the 1 to 56 day old chicks. Body weight gain, feed intake, feed conversion ratio were determined at three age periods of 7 to 21, 7 to 42 and 7 to 56 days. Carcass, abdominal fat, intestine and pancreas weight percentages to live weight were measured. Maximum body weight gain belonged to chickens fed on %30 chickpea grains soaked for 48h in age periods of 7 to 42 and 7 to 56 days, which was significantly different from control diet (P<0.05). The effect of chickpea grains levels and processing methods on chicken feed intake in age periods of 7 to 56 days was not significant. Interaction effects of chickpea levels with processing methods on feed conversion ratio in three age periods was not significant either. The effect of chickpea grains levels and processing methods on carcass, abdominal fat and intestine weight percentages to live weight were not significant except for the pancreas weight percentage (P<0.05). Therefore, it seems that chickpea grain is a suitable source of energy and protein in broiler diet (20% as raw or 30% as soaked for 48% h) when soyabean meals and corn grains are unavailable.

---

A feeding trail was conducted to evaluate the effects of diets contaminated with Aflatoxin B1 (AFB1) on the performance and blood enzymes of broiler chickens. In a randomized complete-block design with 4 blocks and 4 treatments, 112 day-old male broiler chicks were divided into 16 groups, 7 chicks per. Treatments consisted of three levels of AFB1 in starter diet (0.4, 0.8, and 1.2 ppm) along with a control group (no AFB1). Feed intake and body weight gain were recorded on a weekly basis. On days 7, 14, 21, and 28, one bird from each replicate was weighed, and killed to collect the blood samples, and to weigh its proventriculus, gizzard, duodenum and pancreas, heart, liver, spleen, bursa of fabricius, and brain. Feeding AFB1 significantly decreased feed intake and body weight gain and increased relative weight of liver (p<0.05). Relative weight of brain decreased on day 7 and then showed a significant increase by day 28 (p<0.05). AFB1 significantly (P<0.05) increased serum activity of aspartate amino transferase (AST) and alanine amino transferase (ALT) and decreased serum activity of lactate dehydrogenase (LDH) enzymes. Generally, this study demonstrated that, alongside other negative effects, AFB1 could also have adverse effect on the brain of broilers.

---

This experiment was conducted to compare the effect of probiotic thepax^® in diets with different quantities of protein on the performance of Cobb-500 broiler chickens and their serum antibody level against Newcastle disease vaccine (NDV). Two basal diets were formulated based on NRC (1994) recommendations which had NRC (sufficient protein diet) and 90% NRC (low protein diet) for protein. The basal diets were then supplemented with 0 and 0.1% probiotic, resulting in a 2×2 factorial arrangement with 2 levels of probiotic (0 and 0.1%) and 2 levels of protein (NRC and 90% NRC). Each of the 4 dietary treatments was fed to six replicate groups of 20 chicks. The results of experiment showed broilers fed low protein diet without supplementation of probiotic had lower performance than other treatments. Supplementing low protein diet with 0.1% probiotic significantly improved body weight gain and feed conversion ratio, as did those which were fed diets with sufficient quantities of protein. Reducing dietary protein level increased abdominal fat content, but had no significant effect on other carcass composition. Supplementing of probiotic significantly reduced abdominal fat percentage (P<0.05). The utilization of probiotic in low protein diet resulted in increasing serum antibody level against NDV, but sufficient protein diet produced no such effect. Also, the results of experiment indicated that probiotic added to low protein diet improved broilers performance and immune response. However, these effects were not observed in adding probiotic to diet with sufficient quantity of protein.