JWSS - Journal of Water and Soil Science

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Twenty-two surface soil samples (0-20 cm) from Fars province were extracted by five extractants (i.e., DTPA, 1.5 M NH₄H2PO₄, 0.1 NH₃PO₄, 0.05 MNa2EDTA, and 1% Na₂EDTA) and used in a 7-week greenhouse experiment which was a 22 × 3 factorial with 22 soils (clay 16-63% pH 7.8-8.2 electrical conductivity 0.32-1.78 dS m^-1 calcium carbonate equivalent 28-63% organic matter 1.1-4.9% cation exchange capacity 9-27 cmol kg^-1 and DTPA-extractable Mn 4-24 mgkg^-1), 3 levels of applied Mn (0, 10, 20 mg Mn kg^-1 as manganese sulfate), 3 replications, and soybean [Glycine max (L.) Merr., cv. Williams]. Application of Mn significantly increased top dry weight by 4 to 104%, averaging to about 12% over the control. But the increase in Mn concentration was not significant. Total Mn uptake also significantly increased but the apparent recovery of the applied Mn was very low (i.e., 0.05%). Soil pH, calcium carbonate equivalent, and clay content were found to be the properties influencing the extractability of Mn. The DTPA-extractable Mn, alone (with a critical level of 13 mg Mn kg^-1) or along with some soil properties, was the only form of Mn that could reasonably predict the plant responses.

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The presence of large amounts of calcium in the equilibrium system of calcareous soils of arid and semi-arid regions affects the chemical reactions of soluble and exchangeable phases. Exchange phenomenon is one of these chemical reactions in which calcium plays an important role. K-Ca equilibria are important equilibria in calcareous soils and may be an answer to many problems in soil chemistry and fertility, specifically on the availability of potassium. Little work of this nature has been done in Iran. In this work, a variety of calcareous soil samples from northern Khorasan were examined. First, regression equation between potassium adsorption ratio (KAR) and exchangeable potassium ratio (EKR) in 26 soil samples was found to be EKR=0.02+2.48KAR, R²=0.77. Gapon exchange selectivity coefficient (K_G) was estimated as 2.48 for the soils. Furthermore, in 14 calcium saturated soil samples, different concentrations of K were added until equilibrium was reached and EKR and KAR relations were determined for each soil with a regression coefficient higher than 0.91. K_G ranged between 1.21 to 3.34. For the potassium range used in the soils studied, K_G was constant and almost matched the KG obtained from EKR and KAR relation of the first step. Based on this equation, the effect of soluble K on exchange K in the presence of Ca and Mg as dominant cations in calcareous soils may be evaluated.

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Excessive application of sewage sludge leads to the accumulation of potentially toxic elements in soils. The objective of this greenhouse study was to examine the DTPA–extractability of Fe, Zn, Cu, Mn, Pb, Cd, Ni, and Co in relation to soil pH and to investigate the concentrations of these metals in corn (Zea mayz). The study was conducted using a factorial experiment in a completely randomized design with three replications. Sewage sludge was applied at 0, 50, 100, and 200 t/ha on four soils [Langaroud (pH= 4.8), Lahijan (pH= 5.7), Rasht (pH= 6.8), and Isfahan (pH= 7.9)]. Application of sewage sludge significantly increased DTPA-extractable Fe, Zn, Cu, Pb, Cd, and Ni in all soils. The increasing effect was in accordance with sludge level. Langaroud soil had the highest and Isfahan soil the lowest levels of DTPA-extractable metals. Application of sewage sludge increased plant growth and metals in the foliage of corn plants. The metal concentration in corn tissues also increased with decreasing soil pH. Overall, the application of sewage sludge as an organic material, particularly in acid soils, may increase availability of heavy metals, which may, in turn, result in soil pollution. Therefore, addition of sewage sludge to soils should be managed on the basis of the changes in soil heavy metal concentration.

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Knowledge of forms of phosphorus (P) is important in the evaluation of soil P status and also in understanding soil genesis and fertility. Amounts and distribution of P in different organic and inorganic fractions were examined in 39 soil samples of Fars province. Soils were sequentially extracted to determine organic P and inorganic P fractions. Total P with an average of 597 mg/kg soil was lower in the light soils (482 mg/kg) as compared with the heavy soils (690 mg/kg). Organic P constituted considerable portions of TP (27%). Calcium phosphates with an average of 424 mg/kg soil and being comprised of 71% inorganic P was the dominant form of soil phosphorus. Simple regression analysis indicated that there were significant positive relationships between organic P, Ca phosphates and Al-Fe phosphate values with CEC and clay content. Stepwise multiple regression analysis showed that a combination of soil properties such as clay content and equivalent calcium carbonate (CCE) increased correlation coefficients (R). Alghough multiple regression model was significant (at P<0.05), the relative contribution of each soil property in Al-Fe-P fraction was not considerable.

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Information about forms of zinc (Zn) is important for the evaluation of its status in soil and also understanding of the soil fertility and chemistry. To obtain such information, amounts and distribution of Zn in different fractions of 20 soil samples of Tehran province, Iran were determined by sequential extraction method and their relationships with each other and with soil characteristics were investigated. Total Zn of soils ranged from 70 to 169.9 mg/kg^-1. The amount of different Zn forms relative to the sum of forms was determined to be as follows: organic less than 0.1, soluble+exchangeable 0.1, manganese-oxide-associated 0.9, carbonate 1.6, crystalline iron-oxide-associated 3.8, amorphous iron-oxide-associated 4.2, and residual form 89.3%. Simple correlation coefficients showed that pH had a negative significant correlation with all forms of Zn except organic. Silt and clay contents had significant positive correlations with total, residual, and crystalline iron-oxide-asociated Zn but CEC had significant correlation only with total Zn. Calcium carbonate equivalent showed a significant correlation with soluble+exchangeable, manganese-oxide-associated and amorphous iron-oxide-associated Zn. DTPA-extractable Zn was significantly correlated with soluble+exchangeable, carbonate, and amorphous iron-oxide-associated Zn. A significant correlation was also observed between the Zn forms themselves, which is presumably a reflection of the existence of a dynamic relation between the chemical forms of an element in soil.

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Study of phosphorus reactions over time and the role of organic matter in the calcareous soils are important for the development of P fertilizer and manure management practices. The objective of this study was to determine the effect of applied manure on P availability and its chemical forms in the soil, over time. Eight samples were collected from semi-arid calcareous soils of Mashhad plain. The samples were treated with two levels of inorganic P (0 and 300 mg P kg^-1 soil as KH₂PO₄) and two levels of organic matter (0 and 1% cattle manure). The experiment was conducted in a completely randomized design with factorial arrangement. The treated soil samples were incubated for 2, 5, 10, 30, 60, 90 and 150 days, then analyzed for available P (Olsen-P). The result showed that only 17% of added phosphorus was available in P treatment at the end of experiment. In manure treated soils, this figure reached 34% for the same period of time. Application of manure along with P increased the recovery of applied P and CBD-P (Citrate-Bicarbonate-Dithionite). This may be due to the formation of P-organic complexes with Fe oxides. Application of manure in soil increased NaCl-NaOH-P considerably compared with P and P+OM treatments. It can be concluded that P originating from manure compared with inorganic-P may be more available for plants over the time.

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In order to investigate mineral manganese forms and their relations with plant responses, 22 soil samples from Fars province (0-20 cm) were in a greenhouse experiment filled in 3 liter pots, and soybean (Glycine max (L.) merr.,CV.Williams) was grown for 7 weeks. Chemical forms of manganese were determined in soils by warden and Reisenauer sequential extraction method (readily soluble, weakly adsorbed, carbonate bound and oxide bound extracted with Ca(No₃)₂, Ca DTPA +Na₂B₄O₇, HNO₃ and NH₂OH. HCl, respectively) after harvesting the plants. Results showed that the amount of Mn in different forms was in the following order: Carbonatic Mn >Mn Oxides >Weakly adsorbed Mn > Soluble Mn. Regression equations between soil properties and Mn forms showed a significant correlation between calcium carbonate equivalent and carbonate bound Mn. Significant correlation between Carbonate bound Mn and concentration of Mn in the aerial part of soybean showed that this fraction plays an important role in plant nutrition.

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  Studying soil phosphorus fractions is useful in understanding soil pedogenesis as well as soil fertility. In this reseach, 20 soil samples were taken from different depths of 0-30 and 30-60 cm of upper-slope, mid-slope and lower-slope positions of four toposequences in arid (Jei and Ziar in Isfahan) and semiarid (Farokhshahr and Shahrekord) regions. In each toposequence, the soil depth was relatively low at the upper-slope position and increased toward the lower-slope. The soils in upper-slope and mid-slope were under scarce native vegetation where the soil in lower-slope was under farming activities. Soil phosphorus (P) fractionation was carried out using sequential extraction. The total soil phosphorus was in the range of 302-1135 with an average of 715 mg/kg. About 65-89% of total phosphorus were inorganic, and 11-35% organic. The amount of inorganic and organic P in the soil samples was in the range of 204-897 with an average of 571, and 70-238 with an average of 114 mg/kg, respectively. The amount of total, inorganic and organic P increased from upper-slope toward the arable lands and decreased from topsoil to subsoil in all toposequences. In the studied soils, apatite ( Ca₁₀-P ), aluminum phosphates(Al-P), octacalcium phosphates( Ca₈-P ), iron phosphates (Fe-P), iron oxides occluded phosphates( OC-P ) and dicalcium phosphates( Ca₂-P ) were the major constituents of the soil inorganic phosphates, respectively.

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Potassium (K) fixation by phyllosilicates clay minerals is a very important process influencing the availability of K for plants. Information about K fixation is limited in Hamadan soils. The objectives of this research were to determine potassium fixation capacity (PFC) and potassium fixation index (PFI) of 10 surface soils of Hamadan province and the correlation of these parameters with soil charge characteristics. Potassium fixation capacity was obtained using six different levels of added K as KCl and three wetting and drying cycles. Also charge characteristics of soils were determined. Tetrahedral CEC of soils was determined after saturation of samples with LiCl and heating at 300 oC to reduce octahedral charge near zero. The results showed that tetrahedral and octahedral CEC ranged from 4.6 to 16.0 and 0.8 to 4.9 cmolckg-1 respectively. Mineral and organic portion CEC ranged from 6.1 to 20.9 and 0.97 to 9.7 cmolckg-1 respectively. The amount of PFC increased with increasing added K. Mean amount of PFC ranged from 58.2 to 175.03 mgkg-1. Potassium fixation index ranged from 0.23 to 0.67. The results of correlation studies indicated that PFI were significantly correlated with octahedral CEC, mineral and organic portion CEC, total CEC, exchangeable and nonexchangeable K. The results of this research showed that PFI very different in soils. So that this parameter should be consider in K soil testing.

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Although the crucial function of earthworms in improvement of soil physical properties is well -know, but very little is known of the interactive influence of earthworms and organic materials on soil properties such as soil aggregate stability, particularly in arid and semi-arid soils. The low organic matter content and the significant role of earthworms in improving physical properties of arid and semi-arid soils necessitate studying the interactive effects of organic materials and earthworms. Thus, the main objective of this study was to identify the interactive effects of anecic earthworm (Lumbricus terrestris L.) and various organic residues (including alfalfa, compost, mixture of alfalfa and compost and cow dung) on soil aggregate stability expressed as the Mean Weight Diameter (MWD), Geometric Mean Diameter (GMD) and Aggregation Ratio (AR), and furthermore soil Ca and Mg contents. The experiment consisted of a 2×5 factorial treatment organized in a completely randomized design with four replications under controlled greenhouse conditions, lasted for 150 days. Results showed that earthworm inoculation and organic materials addition alone increased significantly all the indices of soil aggregation and aggregate stability, and Ca and Mg contents. However, the combined use of earthworms and organic residues resulted in more stable aggregates. Results indicated that earthworm inoculation in the presence of organic materials resulted in 39, 58, 2, 67, 43 and 74% increases, respectively in MWD, AR, GMD, Ca, Mg and macroaggregates whereas microaggregates were reduced by 13.5% in earthworm-worked soils. We observed a significant relationship (R2=0.945) between soil Ca content and MWD, demonstrating that earthworms apparently excrete calcite that helps bonding clay particles and soil organic matter via cationic (Ca+2) bridging. In summary, results of this study show that the simultaneous applications of anecic earthworms and organic materials may considerably help in improving the structure of arid and semi-arid soils with low carbon level.

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The positive and beneficial effects of mycorrhizal symbiosis on the growth of various plants have already been documented. Most arable crops can mutually establish symbiosis with endo-mycorrhizal fungi, however, mycorrhizal associations with almond (prunus amygdalus), particularly in calcareous soils of arid and semi-arid regions, have not been studied. Thus, to realize the symbiotic association between mycorrhiza fungi and economically important native almond genotypes in Chaharmahal va Bakhtiary province, an experiment consisting of a randomized complete factorial design with three factors: almond genotypes (Mamaei, Rabee, Talkh, Sefid), soil phosphorus levels (0 and 150 kg P ha-1) and mycorrhizal treatments (almond inoculated with Glomus intraradices, Glomus mosseae and without inoculation) with three replications was conducted under greenhouse conditions by 4 months. Results showed that physiological traits including chlorophyll concentration, net photosynthetic rate and water use efficiency, were significantly increased for 20%, 300% and 300%, respectively in mycorrhizal almond plants, while transpiration rate was decreased (8-10%). Although the genotype of almond and soil P were dependent on these factors, mycorrhiza species had the same effects. Increased available P in the soil enhanced the growth in all treatments and photosynthesis of nonmycorrhizal almonds.

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The petroleum refinery sludge is an important source of environmental pollution. Burning and burying of the sludge may have adverse effects on environment and human health. Thus, other mechanisms for decreasing the toxic effects of hydrocarbon substances in the sludge must be used. In this study, Isfahan refinery sludge was dewatered, air dried and mixed by 0, 10, 20, 30 and 40% w/w ratio with two calcareous soils, viz., Mahmoud Abad (Typic Haplocalcids with clay texture) and Bagh Parandegan (Anthropic Torrifluvents with silty loam texture). Different mixtures of soil and sludge were farmed for 21 days and irrigated on a daily basis to field capacity. Then, 100 seeds of Tallfescue (Festuca arundinacea) and Agropyron were planted in polluted soils with 3 replicates in 3 kg pots for 5 months. Result showed that Tallfescue and Agropyron yields decreased in sludge contaminated treatments. In the 40% sludge treatment, Tallfescue decreased the total petroleum hydrocarbons content by 65 percent. The highest degradation for agropyron was in the 30% sludge treatment which showed about 55% reduction in total petroleum hydrocarbons. The 40% sludge treatment resulted in the minimum yields of root and shoot plants. The highest degradation of TPHs occurred in the Tallfescue rhizospher of 40% sludge. Maximum degradation of TPHs on the Agropyron rhizospher was in 30% sludge mixed with Bage parandegan soil, but maximum yield of plant was in 20% sludge. Our study shows that Tallfescue rhizospher is most effective for decreasing TPHs, and that the phytoremediation in soils with more clay can adsorb and fix the toxic components and then at higher levels of pollutions can let the plants grow.

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Microbial transport in soil is critical in different ways, especially in groundwater contamination and bioremediation of groundwater or soil. The main objectives of this research were quantitative study of bacterial transport and deposition under saturated conditions in calcareous soils. A series of column leaching experiments were conducted. Breakthrough curves (BTCs) of Pseudomonas fluorescens and Cl- were measured. After leaching experiment the bacteria was measured in difference layers of the soil columns. The HYDRUS-1D one- and two-site kinetic attachment-detachment models were used to fit and predict transport and deposition of bacteria in soil columns experiments. The results indicated that two-site kinetic model leads to better prediction breakthrough curves and bacteria retention in the calcareous soil in comparison with one-site kinetic model. Interaction with kinetic site 1 was characterized by relatively fast attachment and slow detachment, whereas attachment to and detachment from kinetic site 2 was fast. Fast attachment and slow detachment of site 1 was attributed to soil calcium carbonate that has favorable attachment site for bacteria. The detachment rate was less than 0.01 of the attachment rate, indicating irreversible attachment of bacteria. Most of the cells were retained close to the soil column inlet, and the rate of deposition decreased with depth. Microbial reduction rate for the soil was 4.02-4.88 log m-1. High reduction rate of bacteria was also attributed to soil calcium carbonate that has favorable attachment site for bacteria.

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Contamination of agricultural soils by heavy metals is a serious threat from both agricultural and environmental standpoints. Among heavy metals, cadmium (Cd) toxicity for humans and plants is of great concern due to its high mobility and phytoavailability in soil even at low concentrations. Opposite to Cd, Phosphorus (P) is an essential nutrient for plant growth. A greenhouse experiment was carried out in a completely randomized design to examine the influence of Cd, P and their interaction on the growth and chemical composition of spinach grown on a calcareous soil. Treatments consisted of four Cd levels (5, 10, 20 and 40 mg/kg soil as cadmium sulfate) and four P levels (0, 20, 40 and 80 mg/kg soil as mono-calcium phosphate) in three replicates. The results indicated that 40 mg Cd significantly decreased spinach dry weight by 47% but P application decreased detrimental effect of Cd on spinach dry weight. Also, phosphorus application significantly decreased Cd concentration in spinach aerial parts by 78%. Increasing Cd levels increased concentration of this element in spinach shoots. Addition of 40 mg Cd decreased P concentration by 21.5% in aerial parts. Zinc (Zn), manganese (Mn), calcium (Ca), and nitrogen (N) concentration significantly decreased with P application but increased concentration of sodium (Na). Cadmium application decreased Zn concentration but increased Ca, Na and N concentrations in spinach. Our tentative conclusion is that P application in P-deficient soils is probably effective in decreasing Cd concentration and the detrimental effect of Cd on spinach growth, indicating a negative interaction between these elements. Prior to any fertilizer recommendation, the results of this experiment should be verified under field conditions

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Organic acids can affect phosphorus (P) sorption. In order to evaluate the effect of citric acid and oxalic acid on phosphorus sorption characteristics in calcareous soils, five different soil samples (0-30 cm) were collected in West Azerbaijan province. Soil samples (2.5 g) were equilibrated with solutions of KH2PO4 containing 0 to 20 mg P l-1. Five mM organic acids were added to each tube and sorbed P was calculated. The sorption data were fitted to Langmuir, Freundlich and linear equations. The results showed phosphorus sorption data were well described by the Freundlich and linear equations. The average values of Kf (distribution coefficient), n and equilibrium buffering capacity (EBC) of linear equation in citric acid treatment were reduced to 29, 12 and 17 percent, respectively and for oxalic acid reduced to 43, 27 and 14 percent, respectively. Maximum sorption was reduced significantly by oxalic as compared to citric acid. Standard phosphorus requirement (SPR) was decreased by 57 % as a result of organic acids additions compared to control condition. It is concluded that organic acids additions to soils containing more calcium carbonate and high amount of clay can decrease P sorption.

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Transport of fluoride and consumption of groundwater with excess fluoride concentrations poses a health threat to millions of people around the world. The objective of this study was to simulate transport of fluoride (F) using HYDRUS-1D model. The study was conducted in lysimeters at Lavark research station site in Isfahan. The treatments consisted of two concentrations of F (157 and 315 mg kg-1). The duration of the study was 125 days. Some of soil physical and chemical properties, soluble F and total F concentration were determined during the study. The results showed the transport of F in calcareous soil profiles. This may be due to the high pH and desorption of F ion as a result of repulsion by the more negatively charged soil surfaces. The highest concentration of total F and water soluble F were observed in the 10 cm surface soil layer. The concentration of F decreased with increased soil depth. The correlation coefficient was significant between the water soluble fluoride and the total fluoride (1% level). Also, the difference between the observed t- value and a critical value on the t distribution is statistically insignificant. It showed that the model simulated successfully water soluble F concentration in the soil profile.

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This study was conducted to investigate the effects of poultry manure (PM) and its derived biochars on chemical properties of a sample calcareous soil. Poultry manure and its derived biochars at 200(B₂₀₀), 300(B₃₀₀) and 400(_B400)°C were incorporated with 400 g of soil at 2% level (w/w) and incubated for 150 days. Some chemical properties of soil and bio-availability of some nutritional ingredients such as phosphorous, potassium, iron, manganese, zinc and copper were determined at different times of incubation. Soil nutrients availability, organic carbon (OC), electrical conductivity (EC) and cation exchange capacity (CEC) increased by addition of all these organic substances. Biochars prepared at higher temperatures were more effective in increasing soil OC and its durability. Addition of PM and B₂₀₀ decreased soil pH, whereas B₄₀₀ increased it. Although highest soil EC was observed in B₃₀₀ and B₄₀₀ treated samples in the early stages of incubation, the rate of increasing in soil EC was higher at PM and B₂₀₀ treated samples. In general, it was concluded that biochar prepared at 300°C had the highest effect on availability of nutrients and their durability in the soil. 

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The status of soil K forms and its relationship with K content in wheat grain at harvest period may be important for nutrient fertility management. For this purpose, 40 surface (0-20 cm) and subsurface (20-40 cm) soil samples and also grain samples from wheat fields of Darab region were randomly sampled in 2014. Then physicochemical properties of the soils and the contents of soluble, exchangeable and non-exchangeable K and contents of K in wheat grains were determined. Results indicated that content of soluble, exchangeable and non-exchangeable K in the studied soils ranged 15-70, 91-443 and 396-1182 mg kg^-1, respectively. Significant relationships were obtained between soil K forms and clay, calcium carbonate and CEC. Although it is expected that content of easily available K (soluble and exchangeable K) was low at late stage of wheat growth, most soils had sufficient K content. The positive and significant relationship among different K forms was indicative of the K forms equilibration at the time of wheat harvesting. Although there is not a relationship between K content in wheat grain and soil K forms, it was shown that K content of wheat grain was correlated with soil exchangeable K and clay content in fine-textured soils (clay > 30%). It means that ammonium acetate may be a suitable extractant for estimation of soil K status and plant available K of heavy-textured soils in the studied region.

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This study was conducted to investigate the effects of poultry manure (PM) and derived biochars on phosphorous (P) availability and apparent recovery in a calcareous soil. Treatments consisted of four rates of P (0, 30, 60 and 90 µgg-1) and five organic substances (blank, poultry manure and derived biochars at 200, 300 and 400°C). organic substances were incorporated with 400 g of soil at 2% level (w/w). All soil treated samples plus control were incubated for 150 days. Soil P availbility determined at 8 different stage of incubation time period. Phosphorous availability was less in untreated soil samples with organic substances and also decreased with time. Although P recovery from inorganic P fertilizer was high in the early stages of incubation time compared to treated soil samples but decreased with time, if not treated with organic substances. Phosphorus availability and recovery increased with time in PM and biochares treated soil samples. Simultanous application of inorganic P and organic substances decreased apparent P recovery. Negative interaction obsorved between organic substances and high rates of inorganic P fertilizer on P availability. It was concluded that PM biochar prepared at 300°C had the highest effect on adjusting P availability in calcareous soil.

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