JWSS - Journal of Water and Soil Science

---

Short-term changes (5 years) in canopy cover, species composition and forage production were studied under protection from grazing and grazed condition in 19 range sites in Fereydan region, Isfahan province. In this study, density, canopy cover and forage production data were obtained from quadrates inside and outside exclosures in the spring of 1983. These parameters were studied again in 1988 and compared with the previous data. Results indicated that species diversity in these range sites was relatively high up to 212 plant species were identified. Sixteen, 8.5 and 75.5 percent of these species were classified into I, II and III palatability classes respectively. After five years, comparison of inside and outside exclosures indicated an increase in the density of species of I, II and III palatability classes by 138.8, 120.8 and 51.5 percent inside exclosures, respectively. Density of all species had increased by 62 percent as a result of 5-year protection from grazing. Species composition data as canopy cover were used to construct 3 dendrograms to investigate the similarity among range Sites. Assuming 32% similarity as the threshold level, 19 range sites were classified into 10 vegetation types in 1983. After 5 years, these vegetation types decreased to 8 and increased to 11 under protection from grazing and grazed conditions, respectively. The average forage production inside exclosures was almost twice as much as in grazed sites (600 Vs. 315 kg/h). Three groups of range sites were recognized according to the time requirement for rehabilitation by grazing protection. The first group was improved considerably by the end of the 5-year protection Period. The second group improved relatively within five years of grazing exclusion. The third group did not improve by 5-year grazing protection and needed much longer protection period or some other range rehabilitation practices.

---

Astracantha adscendens is an endemic species in Iran growing on alpine and above alpine timberline habitats on the Zagross Mountain Range. These habitats are characterized by steep slopes, heavy snowfalls and long ice formation periods. The present study was carried out in Chelgerd, Bakhtiari, and Fereidan, Isfahan. Slopes, elevation above sea-level, and magnetic north azimuth were measured. The canopy cover was also measured along four radii in upward, downward, left and right directions. Regression analysis was performed for the measured values of plant and environmental factors.

The results revealed that the upward radius had a high negative correlation with slope changes while the downward radius showed no relationship with slope variations. The two left and right radii had a high and positive relationship with each other, both reducing in length as the slope steepness increased. Shrub volume decreases with increasing slope steepness. Plant shape was classified into seven groups using Sorenson similarity index and constructing the dendrogram. Snow pressure bends the stem toward the soil surface. Snow gliding pressure scratches stem and its base buds above the bent stem. Soil and debris move downward the slope as a result of snow gliding and rainfall runoff as well as wildlife and domestic animals. Snow gliding along with other natural factors have various effects on A. adscendens plant form which can be grouped under three categories: direct mechanical effect of snow, physiological effect of snow, and indirect effect of precipitation and wildlife. The environmental factors and plant physiological responses to them change the A. adscendens plant form from a funnel or ob-conical shape to a semi-funnel or semi ob-conical form.

---

The main sources of cadmium in soil-plant continuum in amounts that might present a hazard are liquid and solid wastes of sewage sludge, farm manures and fertilizers. In the southwest of Iran (Khuzestan Province) over 50,000 ha of land is under sugarcane (Saccarum officinarum) cultivation and more than 80,000 ha will be under sugarcane by the end of the year 2000. In these sugarcane fields, about 400 kg ha^-1 diamonium phosphate (DAP) and 400 kg ha^-1 urea are applied annually. There is no data available to show the fertilizers impact on soil, water and plant contaminations in Iran with respect to cadmium. The objective of this research was to compare the extractable cadmium of virgin soils with that of soils under sugarcane.

 Four sugarcane growing stations viz. Haft-tapeh, Karoon, Shoeibieh and Ghazali with cultivation histories of 36, 20, 2 and 1 year, respectively, were selected. In each site, along a transect soil samples from 0-30 cm of both furrows and ridges of cultivated soils and of virgin soils were collected. Electrical conductivity (EC), pH, clay and organic carbon contents, CI and Cd of 101 soil samples were measured according to standard methods. Results showed that increasing either EC or CI increased Cd concentration with its maximum in virgin soils and its minimum in furrows.

 Results also indicated a slight decrease in the Cd content of cultivated soils.

---

In order to evaluate the morphological and agronomic characteristics of 19 land races of onions and one foreign cultivar (Yellow Sweet Spanish), an experiment was conducted in 1998 at the Research Farm of the College of Agriculture, Isfahan University of Technology.

 Analysis of variance showed significant differences among genotypes for all of the morphological and agronomic traits. Dry weight and number of days to emergence had the highest and lowest coefficients of genetic variability, respectively. Plant fresh weight, yield of 30 plants and total yield also had high coefficients of variability. Broad sense heritability estimates were high for all of the traits, indicating low environmental affects them. Based on cluster analysis and plot of the first two canonical variables, the genotypes were classified in four groups with different agronomic traits. Canonical discriminant analysis based on nine agronomic traits introduced three canonical variables which justified 99.9 percent of the total variation among characters. Principal component analysis revealed four components while factor analysis showed three factors which explained 87.3 and 95.5 percent of the total variation among characters, respectively. The first and second factors were related to adaptation and assimilate translocation, respectively. Bulb diameter, bulb height and bulb weight in positive directions and sensitivity to Fusarium in negative direction had greater loads in the third factor.

---

Field studies of S. cretica Led. damage and potential crop losses on two important cultivars of sugarcane, NCo 310 and CP 57-614, were carried out in southern Khuzestan in 1998-1999.

 CP 57-614 showed greater mean percentage of bored nodes and internodes (20.7%) than NCo 310 (11-2%). Apart from the direct losses in cane weight (cane yield) due to boring from the larvae, cane juice quality is also adversely affected, resulting in lower recovery of sucrose in the factory. Sugar per ton of stalks also was greater in NCo 310 (132 kg) than in CP 57-614 (110 kg). Estimated sugar losses were 0.11 tons ha-1 for every 1% bored and rotten stalks. Other indirect losses are also discussed.

---

Pathogenicity and virulence of entomogenous fungus, Verticillium lecanii, was studied on the pea aphid, Acyrthosiphon pisum. Vertalec, a commercial product of V. lecanii, was evaluated under controlled conditions. Second instar nymphs were inoculated with conidial suspensions at concentrations ranging from 10⁴ to 10⁸ conidia/ml, and sterile distilled water (containing wetting agent) as control, then caged on cutted alfalfa stems. Each concentration was replicated three times with 30 aphids treated per replicate. Temperature, relative humidity and photoperiod were maintained at 23±1 °C, %97±3, and 16:8 (L:D), respectively. Aphids were monitored daily over 12 days for mortality and nymph production. All newborn nymphs and dead aphids were removed daily.

Vertalec significantly increased aphid mortality (mean mortality because of mycosis increased form %45.55±6.93 at 10⁴ conidia/ml to %95.55±4.45 at 10⁸ conidia/ml). The LC₅₀ value for pathogen was 5.l4×l0⁴ conidia/ml. LT₅₀ values for 10⁵, 10⁶, 10⁷ and 10⁸ conidia/ml were 10, 8, 6.5 and 5 days, respectively. At 10⁴ conidia/ml LT₅₀ value was not determined at experimental period. R_o values significantly decreased with increasing conidial concentration (mean R₀ altered from 28.l5±5.38 in control to 5.15±1.81 at 10⁸ conidia/ml). Results indicated that vertalec can be an effective agent against pea aphids. Further studies are recommended for its evaluation under natural conditions.

---

The effect of different media including the growth regulators on somatic embryogenesis from Mexican lime was studied. The average embryo formation after 60 days from unfertilized ovules in different media was evaluated at 0 to 33.75%. The most effective hormone to stimulate embryogenesis was GA₃ at 0.01 and 0.1 mg/l concentrations. Malt extract (ME) at 300 mg/l increased embryo formation, whereas at 1000 mg/l, it inhibited embryogenesis but produced embryogenic callus. Banzyl adenine (BA) at 0.1 and 0.01 mg/l concentrations had significant effects on embryogenesis, while embryo formation was inhibited at high concentrations. Embryo development in different media was also studied. The index of mean root length to mean stem length in the MT³ medium supplemented with 0.1 mg/l of GA₃ was observed to be 1.22±0.22, which was regarded a suitable medium for embryo formation, embryo development and plantlet regeneration.

---

A number of halophytic species as Salicornia europea, Halocnemum strobilaceum, Aeluropus lagopoides, and Aeluropus littoralis were found to occupy a significant portion of the total vegetation of the surrounding area at the Zayande-Roud inlet to Gavkhoony wetland. However, their ecological demands and bioenvironmental factors by which vegetation community composition has been affected is not undestood. A compehensive knowledge of the establishment is essential for future improvements in using the above species on salanized regions. In this paper, using the ordination method. The establishment pattern of  these four species in a range of varied habitats is evaluated based on the recongition of the relative  significance of habitat soil chemical properties and vegetion crown cover to the establishment of the four species. For this purpose, 48 plants meansurements were taken along a transect, having more species variation in term of vegetation cover percentage. Furthermore, 48 soil samples were taken from the plot along the same transect in a one-year period in 1999. The soil samples were analysed for PH, EC, available Na, K, Ca and Mg as well as clay, and silt contents. The ground and field vegetation data were analysed using the Principlas Components Analysis (PCA), and Canonical Correspondence Analysis (CCA) to produce summary vectors (PCA axes) of both the soil chemistry and habitat vegetation structure datasets. The summary of ordination method quantified the degree to which soil variables and species cover were related to variability in ground vegetation composition. variation in community composition (type and percentage) was significantly related to gradient of the aforementioned soil factors. Generally, the vegatation community composition in this experiment could be considered as a key component to expand the growth and development patterns of these species to similar salinised regions. 

---

Soil organic matter and clay minerals adsorb and immobilize extracellular enzymes of microorganisms and increase soil enzymes stability. This study aims to clarify the relative importance of soil organic matter and clay minerals on the cellulolytic activities of soils. Fluca prepared cellulase was immobilized on some agricultural residues and clay minerals, avicel and a sample of soil. Immobilized exoglucanase and endoglucanase were assayed at different times. Activities of the immobilized enzymes were strikingly dependent on the kind of sorbent. After 20 days of storing in refrigerator at 4^oC, the deactivation of immobilized enzymes on the organic substances (as avicel) was very low but deactivation of immobilized enzymes on the soil and clay minerals was relatively high. On the other hand, the activities of immobilized enzymes on the agricultural residues and avicel were significantly higher than the soil and clay minerals. So it may be concluded that a large part of cellulolytic activity of soil is related to immobilized enzymes on agricultural residues. Coating of the clay minerals, soil and avicel with 4 mmol of Al (OH) x per mg of them significantly increased their immobilization capacity and activities of immobilized enzymes. Activities of immobilized exoglucanase and endoglucanase on the Ca- homoionized soil and clay minerals were significantly higher than the K- homoionized soil and clay minerals. However, these effects may be related to the specific effects of cations on the method of enzyme assay or enzymes activities. These homoionizing-cations effects on the activity of immobilized enzymes on avicel were not significant.

---

Rice husk, an agricultural waste, is produced about 100 million tons annually in the world and 0.5 million tons in Iran. Due to growing environmental concerns about disposal of these wastes, efforts are required to apply the wastes in industry. In this research, the mechanical properties of concrete incorporating rice husk ash (RHA) in sulfate environments (such as irrigation canals) were investigated and the increase in concrete strength was compared with control samples. In order to burn the husks at a controlled temperature to obtain a highly reactive pozzolanic RHA, a furnace was designed and built. The experiments included 405 samples of cubical (707070-mm) and cylindrical (50.8101.6-mm) concrete samples (105 samples for A, B and C treatments, respectively) which were stored in different ages (7, 28, 60, 180 days) under three different conditions (solutions of magnesium, calcium and sodium sulfates). The portions of RHA as cement replacement were 20 and 30 percents for B and C treatments. The results showed that the samples of concrete containing 20 percent RHA as cement replacement had higher compressive and tensile strengths in sulfate environments at 180 days compared with those of control concretes. The concrete samples containing RHA showed sharper gradients compared to control samples of up to 180 days under sulfate conditions. The best portion of RHA in concrete was determined to be 20 percent by weight.

---

Experiments were carried out in order to micropropagate sugarcane cultivars through shoot tip and auxilliary bud culture. Rinsing of four cultivars of sugarcane, namely CP-48-103, CP-57-614, CP-69-1062, and NCO-310 in 75% alcohol for 60 seconds and their subsequent disinfection with sodium and calcium hypochloride (1.5% active material) for 15 minutes decreased a significant amount of infection of explants in the medium. The use of the Murashing and Skoog (MS) solid and liquid medium with 1 mg/l Indole Butyric Acid (IBA), 1 mg/l Kinetin, 100 mg/l mio-inositol, 1 mg/l Thiamin HCl, and 2% sucrose had significant superiority (P<0.05) to 1/2 MS solid medium. Also, to increase the multiplication in a sterile medium (In vitro), two kinds of solid and liquid MS medium, with a hormone combination of 1 mg/l IBA, 2 mg/l Kinetin and 1 mg/l 6-(benzylamino)-9-(2-tetrahydropyranyl)-9H-purine (BAP) were applied which yielded the highest amount of proliferation. The plants formed roots in Schenk and Hildebrandt (SH) medium with a hormone combination of 5 mg/l IBA and 1 mg/l Kinetin. When activate charcoal was used in the medium, a higher percentage of the plants became rooted and a larger number of adventitious roots were produced than in the dark-light or light treatments.

---

Canning of sausage is one of the methods to extend the shelf life of sausage with lower percentage of additives such as nitrite. This method reduces transport and storgae costs, too. The present stduy was carried out in two stages. The first stage consists of preparing sausages with four different formulations in Isfahan Senobar Factory and producing canned sausage. In the second stage, physicochemical and organoleptic experiments were carried out. Canned (214409) and bottled (No. 10-67) sausages were produced and sterilized in autoclave (absolute pressure=2 atm) to give F value = 4 min. Cans and jars were filled with skinless sausage of 22 calibre and brine (2% and 25^oC) at a sausage/brine ratio of 1:1. Chemical composition such as moisture, fat and protein were measured according to the standard and AOAC methods. Physical experimets including measurement of texture shear strength were performed in sausage and canned sausage over the 3-month storage period. Factorial experiments in completely randomized design was used to investigate and analyze statistical data in this study. The Duncan’s multiple range test was used to compare data averages. Moisture content increased and sausgae texture shear strength decreased in all formulations as a result of sterilization. Moisture content increased in four canned sausage formulations while protein and fat decreased (by the end of the second month of storage). Also, sausage texture shear strength was observed at this time to decrease. In organoleptic evaluations, four canned sausage formulations were not significantly different in flavor, texture, and color (P<0.01).

---

Mayonnaise is a food emulsion containing egg yolk as emulsifier and some sort of gum as stabilizer. Iran is a main producer of tragacanth gum in the world which could be a potential replacement for imported gums used in making mayonnaise .The objective of this study was to investigate the possibility of substituting this gum for imported stabilizers in the formulation of mayonnaise sauce. To do this, tragacanth powder was added to the sauce at the levels of 0.1, 0.2, 0.4, 0.6, 0.8 and 1% . Emulsion stability and apparent viscosity of the sauces were determined and compared with that of the commercial one to find the right concentration of tragacanth. This concentration was used to produce mayonnaise on a commercial scale and then its physico-chemical characteristics, microbiological stability, and organoleptic properties were compared with commercial mayonnaise. The shelf-life of the sauce samples were also monitored bimonthly up to 4 months at two different temperatures (5 and 25 ^oC) by measuring apparent viscosity, moisture content, pH, microbial count, and organoleptic parameters. The results were compared with those obtained for commercial sauce. The results indicated that the characteristics of tragacanth-containing mayonnaise were similar to those of the commercial one and that the former was acceptable to consumers. Therefore, gum tragacanth at the right concentration could be a suitable subsitute for the stabilizers and thickeners already used in making mayonnaise sauce.

---

In this study changes in microstructure of casein micelles in raw and pasteurized milk were investigated by SEM and TEM. Milk was pasteurized by L.T.L.T. method (Temperature: 63 ^oC_ Time:30 minute).Samples of raw and pasteurized milk were taken from research pilot plant of The College of Agriculture. Each sample was divided into two parts. One part of each sample was directly prepared for SEM and TEM, whereas the second part were renneted at 2 ^oC and kept at this temperature for 24 h. and then prepared for evaluation by electron microscopes. Results indicated that in native state, particularly in raw milk casein micelles are in spherical shape with smooth surface and in the single form. The samples, which stored in the cold were under the effect of the rennet, encounter of filamentous status that might be due to the changes occurred in kappa-casein following the action of rennet along with partial removal of beta-casein and micellar calcium phosphate. Micrographs of casein micelles in pasteurized milk indicated that thermal condition during pasteurization had influenced on increasing of casein micelles size.

---

Estimation of seepage is essential prior to lining of earth canals. In Iran such investigation has been achieved in some irrigation networks using empirical relationships derived in other countries. Estimation of water loss in canal is required in design, operation and management of water distribution systems. Water seepage may be determind by using empirical equations proposed by F.A.O. These equations are applicable for different soils and hydraulic parameters. However, the appropriate estimating equation should be determined for each region. Therefore, these equations should be calibrated for local usage and different canal vegetation conditions. In this investigation water losses in canals at the Rudast region of Isfahan were measured by inflow and outflow procedure. Different canals reaches were selected in soils of relatively heavy, medium and light textures. The density of vegetation population in canals were low, medium and high. The estimated seepage losses by different empirical equations were not corresponded to those of measured values. Therefore, by using the measured seepage at different soil textures and vegetation densities the empirical coefficients of six empirical equations of F.A.O. (Ingham, Davis and Wilson, Affengendon, Moritz, Molesworth and Yennidumia, Misra) were modified for the study region. The relationships between measured seepage and estimated seepage before and after modification of the empirical equations were determined by regression analysis. These equations estimated the seepage loss much smaller than the measured values. The regression parameters (selope, intercept, and coefficient of determination of regression equation) indicated that after modification, the Ingham and moritz equation with higher slopes (0.91, 1.01), lower intercepts (-0.096, -0.039) and higher coefficient of determination (0.96) estimated the closest seepage values to the measured values respectively. The misra equation was the next best equation for seepage estimation. The results of present investigation indicated that the modified Ingham and Moritz equations were the most appropriate ones for estimation of seepage losses at different soil textures and vegetation densities in the study region.

---

Canola (Brassica napus) is one of the important oil seed crops cultivated in many parts of Iran. One of the main problems of seed production of canola is pollination and fertilization of flowers. Insects are the major pollinators. In order to study the fauna of insect pollinators of 3 cultivars of canola including Talaye, Okapi and S.L.M. and the difference between the attractiveness of these cultivars to insects during the flowering period, insects were collected by sweeping net and identified. Their abundance was determined at different periods of time during a day. Results showed that insect pollinators belonged to five orders and 18 families. The most abundant pollinators were Hymenopterous insects specialy honey bees (Apis mellifera) which were about 50%. Irrespective of Apis mellifera, the most abundant insects captured belonged to Diptera. Three Dipterous insects, Bibio hortulanus, Metasyrphus corollae and Platypalpus sp. were collected and identified. During the flowering period (March to mid April) in Isfahan, honey bees were more abundant in the warmer hours of the day, mostly noon and afternoon, but were less active in the early hours of the day, morning, because of cold weather and low nectar production. Honey bees were more active than other pollinators on Talaye at 9 a.m. and on Okapi at mid-day, noon, and during afternoon. Results also showed that insect pollinators could increase seed yield by 53%.

---

Cultivation of a second crop in paddy fields of Guilan province is a strategy for optimum use of land, supporting family economy and increasing oil-seed production. However, this is impeded under high rainfall and waterlogging conditions. To enhance second-crop cultivation in paddy fields, surface drainage is inevitable. The surface drainage practices should be performed with minimum costs, least time span and least variations of the land. In the present research, longitudinal surface drains were constructed with a spacing of 2, 4, 6, 8 and 10 m (depth of 20 cm), with and without 1-m lateral drains (depth of 10 cm) in Rice Research Institute, Rasht, Iran. The experiment was conducted in a complete randomized block design with three replications. Canola (Brassica napa), variety PF, was cultivated as a second crop after rice. The results showed that the effects of drain spacing on seed yield, days to maturity, number of plants per m², and also the effects of lateral drainage on seed yield, plant height, residual N, days to maturity, and number of plants per m² are significant (P<0.01). Highest yield (2493 kg/ha) was obtained from longitudinal drains 4 m apart, with lateral drainage drain spacing of 6 and 2 m had acceptable yields, too (2241 and 1817 kg/ha, respectively). For treatments without lateral drainage, 2-m drain spacing had the highest yield (1324 kg/ha). Considering all the expenses for drainage construction, it was found that net benefit of longitudinal drain spacing of 4 and 6 m was twice the costs. Since the rainfall in Nov., Dec., and Jan., 2000, was higher than the 20-year average rainfall of the same months, it was concluded that the results of this experiment may be recommended in the years with a rainfall less than the year 2000.

---

Poor performance of irrigation canals and its effect on decreasing of Agricultural water productivity require attention for their improvement. In this paper a new mathematical model is introduced which could present optimal operation considering downstream requirements of turnouts, canal inlet flow, actual constraints and real conditions of canal system. Four performance indicators of delivery including efficiency, adequacy, equity and stability are considered as an objective function in the process of optimization. Since this objective function is an implicit function of decision variables (regulation of turnouts and control structures) and hydraulic parameters, it is necessary to implement hydrodynamic model, using numerical optimization methods. SA (Simulated Annealing) technique is a numerical meta – heuristic intelligent search method which is used in combination with a hydrodynamic model (ICSS) (Irrigation Conveyance System Simulation.) for performance optimization of canal system. Theoretically it is proven that SA technique is capable of tending towards global optimum solution asymtotically. Taking short random steps in SA algorithm guarantees avoiding instability in hydrodynamic model. The developed model has been applied on E1R1 Distributary canal of Dez irrigation network for ten days. The results indicated that optimal performance improved very well in comparison with the present situation.In this model the weighting coefficients of indicators are determined using sensitivity analysis in optimization process. Consistency test on the derived coefficients shows that proposed method is appropriate. Applying weighting coefficients for performance indicators in the processes of optimization has resulted in 7 to 21 percent improvement compared to the case of equall weighting coefficients. Also, the results indicate that the developed model (ICSS-DOM) (ICSS-Delivery Optimization Model) is an efficient tool for the evaluation and optimization of irrigation canal performance, producing good and valid results in a relatively short and suitable time.

---

Pollination and fertilization of canola flowers are the main factors of canola seed production. In order to study the effects of insect pollinators on seed quantity and quality of three chosen cultivars of canola, namly, S.L.M., Okapi and Talaye, a split plot experiment in a complete randomized block design with four replications was carried out in Isfahan region. Main plots consisted of canola cultivars, and subplots consisted of open pollination and non-pollination by insects. Results indicated that the time to seed ripening in pollinated plots was 10.5 days less than those of non-pollinated plots. Pollination by insects increased the number of pods per plant, number of seeds per pod and weight of 1000 seeds, by 14.3%, 23.5% and 10%, respectively. Grain yield also increased by 53% in pollinated plots. Pollination decreased seed moisture percentage by 3.15%, but its effect on seed oil percentage and germination percentage were not significant. Time to seed germination of plants of pollinated plots decreased three hours. Cultivars were significantly different in seed oil percentage and time to seed germination. Talaye cultivar produced the highest amount of oil (45.5%), while Okapi produced the lowest (40.8%). There were significant interactions between cultivars and pollination in terms of seed ripening, oil percentage and moisture of seeds, and the effects of pollination was different in different cultivars.

---

Sugarcane (Saccharum officinarum L.)‌ and rotational crops have been cultivated without potassium fertilizers for many years in southwestern Iran. Although potassium was removed from this soil, no response has been reported to K fertilizers by crops. This study was conducted to evaluate the effects of wetting and drying, and cultivation systems on potassium fixation in some Khouzestan soils. The results showed that expandable clay mineral was observed in cultivated soils but not in the non-cultivated soils. This may be related to irrigation, and cultivation practices in this soils. By adding K and sequential wetting and drying the amount of K-available increased in sugarcane, rotational cropping and uncultivated soils from 132.6, 226.2 and, 171.6 mgkg^-1 to 266, 447 and 628, respectively. These results showed that more K can be fixed after cultivating soils, especially by sugarcane, but available K increased by adding K. All surface soils had higher K fixation capacity than subsurface ones. Also, by application K to these soils, the amount of K fixation decreased with increasing wetting and drying times. The K fixation increased by the increase of cation exchangeable capacity. Significant difference was observed between cultivation system and depth of sampling in 1% levels. High K fixation can be attributed to illite minerals depleted from K. Mica-like minerals formed after adding K, and wetting and drying cycles. Amount of K fixation by clay particle samples was more than the same soil samples but in both the same trend was observed for K fixation. Also, drying and wetting decreased K fixation in the last periods. It may be due to trapping K in the interlayer positions.