JWSS - Journal of Water and Soil Science

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Whole corn plant was ensiled in 70 gr minisilos for 60 days to evaluate the effects of addition of two levels of Cellulomonas uda bacteria (0, 1.2×10⁵ and 2.4×10⁵ cfu per gr of fresh forage) on fermentation and chemical composition of silage. The experiment was arranged as a completely randomized design. The silos were opened after 3 and 6 days of fermentation. The criteria measured included dry matter (DM), pH, organic matter, water soluble carbohydrates, crude protein, cell wall (NDF), acid detergent fiber (ADF), hemicellulose, cellulose, and ammonia-N contents.

Both levels of bacteria significantly decreased the pH of silages after 3 days (P<0.05). The final pH of silage with the higher level of bacteria was significantly lower, indicative of a greater fermentation of cellulose in this silage. Addition of bacteria resulted in a non-significant increase in the level of final residual water soluble carbohydrates. Final silage ammonia-N concentration was less than 0.8% of dry matter in all silages and was not affected by bacterial treatment. The ADF content was decreased by 11.9% in the silage containing the lower level of bacteria and by 1.8% in the silage which contained the higher level of bacteria. The NDF content increased in the control silage and that with the lower level of bacteria but decreased by 3.6 gr Kg DM^-1 in the silage with the higher bacterial level. Hemicellulose contents increased in all silages but cellulose contents decreased by 9.38% (control), 17.37% (low bacteria) and 12.49% (high bacteria) in the control and bacteria-treated silages, respectively. The results indicated that the addition of 12×10⁵ cfu Cellulomonas uda per gr of whole fresh corn plant was effective in increasing residual water soluble carbohydrates, reducing NDF and hydrolyzing cellulose of whole corn plant.

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The objective of this study was to evaluate the characteristics of millet silage prepared in laboratorial mini silos and to determine the effects of additives on its fermentation quality. Whole millet (Panicum milliaceum) was harvested at milk and soft-dough stages of maturity, left untreated or treated with ground barely, molasses, formic acid, lactic acid producing bacteria, and combination of molasses plus inoculant, and were ensiled in a completely randomized design in 2×6 factorial arrangement. Stage of maturity had no significant effect on NDF and ADF, buffering capacity, residual water soluble carbohydrate, acid detergent insoluble nitrogen, ammonia nitrogen and in vitro dry and organic matter digestibility however, values for dry matter, crude protein and pH were significantly different. Loss of dry matter, elevated NH₃-N (24% of total nitrogen) and increased butyric and acetic acid levels (.2 and 1.33% DM, respectively) in control silage indicated clostridial fermentation. Between treatments, the lowest NH₃-N was observed for silages treated with formic acid. Molasses and molasses plus inoculant produced higher lactic acid and lower butyric acid. Higher in vitro dry matter and organic matter digestibility showed positive effects of additive usage compared to control group. Addition of molasses and molasses plus inoculant resulted in higher in vitro dry matter digestibility (63.1 and 64.3 %, respectively), but no significant differences were observed between barely, formic acid and inoculant treated silages. Results of this study clearly showed the need for adding a source of water soluble carbohydrates to millet in order to obtain a good quality silage. Also inoculation of lactic acid bacteria does not necessarily promote homolactic fermentation if insufficient amounts of water soluble carbohydrates are presented.

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Eight hundred and fifty two multiparous Holstein cows in mid-lactation were used in a completely randomized and nested design with two treatments and three replications. Treatments were: 1) Isfahan and 2) Borkhar-Meymeh. Replications were: 1) class of one to five cows 2) class of six to ten cows and 3) class of eleven to twenty cows. Paired t-test was used for the comparison of nutrient consumption and requirement. The obtained results of one-year-old experiment indicated that township and different classes had no effect on consumption of dry matter intake (DMI), net energy lactating (NEL), metabolizable protein (MP), calcium (Ca), phosphorus (P), potassium (K), magnesium (Mg), sodium (Na), chlorine (Cl), sulphur (S), selenium (Se), zinc (Zn), copper (Cu), iodine (I), vitamin A, vitamin D, vitamin E, rumen degradable protein (RDP), neutral detergent fiber (NDF), acid detergent fiber (ADF) and non-fiber carbohydrate (NFC). Township had a significant effect on consumption of rumen undegradable protein (RUP) (P<0/05). Township and different classes had no effect on nutrient requirements of dairy cattle. The difference between consumption and requirement for kg DMI, Mcal/d NEL, g Mp, (g absorbable) Ca, P, K, Mg, Na, Cl, S, mg per kg Se, Zn, Cu, I, Iu/d vitamin A, vitamin D, vitamin E, g/d RDP, RUP, percentage of dry matter NDF, ADF and NFC were -1/6, -2/5, -630/2, -21/8, -5, 40/6, 0, -3/3, 44/2, -7/4, -3/11, -38/7, -3/26, -4/45, -14834, -234, -342/5, -542/5, -886, 28/5, 17/5 and -10/4, respectively. The difference between consumption and requirement was not significant for P, Mg and Na. This difference had a significant effect on the rest of nutrients (P<0/05). The results of this study indicated that we can feed dairy cattle by using diets containing protein supplements and mineral-vitamin premix and improve milk yield and percentage of milk protein's rural areas of Holstein cows.

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Nowadays, Inulin, due to its nutritional properties, is widely being used all over the world. Artichoke tuber is one of its major industrial extraction sources. Therefore, in the present study we initially aimed to determine total carbohydrate, inulin, reducing sugars as well as dry matter contents of an Iranian artichoke. Thereafter, we focused on the optimization of aqueous extraction of inulin in the presence and absence of direct and indirect ultrasonic waves at various combinations of extraction time, temperature and solvent:solid ratio using Box-Behnken design and response surface methodology (RSM). Based on the experiments, the dry matter and total carbohydrate contents of Iranian artichoke were 6.7 and 3.9%, respectively. We also found that an empirical model developed by RSM could satisfactorily describe relationship between independent parameters and the yield of total carbohydrate as well as inulin. Furthermore, our findings revealed that optimal aqueous extraction of inulin could be achieved at 80ºC, for a duration of 5 min at a solvent:solid ratio of 1:5. Moreover, a direct relation was seen between the yield of extraction and the power as well as frequency of ultrasonic waves where with increasing latter variables, duration of extraction process and inulin yield considerably reduced and increased, respectively.

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Determination of carbohydrates in soil requires prior extraction and numerous extraction methods were suggested for this purpose. Three methods and five extractants were applied in order to extract carbohydrate fraction in three soil types forest, clayey and saline soils. The extraction methods were: 1) shaken in a plane rotary shaking machine for 16 h 2) heated in steam-bath for 2.5 h and 3) heated in oven for 24 h and extractants included 1) 0.5 M HCL, (2) 0.25 M H2SO4, 3) 0.5 M H2SO4, 4) 0.5 M K2SO4 and 5) distilled water. Carbohydrate content in soil was measured by phenol-sulphuric acid method. The addition of phenol to the extracted solution of HCl caused to milky precipitation. Therefore, this extractant can not be used for carbohydrate extraction in the phenol-sulphuric acid spectroscopic method. The results showed that in all soils and in the shaker extraction method, carbohydrate content was lower than in the oven and steam-bath extraction methods. In the forest and saline soils, the extracted carbohydrate content was not significantly different among the oven and steam-bath methods. For the clayey soil, the carbohydrate content was higher in the oven method than that of the steam-bath method. In all soils, the extracted carbohydrate by 0.5 M H2SO4 extractant was greater than those of other extractants. In the forest and saline soils, the extracted carbohydrate by distilled water was lower than those of other extractants. Using steam-bath method (for the forest and saline soils) and oven method (for the clayey soil) with 0.5 M H2SO4 extracted the highest amount of carbohydrates.

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In order to assess the effect of drought stress and various levels of potassium on solutes accumulation and chlorophyll of canola and Indian mustard, a field experiment was conducted in a factorial design based on randomized complete block design with three replications including three irrigation regimes (I1=irrigation after 50% depletion of soil water(control),I2 =irrigation after 70% water depletion and I3 =irrigation after 90% water depletion), two species (Hyola 401 hybrid of canola and landrace cultivar of mustard) and three levels of potassium fertilizer (K1=0 ,K2=150 and K3= 250 kg.ha-1 K2SO4 ) at Agricultural and Natural Resources Research Center of Sistan in 2008-2009 cropping season. Water stress increased proline and soluble carbohydrate accumulation in the leaves of Brassica sp. In non stressed condition (control) in different growth stages, proline was lower than water-stressed plants and Leaf proline content decreased significantly after irrigation. Mustard landrace showed higher capability for accumulating assimilates such as proline, soluble carbohydrates and potassium than hybrid Hyola 401. Water stress decreased the amount of chlorophyll a, b and total leaf chlorophyll, but Potassium application caused an increase in the mentioned parameters. The highest content of chlorophyll pigments was observed at flowering stage. Potassium application caused a decrease in proline and an increase in soluble carbohydrates concentration in the leaf under water stress condition. There was a negative correlation between grain yield and proline content and soluble carbohydrates, but grain yield was positively correlated with chlorophyll, a, b. It was concluded that osmotic adjustment can be an important mechanism for Brassica species under water stress conditions and that organic and inorganic compounds such as proline, soluble carbohydrates and potassium play key roles in this regard.

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Arbuscular mycorrhizal fungi (AMF) are widespread endosymbionts in terrestrial ecosystems and their exudates have important effects on soil properties. A greenhouse experiment was conducted with six AMF treatments including four exotic species inoculums (Funneliformis mosseae ,Claroideoglomus claroideum  and Rhizophagus irregularis and a mixed isolate of three species), one mixed native AMF species treatment and a sterilized soil (control) with four salinity levels (1, 5, 10 and 15 dS m^-1). AMF increased the soil (EEG) and total (TG) extractable glomalin, and also the hot water (HWC) and diluted acid (DAC) extractable carbohydrates compared to control treatment in all salinity levels. The native AMF species had the greatest effects on EEG, TG, HWC and DAC at 10 and 15 dS m^-1. Soil EEG and TG concentrations were higher in the mixed exotic AMF treatment than in each AMF species. The greatest glomalin concentration was related to F. mosseae at 1, 5 and 15 dS m^-1 but at 10 dS m^-1 the greatest glomalin concentration was related to C. claroideum. The greatest carbohydrate concentration was related to F. mosseae at 1 and 5 dS m^-1 but at 15 dS m^-1 significant differences were observed among the three AMF species. Our results showed that there is an interaction between salinity and different AMF species, and a combination of them determines the function of AMF.

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