Showing 3 results for Carnation
A. Deljou, O. Karami, M. Esna-Ashari,
Volume 11, Issue 1 (4-2007)
Abstract
In vitro regeneration of four cultivars of carnation namely ‘Nelson’, ‘Impulse’, ‘Sagres’ and ‘Spitit’ through somatic embryogenesis was studied. MS culture medium was supplemented with 30 gl-1 sucrose, 2 mgl-1 2,4-D and 0.2 mgl-1 BA and used for embryogenic callus formation. Somatic embryos were formed when embryogenic callus was transferred to MS medium without growth regulators containing 30 g/l-1 sucrose alone or supplemented with different concentrations of mannitol (15, 30, 60, 90, 120 and 150 gl-1). No somatic embryo was formed on culture media containing mannitol without sucrose. Number of somatic embryos produced from embryogenic calli significantly increased by adding mannitol to the culture media. Normal embryos formed on culture media containing high concentrations of mannitol (60, 90, 120 and 150 gl-1) developed normally. About 95% of somatic embryos transferred to the1/2 MS culture medium containing 30 gl-1 sucrose, germinated into plantlets. Plantlets also continued their growth under greenhouse conditions.
E. Karimi, H. Rouhani, D. Zafari1, Gh. Khodakaramian, M. Taghinasab,
Volume 11, Issue 41 (10-2007)
Abstract
In order to study the biological control of carnation vascular wilt disease caused by Fusarium oxysporum f.sp. dianthi, 141 bacterial strains were isolated from carnation rhizosphere, and their antagonistic activity was evaluated against fungal pathogen in dual culture method. Among the tested strains, 16 strains showed antagonistic activity seven of them with more activity were selected for further investigation. Based on phenotypic features, strains E31 and E57 were identified as Bacillus cereus E76, E93, E102 and E121 as Bacillus subtilis and E130 as Pseudomonas fluorescens bv. III. All bacterial strains inhibited mycelial growth of F. o. f. sp. dianthi by production of non-volatile and volatile metabolites under laboratory condition. Microscopical analysis showed that all strains caused deformation of pathogen mycelium, and metabolites of these strains reduced conidia production rate and as well as the ability of conidia germination. In the in vivo tests, in sterilized and nonsterilized soils, the effect of bacterial strains was studied on disease severity, percentage of healthy plants and the growth rate of plants using soil inoculating and root-dipping into bacteria-methyl cellulose mixture methods. The E57 and E121 strains, in both methods, and E130 in root-dipping method showed highest effect on decreasing of disease severity and increasing of healthy plants percentage. Strains E57, E121 and E130 significantly increased total dry weight of carnation. Maximum dry weight was obtained by E57 and E130 in soil inoculating and root –dipping methods respectively.
O. Karami, A. Deljou, A. Mahmoudi Pour,
Volume 12, Issue 43 (4-2008)
Abstract
In vitro regeneration of two cultivars of carnation, namely, ‘Nelson’ and ‘Impulse’ was studied through direct somatic embryogenesis. Somatic embryos were formed directly on petal explants cultured on Murashige and Skoog (MS) medium containing different concentrations (0.2, 0.5, 1, 2, 4, 6 mg 1-1) of picloram. Maximum embryogenesis was obtained with 1 and 2 mg/l picloram. Globular shaped embryos were developed into cotyledonary-shaped embryos when they were transferred to the growth regulator-free media containing different concentrations (2, 4, 6, 8, and 10 %) of sucrose. Increasing sucrose concentrations in the culture media enhanced somatic embryos development. Cotyledonary somatic embryos developed plantlets when they were transferred to the half-strength MS culture medium containing 3% sucrose. Plantlets also continued to grow under greenhouse conditions.
