Showing 2 results for Chemical Composition.
H. Alipour, A. Rezai, S. A. M. Meibodi, M. Taheri,
Volume 5, Issue 4 (1-2002)
Abstract
This experiment was conducted to study genetic variation for electrophoretic seed protein patterns and their relations with some seed characteristics such as protein and oil percentages, chemical compositions and 100-seed weight among 270 soybean (Glycine max L. Moench) genotypes.
Among different electrophoresis procedures examined, 10% and 4.5% concentrations of acrylamide for resolving and stacking gels, respectively, 13.5 mg/ml concentration of protein buffer extraction, injection of 10 microlitre sample injection into gel hollows, 2.5 miliamper and 2-hours staining period were determined as the best combination to achieve clear bands and good separation. Based on relative mobility on gel, 30 protein bands were observed, of which only 5 varied among genotypes. In general, 8 electrophoretic patterns were recognized. Cluster analysis based on qualitative evaluation of patterns grouped the genotypes in 8 clusters and classified different bands in three groups. Simple concordance (matching) coefficients between protein bands with relative mobilities of 2.5% and 49.5% were zero, which is an indication of their independent occurrence. Probably, these bands are coded with one gene that in dominant and recessive homozygous genotypes appear as a single band at different positions on the gel. Analysis of variance revealed significant differences (P<0.05) among protein patterns for protein and oil percentages. Correlation coefficients between variable protein bands and studied traits showed a positive and significant relation (P<0.05) of bands with relative mobility of 3.5 and 49.5 with protein and phosphorous contents of the seeds, respectively. Protein patterns having band with relative mobility of 49.5 had the highest phosphorous content. Black hilum color of seeds was related to protein band with relative mobility of 52.
E. Ebrahimi, J. Pourreza, S. V. Panamariov, A Kamali, A. Hosaini,
Volume 8, Issue 2 (7-2004)
Abstract
A 3×4 factorial experiment was conducted for 45 days to determine the effects of diets containing different levels of protein and fat on growth of fingerlings Beluga (Huso huso L.1758). Twelve different diets were formulated with 3 levels of protein (45,50 and 55) and 4 levels of oil including fish oil and sunflower oil (0.5 ,4 , 8 and 12). 1260 Beluga fingerlings with an initial body weight (IBW) of 2.2 gram, after adaptation to the experimental conditions, were selected and stocked randomly into 36 fiberglass tanks (100 water volume) at 35 fish per tank, and fed on experimental diets for 45 days. Two ways analysis of variance showed that the percentage of body weight increase (%BWI), specific growth rate (SGR),protein efficiency ratio (PER), food concumpsion ratio, and productive protein value (PPV) content were significantly (P<0.05) affected by different protein and fat levels. Fishes fed on diets No.3 (containing 45% protein and 14 % lipid) and No.8 (containing 50% protein and 17 % lipid) showed the highest increase in body weight percentage, the best specific growth rate, protein efficiency ratio, and lowest food concumption ratio. Besides, the highest productive protein value was observed in diet No.2 (45% protein and 10.5% lipid) and diet No.3 (45% protein and 14 % lipid).Comparison of chemical composition of examined fish corps indicated a significant difference in their protein and lipid contents (P<0.05). However, the composition of the diet, revealed no significant changes on the dry matter and ash content of the body. Based on the findings of the present study 45-50% dietary crude protein is recommended for the optimum growth of Beluga fingerling. Moreover, the rate of needed lipid for optimum growth of fingerling range is from 14 to17 percents.
