JWSS - Journal of Water and Soil Science

---

Knowledge of the effects of row spacings and seeding rates on dual-purpose forage and seed production of an annual legume crop is essential for its proper agronomical exploitation. In this study, the effects of various row spacings and seeding rates on dry matter and seed yield in berseem clover (Trifolium alexandrinum L.) were evaluated in two consecutive years. The experimental design was a split-plot with four row spacings as main plot treatments in a randomized complete block design with four replications, and five seeding rates were allocated to subplots.

Combined analysis of variance of the two-year data showed that both row spacings and seeding rates influenced dry matter yield (DM), seed yield, plant height, 1000-seed weight and harvest index. Years, as well as two- and three-factor interactions did not affect the seed yield whereas row spacing × seeding rate, and year × seeding rate significantly affected the DM. The mean comparisons indicated that row spacings of 30 and 45 cm produced the highest DM of 3912.2 and 3900 kg/ha 45 and 60 cm spacings showed the highest seed yield of 1045.4 and 1093.3 kg/ha, respectively. 24 and 20 kg/ha seeding rates ranked the best in giving 1244.7 and 1223 kg/ha seed yield, respectively. The seeding rate of 16 kg/ha in the first year and 20 kg/ha in the second year produced the highest DM yield with means of 3837.5 and 3875.3 kg/ha, respectively. Regarding the dual-purpose of forage and seed production in berseem clover, 20 kg/ha seeding rate at 45 cm row spacing can be recommended for Isfahan region and for other comparable environmental conditions.

---

Field crop landraces are valuable genetic sources. Twenty populations of Persian clover (Trifolium resupinatum L.) collected from different areas of Iran were used in this study. DNA extractions were carried out using minipreparation method with equal amount of leaves from 30 plants of each population. DNA samples from 20 clover populations were evaluated using semi-random (ISJ) markers. Ten primers out of 30 which used IT (intron-targeting) and ET (exon-targeting) primers produced repeatable bands. Cluster analysis was conducted using NTSYS software and UPGMA method based on Jaccard's similarity matrix. Primers totally produced 111 bands, of which 93 bands (%93) were polymorphic among clover genotypes. The greatest and least amplification fragments belonged to IT_15-31 and ET_18-4 primers, respectively. Average band number per primer was estimated 11.1 bands. Furthermore, IT primers produced more polymorphic DNA fragments with higher resolution. Based on cluster analysis and cutting dendrogram in 0.8 similarity coefficients, clover populations were divided into five groups in which Kazerun and Kermanshahi (1) individually formed a separate cluster. According to similarity matrix, the least similarity (%42) belonged to Alvijan and Kazerun and the highest similarity belonged to Chegeni and Haftchin Hamedan. Clustering based on semi-specific PCR method almost substantiated the grouping based on geographical origin. Considering the results, it is concluded that PCR-based semi-random marker technique can be used for genetic diversity study of Persian clover as well as discrimination of its cultivars.

---

Pathogens are the most important factors inducing postharvest losses on citrus fruit. Experiments were conducted as a CRD with 4 replications on sweet lime (Citrus limetta) and Valencia orange (Citrus sinensis) in 2003 and 2004. Treatments were pure essential oils of clove and thyme and in 25% ethanol solution at concentrations of 0.1, 0.3 and 0.5% and imazalil (2 ml/l). Results indicated that in the first year, pure essential oils of clove and thyme reduced the decay percentage of blue mold of Valencia orange fruit from 90 % (control) to 0 and 12.5 %, respectively. Pure essential oils of clove and thyme reduced the decay percentage of sweet lime in the first year of experiment from 95 % (control) to 0 and 6 %, respectively. In the second year, with the same treatments decay percentage of blue mold was reduced from 90 % (control) to 0. Solutions of 0.1, 0.3 and 0.5 % of clove and thyme oil extracts in 25 % ethanol solution reduced decay percentage of sweet lime fruits form 85 % (control) to 65, 41 and 26 % for thyme oil and to 60, 30 and 22.5 % for clove oil. Comparison of pure clove and thyme oil extract with imazalil showed that pure clove and thyme oil extracts were not significantly different with fungicide treatment. Treatments of clove and thyme oils in 25 % ethanol treatment were not as effective as fungicide.