Rasool Jalili Marandi,
Volume 1, Issue 2 (10-1997)
Abstract
In this study, an experiment was carried out to determine the effects of 6 culture media (sand, perlite, vermiculite, Sand + Perlite, Sand + Vermiculite, Vermiculite + Perlite) on the rooting of single-bud cuttings of seven grape cultivars (Rishbaba, Hosseiny, Dashgarah, Ghiziluzum, Teberzeh, Bidaneh Sefid, Sahebi). All the cuttings were prepared from one-year old branches. Statistical analyses of the data obtained from the examination of the roots revealed significant discrepancies in the rooting of different cultivars' cuttings, media and media-cultivar interactions in terms of rooting percentage, fresh weight of roots and the ratio of dry to fresh weight of roots. The highest rooting performance in terms of the above parameters was observed in Hosseiny cultivar while the lowest obtained in Teberzeh cultivar. Vermiculite and perlite + Vermiculite were found to be the most suitable media for rooting of cuttings. According to the media-cultivar interactions, high rooting of Hosseiny, Dashgarah, and Ghiziluzum was observed in vermiculite and high rooting of Rishbaba, Teberzeh, Bidaneh Sefid and Sahebi was obtained in perlite + vermiculite.
A.a. Ehsanpour, M. Jones,
Volume 4, Issue 3 (10-2000)
Abstract
Potato is a valuable plant as a source of nutrition and as an industrial product. The modification and breeding of potato plants are very difficult, or even impossible, via the conventional plant breeding programs. However, plant biotechnology and tissue culture are reliable methods which can produce plant material for gene transformation using Agrobacterium as a vector. In this study, one-, two-, and three-step methods of plant regeneration from stem culture of potato cv. Delaware were tested. Among these only the one-step procedure using TDZ, a synthetic cytokinin, was the best for rapid plant regeneration. In this culture medium, several shoot buds and shoots were regenerated from stem culture, while the other methods using a culture medium supplemented with BAP, NAA, zeatin, 2ip, IAA produced white and green callus. Morphology and chromosome number of all regenerated plants were similar to the original plants. The results showed that the regeneration system was suitable for cv. Delaware and that the culture conditions prevented genetic variation of the regenerated plants.
