Showing 13 results for Durum
Sayed Shahram Mirodjagh, Ahmad Arzani,
Volume 2, Issue 3 (10-1998)
Abstract
Immature embryo culture response of 28 durum wheat (Triticum turgidum var. durum) cultivars was studied using MS medium in Tissue Culture Laboratory of College of Agriculture, Isfahan University of Technology. Donor plants were grown in a recirculating hydroponic system and pots in a greenhouse. Regenerated immature embryos were recorded at 2, 4, 8 and 16 days after inoculation. Assessment of durum wheat cultivars for immature embryo culture was conducted by percentage and rate of regenerated embryo, using a split plot design arranged in completely randomized design with 5 replications. The analysis of variances showed that there were highly significant differences among cultivars for plant regeneration capacity. The highest potential and rate of embryo regeneration (two days after inoculation) were observed in “Shahivandi” which is a native cultivar of west Iran. “Awl1/Sbl4” with a mean of 48.5%, “Hagla” and “Ofen/Kil” with a mean of 80% have the poorest and the best mean of regeneration during the recording period, respectively. The high regeneration potential is important in view of reducing the time constrain and somaclonal variation in a breeding program.
Sayed Shahram Mirodjagh, Ahmad Arzani,
Volume 3, Issue 1 (4-1999)
Abstract
In vitro salt tolerance of 28 cultivars of durum wheat with native and exotic origins was investigated, using MS medium containing sodium chloride. The resulting calli were subjected to 8 salinity levels including 0, 0.3, 0.6, 0.9, 1.2, 1.5 and 1.8 percent NaCl (w/v). Donor plants of explant (immature embryo) were grown in a recirculating hydroponics and pots in a greenhouse as well as in the field. Assessment of calli was conducted after 0, 8 and 16 days after subculture of calli into the NaCl-contained medium. Callus growth rate, relative callus growth rate and percentages of callus necrosis were measured, using a 8 × 28 factorial experiment layout in a completely randomized design with 3 observations per treatment and 3 replications. Results of analysis of variances showed that among the criteria, relative growth rate of callus was the most reliable, while callus growth rate as not being quantitatively measured, was less devoted. Results of this study revealed that 'PI 40100' and 'Dipper-6' were superior genotypes for in vitro salt tolerance. The high relative callus growth rate and less callus necrosis of these cvs. may indicate their superior genetic potential for in vitro salinity tolerance.
M. Shahedi, B. Mansouri,
Volume 4, Issue 3 (10-2000)
Abstract
Macaroni is a food product generally considered as one of the paste products. These products are produced from semolina that is, in turn, produced through special processing of milled durum wheat. In order to examine the effect of α-amylase on macaroni quality, three cultivars, namely Yavarous, Zardak and Altar 84, were chosen from each of which some wheat sprouts were prepared and mixed with unsprouted samples to obtain three ranges of falling numbers of 500-1000, 300-350 and 150-200 seconds.
The results from this study specified that the active presence of α-amylase in the samples reduced cooking time and colouring of the macaroni, while small amounts of the enzyme improved the breakage and modulus of elasticity of the samples. The α-amylase enzyme showed no effect on the colour of semolina and the macaroni stickiness.
M. Golabadi, A. Arzani,
Volume 6, Issue 3 (10-2002)
Abstract
To study grain quality traits and their relationships with high molecular weight (HMW) and low moleculor weight (LMW) glutenin subunits, 104 durum wheat genotypes were used. Six grain quality characteristics comprising wet and dry gluten content, test weight, grain hardiness, protein content and SDS sedimentation volume were studied. HMW and LMW glutenin subunits were evaluated using SDS-polyacrylamid gel electrophoresis (SDS-PAGE) in 33 genotypes. Statistical analysis including correlation coefficients, factor analysis, cluster analysis of genotypes based on qualitative traits, analysis of variance in qualitative traits based on HMW, LMW, and combination of subunits as well as all of subunits, and canonical correlation analysis between glutenin subunits and qualitative traits were used.
Factor analysis of the genotypes detected 2 factors, which explained 65 percent of the total variation among the data. These were named quantitative protein and qualitative protein. Based on cluster analysis, the genotypes were classified into four clusters. The genotypes in groups 2 and 4 were beneficial in terms of protein quantity and quality. In evaluating glutenin subunits, 7 HMW subunits and 2 LMW subunits were detected. Subunit null of Glu-Al was observed. Subunits 7+8, 6+8, 13+16 and 20 at Glu-Bl were observed however, neither of them had significant differences in qualitative traits, but 6+8 and 7+8 had a higher SDS sedimentation rate than 20. LMW-1 and LMW-2 had significant differences in protein content and SDS sedimentation LMW-1 had higher protein content whereas LMW-2 had higher SDS sedimentation. Combibation of subunit LMW-1 / HMW 7+8 had the highest protein content and LMW-2/HMW 7+8 had the highest SDS sedimentation. Results of canonical correlation showed that presence of LMW-2 and HMW 7+8, and absence of LMW-1 and HMW 20 in durum wheats caused an increase in SDS sedimentation and a reduction in protein and gluten content.
M. Golabadi, A. Arzani,
Volume 7, Issue 1 (4-2003)
Abstract
Genetic variation of 300 genotypes of durum wheat comprising CIMMYT/ICARDA and Iranian germplasm was evaluated in 1999-2000 at Researh Farm of College of Agriculture, Isfahan University of Technology located at Lavark, NaJaf-Abad. Days to heading, days to maturity, plant height, spike length, grain weight per spike, number of grain per spike, number of spikes per m2, 1000-grain weight, test weight, grain yield, biological yield, and harvest index were recorded. Coefficients of correlation, step-wise regression, factor analysis and cluster analysis of characters and genotypes were conducted using the studied traits. Results indicated that considerable genetic variations exist for the traits and, in particular for grain yield, harvest index, number of spikes per unit area and number of grain per spike. Grain yield had a positive and significant correlation with days to heading, days to maturity, number of grain per spike and grain weight per spike. Factor analysis for the genotypes detected 6 factors, which explained 76.7 percent of the total variation among data. These factors basically involved potential of assimilate distribution, different aspects of plant storage, source-sink relationships, plant height and tillering potential. Cluster analysis was similar to factor analysis in grouping the characters. According to cluster analysis, the genotypes were calssified into 6 clusters with significant differences among all groups. Mean comparisons of traits in these groups showed that genotypes of groups 5 and 6 were superior in grain yield and harvest index and are beneficial to the local durum breeding objectives.
T. Najafi Mirak, A. Zali, A. Hossainzadeh, Gh. R. Rasoulian, A. Saeidi,
Volume 7, Issue 4 (1-2004)
Abstract
Russian Wheat Aphid (RWA) is a serious pest of wheat, barely and other small grains in cereal-producing regions of the world. The use of resistant cultivars is an effective management strategy to control this pest. In this study, 15 lines and 13 cultivars of durum and bread wheats along with two resistant and susceptible controls were used to evaluate their reaction to RWA under greenhouse conditions, using completely randomized design with four replications. Three seedlings at each plot were considered as an experimental unit. Five aphid nymphs (4-5 ages) were placed on each plant at 1-2 leaf stage. Leaf rolling and leaf chlorosis percentages were measured 21 days after infesting and used to screen the genotypes. Despite the relatively high linear correlation between two resistance components, leaf rolling and leaf chlorosis, estimated as 80%, analysis of variance indicated significant differences among genotypes. Eight durum lines DW9, DW14, DW4, DW2, DW7, DW8, DW12 and DW3 and one bread wheat cultivar (Azadi) were significantly more resistant than the resistant control (Halt). One durum line (DW13) and two bread wheat cultivars (Shiroodi and M-75-7) had higher leaf rolling and leaf chlorosis than the susceptible control (Sholeh). So they were selected as the most susceptible to the biotype of D. noxia. under study. Orthogonal comparison showed that durum lines were more resistant than bread wheat cultivars. These results were confirmed by Cluster Analysis based on leaf rolling and leaf chlorosis.
I. Zarrinabadi, P. Ehsanzadeh,
Volume 7, Issue 4 (1-2004)
Abstract
Despite its economic importance, not much information exists on growth and productivity of durum wheat in Iran and Isfahan. In order to evaluate growth, grain yield, and attributes in three durum wheat genotypes, Osta/Gata, Dipper-6, and PI40100, with five planting densities of 200, 275, 350, 425 and 500 seeds/m2, a four-replicate RCBD field study with a spilt-plot layout was carried out in winter 2000 to spring 2001 at Lavark Research Farm, College of Agriculture, Isfahan University of Technology. Genotypes were the main-plots and planting densities, the sub-plots. Each plot contained six rows, spaced 25cm apart, each 6m in length. While Osta/Gata produced a greater spikes/m2, 1000-grain weight and grain yield, PI40100 indicated a greater height, leaf area index (LAI) and biological yield, and Dipper-6 produced a larger number of grains per spike and harvest index, compared to the other two genotypes. Increasing plant density led to an increase in LAI, spikes/m2, grain yield, biological yield and harvest index but a decrease in grains/spike and 1000-grain weight. Grain yield was positively correlated with spikes/m2, grains/spike and 1000-grain weight, despite negative correlations with LAI and plant height. It may be concluded that with environmental conditions such as those encountered in Isfahan in 2000-2001, Osta/Gata can be planted at approximately 425 seeds/m2, if an acceptable grain yield is to be achieved.
M. Eslami, S.a.m. Mirmohammady Maibody, A. Arzani,
Volume 9, Issue 3 (10-2005)
Abstract
To evaluate heritability, phenotypic and genotypic correlation coefficients, phenotypic and genotypic variances as well as relationships between some of the grain quality traits, an investigation was conducted in a randomized complete block design replicated three times in 2001, using four durum wheat genotypes PI40100, Dipper-6, Oste/Gata, Shova and their F2 and F3 progenies derived from their crosses. Grain quality characteristics including seed hardiness, protein content, SDS sedimentation volume and gluten content were evaluated. The results indicated that wet gluten content and dry gluten content had the highest coefficient of variation. Statistical analysis showed a significant difference between genotypes for all traits with the exception of SDS sedimentation volume. Dry gluten content had the highest heritability (90.4%) while SDS sedimentation volume had the lowest heritability (48.6%). Results of correlation analysis indicated that seed hardiness correlated significantly with protein content, SDS sedimentation and dry gluten content. Protein content had a positive correlation with wet and dry gluten content. Since dry gluten content showed a high correlation with protein content (quantity) and seed hardiness had a high correlation with SDS sedimentation volume, hence these two traits can be used as the selection criteria for improving quality of protein in durum wheat.
E. Farahani, A. Arzani,
Volume 10, Issue 4 (1-2007)
Abstract
An experiment was conducted to investigate the genetic diversity in the cultivars and F1 hybrids of durum wheat, using agronomic and morphological traits. Evaluation of consistency between choosing parents of crosses based on morphological and agronomic observations and genetic distance obtained from cluster analysis were objectives of this study. Fourty- two genotypes were evaluated using a randomized complete block design with three replications in 2003. Agronomic characteristics comprised days to 50% flowering, days to 50% pollination, days to maturity, plant height (cm), spike length (cm), grain weight per spike (gr), number of grain per spike, number of spike per m2, grain yield (t/ha), biological yield (t/ha), 1000 grain weight, test weight (gr/L) and harvest index were measured in the studied genotypes. The results of analysis of variance showed significant differences among genotypes for all the studied traits. The results also revealed that grain yield had the highest coefficient of variation (CV= 21.6%), followed by spikes per m2 (CV= 20%) and number of grains per spike (CV= 18.9%). Lowest coefficient of variation was observed in days to maturity, days to 50% pollination and days to 50% flowering. Moreover, correlation analysis indicated that grain yield correlated with harvest index, biological yield, grain per spike and grain weight per spike. Cluster analysis of genotypes based on agronomic traits defined desirable clusters. These clusters have desirable characteristics and are beneficial for breeding objectives. Results of evaluation of consistency between choosing parents based on observation and those of cluster analysis showed varing degrees of consistency in 12 conducted crosses, ranging from highly similar parents in Eupoda6×Chahba88 to relatively distinct parents in PI40100×PI40099 cross.
E. Farahani , A. Arzani,
Volume 11, Issue 42 (1-2008)
Abstract
An experiment was conducted to investigate the heterosis in 12 F1 hybrids of durum wheat using agronomic and morphological traits. Parents were selected according to the estimated genetic distances based on the results of a two-year field experiment, which were then crossed to produce F1 hybrids. Twenty-three genotypes (including 11 parents and 12 hybrids) were evaluated using a randomized complete block design with three replications Research Farm of College of Agriculture, Isfahan University of Technology, located at Lavark, Najaf- Abad in 2003. Agronomic characteristics comprised days to 50% flowering, days to 50% pollination, days to maturity, plant height, spike length, grain weight per spike, number of grain per spike, number of spike per m2, 1000-grain weight, biological yield, grain yield and harvest index. The results of analysis of variance showed significant differences among parents F1 hybrids and parents vs. crosses for all the studied traits. Substantial differences in the level of heterosis for plant maturity were detected among the hybrids with the highest heterosis belonging to HPI40100×PI40099 and HEupoda6× Chahba88 hybrids. Furthermore, superior hybrids included HAltar84/Ald×Chahba88, HBuchen7×Chahba88 and HEupoda6×Mexi75/Vic possessing the highest heterosis for grain yield and grain yield components among 12 hybrids of the present experiment. Eventually, it is concluded that Eupoda6, Odin12, Altar84/Ald and 45063Karaj genotypes when crossed with Mexi75/Vic genotype as well as Buchen7 and Altar84/Ald genotypes when crossed with Chahba88 genotype produced superior F1 hybrids.
S.r. Monfared, A. Hosseinzadeh, M. Mardi, M.r. Naghavi, S.m. Pirseyedi,
Volume 12, Issue 45 (10-2008)
Abstract
The genetic diversity of major crops, including durum wheat, has suffered an overall reduction with time. The knowledge of patterns of genetic diversity enhances the efficiency of germplasm conservation and improvement. In this study, 87 Iranian landraces of Triticum turgidum var. durum originating from different geographical areas of Iran, along with 21 durum cultivars from ten countries were evaluated using ten primer combination SSAP markers. Retrotransposons are mobile genetic elements that transpose via RNA mediation. They have wide distributions in genome because molecular markers have been designed based on them in recent years. SSAP markers BARE-1,Thv19, Tagermina and Tar1 were also used. Thv19M+ACA primer combination had the most polymorphic band in both landraces and cultivar durum wheats. Approximately 26.7 % BARE-1 bands were polymorphic in landraces.Thv19 showed a polymorphism level of 51.5%, and Tar1 and Tagermina displayed polymorphism levels of 32.8% and 27.2%, respectively. The amount of polymorphism in the studied cultivars for retrotransposons BARE1, Tagermina, Thv19 and Tar1 were 24.4%, 24.3%, 51.5%, 28.5%, respectively. This results show that Thv19 and Tar1 have more transpositional activity in the evolutionary process. Finally, Dendrogram was constructed to use algorithm UPGMA and Dice similarity coefficients.
H. R. Balouchi, S. A. M. Modarres Sanavy, Y. Emam, M. Barzegar,
Volume 12, Issue 45 (10-2008)
Abstract
Water deficit, ultraviolet radiation and CO2 concentration enhancement are three environmental stresses that affect nutrition of human in future. This research was conducted in the Research Greenhouse of Faculty of Agriculture, Tarbiat Modares University in 2006, in order to study leaf qualitative traits of durum wheat under different levels of carbon dioxide (400 and 900 ppm), ultraviolet radiation (UV A, B and C) and water deficit (up to %60 of field capacity). Ultraviolet radiation enhancement increased anthocyanin, flavenoids and carotenoids of durum wheat leaf. There was no significant interaction effect between carbon dioxide and water deficit on anthocyanin and carbohydrates. Also, the interaction effects among the three factors were not significant on anthocyanin, carotenoids, chlorophyll a and a+b. Water deficit decreased leaf proteins. Increasing intensity of ultraviolet radiation and carbon dioxide enrichment decreased soluble leaf protein in durum wheat under water deficit conditions. The results showed that three main environmental stresses which reduce leaf pigment and plant protection due to negative effects of these stresses along with soluble leaf protein reduction decrease qualitative and quantitative yield of the wheat variety.
M Gorji, H Eshghizadeh, A Khosh Goftarmanesh, A Ashrafi, A Moalem, N Poursakhi, N Pourghasemian, A Miladi,
Volume 12, Issue 46 (1-2009)
Abstract
Iron deficiency is a worldwide nutritional constraint in agricultural lands especially in calcareous soils. Cultivation of crops tolerant to Fe-deficiency is an approach to combat Fe deficiency. The aim of this investigation was to evaluate Fe-efficiency of selected important crops in Iran. A completely randomized block design in triplicates was conducted at IUT research greenhouse in fall 2006. Sweet corn (Hybrid K.S.C. 404), grain corn (Hybrid S.C. 500), safflower (cvs. S3110, S-411), sunflower (Hybrid Hyson) and durum wheat (cv. Shuga) were grown in a nutrient solution at two Fe levels (1 and 10 µM Fe-EDTA). The results showed significant (P < 0.01) variation among the studied crops in Fe-efficiency. Corn hybrids were more sensitive to Fe deficiency (FeE = 26%) as compared to other studied crops, and the greatest reduction was observed in their shoot dry matter at 0.1 mM Fe- EDTA treatment. In contrast, the lowest decrease in root and shoot dry matter weight under Fe-deficient condition was found for durum wheat (FeE=94%). Comparing the calculated Fe-efficiency using different indices showed that Fe concentration and content in the whole plant, shoot and root had no relationship with crop tolerance to Fe deficiency.
