Showing 3 results for Electrophoresis
Farhad Ghavami, Abdolmajid Rezai, Siroos Abdemishani, Ahmad Arzani,
Volume 3, Issue 2 (7-1999)
Abstract
Variability of seed storage protein electrophoretic patterns revealed by SDS-Polyacrylamide gel electrophoresis and their possible relations with some morphological and phenological characteristics were studied using 193 accessions from Iran Mungbean (Vigna radiata L. Wilczek) Collection. Seed protein electrophoresis revealed six different patterns that were different in two albumin and four globulin subunits with molecular weights of 23500-34000. Pattern types 1 and 2 had a considerable frequency but pattern types 3, 4, 5 and 6 had low frequencies and probably had developed recently in mungbean evolution process. Cluster analysis of countries and cities using the frequencies of albumin and globulin subunits showed no relation between geographical diversity and similarity distances. The correlations of morphological traits and albumin and globulin subunits indicated a relationship between G1 and G2 subunits with 1000 seed weight and days to beginning of maturity. Therefore, selection based on protein patterns at preliminary stages of breeding programs might be effective in increasing seed yield together with earliness.
G.h. Tahmasebi, D. Qujeq, R. Ebadi, M. Akhoundi,
Volume 3, Issue 4 (1-2000)
Abstract
An important and fundamental step in breeding honeybees is the separation of honeybee populations in Iran. During the years 1992-1996, several research works were directed towards the investigation of genetic populations of the Iranian honeybee (Apis mellifera L.), using biochemical methods. For this purpose, samples of worker bees were taken from 251 colonies of 115 apiaries in 84 cities in 21 provinces. The biochemical studies were conducted on thoracic proteins of worker bees using the horizontal electrophoresis.
The thoracic proteins of worker bees from the north and east populations isolated by electrophoretic techniques lacked a protein band having molecular weight of about 100,000 daltons. The results obtained from morphological and biochemical techniques and segregation of populations, indicate that breeding centers can be established in northern as well as western and central regions of Iran to improve genetic potentials of Iranian honeybee population.
M. R. Dini Torkamani, J. Karapetion,
Volume 11, Issue 40 (7-2007)
Abstract
Sesame is an annual self-pollinated plant with a high seed oil and protein content. It is considered as an oilseed plant for human consumption. For a breeding purpore, quantitative and qualitative characteristics of seed total protein of different sesame varieties (Oltan, Zoodras , Yekta , Hendi , Varamin , Chini , Karaj 1 , Moghan local,Nazok tak sakheh and Nazok chand shakheh) were evaluated. Protein extracts were analysed using Kejeldal and gel electrophoresis methods. Total average of 24.02% was observed for seed protein content of all varieties. Analysis of varieties revealed a significant variation at (p < 0.01) between varieties. The profile of seed storage proteins had a relative mobility from 0.20 to 0.94. The observed bands varied in intensity and scattered in three zones of the gel . All bands were monomorphic and no variation was observed between the varieties. It was concluded that this characteristic does not carry the necessary value for breeding purposes.
