Showing 5 results for Endophytic Fungi
R. Mohammadi, A. F. Mirlohi,
Volume 7, Issue 2 (7-2003)
Abstract
A symbiotic relationship exists between the endophytic fungi of the genus Neotyphodium and many cool-season grasses. Endophytes can alter the growth as well as morphological and physiological characteristics of the host plant, thereby influencing the persistence and survival rate of infected plants. This study was conducted to evaluate the effects of endophyte on phenotypic characteristics of Iranian tall fescue (Festuca arundinacea Schreb.) and meadow fescue (Festuca pratensis Huds.). The experiment was set up as a completely randomized design with three replications in a factorial arrangement. The first factor was the two plant accessions and the secondary factor was the endophyte-infected and noninfected plants. The results showed that most of phenotypic characteristics significantly altered as a result of endophyte infection in both tall fescue and meadow fescue plants. Results of analysis of variance showed that the influence of endophyte fungus was positive and significant on tiller number, herbage yield, dry crown weight, dry root weight per plant, and crown depth. Also between plant accessions, there were significant differences for dry herbage yield, crown weight, dry root weight per plant, crown depth, and dry matter percentage. Plant by endophyte interaction was highly significant for crown depth and dry root weight per plant. Therefore, eudophytic fungi can be used to improve phenotypic characteristics in these plants.
B. Hatami, A.f. Mirlohi, M.r. Sabzalian,
Volume 10, Issue 2 (7-2006)
Abstract
Endophytic fungi, Neotyphodium spp. symbiotically infect host grasses, Fescue arundinacea Schreb. and F. pratensis Huds. and Mealybug, Phenococcus solani Ferris attacks some forage plants. To evaluate the role of these fungi to control this pest, four genotypes of F. arundinacea and two genotypes of F. pratensis were used. Plant tillers were split into two sections, one section of which was freed from endophyte using a fungicide mixture. The mixture contained 2 grams of active ingredients of Propiconazol and 1 ml of Folicur per liter of water. New tillers from endophyte-infected and endophyte-free plants were transferred into the field. The number of mealybugs was measured after the first visible sign of infestation on roots. To count mealybug, one plant hill of each plot was randomly selected. Hay yield of each plot was measured by clipping the plants 5 cm above ground level. Correlation of hay yield and other growth characteristics with the number of mealybugs on roots as an index for mealybug damage on infested plants was determined. The results indicated that endophyte-free plants were highly infested with mealybug, P. solani compared with endophyte-infected plants that were completely free of P. solani, correlation coefficients showed that there was significant negative correlation between forage yield and mealybug numbers, indicating mealybug damage on infested plants. It seems that endophytic fungi are effective biocontrol factors for some root feeding pest such as mealybug, P. solani.
M. Parsaeian, A. F. Mirlohi, A. M. Rezaie, M. Khayyam Nekoie,
Volume 10, Issue 4 (1-2007)
Abstract
To determine the role of endophytes in conferring valuable physiological characteristics on and induction inducing cold tolerance in two species of festuca, an experiment was done at Isfahan University of Technology in 2002. Endophyte-infected and non-infected clones from two genotypes of tall fescue and one meadow fescue were prepared and coded 75,83 and 60 respectively. The clones were exposed to cold treatments at 6, -2 and –10C and compared with control treatment at 20C. After three weeks of cold treatments, relative water content of leaf and crown, cell membrane stability (electrolyte leakage), percentage of membrane damage and finally proline content of leaf were measured. The presence of endophyt caused an slight increase in relative water content of leaf and crown. There was considerably higher proline in endophyte-infected plants compared with non-infected ones in both stress and non-stress conditions. Endophytic fungi had strong effects on maintenance of membrane stability and on the decrease of electrolyte leakage at all temperature levels. Among plant genotypes, 83 preformed better for some characteristics, specially in the presence of endophyte, and showed higher cold tolerance.
M. M. Majidi, A. F. Mirlohi,
Volume 11, Issue 1 (4-2007)
Abstract
Seed traits are an important measure of the effects of endophytic fungi in Festuca genus. In this study, endophyte-infected (E+) and un-infected (E-) clones of the same tall fescue genotypes were used to investigate the effects of endophyte on the seed production and associated traits. Endophyte infection resulted in 32.8% increase of total seeds by weight, 34.6% seeds per plant, and 30.6% panicles per plant, but other seed components were not affected by endophyte. Significant phenotypic variances were observed for traits except for 1000 seed weight. Plant genotype×fungal status interactions occurred for seed per plant. These interactions occurred largely because of variation in different plant genotypes. Genetic variance for this trait in E+ was greater than E- for both years, suggesting that the endophyte can mask plant genotypic variance. The results indicated that endophyte had a positive effect on seed production but could result in overestimating of plant genetic variance for some traits. In conclusion, breeding strategies should consider presence or absence of endophyte and the possible effects on variances.
M. M. Majidi, A.f. Mirlohi, M.r. Sabzalian,
Volume 11, Issue 41 (10-2007)
Abstract
Fescues (Festuca spp) are widely distributed in the temperate regions and are used for turf, forage and soil conservation. Though seed traits are important measures of the effects of endophytic fungi on their host, little information is available in this respect for Festuca. In this study, endophyte-infected (E+) and endophyte-free (E-) clones of the six fescue genotypes were used to investigate the effects of endophyte on seed production. The relationships between seed traits plus direct and indirect effects of components on seed yield were also studied. Endophyte infection resulted in 38.1% to 249% more seed yields in some genotypes, though some were not affected by this symbiotic relationship. Correlations between traits and also results of stepwise regression were influenced by the presence of the symbiotic fungi. Path analysis showed that endophytic fungi changed direct and indirect effects of components on seed yield. In endophyte containing clones, panicle fertility had the most direct effects and number of panicle per plant and seed per panicle the most indirect effects on seed yield. In E- clones, panicle fertility had the highest indirect effects and other components had direct effects on seed yield. The results suggested that breeding strategies for increasing seed yield in fescue should consider the presence or absence of endophyte in germplasm.
