JWSS - Journal of Water and Soil Science

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Potassium fixation and release by phillosilicate clay minerals in soils are very important processes influencing the availability of K to plants. This investigation was conducted to determine the potassium fixation capacity and charge characteristics of soil clays of 15 surface soils (0-30 cm) from central and northern Iran. After clay particle separation, both total and tetrahedral cation exchange capacity of soil clays were determined. Tetrahedral CEC was measured after saturation with Li and heating at 300 C to reduce octahedral charge to near zero. Potassium fixation was obtained in both wet (1:10 soil:solution, 16 h on a shaker) and dry conditions (after drying for 24 h at 70°C) using three different levels of added K

The total CEC in soil clays of Isfahan, Char-Mahal and Gilan provinces ranged from 22.1-36.0, 33.0-55.8 and 31.3-47.9 cmol kg^-1, respectively. Tetrahedral CEC in soil clays of Isfahan, Char-Mahal and Gilan provinces ranged from 17.9-4504, 26.2-32.5 and 8.3-23.8 cmol kg^-1, respectively, which consisted of 81.0-98.4, 58.5-95.8 and 24.7-72.5% of their total charge, respectively. The amount of K fixation increased with drying and the level of k added. Mean potassium fixation in soil clays of Isfahan, Char-Mahal and Gilan ranged from 5.42-9.13, 6.63-14.67 and 8.87-10.36, respectively. Mean potassium fixation by soil clays (except for soil clays of Gilan) best correlated with total CEC. In the soil clays of Isfahan, mean potassium fixation correlated with tetrahedral CEC, whereas no correlation was observed in soil clays from other places. The average amount of potassium fixation in clay fractions was in the order: Gilan clays > Char-Mahal clays > Isfahan clays.

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Environmental pollution is one of the major problems for humans and animals. Radioactive wastes and heavy metals are important sources of pollution. Recent studies suggest that soil and geomedia have a high potential for the disposal of radioactive wastes and heavy metals. The clay minerals in geomedia can act as a barrier against radioactive wastes and heavy metals. However, enough information and experimental evidence about the mechanisms involved in the fixation of metals by clays are not available. This study aimed to show the effect of clay type on the fixation of cobalt and cadmium as affected by thermal energy. Increasing the temperature from 25 to 400°C decreased the CEC of clays in Ahwaz and Darab soils saturated with cobalt from 35.6 and 50.1 cmol_c+/kg to 28.8 and 4.5, respectively. In cadmium clays, however, reductions in CEC were minimal (from 34.3 and 51.2 at 25°C to 32.5 and 47.7 cmol_c+/kg at 400°C, respectively) compared to cobalt saturated clays. Consequently, the bonding characteristics change according to the size of the cations upon thermal treatment. The small cation, cobalt with an ionic radius of 0.68 Å, apparently migrates to the octahedral vacant sites and forms covalent bonds after heating at 400°C to become non-exchangable (fixed). The larger cation, Cd with an ionic radius of 0.97Å, apparently does not migrate into lattice sites on thermal treatment and remains exchangeable.

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Azospirillum brasilense is one of the N2-fixing microorganisms which, in symbiosis with the roots of cereals and other graminae, enhances growth and development of plants. In this research, wheat seeds (Triticum aestivum) of three cultivars (Ghods, Roshan and Omid) were inoculated with Azospirillum strains (Sp7 and Dol). Inoculation increased growth parameters and yield of wheat cultivars, the effect being directly dependent on the strain-cultivar combination. Strain Sp7 induced the greatest grain yield, 1000-seed weight, number of grains per spike, root and shoot dry weight in Cv. Roshan, whereas strain Dol stimulated the best effect on these growth parameters in Cv. Ghods. The response of Cv. Omid was lower as compared to the other cultivars. Thus, it may be concluded that compatible strains are necessary for increasing yield and enhancing growth and development of wheat cultivars. These experiments also illustrated similar results regarding the effect of strains on N content of seeds. The observation of nitrogenase activity of Azospirillum strains in the In vitro experiments and the significant increases in N content in some inoculated cultivars support the hypothesis that biological nitrogen fixation by Azospirillum could be responsible for the observed beneficial effects on growth parameters. The comparison of nitrogenase activity of two strains showed that acetylene reduction rate for strain Dol was 1.5 times more than that for strain Sp7. Also strain Dol had more pronounced effects on growth parameters, yield and N content of grains than did strain Sp7. Strain Dol is a local strain whereas strain Sp7 is a Brazilian isolate thus, it may be concluded that the local isolates should be preferred to the foreign bacteria, as they are better suited to traditional cultivars, environment and soil conditions of the region.

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Heavy metals have deleterious the effects on nodulation and N₂ fixation of Rhizobium- Legume symbiosis, due to their inhibitory effects on the growth and activity of both symbionts. This research has been undertaken to evaluate the effect of Cd tolerance of native rhizobial strains on diminution of the Cd detrimental effects on Sinorhizobium meliloti-alfalfa symbiosis. For this purpose, a greenhouse experiment was conducted based on Randomized Complete Block Design. The treatments in this experiment included: plants inoculation with 6 bacterial strains (sensitive, partially tolerant and tolerant to Cd), 5 levels of Cadmium (0, 2, 5, 10, 20 mg/kg soil) and non-inoculated control. In different levels of Cd, the effects of bacterial inoculation on root nodule number and total amount of nitrogen in plant shoot were compared. The results indicated that soil pollution by Cd even at 2 mg/kg had significant effect on symbiotic properties of rhizobial strains, and according to Cd tolerance of various strains, the mentioned effect was different. Decreasing effect of Cd concentration on root nodules and nitrogen concentration in plants that were inoculated with sensitive strains in comparison with plants inoculated with tolerant strains was 68.31% and 40.8%, respectively. In this research, R_95m was introduced as the best strain because of its ability for nodulation and nitrogen fixation.

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Sugarcane (Saccharum officinarum L.)‌ and rotational crops have been cultivated without potassium fertilizers for many years in southwestern Iran. Although potassium was removed from this soil, no response has been reported to K fertilizers by crops. This study was conducted to evaluate the effects of wetting and drying, and cultivation systems on potassium fixation in some Khouzestan soils. The results showed that expandable clay mineral was observed in cultivated soils but not in the non-cultivated soils. This may be related to irrigation, and cultivation practices in this soils. By adding K and sequential wetting and drying the amount of K-available increased in sugarcane, rotational cropping and uncultivated soils from 132.6, 226.2 and, 171.6 mgkg^-1 to 266, 447 and 628, respectively. These results showed that more K can be fixed after cultivating soils, especially by sugarcane, but available K increased by adding K. All surface soils had higher K fixation capacity than subsurface ones. Also, by application K to these soils, the amount of K fixation decreased with increasing wetting and drying times. The K fixation increased by the increase of cation exchangeable capacity. Significant difference was observed between cultivation system and depth of sampling in 1% levels. High K fixation can be attributed to illite minerals depleted from K. Mica-like minerals formed after adding K, and wetting and drying cycles. Amount of K fixation by clay particle samples was more than the same soil samples but in both the same trend was observed for K fixation. Also, drying and wetting decreased K fixation in the last periods. It may be due to trapping K in the interlayer positions.

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Azotobacter chroococcum is an important PGPR (Plant Growth Promoting Rhizobacteria) producing compounds needed for plant growth. The aim of this research was to study the effects of different native strains of Azotobacter chroococcum on growth and yield of wheat under greenhouse counditions. Seeds of spring wheat (Triticum aestivum L. var. Pishtaz) were inoculated with some Azotobacter chroococcum strains capable of producing IAA, HCN, sidrophore and fixing molecular nitrogen. The inoculation of wheat with those strains had a positive, significant effect on biological yield, seed protein percentage, thousand seed weight, leaf area, N, P, Fe and Zn uptake, in particular, by wheat. The increased growth of wheat was most likely due to the production of IAA and enhanced nitrogen fixation by inoculated strains. Some strains of Azotobacter chroococcum native to Chaharmahal va Bakhtiari are established as PGPR. Results also support the efficiency of Azotobacter chroococcum as an important biofertilizer in wheat cropping systems. The selected strains had a significant effect on wheat growth and yield, including biological yield and seed quality under greenhouse counditions. This beneficial effect of Azotobacter chroococcum on wheat is attributed mainly to IAA production and, to some extent to non symbiotic nitrogen fixation in the rhizosphere. So, these strains can potentially be used to improve wheat nutrition of micronutrients such as Fe and Zn, in particular.

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In order to study the effect of air and root-zone temperature on yield, yield components, nodulation and nitrogen fixation of three annual medics, an experiment was conducted in controlled environment (growth chamber) at the Faculty of Agriculture, Tarbiat Modares University in 2006. The experiment was performed as a spilt split plot with the layout of completely randomized design with three replications. Air temperature at three levels including 15/10, 20/15 and 25/20ºC day/night, four levels of root-zone temperatures including 5, 10, 15 and 20ºC and three annual medics (Medicago polymorpha, M. radiata and M. rigidula) were randomized to main plot, sub plot and sub sub plot units, respectively. The results showed that there were significant differences among annual medics for dry matter production, yield components and nitrogen fixation. M. rigidula produced more leaves, stems and root dry matter, leaf and stem to root ratio, leaf number and area and forage yield than other annual medics. Also, three annual medics at 25/20ºC day/night air temperature (the highest one) produced more nodulation dry matter (8.85 mg/pot) and nitrogen fixation (7.7 mg/g dry matter) than other temperatures. Plants at the former temperature produced 8 and 2 times more nodulation and nitrogen fixation than 15/10ºC day/night air temperature (the lowest one), respectively. Low root-zone temperature up to 5ºC had severely negative effect on yield and nitrogen fixation in the three studied annual medics. Interaction among annual medics, air and root-zone temperatures showed that M. rigidula was better than other annual medics for yield, nodulation and nitrogen fixation at 25ºC air temperature and 15ºC root-zone temperature . The result showed that M. rigidula had normal growth and development compared with other annual medics at low root-zone temperatures. Thus, M. rigidula may be a better annual medic for cultivation in cold and moderate regions. Therefore, in the zones where soil temperature is lower than 5ºC during the season, cultivation of annual medics is not successful, but in the zones where soil temperature is greater than 10ºC, annual medics have normal growth and produce average yield due to better nitrogen fixation.

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Potassium (K) fixation by phyllosilicates clay minerals is a very important process influencing the availability of K for plants. Information about K fixation is limited in Hamadan soils. The objectives of this research were to determine potassium fixation capacity (PFC) and potassium fixation index (PFI) of 10 surface soils of Hamadan province and the correlation of these parameters with soil charge characteristics. Potassium fixation capacity was obtained using six different levels of added K as KCl and three wetting and drying cycles. Also charge characteristics of soils were determined. Tetrahedral CEC of soils was determined after saturation of samples with LiCl and heating at 300 oC to reduce octahedral charge near zero. The results showed that tetrahedral and octahedral CEC ranged from 4.6 to 16.0 and 0.8 to 4.9 cmolckg-1 respectively. Mineral and organic portion CEC ranged from 6.1 to 20.9 and 0.97 to 9.7 cmolckg-1 respectively. The amount of PFC increased with increasing added K. Mean amount of PFC ranged from 58.2 to 175.03 mgkg-1. Potassium fixation index ranged from 0.23 to 0.67. The results of correlation studies indicated that PFI were significantly correlated with octahedral CEC, mineral and organic portion CEC, total CEC, exchangeable and nonexchangeable K. The results of this research showed that PFI very different in soils. So that this parameter should be consider in K soil testing.

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Azotobacter chroococcum can improve mineral nutrition of plants through N2 fixation and plant growth promoting capabilities. Fourteen strains of A. chroococcum were isolated from rhizosphere of wheat plants grown in different field conditions around Tabriz, northwest of Iran. In a pot culture experiment with sterile soil, wheat plants (Triticum aestivum cv. Falat) were inoculated with 14 bacterial strains. Positive control received nitrogen fertilizer without bacterial inoculation and the negative control was left un-inoculated and without N- fertilizer. Totally, 16 trearments with four replications were arranged in a completely randomized design. The plant growth indices and N and P concentrations of shoot and root were determined at the harvest time. Results showed that the inoculation with Azotobacter strains caused a significant increase in shoot and root dry weights. Bacterial inoculation significantly enhanced the concentration and content of N in shoot and root. Phosphorus content was only enhanced (p<0.05) in the root. Translocations of N and P from root to shoot were markedly increased in bacterial treatments compared to the positive and negative controls. Moreover, strains 1 and 48 which showed relatively higher phosphate solubilizing capability and phosphatase activity in in-vitro assay also brought about higher P content and concentration in shoot and its translocation from root to shoot.

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Although addition of amendment materials to soil is not for changing of soil K status, secondary effects of these materials can be important in K distribution among soluble, exchangeable and non-exchangeable forms. In this research, effect of addition of 2% zeolite, 2% vermicompost and 1% zeolite+ 1% vermicompost on 10 calcareous soils of Fars province belonging to five orders was investigated. The experiments were done as a randomized complete block with three replicates. Samples were incubated at 22±2 °C and 50% of saturated moisture content for 90 days. Then different forms of K were determined in the samples. Zeolite application induced 279 mg kg-1 increase in exchangeable K and 24 mg kg-1 decrease in non-exchangeable K, but it had not a significant effect on soluble K. Although vermicompost application increased soluble and exchangeable K, its effect was more sensible for soluble K (139 mg kg-1). Zeolite+vermicompost application increased all forms of K. Increase in soluble K with vermicompost application may increase risk of K leaching but zeolite application can preserve K in exchangeable phase and inhibit K leaching and fixation.

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This study was done to compare K+ and NH4+ fixation in different agricultural systems in Khuzestan province. Soil samples were collected from Shushtar and Abadan. Samples were collected from depths of 0-30 and 30-60 cm in sugarcane, multi cropping, palm and uncultivated land. Then, 0-50-100-200-400-600-800-1000 mg kg-1 K+ or NH4+ concentrations were added to 0.5 gram of clay separated from these soil samples. These clay samples were kept for 24 h at room temperature. Then, they were extracted with 0.5 M magnesium acetate, and K+ and NH4+ were determined by flame photometer and micro diffusion methods, respectively. Fixed K+ or NH4+ was determined from added and extracted cations. Also, XRD studies were done in some samples. Kaolinite, palygoreskite, illite, chlorite, smectite and quartz were identified by XJRD. Statistical analysis showed that K+ or NH4+ fixation and rate were increased by increasing the concentration. Maximum K+ or NH4+ were fixed in surface layer’s clay in Shushtar multi cropping and Abadan Palm cultivation. The results showed that K+ and NH4+ fixation were increased by increasing the concentration. But, under increasing concentration for a cation, fixation decreased for others and vice versa. NH4+ fixation was more than K+. This may be due to smectite clay mineral in these samples. Smectite group tend to cause NH4+ fixation more than K+. These results can be applied for K+ or NH4+ fertilizers management.

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Current study conducted to evaluate the effects of vermicompost and zeolite on the kinetics of nickel (Ni) fixation. Treatments consisted of a factorial combination of two vermicompost levels (zero and 2 w/w percent), three zeolite levels (zero and 4 w/w percent of zeolite of Firoozkoh, and Semnan) and two soil textures (clay and sandy loam) in three replications. All treatments were spiked with 50 or 100 mg Ni kg-1. DTPA extractable Ni was determined after 5, 10, 20, 30, 60 and 90 days. Ni availability was higher in sandy loam texture. Vermicompost application increased Ni availability in sandy loam texture in all the designated times. Zeolite application had no significant effect on Ni availability. The trend of Ni availability decrease was composed of two distinct stages with high and low Ni fixation rates. In the first step which continued up to 30 d, the available Ni fixation rate was high and then decreased sharply. Ni fixation data was suitably prescribed using simple Elovich and exponential equations. It seems that vermicompost has a greater effect to prevents Ni fixation and to retain it in available form in light texture soils. On the other hand, it seems that zeolite does not have any considerable effect on Ni fixation in calcareous soils.

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Potassium (K) fixation in soil is an important process which affects the availability of K to plants. There is limited information on K fixation capacity (PFC) of soils in Chahar Mahal & Bakhtiary province. This study was carried out with the aim of determining PFC and its relation with soil characteristics in 10 samples of surface soils in Chahar Mahal & Bakhtiary province. PFC was determined by adding six different levels of K by using KCl salt and measuring cation exchange capacity (CEC) in mineral, organic and clay fraction of soils. The results showed that the CEC in the mineral, organic and clay soils changes from 16.02-19.38, 1.71-3.27 to 29.3-39.8 cmol+/kg consequently. Potassium availability index (PAI) in the soils changes from 0.36 to 0.7 and Potassium fixation index (PFI) changes from 0.29 to 0.63. According to the results PFI and PAI showed significant correlation with whole and mineral fraction of the soil CEC, clay percent, pH and CaCO₃ percent. The results of this study also showed that fixation of K fertilizer in the studied soils plays an important role in K dynamic of soil and K availability to plants.

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