JWSS - Journal of Water and Soil Science

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In order to study fungi associated with seeds of sainfoin, several seed samples of sainfoin were collected from Isfahan, Ardabil, Zanjan and eastern Azerbaijan provinces, Iran. Seed lots, only half of which were surface sterilized, were allowed to grow on PDA, SMA, MA, sterilized wet paper and drenched sands. The dishes containing seeds were incubated at 25°C with alternate cycles of 12-hour fluorescent light. After seven days, fungi growing from the seeds were transferred to the selective media and subjected to morphological studies. Single spore or hyphal tips from these transfers were the basis for all identification procedures. The most prevalent fungi associated with sainfoin seed were Alternaria, Aspergillus, Penicillium and Rhizopus, although Ulocladium, Cladosporium, Fusarium, Mucor, Nigrospora, Stemphylium, Trichothecium and Botrytis were also isolated in low incidence from these seed lots. The isolates of Uromyces and Oidiopsis were also obtained when the seed samples were examined by washing method.

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Seeds of Haloxylon (H. pericae, H. aphyllum, and H. ammmodendron) were collected from Sabzevar and Gonabad regions. The seeds were kept in the refrigerator for isolation of the fungi after determining the germination rate and moisture content. The recommended methods by International Seed Testing Association (I.S.T.A.) for isolation of seedborne fungi were followed throughout this study. The fungi were isolated by agar plate method, standard blotter technique, Hiltner test and seed analysis. In this study 15 genera and 19 species of fungi were identified. Among them Alternaria, Aspergillus, Camarosporium, Fusarium, and Penicillium were dominant among fungal genera isolates.

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The effects of growth of two strains of lactic acid bacteria on microbial flora of ground beef after packaging in air permeable packs were studied under workshop conditions. The strains isolated from dairy products were added to ground beef at a density of 10⁴ CFU/g after identification and antibiosis test against indicator bacteria (Escherichia coli and Staphylococcus aureus). The two strains were Lactobacillus casei subsp. casei 102, and Lactococcus lactis subsp. lactis var. diacetylactis 202. Inoculated and control samples were stored for up to five days under aerobic conditions in cold storage facilities with a temperature fluctuation between of –1°C to 5°C). Enumeration of different bacterial groups (total aerobic microorganisms, lactic acid bacteria, Pseudomonas spp., coliforms, Staphylococcus aureus, fungi) and detection of Escherichia coli were carried-out during storage period. The number of lactic acid bacteria in samples inoculated with lactobacillus casei subsp. casei 102 (treatment 1) increased during storage period, whereas total number of aerobic microorganisms, Pseudomonas spp., coliforms, and yeasts reduced. In samples inoculated with Lactococcus lactis subsp. lactis var. diacetylactis 202 (treatment 2), the number of yeasts decreased considerablely. As a result, Lactobacillus casei subsp. casei 102 can be used as a suitable antagonist of spoilage and pathogenic bacteria in ground beef under aerobic Conditions in cold storage facilities with a temperature variation between –1°C to 5°C.

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This experiment was conducted to evaluate the effects of adding acetic acid to drinking water on the performance and ileal microorganisms of broiler chickens. In a completely randomized design with 5 treatments and 5 replicates, 300 day-old commercial mail broiler chicks (ROSS) were divided into 25 groups, 12 chicks per group. Each of the five levels of acetic acid added to drinking water (0.0, 0.1, 0.2, 0.3, and 0.4%) were given to five replicates for a period of 21 days. All groups were fed a practical corn-soybean based diet. During the experiment, chickens were not fed any antibiotics or coccidiostats. The diet formulation for all groups was the same. At 14 and 28 days of age, one chicken of each replicate was weighed and killed by cervical dislocation. Ileal contents were collected and used for microbial evaluation. At 49 days of age, one bird from each pen was killed to weigh gastrointestinal tract, liver, pancreas and abdominal fat. The number of total aerobes and colliforms per gram of ileal contents were enumerated on the appropriate bacteriological media. There was no significant difference in feed intake, weight gain, feed to gain ratio, live body weight, gastrointestinal tract, abdominal fat, liver and pancreas weights (p>0.05). Also, the differences among treatments for total aerobe and coliform counts were not significant (p>0.05). Under the conditions of this study, addition of acetic acid as an organic acid into drinking water at the levels used could not affect the performance and ileal microbial counts of chickens.

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Tuberose is one of the tropical and subtropical bulbous cut flowers cultivated extensively in most floricultural regions of Iran. Although it has a high potential for a long vase life after harvest, tuberose declines rapidly at home. In order to overcome this problem, two experiments were conducted on a local cultivar, “Goldorosht-e-Mahalat”, using a completely randomized design. To find a suitable preservative which provides the longest vase life for tuberose, the experiment was carried out by applying the carelessness of most consumers: not recutting stem ends nor changing the vase solutions. In the first experiment the preservative solutions were: sucrose (1, 2 and 3%), silver thiosulphate (0.4, 0.8 and 1.2 mM), silver nitrate (50, 100 and 150 mgl^-1), citric acid (150, 300 and 450 mgl^-1) and tap water as the control. In the early days of the experiment, silver thiosulphate caused severe burning of the florets, silver nitrate caused the wilting of the florets and bent the end of the flower spikes and sucrose didn’t have any positive effect, but decreased the vase life. The longest vase life belonged to citric acid, after which the control (tap water) was placed. The second experiment was conducted to determine the role of the water quality and citric acid. The treatments were: sterilized distilled water, citric acid made with sterilized distilled water (150, 300 and 450 mgl^-1) and tap water as the control. The longest and the shortest vase life belonged to sterilized distilled water and the control (tap water) respectively. The citric acid prepared with sterilized distilled water had a desirable effect on the vase life of cut tuberose flowers. This effect increased with the increment of the acid up to 450 mgl^-1.

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The effects of Chickpea (Cicer arietinum L.) varieties, and sowing dates on weed interference were investigated in a 2-year (2002-3, and 2003-4 growing season) field experiment in Agricultural Research Station of Kohdasht in Lorestan Province. The experimental design was a randomized complete block in factorial arrangement with 3 replications. The experiment had 3 factors: weed interference at 2 levels (weed free, and weed infested throughout the total growing season), planting date at 3 levels (autumn, winter, and spring) and Chickpea varieties at 3 levels (ILC482, Hasham, and Greet). Weed density in autumn sowing plots was more than 3 and 7 times greater in autumn chickpea than in winter and spring sowing plots, respectively. Weed biomass in autumn sowing was 2.5 times as much as winter or spring sowing. Wild safflower and volunteer barley were the most frequent among the weed species. Volunteer barley was mainly present in autumn and winter planting dates. Wild safflower was among the weed species that in addition to competition, caused much difficulty to chickpea harvesting. Based on the hyperbolic curve fitted to the data, Chickpea maximum biomass reduction due to weed competition was estimated to be 91.8 %.

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Flora identification of each region plays an important role in the maintenance of natural resources of each country and it is a prerequisite to supplementary phylogenetics and genetic diversity studies. During February 1994 to August 1995, in a two-week interval, vineyards in two districts of Isfahan province, namely Zarinshahr and Tiran & Karvan were visited and weed samples were collected in flowering stages. All collected specimens were identified using specimens present in Herbarium of colleges of agriculture and natural resources of Isfahan University of Technology and other available references. The analysis revealed that in Tiran and Karvan, 84 species belonged to 71 genera and 26 families and in Zarinshahr 62 species belonged to 51 genera and 23 families. Species dominance in both districts belonged to Asteraceae and Gramineae families. Analysis of plant life forms using Raunkiaar method showed that Therophytes with 79.5% and Geophytes with 8.3% were the most frequent life forms in both Zarinshahr and Tiran & Karvan regions.