JWSS - Journal of Water and Soil Science

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In order to produce Tristeza virus-free plants from Satsuma mandarin (Citrus unshiu) cultivars (Ishikawa, Sugiyama and Myogawa), unfertilized ovules of plants suspected of CTV (Citrus Tristeza virus), from Mazandaran Mahdasht orchard were cultured on 4 media.

 Embryogenesis of nucellus tissue of ovules from Ishikawa and Sugiyama cultivars on MS medium were 31% and 25% and on MT medium from Ishikawa was 40.5%. Unfertilized ovules of Myogawa did not germinate on all media.

Nucellar embryos of Ishikawa and Sugiyama developed on MT+GA3+ME were 83% and 95%, respectively. Embryo differentiation, leaf and plantlet formation were observed on MT+GA3+ADE+ME medium. In the stage of two leaves, the plantlets’ growth under 1500 lux illumination was twice its growth under 800 lux illumination. Budding of in vitro plantlets from both cultivars on susceptible to Tristeza and virus-free seedlings of Key Lime did not show any infection by Tristeza.

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In this study, the control method of greater wax moth using male sterilization technique with gamma ray and chemical control methods were examined and compared. In order to determine the safe and effective dosage of gamma-ray to sterilize male pupae of the greater wax moth, an experiment was conducted in a complete randomized design with 5 treatments and 3 replicates with 50 male pupae in each replicate. Treatments included different levels of gamma-ray dosages with zero, 250, 300, 350 and 400 gray.

Release ratio of sterile to normal males was also studied in a similar experiment. Treatments included sterile-males, normal males and virgin females with the ratios of 1:1:1, 2:1:1, 3:1:1, 4:1:1 and 5:1:1. In this study, male pupae were irradiated with 350 grays of gamma-ray. The possible parthenogenetic reproduction of this pest was also studied. In order to compare the results from the male-sterile technique and the chemical control of this pest, another experiment was conducted in wax stores using 3 treatments with 3 replicates. Phostoxin was used as the most common chemical pesticide with a dosage of 1 tablet of 3 grams of 56% per 1 m3 space for the control of this pest.

The results showed that the best dosage to sterilize male pupae of the greater wax moth was 350 grays of gamma-ray. Also the best release ratio was four sterile male, one normal male for each normal female (4:1:1). Our results indicated that the females of the greater wax moth were not able to reproduce through parthenogenesis. There was no significant difference between the chemical and male-sterile methods for the control of this pest.

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The effect of different media including the growth regulators on somatic embryogenesis from Mexican lime was studied. The average embryo formation after 60 days from unfertilized ovules in different media was evaluated at 0 to 33.75%. The most effective hormone to stimulate embryogenesis was GA₃ at 0.01 and 0.1 mg/l concentrations. Malt extract (ME) at 300 mg/l increased embryo formation, whereas at 1000 mg/l, it inhibited embryogenesis but produced embryogenic callus. Banzyl adenine (BA) at 0.1 and 0.01 mg/l concentrations had significant effects on embryogenesis, while embryo formation was inhibited at high concentrations. Embryo development in different media was also studied. The index of mean root length to mean stem length in the MT³ medium supplemented with 0.1 mg/l of GA₃ was observed to be 1.22±0.22, which was regarded a suitable medium for embryo formation, embryo development and plantlet regeneration.

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To obtain a suitable explant and efficient culture medium for plant regeneration in spinach, two cultivars of Melody and Karaj local seedlings were chosen. A hypocotyl and cotyledon segments as well as shoot tip explants were dissected from seedlings. The explants were then cultured on MS medium supplemented with IAA, GA₃, NAA, and BAP and their response to this media was studied. A completely randomized design with different replicates was used to conduct the experiments. Callus was formed at the base of the hypocotyl explants on the medium containing 15 mg.l^-1 IAA, and 3.4 mg.l^-1 GA₃. Calli capable of regeneration was obtained after subculturing on the medium containing 2 mg.l^-1 IAA, and 3.4 mg.l^-1 GA₃ at the rate of 38 percent. The vitrified plantlets were abnormally glassy, and translucent which might have high water content. It was a physiological disorder which was overcome when an improved agar medium raising to 9 gl^-1 was used. Callus has been obtained only from the hypocotyl explants, while regeneration has been obtained from shoot tip cultured on the medium containing 0.02 mgl^-1 BAP at the rate of 80 percent.

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Date palm (Phoenix dactylifera L.) is propagated traditionally through offshoots or suckers, which usually appear at or below the ground level surrounding the stem base. However, there are many problems associated with this system. Offshoots are produced in limited number and vegetative propagation through them is slow, laborious, time consuming and expensive. The present study was conducted to determine the best micropropagation protocol for date palm in Kebab, Estameran, Piarom, and Berehi cultivars. The shoot apical meristem from two to three-year-old offshoots was used as source of explants. They were cultured in callus initiation medium, containing different concentrations of 2,4-D (40, 60, 80 and 100 mgl-1), NAA (10 and 20 mgl-1) and 2ip (3 and 5 mgl-1). All cultivars produced high percentage of callus with good quality in a matter of callus friability and color in 100 mgL-1 2,4-D, 20 mgL-1 NAA, and 3 mgL-1 2ip. Kabkab cultivar was superior for callus production (87.25%) in comparison with other cultivars. The calli were then transferred to a proliferation medium and then transferred to somatic embryogenesis medium containing different concentrations of Kinetin (2, 4 and 6 mgL-1), BAP (2, 4 and 6 mgL-1), and NAA (0.1, 0.5 and 1 mgL-1). Somatic embryogenesis was observed in MS medium supplemented with 2 mgL-1 kinetin, 2 mgL-1 BAP, and 0.1 mgL-1 NAA. Kabkab and Estameran cultivars showed higher somatic embryogenesis in comparison with other two cultivars. The somatic embryos were then transferred to MS medium without hormones under light, where they produced shoots and roots. Abbriviations: 2,4-D- 2,4-dichlorophenoxiaceticacid 2ip-N6(2-isopentenyl)adenine NAA-Naphthalene acetic Acid BAP-6-Benzylaminopurine MS-Murashige and skoog (1962).

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Somatic embryogenesis is affected by several factors. In this research project, we studied the effect of explant size, wounding and desiccation treatments on somatic embryogenesis and their conversion into plantlet among three genotypes of soybean. The explants were sampled from immature embryos of soybean in three different sizes (3, 5 & 7 mm) with wounding treatment on half of each, and then were cultured on the somatic embryogenesis medium. In order to determine desiccation effect on conversion amount of embryos into plantlets, the produced embryos were affected by three levels of desiccation treatments (2, 4 & 6 days). The increase ratio of callus mean weight, percentage of embryogenic calli, embryo number per explant and percentage of embryo conversion to plantlet were used for treatment evaluation. Variance analysis of the data showed significant differences (P<0.01) between treatments regarding the variables. The results indicated that BP was a superior genotype with embryogenic capability (24.19 %) and the best explant size for somatic embryogenesis was immature embryo with 3 mm length. The six day desiccation treatment caused highest percentage of embryo conversion into plantlet (74.7 %). Wounding increased callus production on explants and number of embryos per explant (20.28), but it did not show any significant effect on percentage of embryogenic calli. Germinated somatic embryos were transferred to pots containing peat-moss. Somatic embryogenesis is an efficient method for the plant regeneration and genetic transformation. However, this method still offers low percentages of plant regeneration, and is perhaps related to the maturation process and high morphological abnormalities of the matured embryos. This study aimed to find some solutions for soybean somatic embryogenesis problems.

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Geologic and pedologic controls are the main factors determining the behavior of elements in natural soil environments. In order to assess the role of these factors on content and distribution of selected major and trace elements in soil, six parent materials including: phyllite, tonalite, periditite, dolerite, shale and limestone were selected in Fuman-Masule region. Soil genesis and development of representive residual pedons were studied for each parent material and the total content of Si, Al, Ca, Mg, Fe, Ti, Mn, Ni, Co, Cr, Cu, Pb, V and Zn were compared among them. Enrichment/depletion patters of trace elements were assessed using Ti as reference element. Generally, Cr, Ni, Co and V are highest in soils derived from peridotite (984, 285, 53 and 204 mg/kg, respectively) and dolerite (1023, 176, 39 and 185 mg/kg, respectively). In the same way, Si and Al exhibit the features of parent materials in the sense that the lowest content was observed in soils developed on peridotite, dolerite and limestone. Zinc and Pb are highest in soils derived from shale (106 and 27 mg/kg, respectively). In a given pedon, different elements exhibited different enrichment/depletion patterns moreover, a given element may behave differently not only in soils with different parent materials but also, in some cases, in soils developed on similar lithology. Lead, Zn, Cu and Mn have been generally enriched in most pedons, except in some acidic and strongly leached soils, whereas Co, Cr, Fe, Ni and V have been leached, especially from Dystrudepts and Eutrudepts. The latter elements, however, showed enrichment trend in Hapludalfs and Argiudolls parallel to the development of illuvial B horizons.

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Geologic and pedologic controls are the main factors determining the distribution of elements in natural soil environments. In order to assess the role of these factors in the content and distribution of major elements of soil, six parent materials including phyllite (Ph), tonalite (To), periditite (Pe), dolerite (Do), shale (Sh) and limestone (Li) were selected in Fuman-Masule region. Soil genesis and development of representive residual pedons were studied for each parent material. Total content of Si, Al, Ca, Mg, Fe, Mn, K, Na, Ti and P of soil horizons were measured and compared to the geochemical and mineralogical composition of parent materials. Maximum concentrations of Fe2O3 and MgO were found in the soils derived from Pe and Do however, these soils had low content of SiO2 and Al2O3, which is in conformity with the geochemical composition of the parent rocks. On the contrary, FeCBD content of these soils was lowest, indicating the low degree of soil development and, by the same fact, the importance of inheritance factor in soil Fe concentration. However, comparison of total Fe and FeCBD in Li1, Sh2 and To2 revealed that relative development of these pedons is higher than the others. Silicon depletion in Ph1, To2 and Sh2 pedons, relative to parent rocks, is higher than in Pe and Do pedons. However, this element is enriched in Li pedons. MnO content of Pe and Do pedons is governed by geogenic factors, while in Sh pedons, pedogenic factors, especially redox conditions, play the major role. Exchangeable forms of Ca and Na are determined by soil properties rather than by parent material type. Notwithstanding the redistribution of all major elements throughout pedons due to soil forming processes, the importance of inheritance factor in soil Si, Al, Mg, Fe, K, and Ti is higher than pedogenic factors.

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In order to study land degradation from the soil genesis and micromorphological perspective, ten soil profiles were dug and described on five slope positions in both forest and deforested cultivated land. The soil samples were taken from all horizons for physico-chemical analysis and micromorphological studies. Forest soils had a well developed argillic and calcic horizons and also mollic epipedon. These soils were classified as Alfisols and Mollisols. Carbonate leaching into the depth of soil profile and translocation of clays to lower layers and formation of developed soils are by no means related closely with the dense forest cover and its subsequent landscape stability and favorable leaching conditions. Dominant soil orders in this area were Inceptisols and Mollisols. In cultivated landuse, no argillic horizon was observed except in toeslope position. Absence of argillic horizon or its elimination following deforestation is one of the most important and obvious pedogenetic evidences of land degradation after land use change. Outcropping of high carbonatic layers (calcic horizon), disappearance of mollic and formation of ochric epipedon, presence of redoximorphic features attributed to runoff in lowland, decrease of solum thickness, and change of soil color were the other pedogenic indicators for land degradation in the study area. Microscopic observations showed that granular and crumb microstructures with high porosity were converted to massive and compact ones with low porosity in the deforested area. Disappearance of clay coatings besides absence of excremental pedofeatures were the other important micromorphological evidences of erosion and land degradation.

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The aim of this study was to evaluate soil properties changes in a soil toposequence series in Khuzestan province. Twelve soil profiles were dug in north to south direction in this province, according to the changes of the slope in Karoon’s alluvial deposits. The results showed that soil texture regularly changes to heavier form from north to south. It was slightly of redoxomorphic features in low land. Therefore, salic horizon was formed in this land. Despite high water table in this area, gray or mottle forms were few due to high soil salinity. The carbonates were eluviated from surface to subsurface horizon therefore, calsic horizon was formed due to illuviation. Secondary carbonate accumulation was maximum in the studied area. Both classic horizon and cambic horizon were formed in some pedons under long time cultivation in north regions. These developments were originated from organisms that grew under good drainage class. The smectite was seen in the south part of region. It may have been transported by river and deposited under high salinity and flocculated in contact to Persian Gulf’s saline water. The palygorskite was not seen in all patterns. This clay was removed in pedons under long time cultivation in the north area due to more leaching. High irrigation water can accelerate palygorskite transformation. Also, the XRD patterns showed that chlorite, illite, kaolinite and quartz were main clay minerals in the studied soils.