Showing 11 results for Genetic Variation
Farhad Ghavami, Abdolmajid Rezai, Siroos Abdemishani, Ahmad Arzani,
Volume 3, Issue 2 (7-1999)
Abstract
Variability of seed storage protein electrophoretic patterns revealed by SDS-Polyacrylamide gel electrophoresis and their possible relations with some morphological and phenological characteristics were studied using 193 accessions from Iran Mungbean (Vigna radiata L. Wilczek) Collection. Seed protein electrophoresis revealed six different patterns that were different in two albumin and four globulin subunits with molecular weights of 23500-34000. Pattern types 1 and 2 had a considerable frequency but pattern types 3, 4, 5 and 6 had low frequencies and probably had developed recently in mungbean evolution process. Cluster analysis of countries and cities using the frequencies of albumin and globulin subunits showed no relation between geographical diversity and similarity distances. The correlations of morphological traits and albumin and globulin subunits indicated a relationship between G1 and G2 subunits with 1000 seed weight and days to beginning of maturity. Therefore, selection based on protein patterns at preliminary stages of breeding programs might be effective in increasing seed yield together with earliness.
M. Azimi, S. Massiha, M. Moghaddam, M. Valizadeh,
Volume 3, Issue 4 (1-2000)
Abstract
In order to study the genetic variation among local varieties of onion in Iran, an experiment was conducted in the Research Center, Faculty of Agriculture, Tabriz University. Sixteen populations were evaluated for agronomic characteristics and also total seed proteins via SDS-PAGE. Cluster analysis and principal component analysis were used to group the onion populations under study.
Analysis of variance showed significant differences among varieties for leaf color, leaf length, texture tightness, onion yield per plant, and number of edible layers. No significant differences were observed for the number of twin onions, bulb diameter, and onion dry weight. Hamadan (98-148), Arak (98-95, 98-96, 98-97, 98-103), and Zanjan (98-223) populations acquired the highest onion yield per plant. The significant differences between populations for the majority of characteristics proved the existence of genetic variation in the Iranian onion germplasm. The results from cluster analysis for agronomic characteristics were the same as those from the cluster analysis for the onion yield per plant. The 16 populations were divided into 4 groups. Cluster analysis for the electrophoresis banding pattern resulted in two groups, which was not similar to the dendrogram of agronomic traits. Using principal component analysis, the first principal components determined 97.57% of the total variation. Onion yield per plant was the most important trait in the first principal component and onion dry weight was the second trait in the rank.
A. Dehdari, A. Rezai, M. Mobli,
Volume 5, Issue 2 (7-2001)
Abstract
In order to evaluate the morphological and agronomic characteristics of 19 land races of onions and one foreign cultivar (Yellow Sweet Spanish), an experiment was conducted in 1998 at the Research Farm of the College of Agriculture, Isfahan University of Technology.
Analysis of variance showed significant differences among genotypes for all of the morphological and agronomic traits. Dry weight and number of days to emergence had the highest and lowest coefficients of genetic variability, respectively. Plant fresh weight, yield of 30 plants and total yield also had high coefficients of variability. Broad sense heritability estimates were high for all of the traits, indicating low environmental affects them. Based on cluster analysis and plot of the first two canonical variables, the genotypes were classified in four groups with different agronomic traits. Canonical discriminant analysis based on nine agronomic traits introduced three canonical variables which justified 99.9 percent of the total variation among characters. Principal component analysis revealed four components while factor analysis showed three factors which explained 87.3 and 95.5 percent of the total variation among characters, respectively. The first and second factors were related to adaptation and assimilate translocation, respectively. Bulb diameter, bulb height and bulb weight in positive directions and sensitivity to Fusarium in negative direction had greater loads in the third factor.
G. Saeidi,
Volume 5, Issue 4 (1-2002)
Abstract
Flax (Linum usitatissimum L.) is an oilseed and widely adapted crop. Oil of regular genotypes of flax is used in industry because of its unique fatty acid profile. New, mutant genotypes of flax have oils similar to sunflower oil which can be used as edible oil. This experiment was conducted to investigate the genetic variation of agronomic traits and productivity in different edible and industrial oil genotypes of flax in Isfahan. The genotypes were evaluated in augmented design.
Based on the results, the average numbers of seedling/m2 in edible and industrial oil genotypes were 178 and 367 with variation coefficients of 70 and 10%, respectively. Maturity also showed variation and varied between 89 to 116 days in edible oil genotypes and between 89 to 128 days in industrial oil genotypes. Plant height varied between 57 to 86 and 49 to 73 cm in edible and industrial oil genotypes, respectively. Seed yield also had considerable variations and varied between 429 to 2651 and 779 to 2389 kg/ha with variation coefficients of 35 and 25 in edible and industrial oil genotypes, respectively. Seed yield per plant showed a high and positive correlation with basal branches (r=0.77**) and bolls per plant (r=0.93**), but high and negative correlation with stand (r=-0.66**). Regression analysis revealed that approximately 96% of the variation in seed yield per plant was attributed to variation in bolls per plant, seeds per boll and seed weight and they were determined as the major yield components, respectively. Bolls per plant was the most important yield component and contributed to 87% of the variation for seed yield per plant.
T. Najafi Mirak, A. Zali, A. Hossainzadeh, Gh. R. Rasoulian, A. Saeidi,
Volume 7, Issue 4 (1-2004)
Abstract
Russian Wheat Aphid (RWA) is a serious pest of wheat, barely and other small grains in cereal-producing regions of the world. The use of resistant cultivars is an effective management strategy to control this pest. In this study, 15 lines and 13 cultivars of durum and bread wheats along with two resistant and susceptible controls were used to evaluate their reaction to RWA under greenhouse conditions, using completely randomized design with four replications. Three seedlings at each plot were considered as an experimental unit. Five aphid nymphs (4-5 ages) were placed on each plant at 1-2 leaf stage. Leaf rolling and leaf chlorosis percentages were measured 21 days after infesting and used to screen the genotypes. Despite the relatively high linear correlation between two resistance components, leaf rolling and leaf chlorosis, estimated as 80%, analysis of variance indicated significant differences among genotypes. Eight durum lines DW9, DW14, DW4, DW2, DW7, DW8, DW12 and DW3 and one bread wheat cultivar (Azadi) were significantly more resistant than the resistant control (Halt). One durum line (DW13) and two bread wheat cultivars (Shiroodi and M-75-7) had higher leaf rolling and leaf chlorosis than the susceptible control (Sholeh). So they were selected as the most susceptible to the biotype of D. noxia. under study. Orthogonal comparison showed that durum lines were more resistant than bread wheat cultivars. These results were confirmed by Cluster Analysis based on leaf rolling and leaf chlorosis.
M. Farshadfar, E. Farshadfar,
Volume 8, Issue 2 (7-2004)
Abstract
Agropyron is one of the most resistant plants to biotic and abiotic stresses. It plays an important role in forage yield in rangelands. Genetic variability based on different markers is the important step in crop improvement. In order to evaluate the genetic variation of different Agropyron species based on morphological and chemical traits, this experiment was carried out in the Agriculture and Natural Resources Research Center of Kermanshah. The tiller numbers, spike length, spikelet numbers, length of flag leaf, width of flag leaf, plant height, peduncle length, ash percentage, organic matter, fiber, dry matter, fat and crude protein were registered. Statistical analysis of data was done by SPSS software. According to cluster analysis the tetraploid genotypes were classified into 5 clusters. Based on principal components analysis the length of flag leaf, spike length and plant height, and among the chemical traits of the ash percentage, organic matter, and crude protein had the highest portions of variance. The genetic parameters such as PCV, GCV, ECV, Hb and Ga for length of flag leaf were 0.274, 0.169, 0.215, 0.382, 3.05 and for spike length were 30.96, 21.64,22.139, 0.48, 5.786 and for plant height were 0.16, 0.084, 0.136,0.276, 6.054, respectively.
F. Rafeie, G. Saeidi,
Volume 9, Issue 2 (7-2005)
Abstract
To study the genetic variation of different traits, 66 isolated lines from different Iranian safflower landraces of Isfahan, Azarbaijan, Khorasan, Kordestan, Central provinces along with 13 foreign genotypes and two local populations of Kooseh and Arak-2811 were evaluated in a simple lattice design with two replications. The experiment was conducted at the research farm, Isfahan University of Technology in the year 2001.
The results indicated that there was significant differences among the genotypes for all the traits including days to 50% flowering, maturity, plant height, yield/plant, seed yield and its components and relative resistance to the powdery mildew disease (p<0.01). Seed yield of genotypes varied from 1285 to 3524 Kg/ha. The seed yield of local population of Kooseh which was dominant growing cultivar in Isfahan province and one of the latest in maturity and tallest genotype was 2317 Kg/ha, however, some genotypes were earlier in maturity and had the less plant height and more seed yield than Kooseh. Seed oil content was measured for the 20 high yielding isolated lines by the Soxhlet method and varied from 24.62% (in one of the isolated lines from Kordestan landrace) to 37.55% (in one of the isolated lines from Kooseh). The genotype of Kooseh had seed oil content of 35.99%. Based on the results of cluster analysis, the genotypes were classified in to 3 the distinct clusters and they were significantly different for all of the traits, except for days to 50% emergence. One of the clusters had the maximum seed yield, seed yield/plant, branches/plant, capitula/plant, seeds/capitulum and the minimum of seed weight. It was implied that the genotypes of this cluster which were isolated from local populations could be used for improving the seed yield. The cluster analysis also indicated that in isolated lines from different provinces, there was no special pattern between the genetic and geographical diversity.
E. Farahani, A. Arzani,
Volume 10, Issue 4 (1-2007)
Abstract
An experiment was conducted to investigate the genetic diversity in the cultivars and F1 hybrids of durum wheat, using agronomic and morphological traits. Evaluation of consistency between choosing parents of crosses based on morphological and agronomic observations and genetic distance obtained from cluster analysis were objectives of this study. Fourty- two genotypes were evaluated using a randomized complete block design with three replications in 2003. Agronomic characteristics comprised days to 50% flowering, days to 50% pollination, days to maturity, plant height (cm), spike length (cm), grain weight per spike (gr), number of grain per spike, number of spike per m2, grain yield (t/ha), biological yield (t/ha), 1000 grain weight, test weight (gr/L) and harvest index were measured in the studied genotypes. The results of analysis of variance showed significant differences among genotypes for all the studied traits. The results also revealed that grain yield had the highest coefficient of variation (CV= 21.6%), followed by spikes per m2 (CV= 20%) and number of grains per spike (CV= 18.9%). Lowest coefficient of variation was observed in days to maturity, days to 50% pollination and days to 50% flowering. Moreover, correlation analysis indicated that grain yield correlated with harvest index, biological yield, grain per spike and grain weight per spike. Cluster analysis of genotypes based on agronomic traits defined desirable clusters. These clusters have desirable characteristics and are beneficial for breeding objectives. Results of evaluation of consistency between choosing parents based on observation and those of cluster analysis showed varing degrees of consistency in 12 conducted crosses, ranging from highly similar parents in Eupoda6×Chahba88 to relatively distinct parents in PI40100×PI40099 cross.
M. H. Banabazi, S. Esmaeilkhanian, S. R. Miraei Ashtiani, M. Moradi Shahrbabak,
Volume 10, Issue 4 (1-2007)
Abstract
Genetic variation within and between five Iranian sheep populations including Sanjabi (SAN), Kordi Kordistan (KKO), Kordi Khorasan (KKH), Mehraban (MEH) and Moghani (MOG) was assessed using six microsatellite markers (McMA2, McMA26, MAF64, OarAE64, OarCP26 and OarFCB304). The PCR reactions were successfully perfomed with all primers except OarAE64. All locus-population combinations were at Hardy-Weinberg equilibrium except McMA2 in MOG population (P<0.005). Polymorphism criteria showed that the five studied loci were polymorphic in all populations. The lowest DA genetic distance (0.234) was observed between KKH and KKO and the highest (0.388) between SAN and MOG populations. The dendrograms based on DA distances were drawn using unweighted pair-group method using an arithmetic average (UPGMA) and neighbor-joining (NJ) method. KKO, KKH and SAN were grouped together at one cluster and MEH and MOG at another by both methods. The average expected heterozygosity for each populations (as interpopulation variation) ranged from 0.744 to 0.847 for KKH and MEH, respectively. The estimated time of divergence for two Kordi populations (KKO and KKH) was 445 years that complies with historical evidences. The findings of this research confirmed that microsatellite variation could be a useful tool for screening of investigating biodiversity among domestic animals.
A.r. Khanahmadi, Gh. Rahimi, A. Nejati-Javaremi, S. Esmaeilkhanian,
Volume 11, Issue 40 (7-2007)
Abstract
In order to detect genetic variation of native fowls in Mazandaran native Fowls breeding station, blood samples were collected from 100 male and female of birds (1:11). The DNA of the blood samples was extracted according to an optimized salting out protocol. The extracted DNA was amplified through polymerase chain reaction (PCR). Of the twenty random primers (10 mer) were used in this study, fourteen yielded satisfactory PCR. The total 63 polymorphic and 77 monomorphic bands were detected for the 14 primers. The number of bands displayed for each primer ranged from 4 to 16 with 200-2100 base pairs. The highest and lowest percentages of polymorphism band were observed for primer 9 (72%) and primer 14 (16%) respectively. The band sharing frequency was calculated for each primer, which ranged from 79 to 96. The genetic similarity within population and genetic variation were estimated as 89 and 11 percentage respectively. In conclusion, the existence of high level of polymorphism after ten generation of selection may indicate the accuracy of genetic evaluation program, suitable selection strategies and also large enough effective population size in this breeding flock.
M. Golestani, H. Pakniyat,
Volume 11, Issue 41 (10-2007)
Abstract
To investigate genetic variation, and identification of tolerant genotypes according to quantitative indices of drought tolerance, 8 sesame genotypes were tested in a randomized complete block design with three replications under optimum and limited irrigation at the Research Station of College of Agriculture, Shiraz University. Based on the potential yield and yield under stress, quantitative indices of drought tolerance such as mean productivity (MP), geometric mean productivity (GMP), harmonic mean (HM), stress tolerance index (STI), stress susceptibility index (SSI) and tolerance index (TOL) were determined. The result of analysis of variance exhibited highly significant differences among the genotypes for all the indices measured, and yield under optimum and limited irrigation, indicating the existence of genetic variation among genotypes, and thus the possibility of selecting drought tolerant genotypes. Mean comparison displayed that the highest potential yield, stress yield, MP, GMP, HM and STI were related to the genotype number 5. Correlation analysis between indices, mean potential and stress yields indicated that every four indices are suitable for screening the genotypes. Based on these indices and higher yields under optimum and limited irrigation the best drought tolerant genotypes were identified to be genotypes number 4 and 5. Multivariate biplot indicated that the genotypes number 4 and 5 were located next to the vectors of drought tolerance indices, including MP, GMP, HM and STI. Cluster analysis showed genetic distance among genotypes. As a result, genotypes number 4 and 5 were identified as drought tolerant and genotypes number 1, 2 and 3 as susceptible to drought.
