JWSS - Journal of Water and Soil Science

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In this study, an experiment was carried out to determine the effects of 6 culture media (sand, perlite, vermiculite, Sand + Perlite, Sand + Vermiculite, Vermiculite + Perlite) on the rooting of single-bud cuttings of seven grape cultivars (Rishbaba, Hosseiny, Dashgarah, Ghiziluzum, Teberzeh, Bidaneh Sefid, Sahebi). All the cuttings were prepared from one-year old branches. Statistical analyses of the data obtained from the examination of the roots revealed significant discrepancies in the rooting of different cultivars' cuttings, media and media-cultivar interactions in terms of rooting percentage, fresh weight of roots and the ratio of dry to fresh weight of roots. The highest rooting performance in terms of the above parameters was observed in Hosseiny cultivar while the lowest obtained in Teberzeh cultivar. Vermiculite and perlite + Vermiculite were found to be the most suitable media for rooting of cuttings. According to the media-cultivar interactions, high rooting of Hosseiny, Dashgarah, and Ghiziluzum was observed in vermiculite and high rooting of Rishbaba, Teberzeh, Bidaneh Sefid and Sahebi was obtained in perlite + vermiculite.

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The effects of four levels of SO₂ (0.0%, 0.06%, 0.125% and 0.25%) on the qualitative and quantitative characteristics of two Persian grape cultivars “Keshmeshi seedless” and “Shahroudy” were evaluated during the cold storage in 1995-1997. This research was carried out in a factorial experiment in a completely randomized design in cold storage at the laboratory of Horticultural Department, College of Agriculture, Tehran University.

The results showed that the effect of SO₂ on decay was significant at 1%, and maximum control was obtained at 0.25%. No significant difference was observed when treated with 0.125% SO₂ However, increasing SO₂ concentration resulted in increased quantity and intensity of bleaching on the berries. At these increased concentrations, a linear relation was observed between bleaching on the berries and SO₂ concentration so that the highest bleaching occurred at a concentration of 0.25%. Increased storage periods also increased bleaching. The effect of SO₂ on weight loss, shattering, and rachis colour was also significant, decreasing rachis colour and shattering of berries.

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Populations of Arboridia kermanshah Dlabola is increasing in Isfahan vineyards. In this study, some morphological characteristics of the immature stages, over wintering sites, number of generations per year, and seasonal population fluctuations of this pest were studied in vineyards in Isfahan Province. Eggs showed apparently four distinct embryonic developmental stages. At 25.8 ±2˚C, development of eggs was completed in 10.9 ± 0.7 days. There were five nymphal developmental stages. Nymphal stages can be distinguished with wing pad development and width of head capsule and width of pronotum. The grape leafhopper overwintered as adults under leaves and crop residues. Rearing studies showed that the grape leafhopper had three generations per year, each lasting 33.9±0.6 (without overwintering period), 46.3±0.7 and 37.3±0.6 days, respectively. Seasonal monitoring of adults, nymphs and eggs showed three or four overlapping periods of activity in the field. Four periods of activities of adults were recorded through season from early May – mid June, mid June – mid July, mid July - late August and early September – mid October.

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A 2-year vineyard survey failed to the provide evidence that Uncinula necator (Schw.) Burr. survived winter as mycelium in dormant infected buds in Khorassan province. Ascospores of U. necator, were collected by a volumetric spore trap operating constantly in a vineyard for 55 days after the bud burst. The first powdery mildew colonies were consistently found on the leaves of the shoots (7.30 cm long) growing on the vine. Cleistothecia were found on all plant parts infected during the previous growing season. More than 35-45 % of the cleistothecia borne on the leaves and stems died during winter. Most of the ascospore discharge occurred between the bud burst and the blooming time. Ascospores were periodically released from cleistothecia on the leaves kept in vineyard from October to May, while the ascospores germinating on the glass slides germinated from October to January and then germination slowed down to zero in early March and the water content potential of ascospore cytoplasm decreased constantly during this period as well. The mass required to fracture the ascocarp wall during maturation was measured to be approximately 5g in autumn, 3g in winter and 2.5 g in early spring. The most rapid decrease in the strength of the ascocarp wall occurred during a 4-week period before dehiscence in the field. The effect of the pathogenic property ascospores on healthy leaves demonstrated their role as a primary inoculum source. Cleistothecia appear to be the principal means of overwintering of U. necator in Khorassan province vineyards.

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Some bioecological aspects of grape leafhopper, Arboridia kermanshah Dlabola, including: the egg distribution under the leaf surface, eggs and nymphs spatial distribution on the vine arms, the adult distribution on three strata of the vine canopy, the adult diel activities and the geographical distribution of this species in Isfahan province were studied. Samplings were done in the 1997. The egg distribution under the leaf surface was determined by repeated sampling during the season and the egg density was estimated on three regions such as the main and secondary vein, the leaf surface margins and others. Eggs and nymphs spatial distribution along the vine arms were studied weekly for six weeks by sampling an arm of five vines during the second generation, the leafhopper population was the highest. The adult spatial distribution on the vine canopy was determined by sampling three strata of the vine canopy using the D-vac apparatus. Diel activities were studied by sweeping nets fifty times per vineyard at the two hour intervals from 8 a.m. to 20 p.m. Temperature and the relative humidity were also measured in the shade once per hour. The presence and the abundance of this species in 87 vineyards in the different climatic conditions were studied by a sweeping net. The leaf margin and around of the secondary vein were preferred for the oviposition of the leafhopper. Adults, nymphs and eggs were more abundant in the middle strata of the vine canopy than others. The mean pattern of the daily activity of the adult indicated one peak at 8 a.m. and the other one at 20 p.m. Maximum temperature thereshold for the adult activities was 28º C. The study of the grape leafhopper distribution in isfahan province indicated that this species was distributed in the most vineyards, but was highly abundant in more humid conditions.

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In 1997, in two vineyards of Isfahan university of technology and Zobeahan in Isfahan, the density fluctuations of Arboridia kermanshah Dlabola and their injury symptoms were studied. In each vineyard three leaves of each of ten randomly selected vines were sampled weekly. Nymphs in different instars were counted directly and rate of injury symptom (leaf discoloration) was determined with planimeter. Special cages were used to determine rate of injury symptom for each nymphal instar in comparison to each other. Rate of injury symptoms and nymphal density indicated two peaks through growing season until harvest. Tolerance threshold for A. kermanshah nymphs was determined 80 first instar nymphal days or presence of 11-12 first instar nymphs and feeding for seven days. With increase of nymph densities, damage symptom increased rapidly.

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The aim of the study was to examine the effect of CaCl₂ on the firmness and post harvest retention of Berry Grape (Vitis vinifera L.) cv. Askari. The experiment was carried out in 2006 in vineyard in the Sisakht region in a completely randomized design with 7 treatments. Experiment includes two parts: 1- spray with 3 levels 0.1, 0.25 and 0.5 %. (Two weeks before harvest and repeated one day before harvest) and 2- Dipping clusters in CaCl2 solution with 3 levels 1, 2.5 and 5 % post harvest. Grapes were stored at 0ºc for 1 month in cold store room. Results showed that the effect of CaCl₂ was significant on post harvest abscission berries percentage at 1% level. Minimum post harvest abscission of berries percentage was observed using 5% dipping and 0.25% spray after 30 days storage in cold storage room. Highest abscission of berries was observed in control. Effect of CaCl₂ on decay berry was significant at 1% level after 30 days of storage. Minimum decay percentage was observed in 0.25 % spray and the highest decay percentage was obtained in control. Effect of CaCl₂ on palatability (TSS/TA) was significant at 1%level.

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To study the effects of different combinations of auxin (IBA, NAA) and cytokinin (BAP, TDZ) regulators, basal salts (MS, WPM, NN) and gelling agents (Gelrite and Plant Agar) on the growth and development of seedless grape explants (cv. Bidaneh Soltani), a factorial experiment was conducted based on a completely randomized design. The number and length of grown shoots were analyzed as two main characteristics. Growth regulators and salts had significant effects (at %1) on the shoot proliferation. The highest number of shoots was observed on MS salts combined with BAP (2.2 mg/l) and IBA (0.5 mg/l) on the 18th days of culture. Compared to Plant Agar, Gelrite had a better effect on the normal growth of the shoots. Based on the new shoots, combinations of MS salts and hormones may be used for micropropagation of seedless grape cultivars in the in vitro condition.

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Resveratrol (5,3,4'- trihyroxystilbene) is a valuable polyphenolic compound which prevents cancer, heart and vascular diseases in human. In this research, the compound was extracted from leaf and fruit tissues of two Iranian grape cultivars (Rajabie Sefide Shiraz and Keshmeshie Ghermez) using a general extraction method with some modification, and analyzed quantitatively by High Performance Liquid Chromatography (HPLC). Resveratrol content in leaf and fruit of Rajabie Sefide Shiraz cultivar was higher than Keshmeshie Ghermez and its amount in the fruits of both cultivars was observed to be greater than their leaves. The accumulation of Resveratrol at the early stage of fruit growth (4 weeks after full bloom) was higher than the subsequent stages of fruit growth and development (8 weeks after full bloom and ripened fruit) in both cultivars. This compound gradually decreased from the beginning of fruit set to the complete stage of growth and development and reached the lowest level when the fruits were ripe. Leaves and fruits of both cultivars were treated with methyl jasmonate (1 mM), then the rate of resveratrol was measured after treatments. It was observed that the amount of this compound was significantly increased in treated samples when compared with the control however, the difference diminished later. The fruits of both cultivars responded better to the treatments 4 weeks after full bloom.

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Seedlessness is the most important characteristic of fruit quality for raisin and table grapes. Grape breeding at University of Tehran, Karaj branch, Iran started in 1999 with crossing some selected seedless and seeded cultivars at the end of evaluation of 90 cultivars in grapevine collection of College of Agriculture in Karaj. Out of 1400 progeny obtained from 26 different crosses, 381 of them which produced fruits were evaluated during growing seasons of 2006 & 2007. Progenies were divided into four groups of completely seedless, semi-seedless, semi-seeded and completely seeded, according to seed trace or seed weight. Results classified progeny to 11% completely seedless, 13.6% semi-seedless, 24% semi seeded and 51.2% completely seeded. Percentages of seedless progeny for four male parents of Askary, Yaghooti, Bidane Sefid and Bidane Ghermez were 15.4%, 10.8%, 9.3% and 10.6%, respectively. Meanwhile, percentages of seedless progeny for female parents of Muscut of Hamburg, Ghezel Uzum, Dizmary, Rajabi Sefied, Ali Baba, Alhaghi Ghermez and Tabariz were 5.4%, 5%, 17.5%, 13.2 %, 1.4%, zero and 36%, respectively. Results also showed that among male parents, Yaghooti and Bidane Ghermez cultivars and among female parents, Tabarze cultivar had better backgrounds to transmit stenospermocarpic seedlessness characteristics.

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Grapevine (Vitis vinifera L.) is a clonally propagated major fruit crop. In grapevine, identification of genotypes with amplographical features is often based on mature plant characteristics that may be affected by environmental conditions. This approach lacks objectivity and reliability. Recently, molecular markers have proved to be supplementary techniques to analyze genetic diversity and examine genetic relationships existing between cultivars in a range of horticultural crops. In this study, twenty genotypes from grapevine (V.vinifera species) grown in Isfahan province were characterized by RAPD technique to understand the extent of diversity and relatedness. Fifty random primers were used for the RAPD study. Of those, twenty four informative primers which generated reproducible polymorphic bands were used for grouping the genotypes. PCR products of the genotypes’genome revealed a total of 315 bands, out of which 282 were found to be polymorphic. Average number of 13 bands was obtained per primer and the amplification produced ranged in size from 300 bp to 3000 bp. The dendrogram constructed using UPGMA cluster analysis differentiated the genotypes into two major clusters, nineteen in one group and Madar-o-Bache genotype has been placed in a separate one, indicating its high genetic diversity compared to the rest of the genotypes. Intra-clustering within cluster A grouped the genotypes in four sub-clusters as expected from their genetic background. The results of the study revealed that the RAPD technique is a relevant technique to determine genetic diversity, genomic analysis and to examine genetic relationship in grapevines.

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Overcroping, shading and short growing season can reduce the pool of carbohydrate reserves, and consequently reduce the quality of crop in grapevines. An experiment was conducted in order to study the effects of cluster number and different levels of cane topping on balanced yield and qualitative traits of table grape (Vitis vinifera L.) cv. Askari. The design of experiment was factorial in completely randomized blocks with four replications. Factors included cluster number (Control, 25, 30, 35, 40, 45 and 50 cluster per vine) and cane topping included (Control, removal 1/4, 1/3 and 1/2 length of shoot). Results showed that decreasing the number of cluster to 25-35 per vine, significantly increased %TSS, TSS/TA and pH but with increasing cluster number, these traits were significantly reduced. Thinning treatment to 25 cluster showed significant difference from the viewpoint of %TA compared with untreated control. Also topping treatment significantly increased %TSS, %TA, TSS/TA ratio and decreased % TA compared with untreated control. Reducing the cluster to 25-45 per vine significantly increased cluster weight, however, no significant difference was observed between control and 50 clusters per vine and/or 25 to 50 clusters. Yield per vine was reduced in all treatments in comparison with untreated control. However, no difference was shown between 25 to 35 and or 40 and 50 clusters per vine. According to the results obtained, cluster thinning combined with topping can be recommended for increasing fruit quality and balanced yield of Askari table grapes in cold climate of Cisakht region.

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