Showing 3 results for Hplc.
S. Saboory Halestani, S.h. Dokhani, G.h. Kabir, R. Shokrani,
Volume 4, Issue 4 (1-2001)
Abstract
Four cultivars of olive, Kalamata, Marri, Zard and Fishmi were obtained from Roodbar town and treated with two methods, natural and controlled fermentation with Lactobacillus plantarum as a starter culture at 25°C for 100 days. HPLC analysis experiments were conducted during the whole study period. A higher level (1.08% w/w) of total acidity was produced in Fishmi cultivar especially in the natural fermentation (0.8-1.1 g lactic acid/ml) than in other cultivars. Individual organic acids were determined by HPLC. Quantitative changes of organic acids in olives showed (P<0.0l) an increasing pattern of lactic and acetic acids during fermentation, but decreasing levels of malic and citric acids were revealed. The highest level of lactic acid (1.1% w/v) was observed in Zard cultivar and the type of fermentation had no effect on acid production. Malic and citric acids gradually disappeared during 50 days of fermentation period and this change was faster for citric acid during controlled fermentation. Malic acid did not follow this pattern.
F. Khajehali, H. Nassiri Moghaddam, R. R. Marquardt, M. Danesh Mesgaran,
Volume 6, Issue 3 (10-2002)
Abstract
Evaluation of uric acid contents of poultry excreta was examined for use in the estimation of apparent protein digestibility. Uric acid was determined either by high-performance liquid chromatography or by spectrophotometric method. A high-pressure liquid chromatographic assay was used with absorbance measured at 285 nm. The method used a reverse-phase system with a C18-bonded column (250*4.6 mm, i.d.). The mobile phase was distilled deionized water. Injection volume was 20 l with a flow rate of 1 ml/min.
Correlation (r) between the two methods for uric acid content of excreta from birds fed diets with different methionine levels was 0.976. The uric acid values were then used to determine apparent protein digestibility. The apparent protein digestibility values, when corrected for uric acid nitrogen, were comparable with those calculated from amino acid analysis.
Z Davarpanah, M Shey Zeinodin, Sh Dokhani, Gh Saeedi,
Volume 13, Issue 47 (4-2009)
Abstract
Sweet tasting glycyrrhizic acid is considered to be the most important chemical compound in licorice root. However, environmental factors affect chemical composition of licorice root including glycyrrhizic acid content. In this study, the effects of the harvesting time and location on licorice root composition were studied. Chemical composition of the licorice roots (Glycyrrhizia glabra) collected in summer and fall seasons from three locations in Fars province (Eghlid, Beiza and Doshman-Ziary) and one location from each of Kerman (Baft) and Kermanshah (Islam-Abad) provinces was determined. Glycyrrizhic acid, ash and sugar content before and after hydrolysis were measured in all samples after extraction. A combined analysis of variances was performed on data based on a randomized complete block design in six replications. The results showed that effects of harvesting season on sugar content, and the effects of location and its interaction with harvesting season on ash, sugar and glycyrrhizic acid content were significant (p<0.01). It was also found that the lowest ash content were obtained from roots harvested from Islam-Abad in summer the highest sugar content before hydrolysis belonged to roots harvested from both Baft and Islam-Abad in fall. Highest amount of sugar after hydrolysis was reported in the roots collected from Islam-Abad in Fall. Roots harvested from Beiza in Fars province in fall had the maximum glycyrrizhic acid content.
