JWSS - Journal of Water and Soil Science

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In order to estimate the heritability of morphological characters of worker honey bee and honey yield, 30 queen-mothers were selected randomly from the apiary of Research Center of Animal Science and Natural Resources in Isfahan Province. From each queen-mother, five queen-daughters were reared and introduced to equalize colonies after natural mating of queens. Summer honey yield was measured, while fall honey production was calculated from area of sealed honey combs. The total honey was calculated by adding summer honey production to fall honey production. Morphological characters were measured under a stereo-dissecting microscope fitted with a calibrated micrometer reticle according to Ruttners method (1985).

 Heritabilities were estimated by intraclass correlation method. Heritabilities of summer, fall and total yearly honey yields were estimated to be 0.64, 0.51 and 0.30, respectively. Estimates of heritability of morphological characters for probscis length, tibia length, femur length, metatarsus length, metatarsus width, forewing length, forewing width, cubital index, hindwing length and number of hamuli were 0.64±0.08, 0.45±0.07, 0.50±0.07, 0.47±0.07, 0.33±0.06, 0.42±0.06, 0.89±0.10, 0.5±0.07, 0.94±0.10 and 0.45±0.07, respectively. It could be concluded that in order to increase honey yield in either season (summer or fall), selection must be based on the amount of honey yield in the corresponding season. Thus, heritabilities of forewing and hindwing lengths were high selection according to these morphological characters would, therefore, change the size of these characters.

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To estimate the relationship between honey yield and some of the morphological characters, five queen-daughters from each of 30 queen-mothers were studied. The queen-mothers were selected randomly from the apiary of Research Center of Animal Science and Natural Resources in Isfahan Province. Summer honey yield was measured from the difference of honey comb before and after honey extraction while fall honey production was calculated from area of sealed honey combs. Morphological characters were recorded using a stereodissecting microscope fitted with a calibrated micrometer reticule according to Ruttner’s procedures. The mean of honey yield for a year was estimated to be 10.05 kg. Phenotypic correlations between yearly honey production and summer and fall honey productions were estimated to be 0.49 and 0.88, respectively, while the correlation between summer and fall honey yields was low (0.019). There were significant correlations between summer honey yield with cubital-b index, tibia length and forewing width (0.28, 0.27 and 0.25, respectively). Also there were significant correlations between metatarus width with total and fall honey yields (0.22 and 0.23, respectively). Genetic correlations between metatarus length and cubital index with summer honey yield were negative (-0.75 and -0.45, respectively). It may be concluded that due to the high correlation between summer honey production and total honey yield, one could improve yearly honey production by selecting on the basis of summer yield of colonies. Among morphological traits, metatarus length, forewing width and cubital index could be used in the breeding plan in order to increase honey yield.

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During the honeybee breeding project in central Iran, sex alleles homozygoty and sex alleles number in the third generation of 364 colonies were studied in 2003. Sex alleles homozygoty was measured based on Ruttner (1988) and Tarpy and Page (2002) methods. The total area of worker brood area, stored pollen, and drone brood area were measured on the combs. The whole extracted honey weight and remaining honey in the combs were evaluated as honey production of colonies. Adult population was evaluated based on the number of full combs of adult honeybees. The results showed that sex alleles, average homozygoty and number in the colonies were 18.83% and 5.32 respectively. The results showed no significant correlation between homozygoty of sex alleles or sex alleles number and stored pollen ( P > 0.05 ) , but significant negative correlation between sex alleles homozygoty and colony population, total brood area and honey yield ( P < 0.01 ) was observed. So introduction of new sire colonies in the mating Isolated area is essential to prevent sex alleles homozygoty and also performance of honeybee colonies from decreasing.