M. Rahemi, A. Haj Abdollahi,
Volume 8, Issue 1 (4-2004)
Abstract
Experiments were conducted during 1996-1997 in Nough of Rafsanjan to evaluate double pollination on fruit set and development of pistachio nut. In the first experiment, Owhadi cultivar was pollinated by a combination of pollen from Beneh (P. mutica F&M), Atlantica (P. atlantica Desf) and Soltani (P.vera L.). In the second experiment, Owhadi cultivar was pollinated by pollen from Beneh, Atlantica and Khinjuk (P. Khinjuk). The results showed that in the double pollination experiment, the nut, kernel and fruit set were affected more by the first pollen than by the second one. Pollen from the wild pistachio species reduced kernel weight, number of split nuts but increased percentage of the deformity and blank nuts in Owhadi. It was concluded that the effectiveness of the first pollen on fruit set, nut and kernel development was independent of the second one. The pollen of P. vera proved to be the best pollen source for pistachio cultivars.
H. Arabnezhad, M. Bahar, A. Taj Abadi Pour,
Volume 12, Issue 45 (10-2008)
Abstract
Microsatellite DNA markers isolated from wild species khinjuk (Pistacia khinjuk Stocks.) were used to evaluate the genetic diversity available in Iranian pistachio cultivars. Out of the 27 SSR primers tested initially, 25 could amplify the DNA in different pistachio cultivars, of which 19 primer pairs produced clear bands. Based on the amplification profiles of the genotypes by the remaining primer pairs, eight primers produced a monomorphic product and other 11 microsatellites markers were found polymorphic among the genotypes. The number of putative alleles amplified by each polymorphic SSR locus ranged from two to eleven alleles with a total of 48 alleles. An average of alleles and observed heterozygosity per locus was 3.69 and 0.69 respectively, showing that these microsatellites are highly informative for pistachio fingerprinting. The UPGMA cluster plots based on nei index placed the 20 commercial pistachio cultivars into a major group containing three distinguished subgroups however, genotypes, namely, Ghazvini zudras and Sarakhs (wild P. vera), were clearly situated into two distinct clusters, distant from the domesticated genotypes studied here. Both Ghazvini zudras and Sarakhs are known as small-fruited genotypes which are grown in restricted regions. Therefore, the distinctness of these genotypes can be attributed to their geographical isolation and morphological characteristics. It seems that Ghazvini zudras probably originated from Sarakhs variety which posses an important role in development of pistachio cultivars. The present study revealed that the khinjuk pistachio microsatellites are well distributed in the genome of P.vera , and are informative for estimating the extent of genetic diversity and characterization of pistachio cultivars.
