M. Heidari, E. Tafazoli,
Volume 9, Issue 2 (7-2005)
Abstract
One of the main criteria for salt tolerance is cell membrane stability under stress. Reactive oxygen species (ROS), activity of lipoxygenase (Lox) and lipid peroxidation are considered to be destructive to cell membrane under salt stress. In this study, the effects of 0, 75 and 150 mM NaCI, over a period of 14 days on the activity of lipoxygenease, the amount of hydrogen peroxide (H2O2) and malon dialdehyde (MDA. as a lipid peroxidation) in the leaves of Pistacia vera L. (cv. 'Qazvini' and wild 'Sarakhs' pistachio) and 'Mastic' (P. mutica F. & M.) were studied. The results indicated that by increasing salinity Lox activity increased in the leaves of all the three rootstocks. This activity reached a climax on the 7th day and then decreased on the 14th day. Among the three species, Mastic reached the highest amount of the Lox activity on the 14th day with the lowest amount of Lox reduction. The amounts of H2O2 in the leaves of all the three rootstocks increased, on the 14th day the highest amount of hydrogen peroxide was found in 'Mastic' and 'Sarakhs' after treating them with 150 mM NaCl. The amounts of MDA were also reached the highest level in all three rootstocks on the 7th and 14th days. The results also indicated the possibility of the use of lipid peroxidation index and Lox activity for selecting salt tolerant Pistacia rootstocks. More studies are needed for understanding the biochemical changes and enzyme activities in Pistacia rootstocks under salt stress.
H.r. Rahmani, A.a. Gheisari, R. Taheri, A. Khodami, M. Toghiani,
Volume 12, Issue 43 (4-2008)
Abstract
This experiment was conducted to evaluate the effects of 7 experimental diets (2 diets containing 100 and 200 mg vitamin E (VE)/kg and 5 diets containing 0, 0.5, 1, 1.5 and 2% green tea powder (GTP) ) on performance and breast and thigh meat oxidative stability of broiler chicks during different storage times. A total of 364 male broiler chicks were divided to 28 groups of 13 chicks each. Then every 4 groups were randomly allocated to one of experimental diets. During the experimental period (7-42 days of age) feed intake, weight gain and feed conversion of the experimental groups were measured. On day 42, 2 birds from each replicate (8 chicks per treatment) were weighed, slaughtered and their muscles of breast and thigh were minced and stored at 4ºC. Meat oxidative stability of the samples was determined on days 0, 6 and 9 of storage time using thiobarbituric acid test (TBA test). The results showed that addition of the VE and GTP to diets tended to significantly (P<0.05) reduce live body weight, weight gain, feed intake and abdominal fat accumulation and increase feed conversion ratio and pancreas size compared to control group. Increasing storage time from 0 to 6 and 9 d significantly (P< 0.05) increased TBA number. Lipid peroxidation and TBA values in thigh muscle were significantly (P<0.05) higher than breast muscle (1.46 vs. 0.359 mg Malonaldehyde/kg meat). Different dietary levels of GTP in diets did not have any significant effect on prevention of meat lipid peroxidation. In addition, the values of TBA in meat of the groups fed diets containing high levels of GTP significantly (P<0.05) increased in comparison to control group and those fed VE supplemented diets.
