Showing 4 results for Medicago
A. Karimi, E. Rowghani, M. J. Zamiri, M. Zahedifar,
Volume 8, Issue 1 (4-2004)
Abstract
An experiment was carried out to determine the effect of substituting alfalfa hay (Medicago sativa) with Gundelia tournefortii hay (a range forage in Fars Province) in the ration of fattening lambs. Alfalfa hay was substituted with Gundelia tournefortii harvested at the final stages of flowering at various ratios (0, 25, 50, 75, and 100% dry matter basis) in the ration of fattening lambs. The rations (iso-nitrogenous and iso-energetic) were pelleted and each ration was fed for 75 days to 12 Shirazi Grey ram lambs with an average age of 136 days. The lambs were slaughtered and their feedlot performance and carcass characteristics were measured. Substituting alfalfa hay with Gundelia tournefortii hay did not significantly affect the mean daily weight gain, feed consumption, feed conversion ratio, dressing percentage, internal fat weight, carcass meat content, or meat DM and CP contents (p>0.05). However, backfat depth was significantly different among various rations (p<0.01). At present costs, the inclusion of Gundelia tournefortii in the ration of fattening lambs seemed to be cost-effective the cost of one kg DM of ration was 1266, 1155, 1050, 924, and 787 Rials for rations 1 to 5, respectively.
M. Akhondi, A. Safarnejad, M. Lahouti,
Volume 10, Issue 1 (4-2006)
Abstract
Drought stress is one of the most important environmental stresses in reduce of growth and plants production. Determination of resistance mechanisms to environmental stress in plant improvement is very important. In order to , experiment with osmotic potentials of PEG (zero (control), -4, -8 & -12 bar) and alfalfa genotypes was done. The selected alfalfa genotypes namely, Yazdi(tolerant), Nikshahri(moderate) and Ranger(sensitive) were grown in hydroponic culture. After 4 weeks, they were harvested in order to determination of proline accumulation and Ca+2, K+ and Na+ concentration. The data showed with increasing of drought stress, proline accumulation were increased, but its rate was different among genotypes and organelles. Concentrations of K+, Na+ and Ca2+ increased with increasing of osmotic stress and there was significant different between genotypes. The K+/Na+ ratio in the shoots and roots of plant was decreased, when drought stress increased. Morphological and biochemical data showed Yazdi genotype was more tolerant to drought stress in compare with studied genotypes.
M. Falahati-Anbaran, A. A. Habashi, M. Esfahany, S. A. Mohammadi, B. Ghareyazie,
Volume 10, Issue 3 (10-2006)
Abstract
Annual medics are used for hey production, soil protection, biological fixation of N2 and green manure. In the present study, the inter and intra specific genetic diversity and relatedness of 4 diploid and two tetraploid (M. rugosa and M. scutellata) annual medics were evaluated using microsatellite markers. PCR analysis was performed on genomic DNA from individual plant and PCR products were detected using standard polyacrylamide sequencing gel. Totally twenty five polymorphic alleles were observed in the studied species. Average intra-specific genetic diversity ranged from zero (0.0) in both M. rugosa and M. scutellata to 0.114 in M. minima species, and the level of genetic diversity was similar in both M. orbicularis and M. truncatula species. Analysis of molecular variance (AMOVA) was used to partition the overall genetic diversity into within and among species, and between diploids and tetraploids. The results revealed significant (P<0.05) inter and intra-specific genetic variation. Pairwise comparisons based on Fst indicated significant differences among all of the species. Clustering analysis using UPGMA algorithm based on coancestary coefficient revealed a clear genetic relationship among species. The hypothesis on a common origin of two tetraploid species was supported by UPGMA clustering and phylogenetic analysis. The high level of Genetic diversity in spiny pod species respect to spineless pod species suggested the high importance of species with spiny pods in annual medics evolution. The findings support the usefulness of microsatellite markers for assessing inter and intra specific genetic diversity, differentiation and genetic relationships.
M Talebi, M Bahar, Gh Saeedi, A Mohamadi,
Volume 13, Issue 47 (4-2009)
Abstract
To characterize the geographical distribution of medicago-nodulating rhizobia in western regions of Iran, 950 Sinorhizobium isolates were trapped from a combination of two local alfalfa populations (Hamedani, Nikshahri) together with a foreign cultivar ( Kodi) and soil samples from eight sites across Kurdestan, Kermanshah, Eastern Azarbayjan and Lorestan provinces. Also, a total of 45 isolates were obtained from nodules of naturally grown Melilotus officinalis (14 isolates) and Trigonella foenum-graecum (31 isolates) plants in Isfahan. On the basis of PCR partial amplification of the plasmid born nod box gene and chromosomal mucR gene of the isolates,16S ribosomal DNA PCR-restriction fragment length polymorphism analysis, and the nucleotide sequence, three isolates from alfalfa, seven isolates from M. officinalis and 13 isolates from T. foenum-graecum were proved to be Sinorhizobium medicae. The remaining isolates (943 from alfalfa, seven from M. officinalis and 18 from T. foenum-graecum) were identified as S. melilloti. Both species, S. meliloti and S. medicae, were recovered from nodules of all the hosts although S. meloti was clearly more dominant in nodulating different populations of alfalfa. Taken together, these results indicated that the abundance of S. meliloti is independent of the site of isolation and have a wide geographical distribution. In this study, the banding pattern resulting from PCR amplification of 16S rRNA gene, followed by digestion with Rsa I, clearly differentiated S. meliloti and S. medica strains, showing that PCR-RFLP is an appropriate method to discriminate medicago-nodulating rhizobian with relative rapidity.
