Bahram Sharifnabi, Asghar Nekoei,
Volume 1, Issue 2 (10-1997)
Abstract
In order to study fungi associated with seeds of sainfoin, several seed samples of sainfoin were collected from Isfahan, Ardabil, Zanjan and eastern Azerbaijan provinces, Iran. Seed lots, only half of which were surface sterilized, were allowed to grow on PDA, SMA, MA, sterilized wet paper and drenched sands. The dishes containing seeds were incubated at 25°C with alternate cycles of 12-hour fluorescent light. After seven days, fungi growing from the seeds were transferred to the selective media and subjected to morphological studies. Single spore or hyphal tips from these transfers were the basis for all identification procedures. The most prevalent fungi associated with sainfoin seed were Alternaria, Aspergillus, Penicillium and Rhizopus, although Ulocladium, Cladosporium, Fusarium, Mucor, Nigrospora, Stemphylium, Trichothecium and Botrytis were also isolated in low incidence from these seed lots. The isolates of Uromyces and Oidiopsis were also obtained when the seed samples were examined by washing method.
Mohammad Hajian, Vahid Jahanbakhsh,
Volume 2, Issue 1 (4-1998)
Abstract
Seeds of Haloxylon (H. pericae, H. aphyllum, and H. ammmodendron) were collected from Sabzevar and Gonabad regions. The seeds were kept in the refrigerator for isolation of the fungi after determining the germination rate and moisture content. The recommended methods by International Seed Testing Association (I.S.T.A.) for isolation of seedborne fungi were followed throughout this study. The fungi were isolated by agar plate method, standard blotter technique, Hiltner test and seed analysis. In this study 15 genera and 19 species of fungi were identified. Among them Alternaria, Aspergillus, Camarosporium, Fusarium, and Penicillium were dominant among fungal genera isolates.
