Showing 16 results for Mycorrhiza
M. Hajian Shahri, M. Abbasi,
Volume 8, Issue 4 (1-2005)
Abstract
In order to investigate variations of spore population, root colonization and also to determine mycorrhizal symbiosis in the root and rhizosphere of Pistachio trees (Pistacia vera) in natural forests, two study stations in Kalat (Chachaeh) and Sarakhs (Shorlogh) regions were selected. Sampling from soil and root of the trees were taken from under the canopy and from a depth of 30 cm. On a monthly basis. The roots were stained and the colonization rate and the variations of spore population were measured. Some soil characteristics including pH, moisture, organic material percentage and available phosphorus were determined, The correlation coefficients between the measured factors were calculated. The results indicated that vesicular – arbuscular mycorrhiza (VAM) was the only symbiotic mycorriza of pistachio trees. Average amounts of root colonization were 13% and 11% in Chahchaheh and Shorlogh stations, respectively. Also, average numbers of spores per 1 gram of dry soil in the above stations were 12 and 10, respectively. The correlation between the variation of spore population and colonization levels was positive but the correlation between spore population and soil moisture, organic material, available phosphorus and pH was negative.
M. Zarei, N. Saleh-Rastin, Gh. Savaghebi,
Volume 15, Issue 55 (4-2011)
Abstract
A greenhouse experiment was conducted in factorial experiment arranged as a completely randomized design (CRD) to evaluate the role of tree indigenous arbuscular mycorrhizal fungi (AMF) species originated from a polluted soil in phytoremediation of zinc polluted soils using maize as a host plant. The experiment consisted of plants inoculated with AMF (G1(Glomus intraradices), G2(Glomus mosseae) and G3(Glomus versiforme)) and G0 as non-inoculated plants and 5 levels of zinc (0, 10, 50, 100 and 500 mg kg -1) in non-sterilized sandy loam soil with three replications. According to the results of greenhouse experiment, the zinc and phosphorus uptake and also the biological yield of maize plants were significantly increased by inoculation with AMF in comparison with non-inoculated plants and also no zinc toxicity symptoms were observed. Uptake, translocation, and phytoextraction efficiency of plants inoculated with G. intraradices was more than the other treatments up to the level of 100 mg kg -1, but at the level of 50 mg kg -1 these amounts were highest in plants inoculated with G. mosseae. The efficicncy of three AMF in zinc uptake was highest at the low level of zinc. In general, under the high soil pollution (500 mg kg-1), G. mosseae was the most effective fungal species in Zn extraction and translocation while G. intraradices had the highest effectiveness for accumulation of Zn in the roots. The overall situation of G. versiforme was mostly between the two other fungal species.
F. Aghababaei, F. Raiesi,
Volume 15, Issue 56 (7-2011)
Abstract
The positive and beneficial effects of mycorrhizal symbiosis on the growth of various plants have already been documented. Most arable crops can mutually establish symbiosis with endo-mycorrhizal fungi, however, mycorrhizal associations with almond (prunus amygdalus), particularly in calcareous soils of arid and semi-arid regions, have not been studied. Thus, to realize the symbiotic association between mycorrhiza fungi and economically important native almond genotypes in Chaharmahal va Bakhtiary province, an experiment consisting of a randomized complete factorial design with three factors: almond genotypes (Mamaei, Rabee, Talkh, Sefid), soil phosphorus levels (0 and 150 kg P ha-1) and mycorrhizal treatments (almond inoculated with Glomus intraradices, Glomus mosseae and without inoculation) with three replications was conducted under greenhouse conditions by 4 months. Results showed that physiological traits including chlorophyll concentration, net photosynthetic rate and water use efficiency, were significantly increased for 20%, 300% and 300%, respectively in mycorrhizal almond plants, while transpiration rate was decreased
(8-10%). Although the genotype of almond and soil P were dependent on these factors, mycorrhiza species had the same effects. Increased available P in the soil enhanced the growth in all treatments and photosynthesis of nonmycorrhizal almonds.
V. Sarvi Moghanloo , M. Chorom, H. Motamedi , B. Alizadeh, Sh. Ostan,
Volume 15, Issue 56 (7-2011)
Abstract
Soil enzymes are the catalysts for important metabolic process functions including the decomposition of organic inputs and the detoxification of xenobiotics. The aim of this research was to determine the pattern of variation in the activities of dehydrogenase, urease, lipase and phosphatase enzymes, determining the number of hetrotrophic and degrading bacteria and measuring the soil respiration and yield plants during the bioremediation of oil contaminated soils. To this aim, the soil deliberately contaminated with crude oil at a 1 and 2 wt% rate and in four treatments including: plant multiflorum (T1), plant multiflorum with mycorrhiza inoculation (T2), plant multiflorum with oil degrading bacteria inoculation (T3), plant multiflorum with mycorrhiza and oil degrading bacteria inoculation (T4) was employed for bioremediation of oil contaminated soil. The above parameters were determined in five stages during bioremediation and ultimately for the yield of plants at the end of this period .The results showed that the activity of urease and hydrogenase anzymes were increased or decreased parallel to contaminant increase and decrease. In contrast, the activity of lipase anzyme was decreased with contaminant increase and increased with contaminant decrease. Therfore, it can be a good choice for monitoring of bioremediation of contaminated soils. The results showed that the number of degrading and hetrotrophic microorganisms were increased by increasing the amount of contamination and the number of degrading and heterotrophic bacteria were decreased parallel to contaminant decreasing especially in those samples treated with mycorrhiza inoculation. The plant yield and amount of degradation of oil compounds were highest in mycorrhiza plus degrading bacteria treatment.
H. Nadian,
Volume 15, Issue 57 (10-2011)
Abstract
Effect of drought stress and mycorrhizal symbiosis on growth and phosphorus (P) uptake by two sorghum cultivars different in root morphology was studied in a pot experiment. A factorial experiment with a randomized complete block design was performed. In this study, sorghum speed feed cultivar with high root proliferation and branching and sorghum KFS2 cultivar with low root proliferation and branching were colonized by Glomus intraradices. All plants were watered equally in the first three weeks to establish them. Drought stress was applied from the week four and the plants were watered when 40% (T1), 60% (T2) and 80% (T3) of the available water was consumed. Results showed that in both sorghum cultivars, plant dry weight decreased as drought stress was increased. However, dry weight of mycorrhizal plants was higher than that of non-mycorrhizal plants at all levels of drought stress. This increase for speed feed cultivar was greater than for KFS2 cultivar. Phosphorus concentration and total P uptake in shoot of both mycorrhizal plants were greater than those of non-mycorrhizal plants. P accumulation in both cultivars decreased by increasing drought stress due to the decline in plant biomass. However, P accumulation per unit length of colonized root increased by increasing drought stress. The greater percentage of root colonization and the increased total length of external hyphae per unit length of colonized root of KFS2 cultivar led to an increase in mycorrhizal growth response and improved P nutrition of KFS2 cultivar compared to those of speed feed cultivar.
M. Rahmanian, H. Khodaverdiloo, M. H. Rasouli Sadaghiani, Y. Rezaie. Danesh, M. Barin,
Volume 15, Issue 58 (3-2012)
Abstract
Arbuscular mycorrhizae (AM) and Plant Growth Promoting Rhizobacteria (PGPR) associations are integral and functioning parts of plant roots. These associations have a basic role in root uptake efficiency as well as improvement of plant growth in degraded environments including heavy metals contaminated soils. This study was conducted to evaluate the effects of heavy metal-resistant soil microbe's inoculation on bio-availability of Pb and Cd in soil, plant growth as well as metal uptake by Millet (Pennisetum glaucum), Couch grass (Triticum repens) and wild alfalfa (Medicago sativa). A soil sample was treated by different levels of Pb and Cd (soil 1). Native microbial inoculums were obtained from alfalfa rhizosphere soils adjacent to Pb and Cd mines in Zanjan region (soil 2), then added with weight ratio of 1:5 (w/w) to soil 1. Host plants including millet, couch grass, and alfalfa were grown in pots and kept in greenhouse conditions. At the end of growing period, shoot dry matter and Pb and Cd concentrations in plant and soil were measured. Results indicated that plants yield and Pb uptake were significantly higher in non-inoculated treatments (p ≤ 0.05). However, Cd uptake by plants was greater in inoculated treatments (p ≤ 0.05). Couch grass showed the most accumulation potential of Cd and Pb among the studied plants.
Y. Habibzadeh, M. R. Zardoshti, A. Pirzad, J. Jalilian,
Volume 16, Issue 60 (7-2012)
Abstract
To evaluate effect of different irrigation regimes and mycorrhizal fungi on the growth and yield of mungbean NM92 [Vigna radiata (L.) Wilczk], a field experiment was conducted in split plot arrangements using randomized complete block design (Irrigation after 50, 100, 150 and 200 mm evaporation from pan class A as main plots and mycorrhiza species, Glomus mosseae, G. intraradices and a non-inoculated treatment as sub-plots) with three replications at the Research field of Urmia university in 2009. Results showed that irrigation after 50mm evaporation from pan class A, and plant inoculated with G. intraradices produced the highest grain yield (1678.5 kg/ha and 1537.6 kg/ha, respectively), total dry weight, leaf dry weight, leaf area index, crop growth rate, relative growth rate and net assimilation rate. In Contrast, irrigation after 200 mm evaporation from class A pan and non-inoculated treatment produced the lowest grain yeild (1159.2 and 1301.9 kg/ha, respectively). Reducing the irrigation distance led to an increase in total dry weight, leaf dry weight, leaf area index, crop growth rate, relative growth rate and net assimilation rate. Despite lower grain yield in water deficit condition, AM fungi inoculation significantly reduced the effect of stress on grain yield. All inall, both mycorrhizae species significantly (P 0.05) increased the grain yield of mungbean under well-watered and water deficit conditions
M. Ghorchiani, Gh. Akbari, H. A. Alikhani, M. Zarei, I. Allahdadi,
Volume 17, Issue 63 (6-2013)
Abstract
In order to evaluate the effect of arbuscular mycorrhizal (AM) fungi and Pseudomonas fluorescens bacteria on phosphorus fertilizer use efficiency, mycorrhizal dependence and grain yield and dry matter yield of maize under water deficit conditions, a field experiment was conducted as split-split plot arrangement based on randomized complete block design with three replications. The treatments in this experiment were included as follows: irrigation (normal irrigation and water deficit stress based on evaporation from class A pan evaporation) combined different seed inoculations with AM and Pseudomonas fluorescens and treatment of chemical phosphate fertilizer (non- consumption of phosphate fertilizer, consumption of 50 percent of triple superphosphate fertilizer needed, and consumption of rock phosphate, based on the quantity of consumed phosphorus of triple superphosphate source). The results showed that effects of irrigation, seed inoculation with AM and Pseudomonas fluorescens biological phosphate fertilizer and chemical phosphate fertilizer were significant on yield and yield components, dry matter yield, relative agronomic efficiency and root colonization. Mild stress significantly reduced grain yield, relative agronomic efficiency and fertilizer agronomic efficiency compared to optimum irrigation. Results showed that grain and dry matter yield are highly correlated with root colonization under mild stress condition. Maximum mycorrhizal dependence was obtained in mild stress condition. Maximum relative agronomic efficiency, fertilizer agronomic efficiency, yield and yield components was related to seed inoculation with AM and Pseudomonas fluorescens. The use of triple superphosphate fertilizer were increased grain yield in comparison with rock phosphate along with inoculation of phosphate solubilizing microorganisms. However, maximum root colonization and mycorrhizal dependence was achieved in non- consumption treatment of phosphate fertilizer.
A. Ahmadnejad, J. Abedi Koupai, F. Mousavi,
Volume 17, Issue 66 (2-2014)
Abstract
Sesame (Sesamum indicum L.) is one of the most important oilseed crops in the world. Drought stress is one of the environmental factors limiting sesame production. The effects of water deficiency can be reduced by inoculation of plant roots with mycorrhiza fungi. In this experiment, the objective was to determine the effects of different levels of water application (60, 80 and 100% of soil moisture depletion) using surface drip irrigation method and inoculation of plants with mycorrhiza on WUEag of sesame. The experiment was arranged using a factorial design based on randomized complete block design with three replications. The field experiment was conducted at the Agricultural Research Farm of Isfahan University of Technology in 2011. Results showed that the effect of irrigation regime on WUEag of seed yield was not significant. However, the effect of mycorrhiza on WUEag of seed yield was significant. The highest WUEag (0.74 kg/m3) was related to irrigation regime of 80% and mycorrhiza. The effect of irrigation regime and mycorrhiza on WUEag of oil yield were significant. The highest WUEag (0.43 kg/m3) was related to irrigation regime of 100% and mycorrhiza inoculation.
V. Dorostkar, M. Afyuni , A. H. Khoshgoftarmanesh, M. R. Mosaddeghi , F. Rejali,
Volume 19, Issue 73 (11-2015)
Abstract
Arbuscular mycorrhizal fungi (AMF) are widespread endosymbionts in terrestrial ecosystems and their exudates have important effects on soil properties. A greenhouse experiment was conducted with six AMF treatments including four exotic species inoculums (Funneliformis mosseae ,Claroideoglomus claroideum and Rhizophagus irregularis and a mixed isolate of three species), one mixed native AMF species treatment and a sterilized soil (control) with four salinity levels (1, 5, 10 and 15 dS m-1). AMF increased the soil (EEG) and total (TG) extractable glomalin, and also the hot water (HWC) and diluted acid (DAC) extractable carbohydrates compared to control treatment in all salinity levels. The native AMF species had the greatest effects on EEG, TG, HWC and DAC at 10 and 15 dS m-1. Soil EEG and TG concentrations were higher in the mixed exotic AMF treatment than in each AMF species. The greatest glomalin concentration was related to F. mosseae at 1, 5 and 15 dS m-1 but at 10 dS m-1 the greatest glomalin concentration was related to C. claroideum. The greatest carbohydrate concentration was related to F. mosseae at 1 and 5 dS m-1 but at 15 dS m-1 significant differences were observed among the three AMF species. Our results showed that there is an interaction between salinity and different AMF species, and a combination of them determines the function of AMF.
S. Moradi Behbahani, M. Moradi, R. Basiri, J. Mirzaei,
Volume 20, Issue 78 (1-2017)
Abstract
Salt cedar is widely spread out in most part of the country but there is lack of information about its symbiosis with arbuscular mycorrhizal fungi. Then, the main objective of this study was to evaluate the symbiosis of AMF with salt cedar and its affectability by distance from river and soil physiochemical properties. For this purpose, riparian Maroon forest width was divided to three locations including riverside area, intermediate area and the area far from river with 200-hundred-meter interval. In each site 10 salt cedars were randomly selected and soil plus hair root samples were gathered from the salt cedar rhizosphere. Our result indicated that root colonization and spore density in the intermediate distance had the lowest and highest values, respectively. These values were significantly different compared to the other two sites. The average root colonization percent in the riverside area, intermediate area and the area far from river sites were 82.37, 73.77 and 80.17, respectively. While the average spore density in the riverside area, intermediate area and the area far from river were 189, 245.5 and 188.8 in five gram soils, respectively. Root colonization had significant positive correlation with soil potassium while spore density had significant correlation with studied soil physiochemical properties. Also, soil nitrogen, organic carbon, potassium and clay showed 52.6, 51.19, 50 and 23.4% decreasing trend from the riverside area to the area far from river. Regarding this research results, salt cedar showed high level of symbiosis with arbuscular mycorrhizal fungi but this symbiosis could be affected by distance from river in riparian forest.
M. H. Rasouli0-Sadaghiani, H. Khodaverdiloo, M. Barin, S. Kazemalilou,
Volume 22, Issue 1 (6-2018)
Abstract
The use of plants and soil microorganisms is a promising technique for the phytoremediation of heavy metal-contaminated soils. This study was carried out in order to evaluate the soil microbial potential with four Cd concentration levels (0, 10, 30 and 100 mg kg-1); the study also addressed the inoculation of arbuscular mycorrhizal fungi (AMF) species (a mixture of Glomus species including G. intraradices, G. mosseae and G. fasciculatum) as well as plant growth promoting rhizobacteria (PGPR) (a mixture of Pseudomonas species including P. putida, P. fluorescens, and P. aeruginosa) with the Centaurea cyanus plant. The soil sample was spiked uniformly with Cd nitrate salt to create different Cd concentrations. The contaminated soils were then sterilized and subsequently inoculated with AMF and PGPR. The results indicated that with increasing the soil Cd concentration, colonization percent, abundance of rhizobateria, shoot biomass, and shoot relative biomass were significantly decreased, while the proline content and the shoot Cd concentration were significantly increased (P≤0.05). The mean of Cd extracted in AMF and PGPR treatments was 1.8 and 2.8 and the translocation factor was 1.2 and 1.5 times higher, as compared to the corresponding control treatments, respectively. It could be concluded that microbial inoculation, in addition to improving plant growth, plays an important role in the Cd phytoremediation efficiency by plant.
A. Hemmati, H. Asadi Rahmani,
Volume 22, Issue 4 (3-2019)
Abstract
In order to study the effects of rhizobium bacteria and arbuscular mycorrhizal fungi on water use efficiency and the grain yield of bean, under drought stress conditions, two-year year field experiments were conducted during 2015 and 2016 growing seasons. The experimental design was a split plot arranged in an RCBD with 4 replications. Three irrigations including S1= 95-100% AW (Normal irrigation), S2= 75-80% AW(Moderate drought stress) and
S3= 55-60% AW (severe drought stress) were assigned to the main plots and six bio fertilizer treatments including T1=177 rhizobium bacteria strain, T2=160 rhizobium bacteria strain used for seed inoculation, T3= mycorrhizal arboscular fungi used for soil inoculation, T4= T1+T2, T5= T1+T2+T3 and T6= control (no seed and soil inoculation) were randomized to the subplots. Based on the combined analysis of variances for two years, there were significant differences (p˂0.05) in the grain yield, yield components and water use efficiency between the irrigation stress and bio fertilizer treatments. The highest grain yield (2371 kg ha-1) and water use efficiency (522 g m-3) were obtained in the S2T2 treatment. In this treatment, 160 and 177 rhizobium bacteria (T2) in moderate drought stress (S2) were used. These results suggested that inoculation with the rhizobium of seed bean in arid and semi-arid areas could improve yield, water use efficiency and resistance to drought stress by increasing the growth in the root and shoot of the plant.
S. Abdi, A. Pirzad,
Volume 23, Issue 1 (6-2019)
Abstract
Water stress is one of the most important factors limiting the growth and production of crops in arid and semi-arid regions. To evaluate the effect of mycorrhizal fungi species on the growth and yield (quantity and quality) of Onobrychis sativa under water deficit condition, a greenhouse factorial experiment based on completely randomized design (CRD) with three replications was conducted in 2014. Treatments included five species of mycorrhizal fungi (Fanelormis mosseae, Rhizophagus intraradices, Claroideoglomus claroideum, Funneliformis caledonius, Glomus versiforme and non-mycorrhizal control) and two levels of irrigation (irrigation at 80% [well watering] and 50% [water deficit] field capacity [FC]). The highest grain yield (9.187 g/plant) was obtained from the stressed plants inoculated with Rh. intraradices with the same grain yield of F. mosseae inoculated plants (8.867 g/plant). With a significant reduction in the grain yield of stressed plants, mycorrhizal relationships even increased the yield more than the well-watered plants. Despite the decreases in the grain protein and phosphorous of water-deficit stressed mycorrhizal plants, the highest grain protein content was obtained from the plants inoculated with G. versiforme, and the highest grain phosphorus content was obtained from the plants inoculated with F. mosseae. Mycorrhizal symbiosis enhanced the yield and the quality of Sanfoin grain in water deficit stressed plants due to reducing root volume against the stimulating root elongation. In this way, the species G. versiforme exhibited the greatest positive effect.
H. Aalipour, A. Nikbakht, N. Etemadi, M. Soleimani, F. Rejali,
Volume 23, Issue 2 (9-2019)
Abstract
Trees decline is a complex physiological disease that results from the interactions between several factors, one of which is heavy metal stress that ultimately leads to the death of trees. This experiment, which was conducted during 2016-2017 at the campus facility of the Department of Horticulture at Isfahan University of Technology, was conducted to investigate the effects of inoculation with arbuscular mycorrhizal fungi (AMF) (Rhizophagus intraradices and Funneliformis mosseae inoculated, and the combination of both species) and plant growth promoting rhizobacteria (PGPR), Pseudomonas Flourescens, on the growth responses of Arizona cypress (Cupressus arizonica G) to different concentrations of cadmium (0, 5, 10, 15, 20); this was done as a factorial experiment based on a completely randomized design, with three replications. The interactions between AMF, PGPR, and cadmium on potassium and iron concentration, height, and dry weight of Arizona cypress seedlings were significant. By increasing the concentration of cadmium in most of the treatments, the colonization, phosphorus, potassium and iron concentrations, height and dry weight of the shoot Arizona cypress seedlings were decreased, while the percentage of electrolyte leakage and proline content were increased. The AMF-inoculated plants increased phosphorus, potassium and iron concentrations, Height, shoot dry weight, proline content and reduced electrolyte leakage percentage, as compared to non-mycorrhizal (control) plants. In plants inoculated with both microorganism (mycorrhizal fungi and Pseudomonas), there was a positive effect regarding the concentration of nutrients such as potassium and iron; there was also the improvement of growth characteristics such as height and dry weight of the seedlings, as well as the appearance and freshness of the plant. The results, therefore, showed that inoculation of Arizona cypress seedlings with the combination of mycorrhizal fungi and Pseudomonas fluorescens bacteria could have a positive effect on the growth and survival of this tree under Cadmium stress condition.
S. Ghasemi Pirbaloti, S. Soodaee Moshaee,
Volume 28, Issue 1 (5-2024)
Abstract
Since the long-term sustainability of garden ecosystems is dependent on maintaining the soil quality, knowing the condition of the soils and investigating the effects of the activities on the soil properties is very important and effective in ecosystem management. To investigate the soil quality index of almond (Prunus dulcis) orchards under different managed methods in ChaharMahal va Bakhtiari province, soil samples were collected from three points in each orchard and finally classified into 6 groups (Saman, Ben, Shahrekord, Kiar, Ardel, and Farsan). To determine the soil quality index, soil characteristics including pH, EC, total and water-soluble organic carbon, basal and substrate-derived respiration, rhizosphere microbial population, and available soil P and K were analyzed. The results showed that almond orchard management in different regions affected the soil characteristics and the processes evaluated in this study. The monitoring of soil properties showed that pH 7.05 - 8.48, EC 0.23 - 2.91 dS/m, microbial respiration 0.44 - 8.57 mg CO2.100 g-1.day-1, organic carbon 2.09 - 44.79 g/kg, available phosphorus 1.5 - 122.3 mg/kg, and available potassium were between 91.2 - 3038 mg/kg. Soil quality index components including chemical components, microbial activity, microbial population, and soil organic carbon were determined. The contribution of soil salinity to soil quality obtained using factorial analysis was the highest (31%), followed by microbial carbon mineralization coefficient (27%), rhizosphere microbial population (24%), and water-soluble organic carbon (18%). The soil quality index values for Saman, Ben, Shahrekord, Kiar, Ardal, and Farsan almond orchards were 0.46, 0.40, 0.51, 0.67, 0.54, and 0.37, respectively. These values showed that the evaluated soils are suitable for almond production in Shahrekord, Kiar, and Ardal, and for Saman, Ben, and Farsan, there is a need for serious management measures to improve soil quality and increase the sustainability of these agricultural ecosystems.
