Showing 3 results for Nodulation
M. Sepehri, N. Saleh Rastin, H. Asadi Rahmani, H. Alikhani,
Volume 10, Issue 1 (4-2006)
Abstract
Heavy metals have deleterious the effects on nodulation and N2 fixation of Rhizobium- Legume symbiosis, due to their inhibitory effects on the growth and activity of both symbionts. This research has been undertaken to evaluate the effect of Cd tolerance of native rhizobial strains on diminution of the Cd detrimental effects on Sinorhizobium meliloti-alfalfa symbiosis. For this purpose, a greenhouse experiment was conducted based on Randomized Complete Block Design. The treatments in this experiment included: plants inoculation with 6 bacterial strains (sensitive, partially tolerant and tolerant to Cd), 5 levels of Cadmium (0, 2, 5, 10, 20 mg/kg soil) and non-inoculated control. In different levels of Cd, the effects of bacterial inoculation on root nodule number and total amount of nitrogen in plant shoot were compared. The results indicated that soil pollution by Cd even at 2 mg/kg had significant effect on symbiotic properties of rhizobial strains, and according to Cd tolerance of various strains, the mentioned effect was different. Decreasing effect of Cd concentration on root nodules and nitrogen concentration in plants that were inoculated with sensitive strains in comparison with plants inoculated with tolerant strains was 68.31% and 40.8%, respectively. In this research, R95m was introduced as the best strain because of its ability for nodulation and nitrogen fixation.
L. Rasipour, N. Aliasgharzadeh,
Volume 11, Issue 40 (7-2007)
Abstract
Certain microorganisms in soil have phosphate solubilizing ability. Phosphorus has an important role in plant nutrition and N2 fixation in legumes. The interactive effect of three phosphate solubilizing bacteria (PSB) and Bradyrhizobium japonicum on yield and N, P, K uptake and nodulation of soybean root (Glycin max L. CV. Harcor) was studied under greenhouse conditions. In greenhouse experiment treatments consisted of a factorial combination of four levels of PSB (without PSB M0 ,Pseudomonas putida M1 , Aeromonas hydrophila M2 , Pseudomonas fluorescens M3) and two levels of B.japonicum (without bacterium B0 and with bacterium B1) and three levels of P(P0 = 0 , P1=29, P2 = 58 mg triple superphosphate/Kg soil) in a randomized complete block design with four replications. At harvest, shoot dry weight, seed weight, nodule number, dry and fresh weight of nodules and concentrations of N, P, K in shoot dry matter were measured. PSB significantly increased shoot dry weight, N, P, K concentrations in shoot, fresh and dry weight and number of root nodules. B.japonicum had positive significant effect on these parameters as well as on seed weight. Dual inoculation of plants with PSB and B.japonicum had significant effect on shoot dry weight, P and N concentrations in shoot. Increasing phosphorus levels significantly increased plant dry weight, shoot P concentration and seed weight. Highest P concentration in shoot was obtained at P2 level but in plants inoculated with P.putida, dry weight at P1 level was not significantly different from P2 level.
M. Amini Dehaghi, S.a.m. Modarres Sanavy, F. Fattahi Neisiani,
Volume 12, Issue 45 (10-2008)
Abstract
In order to study the effect of planting dates and genistein on nitrogen content and nodulation of three annual Medicago species (Medicago polymorpha cv. Santiago M. rigidula cv. Ragidula, and M. radiata cv. Radiata), an experimental study was conducted during 2004-2005 on the Research Farm of Faculty of Agriculture, Tarbiat Modares University, Tehran (35°43′N and 51°8′E). The factors were arranged as split-split plot in a randomized complete block design with four replications. Planting dates (February 20, March 1 and 11) were randomized to main plots and three annual medics were located in sub-plots, and genistein (0 and 20 μM) was randomized to sub-sub-plot units. Plant nitrogen contnt, nodulation and other traits were significantly different in species and M. polymorpha was better than other species in view of dry nodule weight, nodule number, nodule number in each cluster, nodule cluster number and nodule diameter. Medicago rigidula had more resistance to cold than other varieties, and its forage yield and nitrogen percentage were better than M. polymorpha. Therefore, M. rigidula may be better suited for cold zones. Twenty μmol genistein had remarkable effect on nodulation and nitrogen percentage of annual medics in comparison with control. The result showed that genistein modified negative effect of low temperature environment on nodulation and nitrogen percentage of annual medics. Nodulation and nitrogen percentage increased in all varieties at the first planting date. This finding emphasizes that genistein has a considerable effect on cold resistance establishment in varieties for improving nodulation and increasing plant nitrogen percentage in farm condition.
