Showing 3 results for Protein.
M. Toghiani, A. Samie, A. Gheisari,
Volume 7, Issue 2 (7-2003)
Abstract
This experiment was conducted to investigate the effects of intensity of restricted early feed and increased nutrient density after restriction period on performance and compensatory growth in broilers. Two hundred and eighty-eight broiler (Lohman) chicks in a completely randomized design in a 2*3 factorial arrangement of treatments were employed. Birds were fully fed from 7 to 13d or 100% of adlibitum intake on a daily basis. The diets were diluted at 25% and 50% levels with rice hulls. After the restriction period, birds were fed with a diet, formulated to meet all their nutrient needs, according to the NRC (1994) or 10% of protein, methionine and lysine above NRC in the realimentation diet from 13 to 21d. Body weight, feed intake, daily gain and feed conversion were determined weekly and selected carcass characteristics (liver, intestine and abdominal fat pad) were measured at 49 days of age. Feed restriction significantly (P<0.01) decreased body weight gain and feed conversion during the restriction period. Birds were able to fully recover body weight depression up to 49d. The intensity of restriction had no effect on body weight, feed intake, daily gain and feed efficiency at 49d (p>0.05). Increased nutrient density had no effect on growth rate, feed intake and feed efficiency for fully-fed birds and restricted birds at 49d. The results from this experiment showed that broiler chicks may be expected to gain weight similar to chicks with fully feeding even after restricted early feed if fed with NRC diets. The effect may be due to compensatory growth.
A. Mostafaie, M. Chalabi,
Volume 10, Issue 3 (10-2006)
Abstract
Proteolytic enzymes play important roles in food and drug industries. Actinidin, the most abundant protein of kiwifruit is a cystein protease (EC 3.4.22.14). In the present study, protein contents and levels of actinidin in kiwifruit cultivars were assayed and the enzyme was purified by a simple procedure. Actinidin was purified using two steps: (1)) precipitation by ammonium sulfate and (2) ion-exchange chromatography on diethylaminoethyl Sepharose. Molecular mass and proteolytic activity of the purified enzyme were determined by SDS-PAGE and casein digestion test, respectively. Protein content of the cultivars was compared by three different methods, namely, Bradford, UV and Lowry methods. The yield and degree of enzyme purity were 65 and 95 percents, respectively. Molecular mass of the purified enzymes were estimated 29 KDa in reducing SDS-PAGE. Results of the protein assays showed that protein and actinidin contents of kiwifruit were overestimated using UV or Lowry methods for assay. It was concluded that Bradford method is more accurate than UV or Lowry methods for measurement of protein level in kiwifruit.
K. Nazem, Y. Rozbehan, S.a. Shodjaosadati,
Volume 12, Issue 43 (4-2008)
Abstract
The nutritive values of lemon and orange pulps treated with Neurospora sitophila fungus were assessed by chemical composition, in vitro digestibility, in situ (dry matter and nitrogen) degradation methods. The obtained data from the untreated and treated pulps with fungi were compared using t-test. Mean values of the chemical analysis for crude protein, ash, organic mater, NDF and ADF for untreated lemon pulp were 6.3, 6.2, 93.8, 21.3 and 17.9 for treated lemon pulp 25.1, 10.6, 89.4, 12.7 and 6.8 for untreated orange pulp 6.8, 6.5, 94.5, 26.1 and 20.3 and for treated orange pulp 23.2, 8.1, 91.9, 18.5 and 15, respectively. In all cases, a significant difference (P<0.01) between untreated pulp and treated pulp of lemon and orange was observed. Digestibility coefficient of DM, OM and DOMD of untreated lemon pulp were 79.3, 80.5 and 75.5 treated lemon pulp were 91.4, 93.5 and 83.5 untreated orange pulp were 81.5, 82.8 and 78.2% and treated orange pulp were 91.2, 94.5 and 86.9%, respectively. The digestibility coefficient for the treated pulps (lemon and orange) was significantly (P<0.01) higher than those untreated. The percentages of DM degradability of pulps in nylon bag after 48 hours of incubation with the out-flow rate of 0.05 were as follows: 66.3 for untreated lemon 75.2 for treated lemon 68.7 for untreated orange and 75.5 for treated orange. The DM degradability for the treated pulps (lemon and orange) was significantly (P<0.01) higher than those untreated. The effective degradability of protein after 48 hours of incubation in nylon bag with the out-flow rate of 0.05 was as follows: 12.1 for untreated lemon 73.7 for treated lemon 14.8 for untreated orange and 77.8 for treated orange. The effective degradability of protein for the treated pulps (lemon and orange) was significantly (P<0.01) higher than those untreated. In conclusion, treating citrus pulps with Neurospora sitophila increased the concentration of CP, digestibility coefficients and protein degradability.
