Showing 2 results for Rapd Marker
C. Ghobadi, M. Khosh-Khui, B.e. Sayed-Tabatabaei,
Volume 12, Issue 45 (10-2008)
Abstract
Grapevine (Vitis vinifera L.) is a clonally propagated major fruit crop. In grapevine, identification of genotypes with amplographical features is often based on mature plant characteristics that may be affected by environmental conditions. This approach lacks objectivity and reliability. Recently, molecular markers have proved to be supplementary techniques to analyze genetic diversity and examine genetic relationships existing between cultivars in a range of horticultural crops. In this study, twenty genotypes from grapevine (V.vinifera species) grown in Isfahan province were characterized by RAPD technique to understand the extent of diversity and relatedness. Fifty random primers were used for the RAPD study. Of those, twenty four informative primers which generated reproducible polymorphic bands were used for grouping the genotypes. PCR products of the genotypes’genome revealed a total of 315 bands, out of which 282 were found to be polymorphic. Average number of 13 bands was obtained per primer and the amplification produced ranged in size from 300 bp to 3000 bp. The dendrogram constructed using UPGMA cluster analysis differentiated the genotypes into two major clusters, nineteen in one group and Madar-o-Bache genotype has been placed in a separate one, indicating its high genetic diversity compared to the rest of the genotypes. Intra-clustering within cluster A grouped the genotypes in four sub-clusters as expected from their genetic background. The results of the study revealed that the RAPD technique is a relevant technique to determine genetic diversity, genomic analysis and to examine genetic relationship in grapevines.
R Ravesh, B Shiran, A Alavi, J Zarvagis,
Volume 13, Issue 47 (4-2009)
Abstract
In this study, 45 Pleurotus isolates with a wide range of geographical origins (in five provinces of Iran: Isfahan, Chaharmahal Bakhtiary, Kohkiloye boyrahmad, Lorestan and Fars) were collected. A random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to assess the genetic diversity in ten populations of the Pleurotus eryngii complex. The use of ten primers allowed the production of 150 polymorphic bands. In addition, the evalution of the principal ecological and morphological characters provided further evidence for discriminating between populations. The results show a higher level of diversity of RAPD polymorphism in the populations. 28.67% of the RAPD diversity was among populations and 71.33% was within populations. In the RAPD patterns, Pleurotus strains growing on Ferula assa-foetida, Ferula ovina and Prangus ferulacea formed distinct groups. In this study, the resolution of the RAPD-PCR analysis was better than the morphological analysis.
