H Askarian, B Sharifnabi, M Olia, E Mahdikhani, A Akhavan,
Volume 13, Issue 47 (4-2009)
Abstract
Root knot nematodes (Meloidogyne spp.) cause yield loss in all countries, of which, M. javanica, is the most widespread species in Iran. In order to identify M. javanica, 100 infected root and soil samples of root knot nematode were collected from different regions of Kerman province. After purification of populations and identification of M. javanica based on morphological and morphometerical characters of females and second stage juveniles (J2), total DNA was extracted from eggs, J2 and female adults. Specific 670 and 1600 bp bands were amplified in all M. javanica populations using species-specific primer pairs including OPARjav / OPAFjav and Mjavf / Mjavr These specific bands could not be amplified in other species such as M. incognita and M. arenaria. It seems that, application of these species specific primers in comparison with morphological characters would be more applicable, leading to easier identification of M. javanica.
A. Abdolahi Arjenaki, A. A. Fadaei Tehrani,
Volume 19, Issue 71 (6-2015)
Abstract
Plant parasitic nematodes, especially root knot nematodes, cause damage to most of agricultural products, and many efforts have been done to control them. In recent years, application of industrial waste and wastewater sludge as organic fertilizers in agriculture has been increased. To investigate the effects of sewage sludge on root knot nematode pathogenicity in tomato, different weights of sewage sludge (0, 4, 8, 15 and 25% of sludge in the soil) in soil were added as a completely randomized design with six replications. Analysis variance and mean comparison of growth indices of plants showed significant effects of treatments. For example, means of stem length of plants increased in soil with more amount of sewage sludge. This relationship was also observed in other indices and nutrients elements. So using sewage increased nitrogen, phosphorous, calcium and magnesium in tomato shoots inoculated with nematodes while the change of potassium was very small. Application of sewage sludge decreased the number of galls, egg-masses and eggs in egg-mass of root knot nematode.
