JWSS - Journal of Water and Soil Science

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Land application of sewage sludge is potentially beneficial as an inexpensive nutrient source. However, problem with the use of sludge may exist from high soil concentrations and subsequent uptake of heavy metals by plant and entering of the metals into the human and animal food chains. A field study with lettuce (_{Lactuca sativa} L.) and spinach (Spinacia oleracea L.) was conducted to examine uptake of heavy metals from a sludge amended soil as affected by sludge rate and time of sludge application. Sludge rates were 0, 22.5, and 45 ton/ha. To determine the effect of time on heavy metal uptake, a year after the first plants were harvested, one third of each plot was planted without sludge application and to the rest of each plot sludge was added in the same rates as before. Total and EDTA-extractable Cu, Zn, Pb, and Cd concentrations in soil were determined. The metal concentrations in shoots and roots of the plants were determined separately. Total metal concentrations showed an increasing trend with addition of sewage sludge. Copper, Zn, and Pb EDTA-extractable concentrations in soil and concentrations of these metals in the plants increased significantly with sludge rate. Time of sludge application did not have any significant effect on EDTA-extractable and plant uptake of metals. Sewage sludge also increased the crop yields significantly.

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Due to high production of organic residues such as sewage sludge and compost, land application is probably the best way to prevent accumulated residues in the environment. However, the risk involved in the land application of organic residues should be evaluated prior to the assessment of their economic and fertilizer values. The objective of this study was to evaluate the effect of organic residues on soil chemical properties, yield and uptake of heavy metals and nutrients by corn (Zea mays L.). The study was a randomized complete block design with three replications with four treatments including cow manure, sewage sludge, municipal compost and inorganic fertilizer.

The organic amendments were applied to the plots at a rate of 50 t ha^-1 and inorganic fertilizer treatment consisted of 250 kg ha^-1 ammonium phosphate and 250 kg ha^-1 urea.

 Organic amendments significantly increased soil organic matter content, EDTA-extractable Fe, Zn, Cu and Pb and plant available P, K, and N. Uptake and concentration of nutrient in corn grain and shoots were significantly affected by treatments, whereas Cd and Pb concentrations were the same among the treatments. Cow manure and sewage sludge treatments had the highest and compost had the lowest corn silage and grain yields. Overall, the results indicated that cow manure and sewage sludge had a high fertilizer value and led to heavy metal concentrations in soil as well as corn tissues which were much lower than the reported standards.

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A field experiment was conducted to investigate the effects of sewage sludge and of time lapse after sludge application on soil physical properties. Four sewage sludge treatments (0, 25, 50, and 100 ton/ha) in a complete randomized block design with three replications were applied and mixed to a depth of 20 cm. Wheat was planted and soil physical properties were measured 23, 85, 148, and 221 days after sewage sludge application. Sewage sludge application significantly increased MWD, hydraulic conductivity, final infiltration rate, moisture percentage at 1/3 and 15 bars, and plant available soil moisture, while it significantly decreased soil bulk density. In general, the best results obtained with the 100 ton/ha sewage sludge treatment. Time lapse after sewage sludge application caused soil physical properties to approach the values of the control. However, even 221 days after sludge application, the 50 and 100 ton/ha treatments had significantly different values compared with the control treatment. The results in this research show that sewage sludge can help to improve soil physical conditions and this effect persists over long periods. This effect is specially important with plant available soil moisture and infiltration.

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Excessive application of sewage sludge leads to the accumulation of potentially toxic elements in soils. The objective of this greenhouse study was to examine the DTPA–extractability of Fe, Zn, Cu, Mn, Pb, Cd, Ni, and Co in relation to soil pH and to investigate the concentrations of these metals in corn (Zea mayz). The study was conducted using a factorial experiment in a completely randomized design with three replications. Sewage sludge was applied at 0, 50, 100, and 200 t/ha on four soils [Langaroud (pH= 4.8), Lahijan (pH= 5.7), Rasht (pH= 6.8), and Isfahan (pH= 7.9)]. Application of sewage sludge significantly increased DTPA-extractable Fe, Zn, Cu, Pb, Cd, and Ni in all soils. The increasing effect was in accordance with sludge level. Langaroud soil had the highest and Isfahan soil the lowest levels of DTPA-extractable metals. Application of sewage sludge increased plant growth and metals in the foliage of corn plants. The metal concentration in corn tissues also increased with decreasing soil pH. Overall, the application of sewage sludge as an organic material, particularly in acid soils, may increase availability of heavy metals, which may, in turn, result in soil pollution. Therefore, addition of sewage sludge to soils should be managed on the basis of the changes in soil heavy metal concentration.

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Sewage sludge and effluents, as cheap sources of irrigation water and fertilizer, can supply plants with water and nutrients however, contamination of these sources with heavy metals and the possibility of human food chain contamination using these sources should be considered. In this research, the effects of industrial sewage sludge and effluents on concentration of some nurtients, heavy metals and sodium and dry matter yield of wheat (Triticum aestivum), barley (Hordeum vulgare) and corn (Zea mays) were investigated. The experiment was carried out in a greenhouse using a complete randomized design with four replication. The treatments comprised well water, well water + sewage sludge(50 tons/ha), and three industrial effluents from Iran Polyacryl factory including the cooling tower, the over flow and the factory outlet effluents. Chemical analysis showed the following results: The concentration of the elements in the sludge and the effluents were below the critical contaminating levels. The application of the treatments did not supply enough nitrogen for corn the cooling tower effluent could not supply enough nitrogen for wheat and barley all the treatments supplied enough P for wheat. None of the treatments could supply enough P for corn. The cooling tower, over flow and the factory outlet effluents could not supply enough P for barley, the micronutrient and heavy metal concentrations in the plant tissues using the effluents and the sewage sludge were higher than those for well water the dry matter yield of plants’roots and shoots was highest using well water + sludge and in comparison with the well water, effluents could increase the shoot dry matter yield.

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Application of sewage sludge on the farmland as a source of crop nutrient had recently received considerable attention. Some management practices may be required to control the accumulation of toxic elements including Pb, Ni and Cd. Sequential extraction gives useful information on plant bioavailability of the elements. The objective of this study was to investigate the cumulative and residual effects of sewage sludge application on the chemical forms and mobility factor of Pb, Ni and Cd. Zero, 25, 50 and 100 Mg ha-1 of sewage sludge were applied for 1, 2 and 3 consecutive years in a split plot design, with three replications. Soil samples were taken from 0-20 cm at the end of the third year of application. Different chemical forms of Pb, Ni and Cd were measured. Results revealed that the soluble form (SOL) of Ni and Cd increased whereas Pb soluble form decreased with increasing levels and years of application. Exchangeable (EXC), carbonate (CAR) and organic (ORG) forms of the three elements increased as levels and years of application increased. Occluded (OCC) form decreased for Pb, Ni and increased for Cd. Residual form (RES) of Pb increased while that of Ni and Cd decreased. A gereral increase was observed for available (DTPA-extractable) concentration of Pb, Ni and Cd. Relative distributions of different chemical forms were in the following order: Pb: OCC > RES> ORG> CAR> EXC> SOL, Ni: RES> ORG> OCC> CAR>EXC> SOL and Cd: OCC> CAR> RES> ORG> EXC> SOL. The comparison of different forms of the metals showed the following orders: soluble Ni>Pb>Cd, exchangeable, carbonate and occluded Pb>Ni> Cd, organic and residual Ni> Pb>cd. Increasing the available (DTPA-extractable) concentration of the elements in such a calcareous soil showed that consecutive application of sewage sludge may increase the available (DTPA-extractable) concentration beyond critical levels. A significant corretation was observed between organic form and available (DTPA-extractable) concentration of the elements.

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Sewage sludge application on farmland as fertilizer is commonly practiced in many countries. Sewage sludge is rich in macro- and micro- nutrients. However, high concentration of heavy metals in sludge may cause pollution of soil, groundwater and human food chain because of uptake of toxic metals by crops. The objective of this study was to determine residual and cumulative effects of sewage sludge on concentration of Pb and Cd in soil and wheat. Different levels of 0, 25, 50 and 100 Mg ha^-1 of sewage sludge were applied to the soil for four years. To study the cumulative and residual effects of the sewage sludge, applications were repeated on three fourth of each plot in the second year, on one half of plots in the third year and in one fourth of plots in the fourth year. Wheat grown in the plots, after the fourth year, soil samples from the 0-20 cm depth of the different parts of the plots were taken and analyzed. Wheat was also harvested roots, stems and grains were separately analyzed for the heavy metal concentritons. Cumulative sewage sludge application increased OM, CEC, ECe, total and DTPA-extractable concentration of Pb and Cd in soil significantly (P≤ 0.05). Residual sewage sludge in the soil also increased CEC, total and DTPA-extractable concentration of Pb and Cd significantly. Single sludge applications at different rates increased the DTPA-extractable concentrations of heavy metals. In subsequent years with no further sludge application, DTPA–extractable metal concentrations in soil decreased continuously approaching the levels in the control. However, even after four years, DTPA-extractable concentration of Pb and Cd, were still significantly higher in plots which received more than 50 Mg ha^-1 sludge than control. DTPA-extractable concentrations of Pb was closely correlated with total concentrations. Sewage sludge increased concentration of Cd in roots and stems and Pb in grains significantly. Cumulative effects on concentrations of Pb in grains, and Cd in stems were more than residual effects. The results of this study show that cumulative and residual effects of sewage sludge application increased concentrations of heavy metals in soil and wheat.

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  Organic fertilization has been practiced in Iran due to the shortage of soil organic matter. In recent years, attention has been payed to the organic fertilizers because their commercial production has recently started and demands for their application have increased. The objectives of this study were to investigate the effects of organic fertilizer type, rates and times of application on the N mineralization potential (NMP) in a calcareous soil (fine loamy, mixed, thermic, Typic Haplargid) in Isfahan region. The soil samples were collected from a previousely established field experiment. The experiment design was split plot with three replications. Each main plot was split into subplots receiving 1, 2 and 3 annual consecutive applications of cow manure and sewage sludge at the rates of 0, 25 and 100 Mg ha^-1. The soil samples were taken from 0-15 cm depth, 6 months after the third application of the organic fertilizers. Nitrogen mineralization potential was measured by a long-term leaching-incubation procedure. Results indicated that NMP was similarly affected by cow manure and sewage sludge. Nitrogen mineralization potential in the treatments which received 100 Mg ha^-1 organic fertilizers, was 4 and 1.7 times greater than that of control and 25 Mg ha^-1 treatments, respectively. A significant increase was also observed in NMP in the treatments which received different times of application. The NMP in the three-year applied treatments was 5, 2.5 and 2.1 times greater than that of control, two- and one-year applied soils. Significant correlations were observed between NMP and corn yield (r=0.531**) and corn N uptake (r=0.568***). The product of NMP and N mineralization rate constant was also significantly correlated with corn yield (r=0.710***) and corn N uptake (r=0.734***). Different patterns were observed between the responses of total N and NMP in the treated soils.

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Root depth is one of the main parameters affecting soil moisture availability held within the plant root zone. This study was conducted to evaluate the trend of corn root development during the growing season in different irrigation methods with two water qualities, and distribution of roots in soil profile. The experiment was a split plot design with three main treatments (subsurface drip(SSD), surface drip(SD) and furrow irrigation(FI)) and two sub main treatments (municipal effluent and fresh water) in two consecutive years conducted in Korbal plain, Fars province. Root monitoring was carried out with the observation of soil profile root depth measurement. Samples were taken during the growing season 16 times, each with a 7 day interval. Measurement of root weight in different soil layers was carried out using monoliths of soil washed with water pressure using 50 mesh sieves. Although the longest roots were observed in plots irrigated with SSD and the shortest in FI, the final root length was not significantly affected by irrigation methods and water qualities. In this research, a model showing the trend of root length average growth was developed and tested in the experiment conditions. The average final main root depth for all treatments was 77 cm. Irrigation volume using root depth monitoring could be reduced compared to linear model, Borg & Grimes and CROPWAT model for root depth estimation. Irrigation water on the base of soil moisture deficit within the root zone in each irrigation event could be reduced when the real root depth is considered compared with the conventional general models giving a final root depth. Results showed the total dry root weight in SSD was more than that in SD and FI methods. However, water quality did not show a significant effect on dry root weight. The most root concentration in SSD was measured in depth of 20-40 cm, while in SD and FI the most concentration was in surface layer (0-20 cm). In the SSD, the roots were distributed more evenly compared with SD and FI methods. The concentration of roots in all treatments below 65 cm was not significant.

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Sewage sludge application on farmland as fertilizer is commonly practiced in many countries. Sewage sludge is rich in macro and micronutrients. However, high concentration of heavy metals in sludge may cause pollution of soil, groundwater and human food chain because of toxic metals uptake by crops. The objective of this study was to determine residual and cumulative effects of sewage sludge on concentration of Zn and Cu in soil and wheat. Different levels of 0, 25, 50 and 100 Mg ha-1 of sewage sludge were applied to the soil for four years. To study the cumulative and residual effects of the sewage sludge, applications were repeated on three fourth of each plot in the second year, on one half of plots in the third year and on one fourth of plots in the fourth year. Wheat was grown in the plots. After the fourth year, soil samples from the 0-20 cm depth of the different parts of the plots were taken and analyzed. After harvesting the wheat, roots, stems and grains were separately analyzed for the heavy metal concentrations. Cumulative sewage sludge application significantly (P≤0.05) increased the total and DTPA-extractable concentration of Zn and Cu in soil. Residual sewage sludge in the soil also increased the total and DTPA-extractable concentration of Zn and Cu. Single sludge applications at different rates increased the DTPA-extractable concentrations of heavy metals. In subsequent years with no further sludge application, DTPA–extractable metal concentrations in soil decreased continuously, approaching the levels in the control. However, even after four years, DTPA-extractable concentration of Zn in plots receiving more than 50 Mg ha-1 and Cu in plots receiving more than 25 Mg ha-1 sludge, were still significantly higher than control. DTPA-extractable concentrations of metals were closely correlated with total concentrations. Sewage sludge had a significant effect on concentration of Zn and Cu in stems and grains. Cumulative effects on Zn and Cu uptake by stems were more than residual effects. The results of this study show that cumulative and residual effects of sewage sludge application increased concentrations of micronutrients in soil and wheat.

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Organic fertilizers are the sources of many human-pathogenic microorganisms which potentially threaten the human health. This study was carried out to explore the possible effects of soil and manure types on filtration, transport and fate of manure-borne bacteria through undisturbed soil columns. The manure treatments consisted of cow manure, poultry manure and sewage sludge which were distributed at the rate of 10 Mg ha-1 on the surfaces of intact columns of two sandy clay loam and loamy sand soils. The manure-treated soil columns were leached by tap water with similar unsaturated flux of 4.8 cm h-1 up to four pore volumes (PV). The influent and leachate were sampled at different PVs. Gram-negative bacteria concentrations were determined for the influent and the columns’ leachate. Average influent concentration, average effluent concentration, relative filtration, and transported bacteria fraction during the leaching events were determined. Significant differences (P<0.05) were observed between the poultry manure and the other two manures in terms of average influent (i.e. manure-released) bacteria concentration. Stable structure and preferential pathways facilitated the bacteria movement in the sandy clay loam soil columns. The loamy sand soil strained 1.45 times more bacteria than the sandy clay loam soil due to its weak structure and blocked-dead pores. Relative contamination of the effluent was higher for poultry manure when compared with the other fertilizers. The low ionic strength of sewage sludge suspension caused the lower filtration of bacteria through the soil columns. The high concentration of soluble organics in cow manure resulted in a relative transport of the bacteria 1.12 times greater than the poultry manure. In general, management of organic fertilizers especially household poultry manure, as a considerable source of pathogenic bacteria, is important to control the environmental risks of pathogenic pollutions. Moreover, the soil texture and structure significantly affected the fate of manure-borne bacteria.

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Organic manures can affect phosphorus (P) sorption characteristics (PSC) and its availability in soils. Information about effect of sewage sludge (SL) on PSC in calcareous soils of Hamadan province is limited. The objective of this research was to study the effect of SL on PSC and available P in 10 calcareous soils. The soil samples were incubated with and without 1.5 % SL for 5 months at field capacity at 25 ±1 סC . After incubation, available P was determined by Olsen method. Furthermore, 2.5 g samples were shaken with 25 mL 0.01 M CaCl2 containing 0 to 30 mg P L-1 as KH2PO4. After equilibration, suspensions were centrifuged and P concentration was determined in clear extracts. The results showed that SL application increased available P. The mean available P value increased by 37.6 %. Available P in untreated and SL-treated soils ranged from 8.1 to 22.1 and 14.8 to 28.0 mg kg-1, respectively. Sorption data in the all samples were well described by Freundlich and linear isotherm, whereas sorption data in all SL-treated soils did not adequately fit to the Langmuir isotherm. The constants of sorption isotherms were lowered by SL application. The mean sorption maxima, binding energy and maximum buffering capacity values decreased by 38.85, 21.34 and 38.90 % respectively. The mean distribution coefficient, Freundlich n and the mean buffering capacity (slope of linear model) values decreased by 21.7, 16.0 and 6.1 %, respectively. The results of this study showed that application of SL decreases standard phosphorus requirement. Moreover PSC results indicated that use of sewage sludge might increase the risk of P transfer to surface water.

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Compost and sewage sludge contain high concentration of plant nutrients and, thus, have been used extensively as an inexpensive fertilizer. The objective of this study was to evaluate cumulative and residual effects of compost, sewage sludge and cow manure on nitrogen, phosphorus and potassium in soil and wheat. The experiment included compost, sewage sludge and cow manure, each applied at 3 rates (25, 50 and 100 Mg ha-1), a chemical fertilizer (250 kg ha-1 amonium phosphate + urea) and a control plot with 3 replications. The experimental design consisted in completely randomized blocks with treatments arranged in split plots. To study the cumulative and residual effects of the organic amendments, application was repeated on four fifths of each plot in the second year. Wheat was grown in the plots. The results showed that one application (residual effect) of organic amendments had not significant effect on total N in soil and wheat leaves and stem, but it led to significant increase of available phosphorus and potassium in soil and wheat leaves and stem. Cumulative effects of organic amendments significantly (P≤ 0.05) increased the total N (in 50 and 100 Mg ha-1 Cow manure and 100 Mg ha-1 Compost treatments), available P in all organic treatments and K (in all Compost and Cow manure treatments) in soil. Also, cumulative effects of organic amendments significantly (P≤ 0.05) increased the N (in 100 Mg ha-1 sewage sludge), P (in 100 Mg ha-1 compost) and K (in all organic treatments with the exception of 25 Mg ha-1 Compost) concentrations in leaves and stem.

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Sewage sludge application on farmland as fertilizer is commonly practiced in many countries. However, high concentration of heavy metals like mercury (Hg) in sludge can cause pollution of soil, plant and the human food chain. In order to examine the risk of Hg transfer into plants a five year field experiment was conducted in which we investigated uptake of Hg from a sludge-amended soil by corn. Sludge application rates were 0, 25, 50 and 100 Mg ha-1. To study cumulative and residual effects of the sewage sludge, applications were repeated on 4/5 of each plot in second year, on 3/5 of plots in third year, on 2/5 of plots in fourth year and in 1/5 of plots in fifth year. After the fifth year, soil samples from the 0-20 and 20-40 cm depths were taken and analyzed for total Hg. Corn plants were harvested and roots, stems and grains were separately analyzed for Hg concentrations. Sludge application significantly increased total Hg concentration in soil. Total Hg concentration in soil ranged from 20 µg kg-1 (in control plots) to 1200 µg kg-1 (in plots with 500 Mg ha-1 sludge application). Sludge application significantly increased uptake of Hg in different plant parts. At the end of the fifth year the average Hg concentrations in root, stem, and grain were 91, 9, and 8 µg kg-1, respectively. Corn yield increased significantly with sludge application and this fertilizer effect was visible five years after a single sludge application.

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The management and application of organic wastes in agricultural lands decreases environmental risks and increases utilization of these matters. The objective of this research was to investigate and compare the effect of polyacryl factory sewage sludge (PSS), municipal compost (MC) and cow manure (CM) on soil properties and on yield and growth of maize (single cross 704, Zea mays L.). The treatments were control (without any organic waste) and application of PSS, MC, and CM at two rates (15 and 45 t ha-1). This research was done in the research farm of Isfahan University of Technology (Lavark-Najafabad). The experimental design was a randomized, complete block with three replications. Treatments were incorporated into the soil before the maize planting (on 23 June 2008) and soil sampling was performed after 132 days (Simultaneously with maize harvesting). Application of the organic wastes resulted in significant increase soil organic matter (SOM), total nitrogen (TN) and saturated hydraulic conductivity (Ks) and significantly decreased bulk density. However, saturated hydraulic conductivity was decreased by application of PSS. Application of 15 and 45 t ha-1 of PSS reduced Ks more than 14% and 42%, respectively, compared with control however, the reduction was significant only in the plots which received 45 t ha-1 of PSS compared with control. The results of plant yield and growth showed that leaf area index (LAI), plant length, biological yield, 1000 seed weight and seed yield increase due to the application of organic wastes.

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The petroleum refinery sludge is an important source of environmental pollution. Burning and burying of the sludge may have adverse effects on environment and human health. Thus, other mechanisms for decreasing the toxic effects of hydrocarbon substances in the sludge must be used. In this study, Isfahan refinery sludge was dewatered, air dried and mixed by 0, 10, 20, 30 and 40% w/w ratio with two calcareous soils, viz., Mahmoud Abad (Typic Haplocalcids with clay texture) and Bagh Parandegan (Anthropic Torrifluvents with silty loam texture). Different mixtures of soil and sludge were farmed for 21 days and irrigated on a daily basis to field capacity. Then, 100 seeds of Tallfescue (Festuca arundinacea) and Agropyron were planted in polluted soils with 3 replicates in 3 kg pots for 5 months. Result showed that Tallfescue and Agropyron yields decreased in sludge contaminated treatments. In the 40% sludge treatment, Tallfescue decreased the total petroleum hydrocarbons content by 65 percent. The highest degradation for agropyron was in the 30% sludge treatment which showed about 55% reduction in total petroleum hydrocarbons. The 40% sludge treatment resulted in the minimum yields of root and shoot plants. The highest degradation of TPHs occurred in the Tallfescue rhizospher of 40% sludge. Maximum degradation of TPHs on the Agropyron rhizospher was in 30% sludge mixed with Bage parandegan soil, but maximum yield of plant was in 20% sludge. Our study shows that Tallfescue rhizospher is most effective for decreasing TPHs, and that the phytoremediation in soils with more clay can adsorb and fix the toxic components and then at higher levels of pollutions can let the plants grow.

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The effects of waterlogging, sewage sludge and manure on the Fe, Mn, Zn, Cu, Pb and Cd uptake and concentrations in roots and shoots of sunflower (Helianthus annuus L.) were investigated in greenhouse conditions. A factorial experiment based on a completely randomized design with three replications including duration of waterlogging at five levels (0, 2, 4, 8, 22 days) and source and amount of organic fertilizer at five levels (0, 15 and 30 grams of both manure and sewage sludge per kg of soil) was conducted. The results showed that by increasing the duration of waterlogging, the Mn uptake and concentration in shoots and Mn concentration in roots increased but Mn uptake in roots did not change significantly. The uptake and concentration of Fe in shoots and Cd concentration in shoots and roots initially increased and then decreased after soil waterlogging, while Zn and Cu concentration of roots increased after an initial decrease. The effect of soil waterlogging on the Pb, Cu and Zn concentrations in shoots was not significant. By application of sewage sludge and manure the uptake and concentration of Fe, Mn, and Zn in shoots and Cu uptake in shoots increased but the uptake and concentration of Pb in shoots increased only by application of sewage sludge. The effect of source and amount of organic fertilizer on the Cd uptake and concentration in shoots, Cd concentration in roots and Cu concentration in shoots was not significant. By application of sewage sludge the root concentration of Fe, Mn, Cu and Zn increased but root concentration of Cd and Pb did not change significantly. The maximum uptake of heavy metals in shoots was obtained by application of 30 g sewage sludge/kg of soil. The effect of soil waterlogging on the heavy metals uptake and concentrations in the roots and shoots was dependent on the source and amount of organic fertilizer. Sunflower plant accumulated Fe, Mn, Zn, Cu, and Pb in roots while the Cd concentration in roots did not differ with shoots significantly.

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Silver toxicity and its fate in the environment are currently being debated and are important as challenging research topics. Even though there are several studies on its total content in soils, fractionation of Ag especially in calcareous soils has not been investigated. Therefore, to provide fundamental information on the chemical behavior of Ag in calcareous and noncalcareous soils, we studied 8-step chemical fractions of Ag (i.e., EXCH, CARB, Me-Org, re-MeOx, H2O2-Org, am-MeOx, cr-FeOx, and RES) after 30 and 60 days of incubation in soils amended with Ag (0 and 15 mg kg-1), sewage sludge (0 and 20 t ha-1) and EDTA (0 and 0.5%). Experimental results showed that redistribution of Ag in spiked noncalcareous soils was EXCH (34%), H2O2-Org (33%) and RES (17%). In calcareous soils, after 30 days, EXCH- and RES-Ag increased but at the end of 60 days H2O2-Org-Ag increased. Based on our data, we could conclude that addition of Ag results in an increase of Ag mobility in soils but incubation and sewage sludge may have adverse effect on its mobility. In contrast to noncalcareous soil, EDTA in calcareous soil resulted in higher Ag mobility. This may have environmental implications in Ag polluted calcareous soils.

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Cow manure and sewage sludge add heavy metals to soil. Organic and inorganic fractions in these compounds can immobilize heavy metals such as lead (Pb) and affect their bio-availability. This investigation was conducted to compare the effects of organic and inorganic fractions of sewage sludge and cow manure on distribution of lead chemical forms in soil as a completely randomized design. Treatments consisted of application of 10% (w/w) enriched sewage sludge and cow manure (6 g Pb kg-1 organic amendments). Another treatment was also used in which soil Pb concentration was increased to 600 mg kg-1 soil using Pb(NO3)2 salt. To compare the effects of organic and inorganic fractions, organic carbon, iron oxide and easily reducible iron and manganese were removed from cow manure and sewage sludge, and were added to the soil by 10% (w/w). The samples were incubated at 23-25ºC for 111 days and their moisture was maintained at 80% water field capacity. More than 50% Pb in Pb(NO3)2 treatment was in exchangeable fraction, while for cow manure and sewage sludge treatments more than 40% Pb was found to be in oxide fraction. Application of cow manure and sewage sludge increased the Pb in oxide fraction by 14.8 and 17.5%, respectively. Removing organic carbon fraction of cow manure and sewage sludge increased the Pb bound to residual fraction by 12% and 14% respectively. Removing iron oxide fraction of sewage sludge and cow manure decreased the Pb bound to oxide fraction by 8% and 13%, respectively. Removing easily reducible iron and manganese decreased the oxide fraction of Pb by 16% and 14%, respectively. It is concluded that, despite relatively high amounts of organic carbon in the sludge and manure, inorganic fractions of these materials have more significant effects on availability of Pb in soil.

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In this study the effects of irrigation with raw or diluted municipal effluents along with foliar micro-nutrients fertilizer spray were examined on the growth, yield and mineral nutrient in foxtail millet plants. The experimental design was a split-plot with three irrigation sources (namely raw sewage, 50% diluted sewage and well water) as main-treatment and four combinations of Mn and Zn foliar spraying as sub-treatments, which were applied with three replications. The experiment was conducted at the Zabol University research farm during 2009. The applied municipal effluents contained higher levels of macro and micro-nutrients than the well water. The experimental results indicated that irrigation of plants by raw or diluted sewage improved the measured growth parameters and productivity of foxtail millet plants. In addition macro and micro-nutrients concentrations were improved. These improvements were attributed to the presence of high levels of essential nutrients such as nitrogen, phosphorus, and organic matters in wastewater. Manganese and Zn supplied through sewage water alone were not able to raise the productivity of millet to the level obtained through fertilizers at the recommended level, which indicated that additional nutrients through fertilizers are required to obtain higher productivity of millet under sewage farming. Despite the differences in nutrients concentration among different irrigation water sources, the micro-nutrients foliar spray did not affect concentration of macro and micro-nutrients in foxtail millet plant. Overall, the results suggest that municipal sewage could be efficiently utilized as an important source of water and nutrients in growing foxtail millet Sewage water irrigation did not have any appreciable harmful effect on crop productivity.