M. Yahyavi, Gh. Azari Takami, Gh. Vosoughi,
Volume 10, Issue 4 (1-2007)
Abstract
Stress tests are commonly applied in shrimp hatcheries to estimate the quality of postlarvae to be used during growout. The highest larval survival rate during culture and in shrimp stress tests has been reported when specimens are offered a diet containing high levels of highly unsaturated fatty acids ( HUFA ) . Also vitamin C has positive effect to resistance and survival of larvae . In this study in postlarvae stage 1 in the salinity stress tests (10 and 20 ppt ) the highest survival rate was observed in treatment 3 ( 56.667 % and 90.00 % ) which was enriched with highly unsaturated fatty acids and vitamin C . As for the second, treatment 2 ( 43.333 % and 76.667 % ) which was solely enriched with highly unsaturated fatty acids showed significant differences at a level of ( P < 0.05 ) with treatment 3 . The difference between these two treatments and treatment 1( larvae fed rotifers cultured on Chlorella sp. ) and control ( larvae fed newly hatched Artemia nauplii ) was also significant . No significant difference was observed in formalin stress test ( 100 ppm ) in this stage between treatments 3 ( 86.667 % ) and 2 ( 80.00 % ) which shows the highest survival rate . But difference between them with treatment 1( 60.00 % ) which was unenriched and control ( 53.333 % ) was significant . Also, in postlarvae stage 5 in the salinity stress tests (10and20 ppt) the highest survival rate was observed in treatment 3 ( 56.667 % and 83.333 % ) and later treatment 2 ( 40.00 % and 70.00 % ) which showed significant difference . In this stage , the survival rate the treatment control ( 60.00 % and 86.667 % ) and treatment 3 showed no significant difference but they showed significant differences with treatment 1 and 2 .The lowest survival rate was observed in treatment 1( 26.667 % and 56.667 % ) . And the highest survival rate was observed in formalin stress test ( 100 ppm ) in this stage in treatments control , 3 and 2 ( 76.667 % , 73.333 % and 70.00 % ) among which there were no significant differences . However, the difference between these three treatments with treatment 1 ( 53.333 % ) was significant .
A Bahri, Gh Azari Takami, A Keyvan, Gh Vosoghi,
Volume 13, Issue 48 (7-2009)
Abstract
Immunostimulants are one of the important ways to prevent diseases. The vaccination and immune system stimulator and their combination can increase the ability of vaccines in prevention of diseases and improved indexes of growth and survival rates in shrimps. In this research, the separate and simultaneous effects of Ergosan and Vibromax vaccine on the growth factors such as total length and increasing of dry weight and survival rates in three stages of post larvae PL1,PL5 and PL15 in Indian white shrimp (Fenneropenaeus indicus ) were studied. The feeding of vaccine to shrimps was done through Artemia nauplii ( Artemia franciscana) enrichment. The effects of Ergosan (T1) and effect of Vibromax (T2) separately and in combination (T3) along with a control treatment for comparison were studied. Taking into consideration of 4 treatments and 3 replications for each of them, 12 similar vessels were used, which were refilled with 10 liters of water and reserved with 100 larvae in liters in zoa stages and were fed in a completely randomized design. Duration of experiment was from zoa stage to PL15 and in the end of 12, 16 and 25 days they were analyzed for the biometry and determination of survival rates. Results showed that the highest amount of total length (mm) was observed in PL1,PL5 and PL15 stages in T3 (5.26, 6.45 and 18.41) and was a little different from that in T1 (5.28, 6.32 and 17.94) which was significantly different from the control treatment ( 4.91 , 6.09 and 17.36 ) at P<0.05 level. Furthermore, T2 (5.11, 6.07 and 17.44) and control treatment were not significantly different (P>0.05). The highest amount of dry weight (mg) was observed in PL1, PL5 and PL15 stages in T3 (0.296, 0.890 and 2.940). In contrast to the control (0.216, 0.640 and 2.875) it was significantly different (P<0.05).The highest amount of survival rates (%) was observed in T3 (77.33, 76.33 and 80.67) which unlike control treatment (57, 57.33 and 59.67) was significantly different (P<0.05). The use of these two products in a desired feeding program from zoa stage to PL12 could increase the resistance and immunity in post larvae and survival rates and growth factors in shrimps leading to produce suitable post larvae for introducing into the training ponds.
