JWSS - Journal of Water and Soil Science

In addition to the distribution of elements in the soil solid phase, element species in the solution are also very important due to their importance of providing elements for root uptake. For a deeper study of the chemical cycle of elements in saline soils treated with biochar, the study of speciation is very useful and provides a method to reduce or transform the toxicity caused by toxic elements in saline soils. Therefore, to investigate the effect of biochar on Cd speciation in two saline calcareous soils, 15 mg kg^-1 Cd as cadmium chloride was added to the soil sample (200 g), and the soils were incubated for three weeks at 25±2 °C at 80% field capacity. After the incubation period, salinity levels of 20 and 40 mmol kg^-1 as sodium chloride (equal to 3.65 and 7.30 dS m^-1) were added to the soils. Then, the 1% (w/w) of the sugarcane bagasse and biochars produced at 400 and 600 °C were added to the soils, and then incubated for three months at 25±2 °C at 80% field capacity. At the end of the incubation period, for the speciation of Cd in the soil solution (in a 1 to 2 ratio), the concentration of dissolved cations and anions in the soil samples was measured. The results showed that the interaction between salinity, biochar, and soil on Cd²⁺, CdCl⁺, CdCl₂⁰, and Cd(SO₄)₂^2- was significant. The application of biochar in sandy soil reduced (p <0.05) the concentration of CdCl⁺, CdCl₂⁰, CdSO₄⁰, and CdOH⁺ species compared to the control soil, while it did not affect clay soil. Also, salinity caused by sodium chloride in sandy soil increased the concentration of CdCl⁺ and CdCl₂⁰ species and decreased CdSO₄⁰ and CdOH⁺ species compared to the control soil (p <0.05). The results showed that biochar in saline sandy soil was more effective than clay soil in reducing Cd toxicity.

This study aimed to evaluate the effectiveness of iron (Fe) aminochelate application methods on Fe chemical speciation in the soil solution, as well as Fe concentration and uptake in sunflower seeds (Helianthus annuus L. cv. Oscar). The experiment was conducted in a randomized complete block design with three replications at the research field of Shahid Chamran University of Ahvaz. Treatments included two application methods (seed priming and fertigation) and three Fe sources: Fe–glycine aminochelate [Fe(Gly)₂], Fe–methionine aminochelate [Fe(Met)₂], and ferrous sulfate (FeSO₄·7H₂O), along with an unfertilized control. Fe speciation was determined using Visual MINTEQ software. Results indicated that Fe aminochelates, [Fe(Met)₂], significantly decreased soil pH and increased DTPA-extractable Fe (by 35.7%), seed Fe concentration (by 13.5%), and seed Fe uptake (by 79.1%) compared with the control (p < 0.01). Application of Fe fertilizers also significantly enhanced the concentrations of dominant Fe species (Fe²⁺ and FeSO₄(aq)) in the soil solution, with the highest Fe²⁺ level (3.1-fold higher than the control) observed under [Fe(Met)₂] seed priming. Strong and significant positive correlations between Fe²⁺ and FeSO₄(aq) concentrations and both DTPA-extractable Fe (r = 0.88** and r = 0.89**, respectively) and seed Fe uptake (r = 0.84** and r = 0.87**, respectively) highlight the pivotal role of these species in improving Fe bioavailability and uptake by plants in calcareous soils.