JWSS - Journal of Water and Soil Science

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Physicochemical characteristics of potato (Solanum tuberosum L.) are very important for storage and processing qualities. The amount of reducing sugars (glucose and fructose) and organic acids at harvest, during and after storage is the most important factor. In this study, three potato cultivars (Moran, Marfona and Agria) were collected, dry cleaned and graded into three sizes. They were analyzed for specific gravity and dry matter content. Potato tubers were stored at 4°C and about 85% R. H. for 15 weeks and then reconditioned at room temperature (25±l)°C for 4 weeks. During the storage period of tubers, sugars (sucrose, glucose and fructose) were analyzed on a weekly basis, but citric, D-malic and D-pyroglutamic were analyzed on a monthly basis by high performance liquid chromatography (HPLC). Data were analyzed statistically in a completely randomized design with sampling and Duncans multiple range test.

The total sugar and organic acid contents of tubers significantly increased during the 15 weeks of storage at 4°C and decreased after reconditioning at room temperature. The size of tubers did not affect the accumulation of total sugars. Dry matter content and specific gravity of Agria tubers were higher than those of both Moran and Marfona. Also accumulation of reducing sugars was lower in Agria than in Moran and Morfona initially and after the storage and reconditioning periods. Therefore, Agria must be suitable for processing into chips or dry powder.

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Molasses are produced in large amounts by sugar cane and sugar beet factories every year. However, they are considered as the waste product of the crystallization plants. Thus, although they contain significant amounts of materials with deep black colour which can be separated as food colour, molasses are very cheap and feasible to be used for this purpose. Aliquots of diluted molasses, after adjusting the pH, were passed through the column followed by extraction with acidified alcohol. The highest yield of extraction (76.3%) was obtained by using diluted molasses (10° BX) and 150 ml of Amberlite resins. The solvent was removed and the solid colour was obtained by evaporating the water using a freeze drying system.

 The colour was characterized by determining its solubility, amount of ash, and sugar content and its colouring ability. Also, the stability of the colour under different conditions of acidic pH, temperature, and visible light was investigated. In addition, the colour values of the colour solution were measured by Hunter lab system.

The colour was compared with the imported caramel which is used in the black soft drinks of Zam Zam. Both had almost the same values of λ_max. Also, their sensitivities to pH changes were similar. Samples of soft drinks made by using the prepared colour were compared with those made by caramel under different conditions of storage such as temperature and light. On the whole, the colour was found to be a very suitable replacement for caramel in the production of black soft drinks.

 Also the colour was used as partial replacement for cocoa powder in making chocolate milk. The taste panel showed that cocoa powder can be replaced by the colour powder by 20% with almost no detectable differences. The caramel from molasses is safe and can be used as a food colour. The cost of production was estimated at about 10 Rials per gram of caramel. Thus, from an economical point of view, industrial production of caramel from molasses is profitable.

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The main sources of cadmium in soil-plant continuum in amounts that might present a hazard are liquid and solid wastes of sewage sludge, farm manures and fertilizers. In the southwest of Iran (Khuzestan Province) over 50,000 ha of land is under sugarcane (Saccarum officinarum) cultivation and more than 80,000 ha will be under sugarcane by the end of the year 2000. In these sugarcane fields, about 400 kg ha^-1 diamonium phosphate (DAP) and 400 kg ha^-1 urea are applied annually. There is no data available to show the fertilizers impact on soil, water and plant contaminations in Iran with respect to cadmium. The objective of this research was to compare the extractable cadmium of virgin soils with that of soils under sugarcane.

 Four sugarcane growing stations viz. Haft-tapeh, Karoon, Shoeibieh and Ghazali with cultivation histories of 36, 20, 2 and 1 year, respectively, were selected. In each site, along a transect soil samples from 0-30 cm of both furrows and ridges of cultivated soils and of virgin soils were collected. Electrical conductivity (EC), pH, clay and organic carbon contents, CI and Cd of 101 soil samples were measured according to standard methods. Results showed that increasing either EC or CI increased Cd concentration with its maximum in virgin soils and its minimum in furrows.

 Results also indicated a slight decrease in the Cd content of cultivated soils.

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Field studies of S. cretica Led. damage and potential crop losses on two important cultivars of sugarcane, NCo 310 and CP 57-614, were carried out in southern Khuzestan in 1998-1999.

 CP 57-614 showed greater mean percentage of bored nodes and internodes (20.7%) than NCo 310 (11-2%). Apart from the direct losses in cane weight (cane yield) due to boring from the larvae, cane juice quality is also adversely affected, resulting in lower recovery of sucrose in the factory. Sugar per ton of stalks also was greater in NCo 310 (132 kg) than in CP 57-614 (110 kg). Estimated sugar losses were 0.11 tons ha-1 for every 1% bored and rotten stalks. Other indirect losses are also discussed.

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A glasshouse study was conducted to measure the effects of salinity and drought stresses on growth and chemical and biochemical composition of 4 onion cultivars. The cultivars were Dessex, Texas Early Grano (Texas), Dehydrator, and PX492. Salinity treatments included control, 45mM NaCl, 45mM NaCl + 5mM CaCl2 and drought treatments were control (maintaining soil moisture at field capacity) and irrigation when 50% of available water was used. Four weeks after the treatments, the plants were harvested and root and shoot dry weights (RDW, SDW), Na⁺, K⁺, Ca²⁺, total protein, reduced sugars, as well as free proline contents were measured in both roots and shoots.

 Results showed that NaCl and drought treatments significantly reduced SDW and RDW. The Texas cultivar and the Dessex cultivar produced the highest and the lowest amounts of SDW, respectively. NaCl significantly increased Na⁺ uptake but reduced K⁺ uptake in shoots and roots and also reduced Ca²⁺ uptake in roots. NaCl+CaCl₂ significantly alleviated the deleterious effects of NaCl such that SDW significantly increased in two cultivars and increased RDW and the K⁺ contents while causing decreased Na⁺ and sugar contents in shoots and roots of all cultivars. All stresses increased total protein contents of shoots in Texas only but decreased or had no effect on others. Root total protein increased under salinity stress, while drought had no effect. Changes in proline and sugars in both shoots and roots did not follow any particular pattern. Out of the biochemical compositions measured, shoot total protein in plants under the stresses showed a positive significant correlation with SDW, which may be used to screen onion cultivars for drought and salinity stresses.

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Pectin is used in a number of foods as a gelling agent, thickener, texturizer, emulsifier and stabilizer. Sugar-beet pulp, the residue left from sugar extraction, is a rich source of pectin. It was the purpose of this study to develop a procedure for the extraction of pectin from sugar-beet pulp and to improve the functional properties of this pectin for more potential uses. To extract pectin, pressed beet pulp was dried and powdered. Then, it was extracted under various conditions (pH 1 and 1.5, temperatures of 80^oC and 90^oC and extraction periods of 1, 2, 3 and 4 hr). The highest yield (22.35%) was obtained at 90^oC after 4 hr at pH 1. Chemical composition of the extracted pectin and that of commercial citrus pectin were determined and compared. Also, the beet pulp pectin was used in certain foods and its functional properties were compared with those of the commercial citrus pectin. The effects of ammonium persulfate and high pH levels on the above mentioned properties were investigated. Finally, the effects of the beet pulp pectin and the commercial citrus pectin on the flavor of certain foods were evaluated. The results indicate that the extracted beet pulp pectin can be used in certain foods such as ketchup sauce as a thickener or as an agent increasing the viscosity however, it does not have the ability to form firm gels in food.

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Experiments were carried out in order to micropropagate sugarcane cultivars through shoot tip and auxilliary bud culture. Rinsing of four cultivars of sugarcane, namely CP-48-103, CP-57-614, CP-69-1062, and NCO-310 in 75% alcohol for 60 seconds and their subsequent disinfection with sodium and calcium hypochloride (1.5% active material) for 15 minutes decreased a significant amount of infection of explants in the medium. The use of the Murashing and Skoog (MS) solid and liquid medium with 1 mg/l Indole Butyric Acid (IBA), 1 mg/l Kinetin, 100 mg/l mio-inositol, 1 mg/l Thiamin HCl, and 2% sucrose had significant superiority (P<0.05) to 1/2 MS solid medium. Also, to increase the multiplication in a sterile medium (In vitro), two kinds of solid and liquid MS medium, with a hormone combination of 1 mg/l IBA, 2 mg/l Kinetin and 1 mg/l 6-(benzylamino)-9-(2-tetrahydropyranyl)-9H-purine (BAP) were applied which yielded the highest amount of proliferation. The plants formed roots in Schenk and Hildebrandt (SH) medium with a hormone combination of 5 mg/l IBA and 1 mg/l Kinetin. When activate charcoal was used in the medium, a higher percentage of the plants became rooted and a larger number of adventitious roots were produced than in the dark-light or light treatments.

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OF2 and VAP genes, probably involved in signal transduction of sugarbeet nematode resistance, have already been cloned in bacterial vector by AFLP molecular marker and a two-hybrid system, respectively. To examine their capability to introduce resistance in sugarbeet, the genes were transferred to plant expression vectors. For this reason, OF2 gene after isolation was inserted within T-DNA of pAM194 binary vector, downstream of CaMV35S constitutive promoter and also inserted within T-DNA of modified pBin121 binary vector, downstream of HS1^pro-1 gene inducing promoter (responsible for nematode resistance). VAP gene after isolation was inserted within T-DNA of pAM194 plasmid, downstream of CaMV35S constitutive promoter. Thus, three new constructs were made in which genes of signal transduction pathway were expressed to give beet cyst nematode resistance. These plasmids were separately transferred to Agrobacterium rhizogenes, strain AR15834. In the next step, petiole explants of sugarbeet were inoculated with the bacterial cells. Transformation-derived hairy roots were analyzed by GUS staining and/or PCR and were then inoculated with nematode larvae. Primary results showed partial resistance against nematode larvae in some hairy roots. As a result, this resistance can be related to OF2 and VAP genes effect.

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The changes of main sugars and organic acids of Red delicious and Golden delicious apples from Semirom region in Isfahan Province were determined by high performance liquid chromatography (HPLC). The apples were harvested, graded and packed in three kinds of wood, car-board and perforated polyethylene film packages and stored for eight months at 0±0.5^oC and 90 to 95% relative humidity for further analysis. Sucarose, glucose and fructose, as well as citric and L-malic acids were detected and quantified in all packed samples. The obtained data were analyzed with factorial experiment in completely randomized design and mean were compared by Duncan^,s muliple range test. The results showed that fructose was dominant in both cultivars in all packages compared to other sugars. Golden delicious apples contained more sucrose and less glucose than Red delicious. All sugars decreased in packed apple samples during the storage period. The dominant organic acid of both cultivars was L-malic. Citric acid increased to its maximum level in Red or Golden delicious after one or two months of storage, respectively, and then decreased gradually by the end of storage period. In general, apples kept in perforated polyethylene films exhibited the best physiochemical properties during eight months of cold storage.

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The first Iranian hybrid monogerm cultivar of sugarbeet was developed in 2000 after an 8-year period of R&D activity. The cultivar is not only a high yielding variety but enjoys a good resistance to bolting and Sercospora. The study sought to estimate the rate of return to the public investment made to develop this variety, using the economic surplus approach. The results revealed that the investment has got an internal rate of return of 117 percent and that it has produced an exchange saving of $28.7 million. This finding indicates that the investment has been highly productive and that the field suffers underinvestment.

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This study was conducted in a sugar beet field at Collage of Agriculture Experimental Station, Ferdowsi University of Mashhad, Iran. In order to describe the pattern of spatial variations and density of Chenopodium album, Solanum nigrum, Amaranthus sp., Portulaca oleracea, Echinochla crus-galli, and Convulvulus arvense as the main prevalent annual and perennial weeds of sugar beet fields, geostatistic methods were used. Samples were taken by systematic method from the corners of (7m × 7m) grids, using (0.5m × 0.5m) quadrates in three stages (before application of herbicides, after herbicide treatment, and before harvesting sugar beets). The integrity of spatial variation of variables was determined by using variogram functions and distribution maps of species. The variograms indicated that variations of all variables did not happen by chance. The maximum and minimum ranges of variation were observed in Solanum nigrum (by 142.7m) and Portulaca oleracea (by 1.5m), respectively. Both maximum and minimum ranges of variations were related to pre herbicide application. The highest and the lowest spatial correlations were related to Amaranthus sp. (in the third sampling treatment) and Solanum nigrum (in the first stage of sampling), respectively. The spatial distribution maps confirmed the patchiness distribution of the weeds. The patch of weed was constructed from a dense point at the center, gradually tapering toward the edges. The patches were skewed across the rows and irrigation channels. The structure of patches altered during the growing season. Any information on the distribution of weeds in the fields can be useful to improve decision makings in relation to applying the herbicides, selecting the herbicide type or applying the amount of herbicide. Also it can be useful to better design of weed control programs.

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Genetic male sterility is controlled by one pair of ressesive allele (aa) in sugar beet. This trait is used in most breeding programes. The exsistance of the character in a line or population facilitates transfer of important trait to the breeding material (for example resistance to plant disease). Also, it is possible to increase genetic diversity of monogerm populations by using genetic male sterility. The time and cost of transferring of this gene will be decreased, if the character is tagged with a molecular marker. Bulked segregant analysis using 302 RAPD primers in two F2 populations (231 and 261 population) was performed for the the identification of RAPD markers linked to the genetic male sterility gene. DNA preparation from 8 male fertile and male sterile plants were separately mixed. At first, the primers were tested on bulks. The primers with polymorphic bands were tested on individual plants of the bulks. Only if the polymorphism of the primers was confirmed, they were tested on the other individual plants. Finally, 10 and 6 markers were identified in 231 and 261 populations, respectively, which their distances to male sterility gene were lower than 50 cM. AB-8-18-600r marker was the nearest marker to male sterility gene. This marker showed only 3 and 1 recombination in 231 and 261 populations, respectively. The distance of this marker and genetic male sterility locus was estimated as 5.3 cM in combined F₂ populations.

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Sugarcane (Saccharum officinarum L.)‌ and rotational crops have been cultivated without potassium fertilizers for many years in southwestern Iran. Although potassium was removed from this soil, no response has been reported to K fertilizers by crops. This study was conducted to evaluate the effects of wetting and drying, and cultivation systems on potassium fixation in some Khouzestan soils. The results showed that expandable clay mineral was observed in cultivated soils but not in the non-cultivated soils. This may be related to irrigation, and cultivation practices in this soils. By adding K and sequential wetting and drying the amount of K-available increased in sugarcane, rotational cropping and uncultivated soils from 132.6, 226.2 and, 171.6 mgkg^-1 to 266, 447 and 628, respectively. These results showed that more K can be fixed after cultivating soils, especially by sugarcane, but available K increased by adding K. All surface soils had higher K fixation capacity than subsurface ones. Also, by application K to these soils, the amount of K fixation decreased with increasing wetting and drying times. The K fixation increased by the increase of cation exchangeable capacity. Significant difference was observed between cultivation system and depth of sampling in 1% levels. High K fixation can be attributed to illite minerals depleted from K. Mica-like minerals formed after adding K, and wetting and drying cycles. Amount of K fixation by clay particle samples was more than the same soil samples but in both the same trend was observed for K fixation. Also, drying and wetting decreased K fixation in the last periods. It may be due to trapping K in the interlayer positions.

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The present research was intended to alleviate the problems and costs of deep tilling in sugarcane production in Mian-Ab sugarcane farms located in Khuzestan province, Iran. The main objective was to investigate the feasibility of subsoiling operations with bentleg subsoiler (BLS) equipped with shallow tines, a combination expected to increase the critical depth resulting in less power consumption and improved soil physical conditions. Six treatments arranged in a completely randomized block design were used to measure the draft force (except for the bulldozer mounted rippers), disturbed soil cross-sectional area and penetration resistance. The treatments included (1) subsoiling with rippers mounted on bulldozer, (2) BLS without attachment, (3), and (4) BLS equipped with single tine at working depths of ½ and ⅓ of target depth, (5) and (6) BLS equipped with two tines at ½ and ⅓ target depth. The experiment was replicated three times. The results indicated that BLS without attachment had the highest draft requirement as compared with other BLS treatments with shallow tines. In general, tine attachment resulted in less draft mainly due to displacement of critical depth to lower soil zones. Increasing the number and depth of shallow tines resulted in less draft force. The BLS equipped with two tines working at ½ target depth exhibited minimum drawbar power. The inclusion of shallow tines resulted in 21% reduction in drawbar power requirement as compared to BLS without tine attachment. The cross-sectional area of the soil tilled by ripper was minimum. Furthermore, the tilled area was not uniform and subsequent subsoiling operations were needed. The average soil cross-sectional area per unit width was improved by BLS treatments by a factor of 2.3 as compared with that of ripper. The highest cone index was obtained in plots tilled by ripper the BLS reduced this index in a significant manner. The soil worked with subsoiler equipped with tines exhibited the least resistance. The range of wheel slippage for BLS treatments was 12-16% which lies in the upper end of the recommended range for optimum traction efficiency. The proposed idea proved prominent in subsoiling fine textured compact soils.

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  Sugarcane is one of the most important sugar crops in the world. Because of semi-arid climate and salinity of its cultivation area in our country, increasing salt tolerance of sugarcane is signifying. To achieve this goal determining salt tolerant cultivars and understanding salinity mechanisms in sugarcane are very important. This study was conducted to evaluate 8 commercial and promising sugarcane cultivars at early stage of growth. A complete randomized design with three replicates and four salinity treatments (0, 0.25, 0.5, 0.75 % NaCl) was used in a hydroponics system. The effect of salinity on absorption, transport and accumulation of Na⁺, Cl^- , K⁺ and Ca²⁺ ions in shoot and root was determined. At high level salt concentration, Cl^- content in shoot and root increased. Result showed that sodium accumulation in sugarcane plants was more than potassium. By increasing salinity level, sodium uptake and its translocation to shoots increased reducing growth and dry matter yield of plants. With rising salt concentration from medium (0.5%) to high (0.75%), content of chloride in shoot and root of NCO-310 was constant showed that this cultivar had genetic ability to avoid Cl^- uptake. CP82-1592 with lowest ratio of shoot / root chloride had minimum transport of Cl^- to shoots. Also this cultivar had high content of Ca²⁺ in shoot and low Na⁺/Ca²⁺ ratio at all salinity levels. CP48-103 had low sodium in shoot and relatively low sodium in root. Thus it probably has genetic potential to avoid sodium uptake. At last, exclusion of Na⁺ and Cl^- to older leaves and tillers was seen in CP82-1592 and CP72-2086 cultivars. According to results, to avoid once of absorption and transport, and exclusion of harmful Na⁺ and Cl^- ions were mechanisms that could be used in salinity tolerance of sugarcane.

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  In this research, after optimization of sterilizing cyst and larvae of second stage of Heterodera schachtii, the possibility of using nematode on seedlings of sugar beet (Beta vulgaris L.) in in vitro condition was studied for developing larvae to cyst. For this purpose, non sterile cysts were extracted from infected soil and hatched into zinc chloride solution with concentration of 0.5gl^-1. Then, for preparation of sterile second stage larvae, several sterilizing treatments were used . Mean comparisons were performed between sterilized live larvae number by Duncan's method. Results showed that 70% ethanol for 1 minute followed by 5% sodium hypochlorite for 5 minutes and 0.1% sodium hypochlorite for 20 minutes were the best treatments for disinfecting cysts and larvae, respectively. In the next step, two nematode susceptible sugar beet varieties were applied to produce cyst from the larvae in in vitro culture. PG_oB medium containing different hormonal compositions was used to produce hairy roots and inoculation of seedling with sterilized larvae. After nematode inoculation tests, were the stained cysts were observed under stereomicroscope and counted 40 days later. Five to twelve cysts were formed on the roots of each seedling from two varieties. As a result, it seems that this technique can be used for sugar beet germplasm evaluation to screen nematode resistant genotypes in in vitro controlled condition.

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Due to yield increase, some farmers in Iran plant sugarbeet in 50-cm row spacing instead of conventional 60-cm row spacing. Low row spacings force farmers to harvest three consecutive rows instead of two alternate rows. This would increase the amount of draft requirement to pull the lifter through the soil. In order to use common medium tractors for pulling the three-unit sugarbeet lifter and properly lifting the sugarbeet tubers out of the soil, applying vibration to the shanks of the lifter was taken into considertion. In this study, the effects of vibration frequency and share rake angle of a vibratory lifter on its performance were investigated. Draft, slippage, percentage of broken and non-harvested tubers were determined for four vibration frequencies (0, 9, 10 and 12 Hz) and three share approach angles (11, 24 and 36 deg.), using a factorial experiment arrangement in a randomized complete block design with three replications. The results showed that the variations in draft and slip with frequency and rake angle were similar. Although the minimum value of draft resistance was obtained at 24 deg. of share rake angle with non-vibrated shanks, 50 percent of tubers remained in the soil and were not harvested. However, the non-harvested tubers reduced to only 20 percent when vibration was applied to the lifter. So using the vibrating shanks improved the removal of the tubers out of the soil. A ratio (K) of draft to the harvested tubers (whole and broken tubers) was defined for selecting the optimum combination of the rake angle and vibration frequency. The K ratio was calculated and analyzed for different combinations of the rake angle and vibration frequency. K ratio comparison showed that, for minimizing percentages of broken and non-harvested tubers, the sugarbeet lifter should have share rake angle of 24 deg. and vibrate with frequency of 9 Hz.

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Cultivars or species of different plants through physical and biochemical characteristics or indirectly through food diet of host plant can influence behavioral characteristics and efficiency of a natural enemy. This investigation was conducted to determine percentage of parasitism and functional response of parasitoid wasp, Platytelenomus hylas (Hym., Scelionidae) to different egg densities of stem borer, Sesamia nonagrioides (Lef.) and effect of different cultivars on it. At the first stage, eggs of pest were separately collected from fields in three cultivars of CP48-103, CP69-1062 and CP57-614 and then percentage of parasitism in each cultivar was calculated. For determining functional response of the wasp, collected eggs from fields were reared to adult stage at 29±1 ºC, 60% of RH,. Then functional response of wasp was investigated at densities of 2, 4, 6, 8, 14, 20, 30, 40, 50, 60, 70, 80, and 90 of the host eggs for 24h. Analysis of data was done using SAS software. Results showed that egg parasitism by P. hylas in cultivars were significantly different (p<0.05). Type of cultivars influences rate of egg parasitism. Comparison of means by Duncan’s test showed that CP48-103 cultivar significantly differed from two other cultivars: CP69-1062 and CP57-614. Functional response in three cultivars was found to be a type III. Comparison of estimated parameters by Holling model in three cultivars showed that searching efficiency on CP48-103 cultivar was significantly higher than two other cultivars but CP57-614 and CP69-1062 were close to each other. Also, handling time (Th) and the maximum rate of estimated parasitism (T/ Th) on the three cultivars were different and on CP69-1062 they were significantly less than the two other cultivars.

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In order to investigate the effect of sugar plant effluent on shoot solute percentage, yield components and grain yield of two wheat cultivars, a two year field experiment was conducted on a farm near Eghlid sugar plant during 2004-2005. Treatments consisted of two wheat cultivars (Alamot and Zarin) and two irrigated treatment: irrigation with effluent and irrigation with spring water (control). The statistical design was a completely randomized factorial with three replications. The results showed that under effluent treatment, nitrogen, phosphorus and calcium percentage increased in shoot, and Fe, Mn, Zn, Cu and B concentration decreased. However, effluent treatment had not any significant effect on K, Mg and S concentration. The results showed that N percentages in Alamot and Zarin cultivar under control treatment were 2.41 and 2.54% and under effluent treatment were 3.28 and 3.41%, respectively. P percentages under control treatment were 0.42 and 0.47% and under effluent treatment were 0.46 and 0.51%, respectively. Ca percentages under control treatment were 0.29 and 0.32% and under effluent treatment were 0.46 and 0.51%, respectively. In both years, the lowest number of tiller, number of spike, number of kernel per spike, thousand kernel weight, grain yield and harvest index were obtained from effluent treatment in the two wheat cultivars whereas this reduction was higher in Alamot than Zarin cultivar. In Alvand and Zarin cultivars, the lowest number of tiller per plant with 2.33 and 2.50, number of spike per plant with 1.83 and 1.92, number of kernel per spike with 31.67 and 32.50, grain yield with 5233 and 5532 kg ha^-1 and harvest index with 32.03 and 33.53% and water productivity with 0.72 and 0.75 kg m^-3 were respectively obtained from effluent treatment compared to control. Thus, the results showed that using sugar plant effluent could decrease grain wheat quality and wheat grain yield.

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Sweet tasting glycyrrhizic acid is considered to be the most important chemical compound in licorice root. However, environmental factors affect chemical composition of licorice root including glycyrrhizic acid content. In this study, the effects of the harvesting time and location on licorice root composition were studied. Chemical composition of the licorice roots (Glycyrrhizia glabra) collected in summer and fall seasons from three locations in Fars province (Eghlid, Beiza and Doshman-Ziary) and one location from each of Kerman (Baft) and Kermanshah (Islam-Abad) provinces was determined. Glycyrrizhic acid, ash and sugar content before and after hydrolysis were measured in all samples after extraction. A combined analysis of variances was performed on data based on a randomized complete block design in six replications. The results showed that effects of harvesting season on sugar content, and the effects of location and its interaction with harvesting season on ash, sugar and glycyrrhizic acid content were significant (p<0.01). It was also found that the lowest ash content were obtained from roots harvested from Islam-Abad in summer the highest sugar content before hydrolysis belonged to roots harvested from both Baft and Islam-Abad in fall. Highest amount of sugar after hydrolysis was reported in the roots collected from Islam-Abad in Fall. Roots harvested from Beiza in Fars province in fall had the maximum glycyrrizhic acid content.