JWSS - Journal of Water and Soil Science

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Land application of sewage sludge is potentially beneficial as an inexpensive nutrient source. However, problem with the use of sludge may exist from high soil concentrations and subsequent uptake of heavy metals by plant and entering of the metals into the human and animal food chains. A field study with lettuce (_{Lactuca sativa} L.) and spinach (Spinacia oleracea L.) was conducted to examine uptake of heavy metals from a sludge amended soil as affected by sludge rate and time of sludge application. Sludge rates were 0, 22.5, and 45 ton/ha. To determine the effect of time on heavy metal uptake, a year after the first plants were harvested, one third of each plot was planted without sludge application and to the rest of each plot sludge was added in the same rates as before. Total and EDTA-extractable Cu, Zn, Pb, and Cd concentrations in soil were determined. The metal concentrations in shoots and roots of the plants were determined separately. Total metal concentrations showed an increasing trend with addition of sewage sludge. Copper, Zn, and Pb EDTA-extractable concentrations in soil and concentrations of these metals in the plants increased significantly with sludge rate. Time of sludge application did not have any significant effect on EDTA-extractable and plant uptake of metals. Sewage sludge also increased the crop yields significantly.

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Iron (Fe) availability is low in calcareous soils of Iran due to high pH levels and presence of excessive amounts of CaCO₃. Overfertilization by phosphorus (P) fertilizers may also decrease Fe availability. The objective of this study was to evaluate the effects of P, Fe and their interactions on the growth and chemical composition of soybean [Glycine max (L.) Merrill] under greenhouse conditions. Treatments consisted of a factorial arrangement of P rates (0, 40, 80, 120 and 160 mg kg^-1 as KH₂PO₄) and Fe rates (0, 2.5, 5 and 10 mg kg^-1 as FeEDDHA) in a completely randomized design with four replications. Plants were grown for 8 weeks in a loamy soil, classified as Chitgar series (fine-loamy, carbonatic, thermic, Typic Calcixerepts). Results showed that P application up to 80 and Fe at 2.5 mg kg^-1 increased shoot dry matter. Phosphorus concentration, total uptake and P:Fe ratio in soybean increased by P application but decreased by Fe application. Application of Fe up to 2.5 mg kg^-1 increased dry matter but decreased it at higher rates. Concentration and total uptake of Fe increased by Fe application but decreased by P application. Interaction of P and Fe had no effect on shoot dry matter. Zinc (Zn) and copper (Cu) concentrations decreased significantly when P was added and manganese (Mn) concentration increased up to 40 mg P kg^-1 but decreased at higher rates. Iron application had no effect on soybean Zn and Cu concentrations but decreased Mn concentration at all rates. Prior to any fertilizer recommendations, it is necessary to study the effects of P, Fe and their interactions on soybean under field conditions.

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A great number of studies have been conducted on the role of Azotobacter - a free living N₂-fixer - in agricultural soils, but our information is lacking on the importance of Azotobacter in pasture soils. This research was conducted to study the relationship between ecological and edaphical factors with the population of the bacteria in soil. Soil samples were taken from two sites in Azarbaidjan (Mishou-dagh and Khadjeh), having different ecology, soils and vegetation types. We investigated also the effect of inoculation of rhizosphere with soil-isolated A. chroococcum on the growth of wheat (Triticum aestivum L. cv. Omid) during 56 days of growth in a pot experiment. The treatments included: 1) inoculation with A. chroococcum (+A), 2) N fertilizer at a rate of 35 mg/Kg as (NH₄)₂SO₄ (+N), 3) +A+N and 4) –A–N as control with four replications. Results showed that the population of Azotobacter chroococcum correlated positively with organic carbon and negatively with soil pH. In soils covered with vegetation the population of bacteria was higher than that in uncovered soils and the highest population of bacteria was found in soil samples collected from the rhizosphere of grass species and the lowest in that of legume species. In inoculation experiment, shoot and, particularly root growth, increased in the order of +N, +A and +A+N in comparison with control (–A–N) plants. These treatments also stimulate uptake and transport of K into shoot. Interestingly, the effect of +A treatment was significantly higher than that of +N and +A+N treatments. The same results were obtained for nitrogen. A higher uptake and particularly transport of K into shoot was attributed to low molecular weight organic molecules produced in inoculated roots and transport K into shoot. Results showed that inoculation with Azotobacter not only enhanced growth and chlorophyll content, but also stimulated uptake and translocation of ions into shoot. The stimulatory impact of inoculation is most likely a general effect that could also be important in micronutrients translocation into shoot.

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Potato (Solanum tuberosum L.) has relatively weak root system and requires high nitrogen fertilizer which is costly and may pose environmental pollution. This study was conducted to compare root morphological characteristics and nitrogen uptake of some potato cultivars growing in Iran. A greenhouse experiment using a completely randomized design with 3 replications and 8 potato cultivars including: Arinda, Agria, Premiere, Diamant, Concord, Marfona, Marodana and Nevita was conducted at Isfahan University of Technology, Isfahan. Virus-free seed tubers were planted and grown up to flowering stage. The plants were then harvested and their root length (RL), root average diameter (RAD) and root surface area (RSA) were determined using a Delta-T Scan image analysis system and Windias software. Root length density (RLD), root length and shoot weight ratio (R/S) were also calculated. Dry mater production and nitrogen accumulation (total nitrogen uptake) (PNA) were also determined. Soil inorganic nitrogen was measured before planting and after harvest. All measured parameters, except RAD, were significantly (p<0.05) affected by cultivar. Large differences observed between cultivars for all traits indicated genetic diversity among the studied potato cultivars. The highest and the lowest values of RL, RLD, RSA, R/S, RDW and PNA were found in Marfona and Nevita respectively. Based on cluster analysis, cultivars were divided into four different groups. Under the condition of this study, Marfona was superior whereas Arinda, Nevita, Marodana and Agria were inferior. Due to the high differences in root morphological characteristics and nitrogen uptake among potato cultivars, plant breeders may produce cultivars with larger root volumes and high nitrogen uptake.

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Azotobacter chroococcum is an important PGPR (Plant Growth Promoting Rhizobacteria) producing compounds needed for plant growth. The aim of this research was to study the effects of different native strains of Azotobacter chroococcum on growth and yield of wheat under greenhouse counditions. Seeds of spring wheat (Triticum aestivum L. var. Pishtaz) were inoculated with some Azotobacter chroococcum strains capable of producing IAA, HCN, sidrophore and fixing molecular nitrogen. The inoculation of wheat with those strains had a positive, significant effect on biological yield, seed protein percentage, thousand seed weight, leaf area, N, P, Fe and Zn uptake, in particular, by wheat. The increased growth of wheat was most likely due to the production of IAA and enhanced nitrogen fixation by inoculated strains. Some strains of Azotobacter chroococcum native to Chaharmahal va Bakhtiari are established as PGPR. Results also support the efficiency of Azotobacter chroococcum as an important biofertilizer in wheat cropping systems. The selected strains had a significant effect on wheat growth and yield, including biological yield and seed quality under greenhouse counditions. This beneficial effect of Azotobacter chroococcum on wheat is attributed mainly to IAA production and, to some extent to non symbiotic nitrogen fixation in the rhizosphere. So, these strains can potentially be used to improve wheat nutrition of micronutrients such as Fe and Zn, in particular.

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Sorghum (Sorghum bicolor L. Var. Speedfeed) is a major forage crop in Birjand Region, and phosphorus (P) plays an important role in its nutritional value. During a field campaign, eighty soil samples from the region were collected and analyzed. Among them, 24 samples varying in physico-chemical properties and available P were selected. Five extractants were used for measuring sorghum available P as follows: 1) 0.5 M NaHCO3, pH=8.5, shaken for 30 minutes (Olsen's method), 2) 0.5 M NaHCO3, pH=8.5, shaken for 16 hours (Colwel's method), 3) 0.0025 M Na2-EDTA, pH=7 (EDTA method), 4) 1 M NH4HCO3, 0.005 N DTPA (Soltanpour and Schwab's method), and 5) Water (Paauw's method). A greenhouse experiment using a completely randomized design was carried out with 24 soil samples, two treatments of 0 and 90 mg P/kg soil and three replicates. Results indicated that all five extractants are suitable for prediction of sorghum available phosphorus, but Olson and Paauw's methods are preferable. The results also show ed that the critical levels of soil phosphorus for sorghum by Olsen, Colwel, EDTA, Soltanpour and Schwab and Paauw methods are 17, 24, 14, 7 and 2.5 mg P/kg soil, respectively. Statistical analysis indicated that soil pH, clay percentage and organic carbon significantly affected sorghum available phosphorus.

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Cadmium is a toxic element in plant nutrition and is considered as a contaminant of food and feed. A greenhouse experiment was carried out to examine the effects of combined application of cadmium and zinc on responses of wheat on a clay loam soil texture. This experiment was designed in a factorial experiment with randomized complete block design (RCBD) and four replicates per treatment. Three levels of cadmium (Cd 0, Cd 50 and Cd 100 mg kg-1) and three levels of zinc (Zn 0, Zn 50 and Zn 100 mg kg-1) were added to the pot in factorial(9 treatments), and then Chamran seed variety of wheat (triticum aestivum L.) was planted in each pot. The statistical analyses showed that Cd application significantly (P0.01) decreased grain, straw, and total yield, but Zn application increased these parameters significantly (P0.01). Application of Cd separately decreased the grain yield by 61.5 while application of Zn separately increased the grain yield by 36.9. Cd application increased the Cd concentration and uptake by grain and straw as well as total uptake but by increasing Zn application, Cd uptake was decreased by wheat. Cd concentration was higher in straw than that grain, but Zn showed a different trend. Decreasing Zn applications decreased total uptake of Cd from 42.9 in Zn0 to 7.8 mg kg-1 in Zn100 treatment. Although this soil was very calcareous, this characteristic did not control Cd absorbtion.

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Different root water uptake models have recently been used. In this article, we use evapotranspiration data and soil water content data obtained from lysimeter measurements and root distribution in soil data obtained from olive tree to evaluate the accuracy of root water uptake models in predicting the soil water content profiles. Depth of lysimeter was 120 cm which was filled with clay-loam. Lysimeter recorded values of input and output of water and accurate value of evapotranspiration was also calculated. Soil water content distribution was measured using a TDR probe in lysimeter during the experiment. Feddes model with the root length density was used to account for the role of root distribution in soil. The flow equations were solved numerically with the measured evapotranspiration data as input, and the predicted soil water content profiles were compared with the measured profiles to evaluate the validity of the root water uptake models. The comparison showed that the average of relative error index for Feddes model was 10 %. Based on the results, about 90% of root uptake in olive tree happened at the depth of 40 centimeter

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Effects of five various tillage systems on soil porosity, volumetric water content, diurnal and nocturnal soil temperatures, plant water uptake and dry matter yield was investigated using a completely randomized design with five treatments and four replications in Gorgan University of Agricultural Sciences and Natural Resources research farm located in Seyed-Miran during 2009-2010 growing season. Tillage systems were: moldboard plough followed by one discing (20-25 cm) rototiller (12-17 cm) double disc (8-10 cm) Chisel plow (25-30 cm) No-tillage. Results show that during all stages of wheat growth, the highest and the lowest soil porosities at 0-8 cm depth were obtained by moldboard plough and No-tillage treatments respectively. For all stages except before tillering and harvest, the highest soil porosity obtained by moldboard treatment at 8-16 cm depth. Increasing tillage intensity increased those porosity that keep water in potentials greater than -5 and those in potentials less than -15 bar. No-tillage and moldboard induced the highest and the lowest soil temperatures respectively before earring stage. Tillage intensification, increased soil porosity and root density. More roots reduced soil water content in response to increased water uptake by wheat, resulting greater dry matter accumulation.

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Water Uptake by the root under salinity and water Stress in unsaturated soils was investigated through mathematical equations in three Groups of additive, multiplicative and non-consumptive. This study was an effort to assess six water uptake functions of van Genuchten (additive and multiplicative), Dirksen et al., Van Dam et al, Skaggs et al, and Homaee, for Rasht Hashemi rice under salinity and water stress conditions. Based on field observations of Hashemi Rasht rice in 1386 and 1389, crop growth simulation model of SWAP was calibrated and validated with a correlation coefficient of 0.97 and 0.95, respectively. Water Uptake Reduction Models' parameters were determined by the simulated data using SAS statistical software. Results showed that for the anticipated reduction of Water Uptake in rice water and salinity stress conditions for Rasht Hashemi rice, Homaee model is best.

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The pattern of root distribution in layered soils is one of the significant issues in the calculations of soil water and irrigation management and planning. The objective of this study was to determine the pattern of root distribution of soybean in layered soils and its effect on water uptake. The research was conducted in a completely randomized design with 15 treatments consisting of three different textures of soil (light, heavy, and medium) in four replications. The pattern of root distribution was monitored by the sampling of columns at the end of the growth period of the soybean. It was observed that the presence of the layer with medium texture has led to better plant development and growth after comparing the treatments in terms of plant growth. In general, root length density decreased with increasing soil depth, except in cases where there were different layers of soil, and root length density takes place in the following order: root length density in layers with medium texture≥ heavy texture≥ light texture. The rate of root water uptake rate was highest in the sandy layers, intermediate in clay, and lowest in loamy texture. Also, the rate of root water uptake rate increased significantly with increasing depth regardless of treatments. It can be concluded that the pattern of root distribution and plant growth is significantly affected by soil texture and its stratification.