JWSS - Journal of Water and Soil Science

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The ability of earthworms in recycling a wide range of organic solid wastes is well established. Only a few earthworm species are suitable for commercial vermicomposting. Two species, Eisenia foetida and Lumbricus rubellus, are common in temperate regions. Samples of earthworms were collected from manure pills and forest litter in North and Northwest of Iran. The samples were maintained in pots under greenhouse conditions. External morphological characteristics of mature worms were used in identifying earthworm species. These characteristics included: total number of body segments, numbers of clitellum and tubercula pubertatis (TP) segments, dorsal and external body color, body size, prestomium and prostomium shape, number of first segment with dorsal pore and patterns of clitellum and TP. All collected samples belonged to the species Eisenia foetida. Under incubation conditions in manure substrate (a moisture of 70% saturation at 24±2^oC), each worm produced 1-2 cocoons daily and each cocoon contained 2-7 worm embryos. The collected earthworm samples showed a good ability in vermicomposting of manure, plant residues, and some organic industrial refuses. The C:N ratio decreased during the vermicomposting process, which indicates the improved stage of decomposition and the vermicompost stability.

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  Understanding nitrogen mineralization from different organic sources should be a key factor in developing efficient prediction of the need for nitrogen fertilization with minimal negative environmental impact. In order to investigate nitrogen mineralization rate in soils amended with compost, vermicompost and cattle manure, an experiment was done as factorial in a randomized complete block design in three replications. Four treatments (compost, vermicompost, cattle manure and soil alone), two temperature levels (8 and 25 ⁰C) and two moisture levels (50% and 85% FC) were used for the 90 - day incubation study. Ammonium and nitrate were measured by spectrophotometer method. Results indicated that the mixed first-and zero-order kinetics model is the best model for our data. Cattle manure treatment had the highest N_min at 25 ⁰C (87.78 mgN/kg equal to 14.54% N_total) and the least value (23.62 mgN/kg equal to 4.62% N_total) was obtained for the compost treatment at 8 ⁰C. N₀k (nitrogen availability index) for treatments was in the following order: Cattle manure>Vermicompost >Compost. With increasing the temperature and moisture, N mineralization increased. Also N_min positively correlated with N₀ (r =0.583*), and N₀k (r =0.834**).

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In order to investigate the effect of vermicompost on physical and chemical properties of soil, an experiment was carried out in split plot based on complete randomized block design in three replications in Sari Agricultural Sciences and Natural Resources University. The physical and chemical properties of soil included bulk density, particle density, total porosity, water holding capacity, field capacity, permanent wilting point, available water capacity, pH, organic carbon and electrical conductivity in soil. Six levels of fertilizer treatments (T1= control, T2= chemical fertilizer, T3= 20 tons vermicompost + 1/2 T2, T4 = 20 tons / hac vermicompost + 1/2 T2 , T5= 40 tons vermicompost + 1/2 T2 and T6= 40 tons / hac vermicompost) and three levels of application years, one year of fertilization (1385), two consecutive years of fertilization (1385 and 1386) and three consecutive years of fertilization (1385, 1386, 1387). The results of the study showed that the application of these treatments in soil were significantly effective in increasing the total porosity, water holding capacity, field capacity, permanent wilting point, available water capacity, organic carbon electrical conductivity and in decreasing the bulk density, particle density and pH compared to control. In Contrast years of consumption of fertilizer did not have any significant effect on the physical properties of the soil except for FC, PWP, AWC, pH, OC and EC. The interaction between years of consumption of fertilizers were significantly different only in particle density and field capacity.

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Composting and vermicomposting are two efficient methods to recycle organic wastes. In order to investigate the structural changes in organic matter during the composting and vermicomposting processes, cattle manure and filter-cake of sugar cane were incubated with and without earthworm's presence in a controlled temperature and moisture condition for 16 weeks. Compound samples were taken at 0, 8, and 16 weeks. Carbon, nitrogen and C:N ratio were measured by a CN analyzer apparatus. Solid samples were used for structural analysis using FTIR and solid state CP MAS 13C NMR devices. C:N ratio was decreased with improving decomposition process. The C:N ratio was slightly elevated after 16 weeks of vermicomposting. Structural analysis with both spectroscopic methods showed a decrease of O-alkyl C and increase of aromatic and carboxylic functional groups. The analysis of samples with 13C NMR showed that after 16 weeks, the percentage composition of O-alkyl filter-cake decreased from 41 percent in the first sample to about 33.1 percent. The amount of these compounds in manure samples decreased from 56.7 percent to 43.6 percent. Aromatic compounds in samples of raw filter-cake and manure increased from 12.5 and 13.5 percent to 16.4 and 18.7 percent, respectively. Percentage of carboxyl compounds increased, respectively, from 8.5 and 5.6 percent to 9.7 and 7.2% in the filter-cake and manure sample. Vermicomposts had higher content of aromatic groups compared to composts (In manure samples 18.7 instead of 17.1 percent). After 16 weeks of incubation the aromatic and carboxylic compounds slightly decreased in the vermicomposting process. Analysis of composts and vermicomposts with FTIR and 13C NMR resulted in similar signals with the different frequency intensities. There was a suitable correlation (r=0.897**) among the spectroscopic methods in characterization of composts and vermicomposts

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Improper properties of sewage sludge include odors, trace elements concentration, and presence of pathogens limit its use in agriculture. Composting and using of earthworms in this process is one of the most suitable methods for stabilization and removal of unsuitable properties of sewage sludge. To investigate the effects of kind and mixing proportion of bulking material and sewage sludge decomposition stage on vermicompost quality, an experiment was carried out in a split plot design with three replications. In this experiment, the stage of sewage decomposition (raw sewage, digested sludge in digester, dewatered sludge in lagoons, and final dried sludge in lagoons) was main factor and kind of bulking materials (woodchips, three leaves, and wheat straw) and mixing proportion of bulking materials to swage sludge (0, 15, 30, and 45 %, V/V) were as subordinate factors. Chemical properties of sewage in different decomposition stages and bulking materials were determined. After 5 months of incubation in the controlled moisture and temperature condition, and in the presence of earthworms, the pot's contents were harvested, and nutrients and heavy metal concentration of vermicomposts were measured. Vermicomposts had relatively low pH and EC. Their nutrients content especially N, P, Fe, and Zn were high. These properties showed vermicompost suitability for use as a soil amendment and organic fertilizer. Heavy metals concentrations of vermicomposts, except for Zn, were lower than recommended values by EPA. Despite no significant difference between the kinds of bulking materials, mixing of these materials with sewage sludge decreased pH, N, and Pb content of produced vermicompost. According to the results, 30% (v/v) mixing of bulking material and sewage sludge is recommended

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A study was conducted to evaluate the effect of vermicompost and nitrogen (N) on growth, nutrient concentration and uptake of N, phosphorus (P), potassium (K), iron (Fe), zinc (Zn), manganese (Mn), and copper (Cu) in sunflower shoots as a factorial experiment arranged in completely randomized design (CRD) with three replications under greenhouse conditions. Treatments included three levels of vermicompost (0, 2.5, and 5 % by w/w) and three N levels (0, 90, and 180 mg kg-1) as urea. Application of 2.5% vermicompost significantly increased the average fresh and dry weights, and uptake of K, Fe, Zn, Mn, and Cu of shoots as compared when no vermicompost was applied, but decreased Zn and Cu concentrations. Application of 90 mg N, increased fresh and dry weights, and concentration of N, Zn, and Mn, total uptake of N, P, K, Fe, Zn, Mn, and Cu in sunflower shoots, but decreased P concentration. Application of 180 mg N increased total N uptake than that of 90 mg N kg-1 treatment. Combination of 2.5% vermicompost with 90 mg N, significantly (p<0.05) increased dry matter yield of sunflower as compared to 2.5% vermicompost without N treatment

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Although addition of amendment materials to soil is not for changing of soil K status, secondary effects of these materials can be important in K distribution among soluble, exchangeable and non-exchangeable forms. In this research, effect of addition of 2% zeolite, 2% vermicompost and 1% zeolite+ 1% vermicompost on 10 calcareous soils of Fars province belonging to five orders was investigated. The experiments were done as a randomized complete block with three replicates. Samples were incubated at 22±2 °C and 50% of saturated moisture content for 90 days. Then different forms of K were determined in the samples. Zeolite application induced 279 mg kg-1 increase in exchangeable K and 24 mg kg-1 decrease in non-exchangeable K, but it had not a significant effect on soluble K. Although vermicompost application increased soluble and exchangeable K, its effect was more sensible for soluble K (139 mg kg-1). Zeolite+vermicompost application increased all forms of K. Increase in soluble K with vermicompost application may increase risk of K leaching but zeolite application can preserve K in exchangeable phase and inhibit K leaching and fixation.

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Current study conducted to evaluate the effects of vermicompost and zeolite on the kinetics of nickel (Ni) fixation. Treatments consisted of a factorial combination of two vermicompost levels (zero and 2 w/w percent), three zeolite levels (zero and 4 w/w percent of zeolite of Firoozkoh, and Semnan) and two soil textures (clay and sandy loam) in three replications. All treatments were spiked with 50 or 100 mg Ni kg-1. DTPA extractable Ni was determined after 5, 10, 20, 30, 60 and 90 days. Ni availability was higher in sandy loam texture. Vermicompost application increased Ni availability in sandy loam texture in all the designated times. Zeolite application had no significant effect on Ni availability. The trend of Ni availability decrease was composed of two distinct stages with high and low Ni fixation rates. In the first step which continued up to 30 d, the available Ni fixation rate was high and then decreased sharply. Ni fixation data was suitably prescribed using simple Elovich and exponential equations. It seems that vermicompost has a greater effect to prevents Ni fixation and to retain it in available form in light texture soils. On the other hand, it seems that zeolite does not have any considerable effect on Ni fixation in calcareous soils.

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