A. Zeynaddini, B. Jafarpour, M. Flahaty Rastegar,
Volume 9, Issue 2 (7-2005)
Abstract
The study of dispersion of alfalfa mosaic virus (ALMV) infection based on DAS-ELISA indicated that the fields of Alfalfa, potatoes and tomatoes from Chenaran, Ghochan, Shirvan, Mashhad, Neishaboor and Torbat Heydarieh were infected with the virus. The Statistical analysis indicated that the amount of infection did not differ in the surveyed regions and total mean of infection was 53 percent. The samples collected from the Alfalfa field of Mashhad was propagated in the Nicotiana tabacum L.cv Samsun and then virus was purified. The Mechanical inoculation of this isolate of the alfalfa mosaic virus (ALMV) induced the local lesion in Chenopodium quinoa,C. amaranticolor,Vigna unguiculata, Phaseolus vulgaris cv Redkidney and the systemic vein clearing and mosaic in Nicotiana glutinosa, N. tabacum cv. Samsun, Ocimum basilicum, Cicer arietinum and Lycopersicon esculentum. In the case of the infected Cucumis sativus, no symptoms was observed. ALMV was purified by the method of the kaiser and Robertson(1976). The virus yield was 11/05 mg per 100g of infected tissue on the basis of serological properties. This isolate of ALMV is similar to the American isolation in SDS-PAGE and Western Blot analysis,the molecular weight of the virus coat proteins were estimated at about 24000 daltons.in this regard,this isolation of ALMV is similar to the other isolates of ALMV reported elswhere.
M. Shabanian, H. Masomi, A. Hoseinipour, J. Heidarnejad, Z. Azami,
Volume 11, Issue 1 (4-2007)
Abstract
Cucumber cultivars, grown in greenhouse in the Jiroft region, were surveyed for the relative incidence of Zucchini yellow mosaic virus (ZYMV), Watermelon mosaic virus-2 (WMV-2), Cucumber mosaic virus (CMV) and Tomato spotted wilt virus (TSWV) from 2001 to 2004. Samples from 1294 plants representing different cultivars were analysed by Enzyme-linked immunosorbent assay (ELISA) and Dot immunobinding assay (DIBA). The data showed that green-house cucumbers are infected by CMV, ZYMV, TSWV and WMV-2. However, ZYMV was the most prevalent virus. Mixed infection including double and triple infection was identified in some samples. Transmission of aphid-borne viruses (CMV, ZYMV and WMV-2) by Aphis gossypii, A. fabae, A. craccivora and Myzus persicae revealed that ZYMV is most efficiently vectored by these aphids and, A. craccivora transmitted these viruses with more than 60% efficiency. In addition, WMV-2 was not transmitted by A. gossypii. In RT-PCR, ZYMV infection was confirmed by amplifying a PCR product of the predicated size 458 bp, using total RNA extracted from infected plants. All ZYMV infected samples reacted with monoclonal antibodies (705-1, 705-2 and 705-4) in TAS-ELISA test. These results showed that ZYMV isolate collected from Jiroft belongs to group A, cluster 1 or 2. In electron microscopy study, normal length of ZYMV flexuous particles in partial purified preparation was calculated as 790 nm. The molecular weight of coat protein of ZYMV was estimated at 36 KDa., using SDS-PAGE and western blotting. This is the first report of these viruses in greenhouse grown cucumber in the Jiroft region.
