JWSS - Journal of Water and Soil Science

---

Micronutrients availability including that of Zn is critical for optimum growth of plants. Zinc availability in calcareous soils of Iran is relatively low due to the presence of a large amount of CaCO₃ and high pH levels. Overapplication of phosphorus to soils may also cause P-induced Zn deficiency. The objective of this experiment was to evaluate the effect of phosphorus and zinc application on the growth and chemical composition of corn (Zea mays L.) under greenhouse conditions. Treatments consisted of a factorial combination of 5 levels of P (0, 25, 50, 100 and 200 µg P/g soil as KH₂PO₄) and 3 levels of Zn (0, 5 and 10 µg Zn/g soil as ZnSO₄. 7H₂O) in a completely randomized design with 3 replications. Plants were allowed to grow for 60 days and then cut at the soil surface.

 Results showed that P and Zn applications increased top dry weight. Applied P increased P concentration and total uptake in plants, but decreased Zn concentration and had no effect on Zn uptake. Zinc application decreased P concentration of com but increased Zn uptake and concentration. The P: Zn ratio in plants increased with P application but decreased with Zn addition. Application of P and Zn increased Fe concentration in plant but decreased Mn concentration and had no effect on Cu concentration. Prior to making any fertilizer recommendations, more research is required to precisely evaluate the response of com to P and Zn applications under field conditions.

---

A factorial experiment with two levels of Zn (0 and 1.5 mg Zn kg^-1), five salinity levels of irrigation water (0, 60, 120 and 180 mM NaC1, and 120 mM NaNO₃) in three replications was conducted. Wheat (Triticum aestivum cv. Roshan) was seeded in pots. After plant harvesting, zinc and cadmium concentrations were determined in the shoot. Activities of metal species in the soil solution were predicted using the computer program MINTEQA2. Treating the soil with NaC1-salinized water increased total concentration of cadmium (Cd_T) as well as Cd²⁺, CdC1⁰₂, and CdCl⁺ species whereas, NaNO₃ treatment had no significant effect on Cd_T. Shoot Cd concentrations were positively related to Cd_T and soil solution Cl^- but negatively related to Zn_T. Application of Zn-fertilizer decreased Cd and increased Zn concentrations in shoot, significantly. The results of this experiment showed that Cl^- has an effective role in increasing mobility of soil Cd and its uptake by plant.

---

---

In order to investigate the effect of zinc - chelate on drought tolerance of Azadi cross wheat, a randomized complete block design was conducted as split plot with three replicates in the Kherameh during 1383 - 1384. The main plot was four drought levels (control and drought stress in the stages of flowering, seed milk stage and two phases, together), and sub plot was zinc - chelate rates 0, 5, 10 & 15 kg per hectare. The results showed that zinc application under drought conditions increased spike per square meter significantly at the 5% level. Drought stress decreased 1000 - seed weight. Least of 1000 - grain weight was in two phases of flowering and seed milk stage together (29.78 g). The application of 15kg zinc -chelate fixed 1000 - seed weight. Treatments of drought stress decreased seed yield significantly (14.17% in the proportion of control), but zinc - chelate application increased wheat tolerance to seed yield decrease. Zinc - chelate application prevented from seed number decrease per wheat spike under drought conditions that was caused to tolerance of seed yield and harvest index decrease. The application of 15 kg zinc - chelate increased harvest index in comparison of control amount of 22%.

---

Sewage sludge application on farmland as fertilizer is commonly practiced in many countries. Sewage sludge is rich in macro and micronutrients. However, high concentration of heavy metals in sludge may cause pollution of soil, groundwater and human food chain because of toxic metals uptake by crops. The objective of this study was to determine residual and cumulative effects of sewage sludge on concentration of Zn and Cu in soil and wheat. Different levels of 0, 25, 50 and 100 Mg ha-1 of sewage sludge were applied to the soil for four years. To study the cumulative and residual effects of the sewage sludge, applications were repeated on three fourth of each plot in the second year, on one half of plots in the third year and on one fourth of plots in the fourth year. Wheat was grown in the plots. After the fourth year, soil samples from the 0-20 cm depth of the different parts of the plots were taken and analyzed. After harvesting the wheat, roots, stems and grains were separately analyzed for the heavy metal concentrations. Cumulative sewage sludge application significantly (P≤0.05) increased the total and DTPA-extractable concentration of Zn and Cu in soil. Residual sewage sludge in the soil also increased the total and DTPA-extractable concentration of Zn and Cu. Single sludge applications at different rates increased the DTPA-extractable concentrations of heavy metals. In subsequent years with no further sludge application, DTPA–extractable metal concentrations in soil decreased continuously, approaching the levels in the control. However, even after four years, DTPA-extractable concentration of Zn in plots receiving more than 50 Mg ha-1 and Cu in plots receiving more than 25 Mg ha-1 sludge, were still significantly higher than control. DTPA-extractable concentrations of metals were closely correlated with total concentrations. Sewage sludge had a significant effect on concentration of Zn and Cu in stems and grains. Cumulative effects on Zn and Cu uptake by stems were more than residual effects. The results of this study show that cumulative and residual effects of sewage sludge application increased concentrations of micronutrients in soil and wheat.

---

The objective of this study was to evaluate the possibility of using treated industrial by-products as a zinc (Zn) source and compare their Zn availability and efficiency to ZnSO4. A greenhouse factorial experiment in a completely randomized block design was conducted with corn (Zea mays L.) in triplicates. Treatments included three Zn sources (ZnSO4, IUT-UT in size of < 1 mm and IUT-UT in size of 2-3 mm) at three rates (0, 20 and 40 kg ha-1). The results showed that Zn application both in the form of ZnSO4 and treated industrial by-products significantly (P < 0.05) increased the growth and shoot dry matter yield of corn. Shoot dry matter weight of corn in pots that had received IUTUT was higher compared to those that had received ZnSO4. The highest shoot dry matter weight was produced at the IUT-UT treatment in size of < 1 mm. Increased fertilizer rate significantly (P < 0.05) enhanced shoot and root Zn concentration. However, this increase was higher in ZnSO4 treatment as compared to the IUT-UT treatments. Application of the IUT-UT caused a significant increase in shoot Fe concentration of corn. Shoot and root Cd concentration in all experimental treatments was less than 0.02 mg kg-1. The results showed that IUT-UT can be used as a slow-release Zn fertilizer with low impurity.

---

This study was carried out to evaluate the application of zinc and copper sulfate by different methods such as foliar application and deep-placement and time of application on the some quantitative and qualitative properties of pistachio trees. For this purpose, a complete randomized block design experiment with 12 fertilizer treatments and 3 replications at 2 consecutive years was conducted in the experimental garden of Rafsanjan. Treatments included deep-placement of medium amounts (54.2 and 11.1 kg/ha of elemental Zn and of Cu respectively) and high amounts (81.3 and 16.7 kg/ha of Zn and of Cu respectively), foliar application of 0.15 kg/ha elemental Cu in the form of Cu sulfate and 1.8 kg/ha of the elemental Zn in the form of Zn sulfate (34%) at the rate of 1000 liter water on Esfand (late dormant), Farvardin (after flowering) and Mehr (post harvest) . Results indicated that in first year, the maximum yield was obtained with foliar application of Cu on Esfand. Also, qualitative properties such as Split/non split ratio significantly increased by above treatment. The protein content increased with foliar application of Cu on Esfand and Zn on Farvardin by 22 and 16.5%, respectively compared to the control. The analysis of leaf samples, demonstrated that the foliar spray of Cu+Zn on Esfand increased Zn leaf by 119% as compared with control. In second year, yield increased by the foliar application of Zn on Esfand. Also, qualitative properties such as the number of seeds per ounce significantly increased by foliar application of Cu on Mehr. The highest and the lowest rate of split/non-split ratios were observed in treatments of high and low localized placement. The protein content decreased in the treatment of high deep placement as compared to the control. Foliar spray of Zn on Esfand caused the highest increase of leaf Zn concentration and foliar spray of Cu and Zn+Cu in Mehr increased significantly Cu leaf. The results indicated that the effects of treatments on oil content and stem nutrients concentration were not significant on two years. Due to saline and lime effects in the soil in pistachio orchards, soil application of micronutrients had no significant on the the yield.

---

Due to low availability of zinc and copper in calcareous soils and the antagonism of these nutrients with each other, the study of their effects on growth and chemical composition of rice seems to have great importance. Also, the relations among different chemical forms of Zn and Cu and plant responses are very important, which can be used in investigation of antagonisms of these nutrients as well. Therefore, by application of different levels of Zn and Cu, their effects on plant growth and nutrient concentration in the plant were studied and different forms of these nutrients in soil were determined. Results showed that zinc application increased plant growth, Zn concentration and total uptake, but decreased Cu concentration in the plant. However, copper application showed no significant effects on the plant growth, but increased Cu concentration and total uptake and decreased Zn concentration in the plant. Also, the results showed an increase in carbonatic, adsorbed and exchangeable Zn forms and exchangeable Cu form by Zn application. Carbonatic form of zinc has maximum effects on concentration and total uptake of this element in the plant. Copper application increased carbonatic and organic forms of this nutrient in soil, and there was maximum correlation between organic form of Cu and its concentration and uptake in the plant

---

A greenhouse experiment was conducted in factorial experiment arranged as a completely randomized design (CRD) to evaluate the role of tree indigenous arbuscular mycorrhizal fungi (AMF) species originated from a polluted soil in phytoremediation of zinc polluted soils using maize as a host plant. The experiment consisted of plants inoculated with AMF (G1(Glomus intraradices), G2(Glomus mosseae) and G3(Glomus versiforme)) and G0 as non-inoculated plants and 5 levels of zinc (0, 10, 50, 100 and 500 mg kg -1) in non-sterilized sandy loam soil with three replications. According to the results of greenhouse experiment, the zinc and phosphorus uptake and also the biological yield of maize plants were significantly increased by inoculation with AMF in comparison with non-inoculated plants and also no zinc toxicity symptoms were observed. Uptake, translocation, and phytoextraction efficiency of plants inoculated with G. intraradices was more than the other treatments up to the level of 100 mg kg -1, but at the level of 50 mg kg -1 these amounts were highest in plants inoculated with G. mosseae. The efficicncy of three AMF in zinc uptake was highest at the low level of zinc. In general, under the high soil pollution (500 mg kg-1), G. mosseae was the most effective fungal species in Zn extraction and translocation while G. intraradices had the highest effectiveness for accumulation of Zn in the roots. The overall situation of G. versiforme was mostly between the two other fungal species.

---

Micronutrients such as Fe and Zn in adequate level are essential for plant growth cycle and impose a vital role in increasing yields of most agricultural crops. Using organic wastes such as sewage sludge, compost and manure is a proper practice for returning organic matter and some nutrients into the soil, particularly in arid and semi-arid regions. The objective of this study was to determine the effects of sewage sludge, compost and cow manure on availability of Fe and Zn in soil and their uptakes by corn, alfalfa and targetes flower. A completely randomized design with three treatments (sewage sludge, compost and cow manure at 25 Mg/ha) was used. Application of sewage sludge, compost and cow manure significantly increased availabile Fe and Zn of the soil. The application of organic wastes increased the dry matter yield of the plants. Fe and Zn concentration of plants shoots in organic amendments treatments were significantly greater than blank. The highest mean concentration of Fe in plant tissues was obtained in the cow manure and the highest mean concentration of Zn in plant tissues was obtained in the compost treatment.

---

The growth and fluorescence parameters of chlorophyll in four corn hybrids including two sweet (K.S.C. 403 and K.S.C. 404) and two grain hybrids (S.C. 500 and S.C. 700) were evaluated in response to Fe and Zn nutrition in a nutrient solution culture. This study was conducted in a randomized complete block design with a factorial arrangement with three replications at the Soilless Culture Research Center (SCRC). Corn hybrids were exposed to two Fe levels (5 and 50 µM Fe in the form of FeEDTA) and two Zn levels (0 and 2 µM Zn in the form of ZnSO4). Increasing Fe concentration in the nutrient solution increased F0, Fm, and Fv/Fm values by 48, 96, and 123%, respectively. The fluorescence parameters of chlorophyll were affected by corn hybrid and there were significant differences in F0 and Fv/Fm at 1% level and Fm at 5% probability level among the studied hybrids. Iron deficiency significantly (P<0.05) reduced the root dry weight of all the studied hybrids. Zinc deficiency resulted in a significant decrease in the root dry weight of H403 and H500 hybrids while no significant decrease was found in the root dry weight of the H404 and H700 hybrids under Zn condition. The results showed that corn hybrids varied significantly in their tolerance to Fe and Zn deficiency. Based on the results of plant growth and fluorescence parameters of chlorophyll, the studied grain corn hybrids were more tolerant to Fe and Zn deficiency conditions in comparison with sweet corn hybrids. It seems fluorescence parameters of chlorophyll might be suitable parameters to screen corn hybrids in their tolerance to Zn and particularly Fe deficiency condition.

---

Waste tire rubbers are considered one of the environment pollutants. Increased production of these pollutants has led to more serious consideration of ways to reduce the harms caused by their accumulation in the environment. Therefore, the effects of incorporation of waste rubber crushed particles in two sizes of 1-2 and 3-5 mm and the amounts of 0, 5, 10 and 20 Mg ha-1 in a calcareous soil (0-30 cm depth) on some chemical properties of soil was investigated in Isfahan University of Technology research field (Lavark). This experiment was performed using seven treatments along with a non amended control treatment in a randomized complete block design with three replications in 1387. Eight months after incorporation, soil samples were collected for laboratory analyses. Results of ANOVA tables show that soil pH, electrical conductivity, percentage of total nitrogen and DTPA-extractable concentrations of cadmium, lead, copper and iron were not significantly affected by application of rubber particles. Incorporation of rubber particles into the soil significantly increased soil organic carbon and carbon to nitrogen. Increases in the DTPA-extractable Zn in soils treated with 10 and 20 Mg ha rubber particles in fine and coarse sizes were significant in comparison with the control soil. DTPA-extractable Zn content in the soil treated with 10 and 20 Mg ha 3-5 mm waste tire rubbers particles was about two and three times higher than that in the control treatment, respectively. The results of this study showed that in short-time, incorporation of crushed tire rubbers particles had no significant effect on most chemical properties of the soil but increased the available Zn content. In this regard, further studies to monitor the effects of adding waste rubber crushed particles on organic matter mineralization, plant toxicity and physical properties of soil in long-term are recommended.

---

This study was carried out to investigate zinc (Zn) and cadmium (Cd) concentration in paddy soil and rice grain produced in central and southwest Iran in relation to soil and plant factors, and their intake in peoples diet was also assessed. Rice crops and associated surface soils (0-30 cm) were collected from 136 fields in Isfahan, Fars and Khuzestan provinces. The result, showed the DTPA-Zn concentration in more than 50% of paddy soils was less than its critical deficiency concentration (2 mg kg-1). The grain Zn concentration in more than 54% of the rice samples was less than 20 mg kg-1. The measured Cd concentrations in paddy soils and edible part of rice exceeded the world health organization (WHO) value in more than 12% of the samples. By considering the average daily rice consumption of 110 g per capita, the Zn intake from rice consumption was estimated about 10% needed for female and male adult. Diet intake analysis did not indicate any excessive dietary intake of Cd when Cd mean of concentrations in rice grain was 0.04 mg/kg, but based on the results of risk analysis, it is more than safely level for contaminated rice.

---

Cadmium is a toxic element in plant nutrition and is considered as a contaminant of food and feed. A greenhouse experiment was carried out to examine the effects of combined application of cadmium and zinc on responses of wheat on a clay loam soil texture. This experiment was designed in a factorial experiment with randomized complete block design (RCBD) and four replicates per treatment. Three levels of cadmium (Cd 0, Cd 50 and Cd 100 mg kg-1) and three levels of zinc (Zn 0, Zn 50 and Zn 100 mg kg-1) were added to the pot in factorial(9 treatments), and then Chamran seed variety of wheat (triticum aestivum L.) was planted in each pot. The statistical analyses showed that Cd application significantly (P0.01) decreased grain, straw, and total yield, but Zn application increased these parameters significantly (P0.01). Application of Cd separately decreased the grain yield by 61.5 while application of Zn separately increased the grain yield by 36.9. Cd application increased the Cd concentration and uptake by grain and straw as well as total uptake but by increasing Zn application, Cd uptake was decreased by wheat. Cd concentration was higher in straw than that grain, but Zn showed a different trend. Decreasing Zn applications decreased total uptake of Cd from 42.9 in Zn0 to 7.8 mg kg-1 in Zn100 treatment. Although this soil was very calcareous, this characteristic did not control Cd absorbtion.

---

Mycorrhizal fungus colonization of roots may modify plant metal acquisition. In order to study the role of external hyphae of mycorrhizal fungi in metals transferring, the root and hyphal growth zone were separated by 30 µm nylon meshes. Plants were inoculated with G. mosseae and G. intraradices and grown in the root compartment. Six combinations of metals (400 mg of Zn, 25 mg of Cd, 400 mg of Zn + 25 mg of Cd, 50 mg P, 50 mg of P+ 400 mg of Zn + 25 mg of Cd, and no metal kg-1 soil sample) were added to the hyphal compartments. This experiment was carried out as a completely randomized design (CRD) with factorial arrangement in three replications. The results showed that dry shoot weight and phosphorus concentration in mycorrhizal treatments increased significantly compared with non mycorrhizal treatments. of the two fungal species, G. mosseae had more effect on phosphorus concentration in white clover plant. AM fungi increased the Zn concentration in shoot and root of white clover. AM fungi also significantly increased cadmium concentration in root of mycorrhizal treatments compared to non mycorrhizal plants. of the two fungal species, G. intraradices accumulated more cadmium in clover roots. ALL in all the results demonstrated that AM fungi increased phosphorus and Zn transferring to the shoots while cadmium transferring was decreased by stabilizing in the roots.

---

Physicochemical characteristics of soil, land cover/use and human activities have effects on heavy metals distribution. In this study, we applied Classification and Regression Tree model (CART) to predict the spatial distribution of zinc in surface soil of Hamadan province under Geographic Information System environment. Two approaches were used to build the model. In the first approach, 10% of total data were randomly selected as test data and residual data were used for building model. In the second approach, all data were used to build and evaluate the CART model. Determination coefficient (R2) and Mean Square Error (MSE) were applied to estimate the accuracy of model. Final model included 51 nodes and 26 terminal nodes (leaf). Calcium carbonate, slope, sand, silt and land use/cover were determined by the CART model to predict spatial distribution of Zn as the most important independent variables. The regions of western Hamadan province had the highest concentration of Zn whereas the lowest concentration of Zn occurred in the regions of northern Hamadan province. The results indicate good accuracy of CART model using R2 and MSE indices.

---

This research was carried out in a hydroponic culture to investigate the effect of Zn nutrition on phytosiderophore release by roots of three bread wheat genotypes (Triticum aestivum L. cvs. Rushan, Kavir, and Cross) differing in Zn-efficiency. The wheat seeds were germinated in sterile sand and two weeks later the plants were transferred to nutrient solution containing different Zn levels. Phytosiderophore released by plant roots was collected ten days after applying Zn treatments and measured using resin-Cu-mobilization test. A month after their transfer to nutrient solution, the plants were harvested and Fe and Zn concentrations in root and shoot were measured, and total amounts (uptake) of these nutrients were determined. Zinc addition increased concentration and total amount of Fe and Zn in shoot in Rushan genotype, while it had no significant effect on concentration and total amount of Zn in shoot and root of Kavir and Spring Back-Cross-Rushan genotypes. Addition of Zn to the nutrient solution decreased concentration and total amount of Fe in shoot of all wheat genotypes. On the other hand, Zn nutrition increased root Zn concentration in Rushan and Kavir genotypes, while it resulted in significant decrease of root Zn concentration in Back-Cross-Rushan genotype. Effect of Zn nutrition on the amount of phytosiderophore release by roots of wheat genotypes was different. Zinc nutrition resulted in an increase of phytosiderophore release by roots of Rushan, while it had no significant effect on phytosiderophore release in other wheat genotypes.

---

Studying the distribution of Zn in the soils allows investigating their mobility and bioavailability. In this research, 10 calcareous soil samples were selected, and sewage sludge-amended (1% w/w) and -unamended (control) soils were incubated for 1 month. Before planting, fractions of Zn were determined, three seeds of wheat were planted in each pot and after 8 weeks, they were harvested. Results showed that all Zn fractions were increased by sewage sludge addition. Results of pot experiment indicated a significant correlation between Zn concentration in wheat with exchangeable (r=0.92**) and Zn associated with Fe and Mn oxides (r=0.76*) in control soils. In addition, significant correlation coefficient was found between Zn associated with Fe and Mn oxides and Zn uptake index (r=0.86**). Moreover, wheat responses were significantly correlated with Zn exchangeable (r=0.71*- 0.84**) in amended soils. Yield was correlated with Zn associated with carbonates and Zn associated with Fe and Mn 0.69 and 0.75, respectively. Zn uptake index was correlated with Zn associated carbonates (r=0.64*). Therefore, the exchangeable and Zn associated with Fe and Mn oxides in control soils and the exchangeable and Zn associated carbonates and Zn associated with Fe and Mn oxides in amended soils could be used as available fractions of wheat.

---

Heavy metals contamination of soil and plants has very important and vital role in relation to health and life of human and other organisms. The aim of this study was to assess the efficiency of sorghum (Sorghum bicolor) and sommon lambsquarter (Chenopodium album) in phytoremediation of Zn from soil. Efficiency of 0.01M CaCl2, 0.1M NaNO3, and 1M NH4NO3 for extraction of “bioavailable fraction of soil Zn was also compared. Correlation between the Zn concentrations extracted by these methods and plants response (relative yield and shoot Zn concentration) to soil Zn contamination was then evaluated. For this purpose, a calcareous soil sample was contaminated with different concentrations of Zn. Sorghum and common lambsquarter, were grown in pots containing the contaminated soil and were analysed for their Zn concentrations after harvest. Results of this study showed that, common lambsquarter was more tolerant to low and medium concentrations (≤900 mg/kg), but sorghum tolerated high concentration of Zn. Also sorghum was more capable in removal of Zn from soil in comparison to common lambsquarter, so that soils contaminated with low Zn levels (≤900 mg/kg) can be remediated by sorghum. In addition, there was a significant correlation between 1 M NH4NO3-extractable soil Zn and the plants response (relative yield and shoot Zn concentration) to soil Zn contamination.

---

Rhizosphere is a small zone and has quite different chemical, physical, and biological properties from bulk soil. This research was performed to study the available Zn and its fractions in the wheat rhizosphere and bulk soils by using rhizobox at greenhouse conditions. Total organic carbon (TOC), dissolved organic carbon (DOC), microbial biomass carbon (MBC) and available Zn using 7 chemical procedures and Zn-fractions were determined in the rhizosphere and bulk soils. The results indicated that TOC, DOC and MBC in the rhizosphere were increased significantly (P<0.01). Zn extracted using several chemical extractants (except 0.01 M CaCl2) in the rhizosphere were significantly (p<0.05) higher than in the bulk soils. Also, concentrations of Zn-fractions (except carbonate-associated) in the rhizosphere were significantly (p<0.05) different from concentrations of Zn-fractions in the bulk soils. Results indicated that correlation between uptake index and extracted Zn using DTPA-TEA and Mehlich 3 were significant (p<0.05). Moreover, significant correlation (p<0.05) between uptake index and exchangeable Zn and Zn associated with iron-manganese in the wheat rhizosphere and bulk soils were found. The results of this research illustrated that available Zn and its fractions in the rhizosphere soils are different from the bulk soils. Therefore, application of the rhizosphere soil would be recommended in the study on these properties after planting.