Showing 5 results for Pathogenicity
M. Niknejad Kazempour,
Volume 6, Issue 1 (4-2002)
Abstract
Pseudomonas syringae is a phytopathogenic bacterium with a wide host range. The biology of this bacterium consists of two phases. The first phase is the indication of disease on the host plant which generally appears in the form of necrosis on the aerial parts of plant (pathogenicity phase). The second phase is a rapid multiplication of bacteria on the aerial surface of the plant without inflicting any defense response (epiphytic phase). In this study, the impact of pathogenic virulence genes of aggress, dsp, ice, cor and hrp genes in the epiphytic process of Pseudomonas syringae on resistant and sensitive varieties of tomato is examined. The population dynamism of bacterial colonization on the root, stem and leaves was studied.
The results indicated that the hrp genes system (hypersensitive reaction and pathogenicity) in Pseudomonas syringae pv. tomato, P. s. pv. syringae and P. s. pv. phaseolicola and the coronatine gene system in P. s. pv. tomato are necessary for colonization on both host and non-host plants. The mutants isolates of hrp and cor compared with wild isolates could colonize at a lower level on all parts of the plant. In contrast, the ice (Ice nucleation), aggress (Agressivity) and dsp (disease specific) gene systems had no significant impact on the epiphytic colonization of P. s. pv. syringae. However, under in vitro conditions no significant difference was observed among the wild type isolates and their mutants in king B medium in the bioscreen machine. It is concluded that the lower multiplication of P. syringae mutants in planta is due to the plant-bacteria interaction.
S.a. Safavi, Gh.r Rassulian Etal,
Volume 6, Issue 1 (4-2002)
Abstract
Pathogenicity and virulence of entomogenous fungus, Verticillium lecanii, was studied on the pea aphid, Acyrthosiphon pisum. Vertalec, a commercial product of V. lecanii, was evaluated under controlled conditions. Second instar nymphs were inoculated with conidial suspensions at concentrations ranging from 104 to 108 conidia/ml, and sterile distilled water (containing wetting agent) as control, then caged on cutted alfalfa stems. Each concentration was replicated three times with 30 aphids treated per replicate. Temperature, relative humidity and photoperiod were maintained at 23±1 °C, %97±3, and 16:8 (L:D), respectively. Aphids were monitored daily over 12 days for mortality and nymph production. All newborn nymphs and dead aphids were removed daily.
Vertalec significantly increased aphid mortality (mean mortality because of mycosis increased form %45.55±6.93 at 104 conidia/ml to %95.55±4.45 at 108 conidia/ml). The LC50 value for pathogen was 5.l4×l04 conidia/ml. LT50 values for 105, 106, 107 and 108 conidia/ml were 10, 8, 6.5 and 5 days, respectively. At 104 conidia/ml LT50 value was not determined at experimental period. Ro values significantly decreased with increasing conidial concentration (mean R0 altered from 28.l5±5.38 in control to 5.15±1.81 at 108 conidia/ml). Results indicated that vertalec can be an effective agent against pea aphids. Further studies are recommended for its evaluation under natural conditions.
M. Motallebi, M. R. Zamani, A. Hosseinzadeh-Kalagar,
Volume 6, Issue 4 (1-2003)
Abstract
A total of forty-three isolates of Ascochyta rabiei obtained from infected seeds and plants of chickpea from different areas of Kermanshah, Lorestan, Hamadan, Kordestan, and East and West Azarbaijan provinces were studied during the period 1996-1999. A rapid and simple method was developed for pathogenicity test in CDA (chickpea seed meal dextrose agar) medium. CDA medium was inoculated with different isolates and incubated at 21±1 oC for 9 days. The disease severity was scored as 0-100% at 24-hour intervals up to five days. Virulence forms were designated according to spectrum of disease reactions of each isolate. Thirteen isolates (IK04, IK08, IK10, IK13, IK14, IK17, IK19, IK21, IL01, IL10, IO00, IE04, IE06) were distinguished as highly virulent (HV) and 7 isolates (IK03, IK06, IK15, IE01, IE08, IL04, IL06) as weakly virulent (WV). Polygalacturonase activity of these isolates from 6 days old inoculated PZ (pectin zymogram) medium were assayed. Comparison of the results of pathogenicity test and PG activity of 43 isolates demonstrated that HV isolates show predominant enzyme activity while the WV isolates show enzyme activity.
S. M. Taghavi, M. Ziaee,
Volume 7, Issue 1 (4-2003)
Abstract
In order to compare Pseudomonas syringae pv. syringae (Pss) isolates from cereals, citrus, stone fruits and weeds by phenotypic characteristics, serological properties and pathogenicity, samples were taken during 1998-1999 in Fars Province, Karaj, Fereidan, Aligudarz and Shahrekord regions. From 350 fluorescent isolates, 47 were negative with respect to oxidase, potato soft rot and arginine dihydrolase tests but positive in hypersensitive reaction (HR) on tobacco, pelargonium, or both. In complementary biochemical tests, these isolates were identified as Pss. Based on LOPAT (levan, oxidase, potato soft rot, arginine dihydrolase and HR on tobacco leaves) tests, the isolates were divided into two groups, but based on the results of GATTa tests alone, isolates were divided into nine groups. Isolates from different hosts showed differences in lipolysis, syringomycin production, ice nucleation activity, lecithinase, pathogenicity, serology and protein profiles.
M. R. Safari Motlagh, F. Padasht, Gh. Hedjaroude,
Volume 9, Issue 2 (7-2005)
Abstract
The brown spot disease is one of the seed-borne diseases of rice, found in the all stages of its growth from nursery to farm. It causes qualitative and quantitative damage on rice. Therefore, some studies carried out on the different aspects of this disease and the reaction of some cultivars of rice against the pathogenic fungous agent of the diseases. To do so, 120 isolates, isolated from plant samples were collected from the 91 paddy fields in Guilan. To isolate the pathogenic fungous agent of the disease, the collected samples were cultured on PDA and filter paper. According to the results, the present isolation related to Bipolaris with the 3 species : Bipolaris oryzae (Ito & Kurib.) Drechsler ex Dastur Bipolaris victoriae Nelson Bipolaris sp. The total isolataes were consisted of 15% Bipolaris oryzae, 75% Bipolaris -victoriae and 10% Bipolaris sp. The study of pathogenicity of isolation in these three species was applied to Khazar rice in desicator. The symptoms created by these three species were different from necrotic spots to leaf death. The results indicated that not only the symptoms but also the virulence in these three species were different. The virulence in B. oryzae was more than B. sp and this was more than B. victoriae. To study the reaction of some cultivars of rices to the pathogenic fungous agent, 8 cultivars of rice: “ Bejar, Khazar, Sepeedroud, Domsephid, Hassan saraee, Binam, Neda, Nemat” respectively were used in the two stages of the plant growth, to leaf in the seedling stage and heading. The results indicated that in seedling stage there was no significant difference between these cultivars with respect to the amount of infection. And in heading stage, according to “Dunken” test (5%) they divided into the 3 groups: “Neda, Sepeedrud, Khazar and Binam” with the lowest amount of infection were in the first group, “Bejar, Domsephid and Hassan saraee” classified in the second group and “Nemat” was in the third one. But there was no significant difference between them according to the variance analysis table.
