Volume 9, Issue 1 (spring 2005)
jwss 2005, 9(1): 103-111 |
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A. A. Ehsanpour, R. Taheri. Somatic Embryogenesis from Alfalfa (Medicago sativa L.) Callus Using Ethinyle Estradiol. jwss 2005; 9 (1) :103-111
URL: http://jstnar.iut.ac.ir/article-1-284-en.html
URL: http://jstnar.iut.ac.ir/article-1-284-en.html
Abstract: (18312 Views)
In this research the effect of ethinyl estradiol on somatic embryogenesis of Medicago sativa was investigated. Seeds were grown in vitro on MS medium containing 2,4-D, Kin and NAA for callus induction. Plant regeneration media with 14 different combinations of auxin, cytokinin and ethinyl estradiol were provided. Three to four-week old celli and stem or hypocotyl segments of Medicago sativa were transferred to regeneration media. Results showed that ethinyl estradiol (l mg/L) induced shoot, root and somatic embryo on calli. When the stem and hypocotyl segments were cultured on medium M12, only shoots and roots were produced with no somatic embryo. Finally, it was observed that ethinyl stradiol and auxin together could induce somatic embryogenesis and plant regeneration, while these hormones had no effect separately.
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