Volume 8, Issue 4 (winter 2005)
jwss 2005, 8(4): 41-46 |
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B. E. Sayed-Tabatabaei. Identification of AFLP Molecular Markers Linked to Row-Type Gene in Barley. jwss 2005; 8 (4) :41-46
URL: http://jstnar.iut.ac.ir/article-1-300-en.html
URL: http://jstnar.iut.ac.ir/article-1-300-en.html
Abstract: (23730 Views)
Formation of the two- and six-rowed types in barley is predominantly controlled by alleles at a single locus (vrs1) which is located in long arm of chromosome 2H. This gene is a key character on the study of barley domestication and yield. Near-isogenic lines (NILs) of barley were produced from crosses between Kanto Nakate Gold (tow-rowed) and Azumamugi (six-rowed). The selected lines were used for screening of AFLP polymorphic bands which are linked to vrs1 locus. After screening of a total of 1792 primer combination, five polymorphic bands were identified. A construction of high resolution map around the vrs1 locus was made using recombinant inbred lines. These markers can be used for a map-based cloning of the genes at the vrs1 locus.
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