JWSS - Journal of Water and Soil Science

Improvement of soils is among the major concerns in civil engineering, therefore a variety of approaches have been employed for different soil types. The annual budget of implementing the projects of this kind in countries clearly implies the importance of the subject. The loose granular soils and sediments have always imposed challenges due to their low strength and bearing capacity. Bio-mediated soil improvement has recently been introduced as a novel link of biotechnology (biotech) and civil engineering for improving the problematic soils, i.e. utilizing some bacteria to precipitate calcite on the soil particles. Bio-grouting is a branch of Bio-mediated soil improvement which is a method based on microbial calcium carbonate precipitation. In this regard, the soil samples were stabilized by injecting the bacterium Sporosarcina pasteurii in the first phase of the process and Urea and Calcium Chloride in the second phase of the process (two-phase injection) as the nutrients into the sandy soil columns and subjected to unconfined compressive strength test. In this research, Taguchi method was utilized for design of experience (DOE). Based on results obtained, the activity of the bacteria caused the precipitation of calcium carbonate in soil samples so that after 21 days, the unconfined compressive strength of the soil increased from 85 kPa in the control sample to 930 kPa at optimum condition.