Volume 8, Issue 2 (summer 2004)
jwss 2004, 8(2): 149-158 |
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M. Mohamadi Bazargani, B. E. S. Tabatabaei, A. Rezaei, C. Ghobadi. Optimizing Regeneration of Cotton Plant via Shoot Apex. jwss 2004; 8 (2) :149-158
URL: http://jstnar.iut.ac.ir/article-1-431-en.html
URL: http://jstnar.iut.ac.ir/article-1-431-en.html
Abstract: (19201 Views)
Optimizing regeneration of cotton plant in two variety (Sahel and Varamin) via shoot apex was done in order to Agrobacterium-mediated transformation. In this reaserch shoot apexes of two varieties were isolated from four or five day seedling and were placed on a special medium of shooting (modified MS without hormon).In order to select the best rooting media, The Statistical Analysis explants that produce shoot and leaves in a CRBD with 4 replicates and 4 rooting treatments: 1) modified MS without hormon, 2) ½ MS with 0.1 mg/lit IBA, 3) ½ MS with 0.1 mg/lit NAA, 4) ½ MS with 0.1 mg/lit IAA. The statistical analysis indicated that the best for both varieties, was medium with 0.1 mg/lit IBA and rooting percentage of Varamin is better than sahel in all of media.
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